Parasite-host network analysis provides insights into the evolution of two mistletoe lineages(Loranthaceae and Santalaceae)

Mistletoes are ecologically important parasitic plants,with> 1600 species from five lineages worldwide.Mistletoe lineages exhibit distinct patterns of species diversification and host specificity,however,the mechanisms underlying these differences are poorly understood.In this study,we analysed a comprehensive parasite-host network,including 280 host species from 60 families and 22 mistletoe species from two lineages(Santalaceae and Loranthaceae) in Xishuangbanna,located in a biodiversity hotspot of tropical Asia.We identified the factors that predict the infection strength of mistletoes.We also detected host specificity and the phylogenetic signal of mistletoes and their hosts.We found that this interaction network could be largely explained by a model based on the relative abundance of species.Host infection was positively correlated with diameter at breast height and tree coverage,but negatively correlated with wood density.Overall,closely related mistletoe species tend to interact more often with similar hosts.However,the two lineages showed a significantly different network pattern.Rates of host generality were higher in Loranthaceae than in Santalaceae,although neither lineage showed phylogenetic signal for host generality.This study demonstrates that the neutral interaction hypothesis provides suitable predictions of the mistletoe-host interaction network,and mistletoe species show significant phylogenetic signals for their hosts.Our findings also indicate that high species diversification in Loranthaceae may be explained by high rates of host generality and the evolutionary history shared by Loranthaceae species with diverse host plants in the tropics.

Phenotypic and genetic variation between two populations of the Chinese yellow pond turtle, Mauremys mutica (Cantor, 1842)

Mauremys mutica(Cantor,1842)is an endangered species in China.Main phenotypic variations inbody color,body weight,body shape,clutch size,egg size,and hatchling size were revealed betweenthe southern and northern populations.Both populations have the phenomenon of'larger male'sexualsize dimorphism(SSD),especially in the southern population.Furthermore,genetic variations betweenthe two populations were analyzed by RAPD band patterns of 30 random individuals in each population.The average genetic distance was 0.299±0.108 among the samples of two populations.The average ge-netic distance between southern and northern populations was 0.305±0.046.Cluster analysis indicatedthat all the individuals from the southern and northern populations were clustered among themselves toform two distinct clades.A total of 20 population-specific RAPD fragments were scored from 16 primers,and could be used as RAPD markers for distinguishing the southern and the northern population.Basedon the nucleotide sequences of two RAPD markers,two pairs of SCAR primers(SC1-S and SC2-S)weredesigned,which could be used as SCAR markers for the southern population.According to the significantphenotypic and genetic variations,we suggested that the northern population and southern populationmight be considered as two separate taxa,the'northern taxon'and the'southern taxon',and the con-servation should be respectively conducted on the two taxa.

Antitumor and immune regulation activities of the extracts of some Chinese marine invertebrates

Extracts of 21 marine invertebrates belonging to Coelenterata, Mollusca, Annelida, Bryozoa, Echiura, Arthropoda, Echinodermata and Urochordata were screened for the studies on their antitumor and immune regulation activities. Antitumor activity was determined by MTT method and immune regulation activity was studied using T- and B-lymphocytes in mice spleen in vitro. It was found that the n-butanol part of Asterina pectinifera, the acetic ether part of Tubuaria marina,95% ethanol extract of Acanthochiton rubrolineatus have a high inhibition rate of 96.7%,63.9% and 50.5% respectively on tumor cell line HL-60 at the concentration of 0.063 mg/ml. The inhibition rate of the acetic ether part of Tubuaria marina on the tumor cell line A-549 is 65.4 % at concentration of 0.063 mg/mL. The 95% ethanol extract of Meretrix meretrix has so outstanding promoting effect on T-lymphocytes that their multiplication increases 25% when the sample concentration is only 1 μg/ml. On B-lymphocytes, the 95% extract of Rapana venosa, at concentration of 100 μg/ml, has a promotion percent- age of 60%. On the other hand, under the condition of no cytotoxic effect, the 95% ethanol extracts of Acantho- chiton rubrolineatus and Cellana toreum can reach 92% inhibition rate on T lymphocyte at concentration of 100 μg/ml, while the inhibition rate on B lymphocyte of the 95% extract of Acanthochiton rubrolineatus reaches 92% at the same concentration.

Interannual Meridional Displacement of the East Asian Upper-tropospheric Jet Stream in Summer

On the interannual timescale, the meridional displacement of the East Asian upper-tropospheric jet stream (EAJS) is significantly associated with the rainfall anomalies in East Asia in summer. In this study, using the data from the National Centers for Environmental Prediction-Department of Energy (NCEP/DOE) reanalysis-2 from 1979 to 2002, the authors investigate the interannual variations of the EAJS's meridional displacement in summer and their associations with the variations of the South Asian high (SAH) and the western North Pacific subtropical high (WNPSH), which are dominant circulation features in the upper and lower troposhere, respectively. The result from an EOF analysis shows that the meridional displacement is the most remarkable feature of the interannual variations of the EAJS in each month of summer and in summer as a whole. A composite analysis indicates that the summer (June-July-August, JJA) EAJS index, which is intended to depict the interannual meridional displacement of the EAJS, is not appropriate because the anomalies of the zonal wind at 200 hPa (U200) in July and August only, rather than in June, significantly contribute to the summer EAJS index. Thus, the index for each month in summer is defined according to the location of the EAJS core in each month. Composite analyses based on the monthly indexes show that corresponding to the monthly equatorward displacement of the EAJS, the South Asian high (SAH) extends southeastward clearly in July and August, and the western North Pacific subtropical high (WNPSH) withdraws southward in June and August.

The response of lake-glacier variations to climate change in Nam Co Catchment, central Tibetan Plateau, during 1970-2000

Based upon the 1970 aero-photo topographic map, and TM/ETM satellite images taken in 1991 and 2000, the authors artificially interpreted boundaries of lake and glaciers in Nam Co Catchment, and quantified lake-glacier area variations in different stages by ＂integrated method＂ with the support of GIS. Results show that from 1970 to 2000, lake area increased from 1942.34 km^2 to 1979.79 km^2 at a rate of 1.27 km^2/a, while glacier area decreased from 167.62 km^2 to 141.88 km^2 at a rate of 0.86 km^2/a. The increasing rate of lake in 1991-2000 was 1.76 km^2/a that was faster than 1.03 km^2/a in 1970-1991, while in the same period of time, the shrinking rates of glaciers were 0.97 km^2/a and 0.80 km^2/a respectively. Important factors, relevant to lake and glacier response to the climate, such as air temperature, precipitation, potential evapotranspiration and their values in warm and cold seasons, were discussed. The result suggests that temperature increasing is the main reason for the accelerated melting of glaciers. Lake expansion is mainly induced by the increase of the glacier melting water, increase of precipitation and obvious decrease of potential evapotranspiration. Precipitation, evaporation and their linkages with lake enlargement on regional scale need to be thoroughly studied under the background of global warming and glacier retreating.

Using ^(137)Cs Tracer Technique to Evaluate Erosion and Deposition of Black Soil in Northeast China

Soil and water losses through erosion have been serious in the black soil region of Northeast China. Therefore, a sloping cultivated land in Songnen Plain was selected as a case study to: 1) determine the 137Cs reference inventory in the study area; 2) calculate erosion and deposition rates of black soil on different slope locations; 3) conduct a sensitivity analysis of some model parameters; and 4) compare overall outputs using four different models. Three transects were set in the field with five slope locations for each transect, including summit, shoulder-slope, back-slope, foot-slope, and toe-slope. Field measurements and model simulation were used to estimate a bomb-derived 137Cs reference inventory in the study area. Soil erosion and deposition rates were estimated using four 137Cs models and percentage of 137Cs loss/gain. The 137Cs reference value in the study area was 2 232.8 Bq m-2 with 137Cs showing a clear topographic pattern, decreasing from the summit to shoulder-slope, then increasing again at the foot-slope and reaching a maximum at the toe-slope. Predicted soil redistribution rates for different slope locations varied. Among models, the Yang Model (YANG-M) overestimated erosion loss but underestimated deposition. However, the standard mass balance model (MBM1) gave predictions similar to a mass balance model incorporating soil movement by tillage (MBM2). Sensitivity analysis of the proportion factor 7 and distribution pattern of 137Cs in the surface layer demonstrated the impact of 137Cs enrichment on calculation of the soil erosion rate. Factors influencing the redistribution of fallout 137Cs in landscape should be fully considered as calculating soil redistribution rate using 137Cs technique.

Distribution of Soil Enzyme Activities and Microbial Biomass Along a Latitudinal Gradient in Farmlands of Songliao Plain,Northeast China

Soil enzymes activities and microbial biomass have an important influence on nutrient cycling. The spatial distribution of soil enzymes activities and microbial biomass were examined along a latitudinal gradient in farmlands of Songliao Plain, Northeast China to assess the impact of climatic changes along the latitudinal transect on nutrient cycling in agroecosystems. Top soils (0-20 cm depth) were sampled in fields at 7 locations from north (Hailun) to south (Dashiqiao) in the end of October 2005 after maize harvest. The contents of total C, N, and P, C/N, available N, and available P increased with the latitude. The activities of invertase and acid phosphatase, microbial biomass (MB) C and N, and MBC/MBN were significantly correlated with latitude (P < 0.05, r2 = 0.198, 0.635, 0.558, 0.211 and 0.317, respectively), that is, increasing with the latitude. Significant positive correlations (P < 0.05) were observed between invertase activity and the total N and available P, and between acid phosphatase activity and the total C, C/N, available N, total P and available P. The urease, acid phosphatase, and dehydrogenase activities were significantly correlated with the soil pH and electrical conductivity (EC) (P < 0.05). MBC and MBN were positively correlated with the total C, C/N, and available P (P < 0.05). The MBC/MBN ratio was positively correlated with the total C, total N, C/N, and available N (P < 0.05). The spatial distribution of soil enzyme activities and microbial biomass resulted from the changes in soil properties such as soil organic matter, soil pH, and EC, partially owing to variations in temperature and rainfall along the latitudinal gradient.

Short-term Effects of Tillage Practices on Organic Carbon in Clay Loam Soil of Northeast China

一个耕种实验，由犁板组成犁( MP )，山脉耕种( RT )，和没有耕种( NT )，与四在一个使随机化的完全的块图案被执行复制在东北中国在粘壤土土壤的表面层( 30 厘米)在 SOC 内容和它的分发上学习3年的耕种管理的效果。NT 没与 MP 和 RT 相比在表层土(0 5 厘米) 导致 SOC 的重要增加；然而，在 NT 土壤的 SOC 内容显著地在 5 20 厘米的深度被减少。因此，短期(3 年) NT 管理趋于成层 SOC 集中，然而并非必然增加它在为土壤的犁层的存储。

Characteristics of grassland degradation and driving forces in the source region of the Yellow River from 1985 to 2000

The source region of the Yellow River is located in the middle east of the Tibetan Plateau in northwest China. The total area is about 51,700 km^2, mainly covered by grassland （79%）, unused land （16%） and water （4%）. The increasing land utilization in this area has increased the risk of environmental degradation. The land use/cover data （1985 and 2000） provided by the Data Center of Resources and Environment in the Chinese Academy of Sciences were used to analyze the land cover change in the source region of the Yellow River. DEM （1：250,000） data, roads and settlement data were used to analyze the spatial characteristics of grasslands degradation. The ArcGIS 9 software was used to convert data types and do the overlay, reclassification and zonal statistic analysis. Results show that grassland degradation is the most important land cover change in the study area, which occupied 8.24% of the region＇s total area. Human activities are the main causes of the grassland degradation in the source region of the Yellow River： 1） the degradation rate is higher on the sunny slope than on the shady slope; 2） the grassland degradation rate decreases with an increase in the elevation, and it has a correlation coefficient of -0.93; 3） the nearer to the settlements the grassland is, the higher the degradation rate. Especially within a distance range of 12 km to the settlements, the grassland degradation rate is highly related with the distance, with a coefficient of -0.99; and 4） in the range of 4 km, the degradation rate decreases with the increase of distance to the roads, with a correlation coefficient of -0.98. Besides some physical factors, human activities have been the most important driving forces of the grassland degradation in the source region of the Yellow River since 1985. To resolve the degradation problems, population control is essential, and therefore, it can reduce the social demand of livestock products from the grassland. To achieve sustainable development, it needs to improve the management of grassland ecosystem.

Spatial Prediction of Heavy Metal Pollution for Soils in Peri-Urban Beijing, China Based on Fuzzy Set Theory

Fuzzy classification combined with spatial prediction was used to assess the state of soil pollution in the peri-urban Beijing area. Total concentrations of As, Cr, Cd, Hg, and Pb were determined in 220 topsoil samples (0-20 cm) collected using a grid design in a study area of 2600 km2. Heavy metal concentrations were grouped into three classes according to the optimum number of classes and fuzziness exponent using the fuzzy c-mean (FCM) algorithm. Membership values were interpolated using ordinary kriging. The polluted soils of the study area induced by the measured heavy metals were concentrated in the northwest corner and eastern part, especially the southeastern part close to the urban zone, whereas the soils free of pollution were mainly distributed in the southwestern part. The soils with potential risk of heavy metal pollution were located in isolated spots mainly in the northern part and southeastern corner of the study region. The FCM algorithm combined with geostatistical techniques, as compared to conventional single geostatistical kriging methods, could produce a prediction with a quantitative uncertainty evaluation and higher reliability. Successful prediction of soil pollution achieved with FCM algorithm in this study indicated that fuzzy set theory had great potential for use in other areas of soil science.

PRMT5 regulates Golgi apparatus structure through methylation of the golgin GM130

Golgi 仪器(GA ) 的维护结构和功能取决于 Golgi 矩阵蛋白质。象 golgin 和掌握家庭的成员的 phosphorylation 那样的 Golgi 蛋白质的 posttranslational 修正为决定 Golgi 建筑学是重要的。包括 golgin-84 的一些 Golgi 蛋白质也被知道是 methylated，但是 golgin methylation 的功能仍然保持不清楚。这里，我们证明蛋白质精氨酸 methyltransferase (PRMT5 ) 5 本地化到 GA 和形式建筑群，几个部件在拴住的 GA 带子形成和泡包含了。PRMT5 在 Golgi 带子形成与 PRMT5 原因缺点的 golgin GM130，和弄空交往。而且，在 GM130 的 PRMT5 甲醇化物 N 终端精氨酸，和如此的精氨酸 methylation 为 GA 显得批评带子形成。我们的调查结果揭示 GM130 的 PRMT5 依赖的精氨酸 methylation 由控制 GA 建筑学的维护的分子的机制。

Effect of heavy metals on soil nematode communities in the vicinity of a metallurgical factory

The influence of Cu and Zn on soil nematode communities was examined along a pollution gradient with increasing distance from a metallurgical factory. Total and available heavy metal contents were used to study the effects of heavy metals on nematode abundance, trophic groups and ecological indices. The results demonstrated significant correlations between the number of total nematodes, bacterivores, plant-parasites and the total and available heavy metals. Bacterivores and plant-parasites were the dominant trophic groups. Significant differences in different sampling sites were found only in the number of bacterivores（P〈0.01）. The Shannon-Weaver diversity index（W）, trophic diversity index（TD）, evenness index（J＇） and dominance index（λ） were found to be sensitive to soil pH and C/N ratios. Significant correlations were found between the total nematodes （TNEM）, some genera （A crobeloides, Aphelenchoides, Cephalobus, Ditylenchus, Mesorhabditis, Tetylenchus and Tylenchus） and distance from the factory.

Distribution characteristics of dissolved organic carbon in annular wetland soil-water solutions through soil profiles in the Sanjiang Plain,Northeast China

Overwhelming evidence reveals that concentrations of dissolved organic carbon （DOC） have increased in streams which brings negative environmental impacts. DOC in stream flow is mainly originated from soil-water solutions of watershed. Wetlands prove to be the most sensitive areas as an important DOC reserve between terrestrial and fluvial biogeosystems. This reported study was focused on the distribution characteristics and the controlling factors of DOC in soil-water solutions of annular wetland, i.e., a dishing wetland and a forest wetland together, in the Sanjiang Plain, Northeast China. The results indicate that DOC concentrations in soilwater solutions decreased and then increased with increasing soil depth in the annular wetland. In the upper soil layers of 0-10 cm and 10-20 cm, DOC concentrations in soil-water solutions linearly increased from edge to center of the annular wetland （R^2 = 0.3122 and R^2 = 0.443）. The distribution variations were intimately linked to DOC production and utilization and DOC transport processes in annular wetland soil-water solutions. The concentrations of total organic carbon （TOC）, total carbon （TC） and Fe（II）, DOC mobility and continuous vertical and lateral flow affectext the distribution variations of DOC in soil-water solutions. The correlation coefficients between DOC concentrations and TOC, TC and Fe（II） were 0.974, 0.813 and 0.753 respectively. These distribution characteristics suggested a systematic response of the distribution variations of DOC in annular wetland soil-water solutions to the geometry of closed depressions on a scale of small catchments. However, the DOC in soil pore water of the annular wetland may be the potential source of DOC to stream flow on watershed scale. These observations also implied the fragmentation of wetland landscape could bring the spatial-temporal variations of DOC distribution and exports, which would bring negative environmental impacts in watersheds of the Sanjiang Plain.

Effects of Terracing and Agroforestry on Soil and Water Loss in Hilly Areas of the Sichuan Basin, China

Soil erosion in hilly areas of the Sichuan Basin is a serious concern over sustainable crop production and sound ecosystem. A 3-year experiment was conducted using the method of runoff plots to examine the effects of terracing and agroforestry in farmland systems on soil and water conservation of slope fields in the hilly areas in Jianyang County, Sichuan Province, Southwestern China. A power function (Y = aXb) can statistically describe the relationship between water runoff (Y) and rainfall (X). The regression equation for the treatment of sloping terraces with crops (Plot 2) is remarkably different from that for the treatment of sloping terraces with grasses and trees (Plot 1) and the conventional up- and down-slope crop system (Plot 3) regarding equation coefficients, while regression equations are similar between Plot 1 and Plot 3. Water runoff amount and runoff coefficient of slope fields increased by 21.5～41.0 % and 27.5～69.7 % respectively, compared to those of sloping terraces, suggesting that terracing notably reduced the water runoff in the field. In the case of sloping terraces, lower amount of water runoff was observed on sloping terraces with crops than on sloping terraces with grasses and trees. Sediment yields on the slope fields in the normal year of rainfall distribution were notably higher (34.41～331.67 % and 37.06～403.44 % for Plot 1 and Plot 2, respectively) than those on sloping terraces, implying that terracing also plays a significant role in the reduction in soil erosion. It is suggested that terracing with crops is significantly effective for soil and water conservation in cultivatedfarmland, while the conventional practice of up- and down- slope cultivation creates high rates of water runoff and soil sediment transport. Terracing with grasses and fruit trees shows a less reduction in water runoff than terracing with crops, which was observed in the 3-year experiments.

Single and joint effects of petroleum hydrocarbons and cadmium on the polychaete Perinereis aibuhitensis Grube

Using the concentration gradient and combined pollutant exposure method, the single and joint effects of petroleum hydrocarbons （PHCs） and cadmium （Cd） on polychaete Perinereis aibuhitensis Grube, an ecologically keystone species in estuarine and coastal environment, have been investigated. The results indicate that the toxicity of PHCs to P. aibuhitensis is stronger than that of Cd to the organism. There are positive correlations between the mortality of worms and the exposed concentration of single Cd or PHCs in solution. Similarly, the accumulation of Cd or PHCs in worms increased with increasing Cd- or PHC-exposed concentrations. All the correlation relationships can be described using unitary quadratic equations （Y or Z = aX^2 ＋ bX ＋ c）. It is calculated, on the basis of these expressions, that the median lethal dose （LC50） ofP aibuhitensis exposed to a single Cd or PHCs is 793.4-13567.3 and 28.0-119.9 μg/L, respectively. The exposed time has some stimulative effect on the two pollutants and on the mortality of the worms. Thus, even a low concentration of a single Cd or PHCs may have strong toxic effects on the worms when the exposed time becomes longer. The accumulation of Cd or PHCs in worms differs with an increase in exposure time at the given exposed concentration of a single Cd or PHCs. Noticeably, the accumulation of PHCs in worms decreases with an increase in exposure time at the given high concentration of PHCs in solution. The joint effect of PHCs and Cd on P. aibuhitensis is very complicated and changes with the exposed concentrations of the two pollutants. At the given concentration of PHCs, the joint toxicity of the two pollutants on the worms changes from synergism to antagonism with an increase in Cd concentration. The accumulation of Cd in the worms significantly decreases with the addition of PHCs to exposure solution.

The Tunggurian Stage of the Continental Miocene in China

The Tunggurian Age was nominated in 1984, and the Second National Commission on Stratigraphy of China formally suggested establishing the corresponding chronostratigraphic unit, the Tunggurian Stage, based on the Tunggurian Age in 1999. The name of this stage comes from a lithostratigraphic unit, the Tunggur Formation, and the stratotype section is located at the Tunggur tableland, 15 km southeast of Saihan Gobi Township, Sonid Left Banner, Inner Mongolia. The Tunggurian Age is correlated to the Astaracian of the European land mammal ages, and they share the same definition of the lower boundary at the base of the paleomagnetic Chron C5Bn.1r with an age of 15.0 Ma. In the Tairum Nor section on the southeastern edge of the Tunggur tableland, this boundary is situated within the successive deposits of reddish-brown massive mudstone of the lower part of the Tunggur Formation, with a distance of 7.6 m from the base of the grayish-white sandstones in the middle part of the section. The Tunggurian is approximately correlated to the upper part of the marine Langhian and the marine Serravallian in the International Stratigraphical Chart. The Tunggurian Stage includes two Neogene mammal faunal units, i.e. NMU 6 （MN 6） and NMU 7 （MN 7/8）. The Tairnm Nor fauna from the Talrnm Nor section corresponds to NMU 6, and the Tunggur fauna （senso stricto） from the localities on the northwestern edge of the Tunggur tableland, such as Platybelodon Quarry, Wolf Camp and Moergen, corresponds to NMU 7. Among the Middle Miocene mammalian faunas in China, the Laogou fauna from the Linxia Basin, Gansu, the Quantougou fauna from the Lanzhou Basin, Gansu, the Halamagai fauna from the northern Junggar Basin, Xinjiang, and the Dingjiaergou fauna from Tongxin, Ningxia correspond to NMU 6.

Dynamics of soil inorganic nitrogen and their responses to nitrogen additions in three subtropical forests, south China

Three forests with different historical land-use, forest age, and species assemblages in subtropical China were selected to evaluate current soil N status and investigate the responses of soil inorganic N dynamics to monthly ammonium nitrate additions. Results showed that the mature monsoon evergreen broadleaved forest that has been protected for more than 400 years exhibited an advanced soil N status than the pine （Pinus massoniana） and pine-broadleaf mixed forests, both originated from the 1930＇s clear-cut and pine plantation. Mature forests had greater extractable inorganic N pool, lower N retention capacity, higher inorganic N leaching, and higher soil C/N ratios. Mineral soil extractable NH4^＋-N and NO3-N concentrations were significantly increased by experimental N additions on several sampling dates, but repeated ANOVA showed that the effect was not significant over the whole year except NH4^＋-N in the mature forest. In contrast, inorganic N （both NH4^＋-N and NO3^--N） in soil 20-cm below the surface was significantly elevated by the N additions. From 42% to 74% of N added was retained by the upper 20 cm soils in the pine and mixed forests, while 0%-70% was retained in the mature forest. Our results suggest that land-use history, forest age and species composition were likely to be some of the important factors that determine differing forest N retention responses to elevated N deposition in the study region.

New Evidence for Magnetic Reconnection in the Tail of Interplanetary Magnetic Cloud

We analyse the WIND data of an interplanetary magnetic cloud （MC） on 2 November 2001, and find new evidences for magnetic reconnection in the tail of this MC. In the MC tail, the largely dip and the large change of the orientation of the magnetic field occurred simultaneously, △θ≈45° and △φ changed from 90° to 320°. Correspondingly, the number density of ions increased, and the superthermal electrons were heated and accelerated, however its number density decreased. Meanwhile, inverse jets and Hall term were observed. The pitch-angle distributions of the electrons with lower energy and higher energy showed strong turbulence and bi-direction flow, respectively. The plasma wave activity enhanced near the electron plasma frequency, fpe and 2fpe. These important physical characteristics are new evidences for magnetic reconnection existing in interplanetary space.

Different roles of Ekman pumping in the west and east segments of the South China Sea Warm Current

A three-dimensional model is used to investigate the mechanism of the South China Sea （SCS） winter counter-current （also known as the SCS Warm Current, or the SCSWC）, which flows against the wind. The model can reproduce the structure of the band-like currents over the northern shelf of the SCS, including the westward coastal current and slop current, and the SCSWC sandwiched in between. Sensitivity experiments are designed to understand the different roles of Ekman pumping of the SCSWC at different longitude. The results show that the Ekman pumping drives the SCSWC in the west segment. In the east, it is not the Ekman pumping but the intrusion of the Kuroshio that drives the SCSWC.

Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil

Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis （DGGE） and sequencing approaches in a microcosm experiment. Changes of soil nifH gene diversity and composition were examined following the application of acetochlor, methamidophos and their combination. Acetochlor reduced the nifH gene diversity （both in gene richness and diversity index values） and caused changes in the nifH gene composition. The effects of acetochlor on nifH gene were strengthened as the concentration of acetochlor increased. Cluster analysis of DGGE banding patterns showed that nifH gene composition which had been affected by low concentration of acetochlor （50 mg/kg） recovered firstly. Methamidophos reduced nifH gene richness that except at 4 weeks. The medium concentration of methamidophos （150 mg/kg） caused the most apparent changes in nifH gene diversity at the first week while the high concentration of methamidophos （250 mg/kg） produced prominent effects on nifH gene diversity in the following weeks. Cluster analysis showed that minimal changes of nifH gene composition were found at 1 week and maximal changes at 4 weeks. Toxic effects of acetochlor and methamidophos combination on nifH gene were also apparent. Different nifH genes （bands） responded differently to the impact of agrochemicals： four individual bands were eliminated by the application of the agrochemicals, five bands became predominant by the stimulation of the agrochemicals, and four bands showed strong resistance to the influence of the agrochemicals. Fifteen prominent bands were partially sequenced, yielding 15 different nifH sequences, which were used for phylogenetic reconstructions. All sequences were affiliated with the alpha- and beta-proteobacteria, showing higher similarity to eight different diazotrophic genera.