Application of ARFI technology to explore the clinical study of Gandou Tang in treating liver fibrosis of wilson's disease with damp-heat accumulation

Objective:Acoustic radiation force impulse(ARFI)was applied to measure Shear wave velocity(SWV)of liver in patients with Wilson's disease(WD).To investigate the relationship between SWV and the serological indexes of liver fibrosis,such as type Ⅳ collagen(CⅣ),hyaluronic acid(HA),type Ⅲ procollagen peptide(PⅢNP),laminin(LN),APRI score(Asparate Aminotransfer to Platelet Ratio Index),and FIB-4 index(FIB-4 index).The clinical efficacy of GandouTang(GDT)in the treatment of liver fibrosis in WD with damp-heat internalization was also observed.Methods:80 cases of WD patients who met the inclusion criteria were randomly divided into the treatment group and the control group,with 40 cases in each group.The control group was treated with Sodium Dimercaptopropylsulfonate(DMPS)and the treatment group was additionally treated with the traditional Chinese medicine GDT.One course for 8 days,a total of 6 courses.The levels of SwV and four serological indicators of liver fibrosis(PⅢNP,HA,CⅣ,LN),APRI score and FIB-4 index were compared before and after treatment.Pearson correlation test was used to analyze the correlation between SWV and HA,CⅣ,LN,PⅢNP,APRI score and FIB-4 index.The effects of GDT on SWV,liver fiber,APRI and FIB-4 were evaluated according to the treatment plan.Results:①The SWV was positively correlated with FIB-4(r=0.83),APRI(r=0.82),HA(r=0.87),CⅣ(r=0.71),LN(r=0.85)and PINP(r=0.77).②Before treatment,there were no significant differences in SWV level,PⅢNP,HA,CⅣ,LN,APRI and FIB-4 levels between two groups(P>0.05).After treatment,the levels of PⅢNP,HA,LN,SWV,APRI and FIB-4 in both groups were significantly decreased(P<0.05,P<0.01),and the levels in the treatment group were lower than those in the control group.There were no significant specific changes in CⅣ level(P>0.05).Conclusion:SWV value can reflect the degree of WD liver fibrosis,and is positively correlated with HA,PⅢNP,CⅣ,LN,FIB-4 index and APRI score.On the basis of the treatment of protecting liver and expelling copper with western medicine,plus the treatment of traditional Chinese medicine GDT can effectively improve the degree of liver fibrosis in WD patients with damp-heat accumulation.

Thoughts on the Reform of Ideological and Political Teaching of “Tuina Manipulation”

Based on the current problems in the teaching of“Tuina Manipulation”course in our school,and the requirements of ideological and political teaching reform in the background of the new era,on the basis of consulting related literature and experience of actual teaching conditions,exploring the integration of ideological and political education elements into massage An effective way of teaching courses,put forward the ideas of Tuina ideological and political teaching reform.Ultimately,it will achieve the goal of“integrated”teaching of basic tuina courses,professional skills practice,moral quality improvement,comprehensive quality training,and training a group of high-quality and high-level Chinese medicine talents that meet the needs of social development.

Interpretation of diagnosis and treatment scheme of COVID-19 in Hunan province (the third trial version) based on three factors and measures

The similarities and differences between"Novel Coronavirus Infected pneumonia in Hunan province(trial second edition)"and"Novel Coronavirus Infected pneumonia(Trial seventh edition)"released by the National Health Commission and their ideas were interpreted based on"suiting measures to three factors".①In Hunan program,the third version of the trial adjustment more suitable for local characteristics of the disease;②The prevention program population classification is clear,support,eliminate pathogenic factors of the primary and secondary distinct;③The cause of disease is prominent,damp and toxin are mixed with dryness,and the treatment is mainly to moisten the lung and clear away heat,and the prescription is mostly Xinliang light agent.

Based on data mining to explore the medication rule of Professor Shao Zheng-bin in the treatment of coronary heart disease combined with type2 diabetes mellitus after PCI

Objective:To explore the clinical medication rule of Shao Zhengbin in the treatment of coronary heart disease with type 2 diabetes mellitus after PCI by using data mining technology.Methods:Shao Zhengbin was collected from January 2016 to may 2019 in the outpatient department of the First Affiliated Hospital of Anhui University of traditional Chinese medicine to treat patients with coronary heart disease combined with type 2 diabetes mellitus after PCI.The data base was established with Microsoft Excel 2016,SPSS statistic 24.0,SPSS modeler 18.0 computer software,and drug frequency analysis,high-frequency drug association rule analysis and clustering were carried out Analysis and factor analysis.Results:of the 133 prescriptions included in the study,86 Chinese herbs were involved,and the top 10 drugs were dangshen,Huangjing,Danshen,Gualou,chuanxiong,fried Atractylodes rhizome,Poria cocos,Fushen and Chenpi respectively;12 drug associations were generated by association analysis,including Huangjing,Huangjing,chuanxiong,dandelion,Dangshen and banpi;12 drug associations were obtained by cluster analysis,including Huangjing,huangxiong,Gualou and Huangqi There are 7 clustering formulas,such as Xia,Danshen,Fuling,Gualou,etc.Conclusion:Shao Zhengbin is good at the treatment of coronary heart disease combined with type 2 diabetes mellitus after PCI.

Effect of sinew-regulating and bone-setting manipulating combined with exercise training in the treatment of joint injury of distal radio-ulnar

Objective: To observe the effect of the joint injury of the distal radio-ulnar joint. Methods: 60 patients with Distal Radioulnar Joint (DRUJ) injury were divided into observation group and control group according to random number method. 30 cases were included in each of the two groups.Before and after treatment in patients with Visual Analogue Scale (Visual Analogue Scale, VAS) score, forearm pronation and supination electromyographic activity, methods of electric integral value (integral electromyogram, iEMG) and Wrist in patients with self assessment Scale (Patient - Rated Wrist Evaluation, PRWE) score evaluation, comparison, and the clinical observation on diagnosis of disease and curative effect of traditional Chinese medicine standard (assessment process by blind method).Results: compared with the two groups before and after treatment, VAS score decreased, forearm pronation and postpronation activity increased, iEMG value increased, and PRWE scale score decreased (all P < 0.05), and the curative effect of the treatment group was better than that of the control group (P < 0.05). The total effective rate of the treatment group [93.3% (28/30)] was higher than that of the control group [50%(15/30), P < 0.05].Conclusion: the combined exercise training of muscle and bone setting technique can effectively alleviate the pain of patients with radial ulnar joint injury, improve the rotation of the forearm, increase the recruitment of the anterior rotatory muscle, and improve the wrist function of patients, and the effect is better than if combined with forearm support fixation.

Exploration of Professor Liu Taofeng's experience in the treatment of eczema based on Data Mining Technology

Objective: To explore the clinical medication law of Professor Liu Taofeng in the treatment of eczema by using data mining technology. Methods: the cases of eczema treated by Professor Liu Taofeng in the outpatient department of the First Affiliated Hospital of Anhui University of traditional Chinese medicine from June 2018 to October 2019 were collected and sorted out. The database was established with the help of Microsoft Excel 2016, SPSS statistical 24.0 and SPSS modeler 18.0 computer software, and the frequency analysis, high-frequency drug association rule analysis and cluster analysis were carried out. Results: among the 255 prescriptions included in the study, 41 traditional Chinese medicines were involved, and the top 10 drugs were fresh white skin, cortex Scutellariae, Cortex Moutan, Tribulus terrestris, Sophora flavescens, Salvia miltiorrhiza, Atractylodes macrocephala, Poria cocos, liquorice, and Cynanchum paniculatum;10 pairs of 2 drugs were associated by association analysis, such as "Tribulus terrestris → fresh white skin, Sophora flavescens → cortex Scutellariae";and "Poria cocos, Atractylodes macrocephala, lentils" were obtained by cluster analysis. Conclusion: Professor Liu Taofeng paid more attention to the heart and liver in the treatment of eczema, taking clearing away heat, cooling blood and removing dampness as the main treatment, and paid more attention to invigorating the spleen and stomach or removing blood stasis.

Use of Xinfeng capsule to treat abarticular pathologic changes in patients with rheumatoid arthritis

OBJECTIVE: To observe the influence of Xinfengcapsule(XFC) on abarticular pathologic changes(APCs) and other indices of patients with rheumatoid arthritis(RA) and explore the mechanism of action of XFC in improving such changes.METHODS: Three-hundred RA patients were divided randomly into a treatment group(n=150) and control group(n=150). A normal control(NC)group(n=90) was also created. Changes in cardiac function, pulmonary function, anemia indices and platelet parameters of RA patients were measured.Curative effects of the two groups were compared,and comparison carried out with the NC group.RESULTS: In 300 RA patients, late diastolic peak flow velocity(A peak) was much higher(P<0.01)and early diastolic peak flow velocity(E peak), E/A,and left ventricular fraction shortening much lower(P<0.01) than those in the NC group. Vital capacity(VC), forced vital capacity in one second, forced vital capacity(FVC), maximal voluntary ventilation(MVV), maximal expiratory flow in 50% of VC(FEF50) and FEF75 were lowered remarkably(P<0.05 or P<0.01). Platelet count(PLT), plateletcrit(PCT) and mean platelet volume(MPV) increased markedly(P<0.05 or P<0.01), and hemoglobin(Hb)level decreased significantly(P<0.05). After XFC treatment, the A peak and PLT and PCT were much lower(P<0.05), and E/A and the number of red blood cells as well as Hb level were much higher(P<0.05), as were FVC, MVV and FEF50(P<0.05 or P<0.01), in the treatment group than those in the NC group. Total score of pain and swelling in joints,uric-acid level and high-sensitivity C-reactive protein level were much lower, and superoxide dismutase level as well as the number of CD4 +CD25+ regulation T cells(Treg) and CD4+CD25+CD127- Treg were much higher(P<0.05 or P<0.01)in the treatment group than those in the NC group.CONCLUSION: RA patients with pathologic changes in joints also suffer from lower cardiac and pulmonary functions and from parameters of anemia and platelet factors. XFC can improve the symptoms of RA patients, ameliorate their cardiac and pulmonary functions and reduce the parameters of anemia and platelet factors. XFC lowers the immune inflammatory reaction to improve APCs in RA patients.

Effect of moxibustion on long-term memory in vascular dementia model rats

Objective: To observe the effects on spatial long-term memory in the rats with vascular dementia(VD)treated with moxibustion for resolving stasis and promoting meridian circulation.Methods: The modified 2-vascular occlusion(2-VO) method was used to prepare VD animal model. The rats were randomized into a sham-operation group, a model group, a moxibustion group and a western medication group, 15 rats in each one. In the moxibustion group, mild warm moxibustion was applied to"Bǎihuì(百会 GV 20)" "DàZhuī(大椎 GV 14)" and "Shéntíng(神庭 GV 24)". 20 min at each point, once a day, for 3 weeks consecutively. In the western medicaion group, mouse nerve growth factor(NGF) was injected intraperitoneally, 0.18 mL/kg, once a day, for 3 weeks consecutively. Morris water maze test was used, the time of the first passing platform, the frequency of passing-platform and swimming speed in120 s were determined of the rats in each group. Western blot were used to test the protein expressions of hippocampal Nestin and DCX.Results: At the end of three courses of treatment, compared with sham-operation group, the difference was not significant statistically in swimming speed in the model group, the moxibustion group and the western medication group separately(all P>0.05). Three days after modeling, the mean of escape latency was prolonged obviously in the modeled rats compared with the rats in the shamoperation group(88.84 ± 19.94 vs 18.15 ±9.41, P< 0.01). At the end of three courses of treatment, compared with the sham-operation group(22.01 ± 10.07), the mean of escape latency was different statistically in the model group(89.18± 19.70), the moxibustion group(37.21 ±13.31) and the western medication group separately(51.50± 16.15), all P<0.01. Compared with the model group, the mean of escape latency was shortened in the moxibustion group and western medication group respectively(37.21 ± 13.31 vs89.18 ± 19.70, 51.50 ± 16.15 vs 89.18 ± 19.70, both P< 0.01). Compared with the western medication group,there was no statistical significant difference in the mean of escape latency in the moxibustion group(37.21 ± 13.31 vs 51.50 ±16.15, P> 0.05). Compared with the sham-operation group, the frequency of passing-platform was decreased in the model group(0.73 ±0.96 vs 2.60 ±1.45, P<0.01); compared with model group, the frequency of passing-platform was increased in the moxibustion group and the western medicine group and the statistically significant difference presented in the moxibustion group(2.06 ± 1.33 vs 0.73 ±0.96, P<0.01). Compared with the sham-operation group, the time of first passing-platform was prolonged in the model group(87.86 ± 33.25 vs 36.13 +29.76, P< 0.01). Compared with the model group,the time of first passing-platform was shortened in the moxibustion group and the western medicine group respectively(49.53 ±29.48 vs 87.86 ± 33.25.P< 0.01; 58.98 ± 36.22 vs 87.86 ±33.25, P< 0.05). After three courses of treatment, compared with the sham-operation group, Nestin expression was reduced in the model group,(0.33 ±0.12 vs 0.51 ±0.02, P<0.05) and was increased in the moxibustion group and the western medication group respectively(1.33 ± 0.17 vs 0.51 ± 0.02. 1.39 ± 0.10 vs 0.51 ±0.02, both P< 0.01).Compared with the model group, Nestin expression was increased in the moxibustion group and the western medication group, respectively(1.33 ± 0.17 vs 0.33±0.12. 1.39±0.10 vs 0.33 ±0.12, both P< 0.01).Compared with the western medication group, the difference was not significant statistically in the moxibustion group(1.33±0.17 vs 1.39±0.10, P>0.05). After three courses of treatment, compared with the sham-operation group, DCX expression was decreased in the model group, but without significant difference(0.44±0.20 vs 0.51 ±0.26, P> 0.05), the expression was increased in the moxibustion group and the western medication group respectively(0.98±0.25 vs 0.51±0.26. P< 0.05; 1.11±0.43 vs 0.51 ±0.26,P<0.01). Compared with the model group, DCX expression was obviously increased in the moxibustion group and the western medication group(0.98 ± 0.25 vs 0.44 ± 0.20. 1.11 ± 0.43 vs 0.44 ± 0.20, both P<0.01). The difference in DCX expression was not statistically significant between the western medication group and the moxibustion group(0.98 ± 0.25 vs 1.11 ± 0.43. P> 0.05).Conclusion: Moxibustion apparently improves the long-term memory in VD rats.

The effect of moxibustion on cognitive attention network function of patients with mild cognitive impairment

Objective:To explore the effect differences between moxibustion and donepezil hydrochloride on the attention network function of patients with mild cognitive impairment(MCI).Methods:A total of 64 patients of MCI were randomly divided into the moxibustion group and donepezil hydrochloride group,32 cases in each one.On the basis of conventional treatment,the patients in the moxibustion group were given moxibustion,6 times a week,and the patients in the donepezil hydrochloride group were given donepezil hydrochloride orally,5 mg/day.The course of treatment was 60 days for both of the groups.Cognitive attention network function and activities of daily living(ADL)score were examined before and after treatment.Results:The differences of alerting reaction time(RT),executive control RT,overall mean RT and accuracy of the moxibustion group after treatment were significantly higher than those of the donepezil hydrochloride group[alert:(60.3±3.3)ms vs(48.3±3.7)ms,P<0.05;executive control:(81.2±3.2)ms vs(91.7±4.2)ms,P<0.05;total reaction time:(500.4±17.2)ms vs(536.2±20.1)ms.P<0.05;accuracy:(83.7±4.6)%vs(77.4±4.3)%,P<0.05].After treatment,the ADL scores of the both groups were significantly higher than those before treatment[the moxibustion group:(56.47±4.02)points vs(41.53±4.06)points,P<0.05;the donepezil hydrochloride group:(50.75±4.05)points vs(40.84±3.67)points,P<0.05],and the ADL score of the moxibustion group was significantly higher than that of the donepezil hydrochloride group[(56.47±4.02)points vs(50.75±4.05)points,P<0.05].Conclusion:Compared with donepezil hydrochloride,moxibustion has a better effect on the cognitive function of MCI patients.

Effect and safety of Shengxuening (extract from excrement of bombyxin) for renal anemia： a systematic review

OBJECTIVE: To assess the effect and safety of Shengxuening(SXN), extract from excrement of bombyxin, in the treatment of renal anemia, compared to ferrous succinate and ferrous sulfate.METHODS: According to the participant, intervention, comparison, outcomes, study design(PICOS)principles, we searched the Chinese Biomedical Literature Database, China National Knowledge Infrastructure Database, Chinese Evidence-Based Medicine Database, Wanfang Database(From establishment to December 2014). Two reviewers selected articles independently according to the inclusion and exclusion criteria. The quality of included studies was assessed by using the Cochrane Handbook.All statistical analyses were conducted by using Revman(vision 5.2) software.RESULTS: A total of 14 randomized controlled trials(RCTs) were enrolled in the review. The results revealed that, when compared with blank group, SXN significantly improved the hemoglobin(P >) levels[MD = 6.29, 95% CI(1.65-10.94), P < 0.0008] and albumin(ALB) [MD = 10.98, 95% CI(6.97-14.99), P <0.00001]. In addition, SXN could significantly increase the P > levels [MD = 10.98, 95% CI(6.97,14.99), P < 0.00001]. Compared with other oral medicine SXN could improve the P > levels effectively [MD = 8.49, 95% CI(2.40, 14.58), P = 0.006].And the subgroups analysis shown that compared with ferrous-sulfate there were significant differences [MD = 17.4, 95% CI(15.06, 19.73), P < 0.000 01]and the result of ferrous-succinate had significant differences [MD = 5.34, 95% CI(2.12, 8.56), P =0.001] too. Compared with Intravenous iron groups, there were statistical differences [MD =- 5.04, 95% CI(- 9.59,- 0.50), P = 0.03]. In the safety analysis, the rate of adverse reactions in SXN groups and control groups were 19.3% and 3.7%,respectively(P < 0.000 01). Due to our studies were of poor methodological quality, and the sample size were small, the results were influenced by bias.CONCLUSION: Our findings suggest that the SXN had better effect and was safer in the treatment of RA than ferrous succinate and ferrous sulfate.

Moxibustion Treatment for Knee Osteoarthritis: A Meta-Analysis

Objective:To systematically evaluate the efficacy of Moxibustion in the treatment of knee osteoarthritis, and to provide a reasonable supplementary scheme for clinical medical treatment by analyzing the results of Moxibustion in the treatment of knee osteoarthritis and related experimental studies.Methods:Computerized retrieval of CNKI, Wanfang, PubMed and other databases from January 2009 to June 2019, published clinical controlled trials of Moxibustion in the treatment of knee osteoarthritis, and retrospective references of the required literature to improve the relevant literature retrieval, if there are documents that can not be downloaded, Manual search was carried out and the literature was included after quality evaluation. Meta-analysis was carried out with Review Manager 5.3 software.Results:A total of 1207 patients with knee osteoarthritis were included in 15pieces of literature. (1) In terms of total effective rate, moxibustion was superior to conventional Western drugs. Meta-analysis showed that RR=1.21,95%CI [1.14,1.28],Z=6.44,P < 0.00001;(2) In VAS score, moxibustion was superior to conventional Western drugs, meta-analysis of two groups: WMD=-2.71, 95% CI [-4.90, -0.52], Z=2.43,P < 0.05;(3) In WOMAC score, moxibustion was superior to conventional Western drugs, meta-analysis of two groups: WMD=-6.79,95%CI [-12.35,-1.23],Z=2.39,P < 0.05;(4) WOMAC pain. In terms of pain score, moxibustion was superior to conventional Western drugs. Meta-analysis showed that WMD=-1.34,95%CI [-2.50,-0.17],Z=2.25,P < 0.05;(5) knee joint function. On the contrary, moxibustion was superior to conventional Western drugs. Meta-analysis showed that WMD = 12.54, 95% CI [6.93, 18.15], Z = 4.28,P < 0.01;(6) In Lysholm index, moxibustion was superior to conventional Western drugs. Meta-analysis showed that WMD = 12.13, 95% CI [6.87, 17.39], Z = 4.52,P < 0.01;(7) In Lequesne index, moxibustion was superior to conventional Western drugs. Routine western drugs, meta-analysis: WMD =-4.22, 95% CI [-5.74, -2.70], Z = 5.45,P < 0.01.Conclusions: The efficacy of Moxibustion in the treatment of knee osteoarthritis is better than that of conventional western drug therapy, but a large sample size and high quality randomized controlled trials are still needed to verify and analyze.