Changes in soil carbon stocks and related soil properties along a 50-year grassland-to-cropland conversion chronosequence in an agro-pastoral ecotone of Inner Mongolia,China

Land use change significantly influences soil properties. There is little information available on the long-term effects of post-reclamation from grassland to cropland on soil properties. We compared soil carbon （C） and nitrogen （N） storage and related soil properties in a 50-year cultivation chronosequence of grassland in the agro-pastoral ecotone of Inner Mongolia. Field surveys on land use changes during the period of 1955-2002 were conducted to build a chronosequence of cropland of different ages since the conversion from grassland. The results showed that soil C and N storage, soil texture, and soil nutrient contents varied with land use types and cropland ages （P〈0.01）. In the 0-30 cm soil layer, the soil organic carbon （SOC） density was significantly lower in the crop- lands （3.28 kg C/m2 for C50 soil） than in the grasslands （6.32 kg C/m2）. After 5, 10, 15, 20, 35, and 50 years of crop planting （years since the onset of cultivation）, the SOC losses were 17%, 12%, 19%, 47%, 46%, and 48%, respec- tively, compared with the grasslands. The soil total nitrogen （TN） density of the grasslands was 65 g N/m2, and TN density of the cropland soil was 35 g N/m2 after 50 years of crop planting. Both the SOC and TN densities could be quantitatively determined by a negative exponential function of cropland age （P〈0.0001, R2=0.8528; P〈0.0001, R2=0.9637）. The dissolved organic carbon （DOC） content, pH value were decreased; and the soil bulk density and soil available potassium （AK） content, clay content, and sand content were increased since the conversion of grassland into cropland during the 50-year period. Our results show soil nutrients were higher in grassland than in cropland. The conversion of grasslands to croplands induced a loss of soil C storage and changes of related soil properties. The reclamation time of cultivated soil （cropland age） had significant effects on soil properties in the study area.

Ecological stoichiometry and biomass response of Agropyron michnoi Roshev.under simulated N deposition in a sandy grassland,China

Sandy grassland in northern China is a fragile ecosystem with poor soil fertility.Exploring how plant species regulate growth and nutrient absorption under the background of nitrogen(N)deposition is crucial for the management of the sandy grassland ecosystem.We carried out a field experiment with six N levels in the Hulunbuir Sandy Land of China from 2014 to 2016 and explored the Agropyron michnoi Roshev.responses of both aboveground and belowground biomasses and carbon(C),N and phosphorus(P)concentrations in the plant tissues and soil.With increasing N addition,both aboveground and belowground biomasses and C,N and P concentrations in the plant tissues increased and exhibited a single-peak curve.C:N and C:P ratios of the plant tissues first decreased but then increased,while the trend for N:P ratio was opposite.The peak values of aboveground biomass,belowground biomass and C concentration in the plant tissues occurred at the level of 20 g N/(m2•a),while those of N and P concentrations in the plant tissues occurred at the level of 15 g N/(m2•a).The maximum growth percentages of aboveground and belowground biomasses were 324.2%and 75.9%,respectively,and the root to shoot ratio(RSR)decreased with the addition of N.N and P concentrations in the plant tissues were ranked in the order of leaves>roots>stems,while C concentration was ranked as roots>leaves>stems.The increase in N concentration in the plant tissues was the largest(from 34%to 162%),followed by the increase in P(from 10%to 33%)and C(from 8%to 24%)concentrations.The aboveground biomass was positively and linearly correlated with leaf C,N and P,and soil C and N concentrations,while the belowground biomass was positively and linearly correlated with leaf N and soil C concentrations.These results showed that the accumulation of N and P in the leaves caused the increase in the aboveground biomass,while the accumulation of leaf N resulted in the increase in the belowground biomass.N deposition can alter the allocation of C,N and P stoichiometry in the plant tissues and has a high potential for increasing plant biomass,which is conducive to the restoration of sandy grassland.

Responses of Phytolith in Guinea Grass(Leymus chinensis) Leaves to Simulated Warming,Nitrogen Deposition and Elevated CO_2 Concentration in Songnen Grassland,Northeast China

Deposited in plant cells and their intercellular space,phytoliths,a special form of silica,could be used to determine information on plant structure and physiology especially their size and content.With the hypothesis that phytolith in plant would change under variable climate and environment,the dominant plant species in Songnen grassland,guinea grass(Leymus chinensis),was treated by an open-top chamber(OTC) to elevate CO2 concentration,infrared heaters,and artificial nitrogen(N) addition for three years from 2006–2008.Phytoliths were extracted by wet-ashing method and analyzed by variance analysis and so on.We found that the responses to elevated CO2 are complicated,and warming is positive while N addition is negative to the deposition of phytoliths in L.chinensis leaves.Especially,warming could reduce the negative impact of N addition on phytolith in L.chinensis.The short cell's taxonomic in graminea is significant because of no disappearance with simulated environmental changes.The phytolith originated in the long cell and plant intercellular space are more sensitive to elevated CO2 concentration,warming,and N addition,and could become some new indicators for environmental changes.In conclusion,different phytolith types have various responses to simulated warming,N addition and elevated CO2 concentration.

Estimation and Spatio-temporal Patterns of Carbon Emissions from Grassland Fires in Inner Mongolia,China

Grassland fires results in carbon emissions,which directly affects the carbon cycle of ecosystems and the carbon balance.The grassland area of Inner Mongolia accounts for 22%of the total grassland area in China,and many fires occur in the area every year.However,there are few models for estimation of carbon emissions from grassland fires.Accurate estimation of direct carbon emissions from grassland fires is critical to quantifying the contribution of grassland fires to the regional balance of atmospheric carbon.In this study,the regression equations for aboveground biomass(AGB)of grassland in growing season and MODIS NDVI(Normalized Difference Vegetation Index)were established through field experiments,then AGB during Nov.–Apr.were retrieved based on that in Oct.and decline rate,finally surface fuel load was obtained for whole year.Based on controlled combustion experiments of different grassland types in Inner Mongolia,the carbon emission rate of grassland fires for each grassland type were determined,then carbon emission was estimated using proposed method and carbon emission rate.Results revealed that annual average surface fuel load of grasslands in Inner Mongolia during 2000–2016 was approximately 1.1978×1012 kg.The total area of grassland which was burned in the Inner Mongolia region over the 17-year period was 5298.75 km2,with the annual average area of 311.69 km2.The spatial distribution of grassland surface fuel loads is characterized by decreasing from northeast to southwest in Inner Mongolia.The total carbon emissions from grassland fires amounted to 2.24×107 kg with an annual average of 1.32×106 for the study area.The areas with most carbon emissions were mainly concentrated in Old Barag Banner and New Barag Right Banner and on the right side of the Oroqin Autonomous Banner.The spatial characteristics of carbon emission depend on the location of grassland fire,mainly in the northeast of Inner Mongolia include Hulunbuir City,Hinggan League,Xilin Gol League and Ulanqab City.The area and spatial location of grassland fires can directly affect the total amount and spatial distribution of carbon emissions.This study provides a reference for estimating carbon emissions from steppe fires.The model and framework for estimation of carbon emissions from grassland fires established can provide a reference value for estimation of carbon emissions from grassland fires in other regions.

Effects of Grassland Caterpillars on Plant Community Structure of Alpine Kobresia Meadows and Soil Properties

[Objective] To investigate the relationship between the grassland caterpillar with different grades and the structure of alpine Kobresia meadow or soil feature. [Method] A total of 10 plots （20.00 m × 20.00 m） were chosen. In each plot, five smaller plots （5.00 m × 5.00 m） were randomly selected and six sample plots （0.25 m × 0.25 m） were then selected in each smaller plot. The biomass, vegetation height, grasslayer thickness, bare land area, soil moisture and total vegetation cover degree were determined. Data were analyzed statistically by Excel 2003 and SPSS 13.0 software. [ Result] There was a significant difference （P 〈 0.05） between the grassland caterpillars at different hazard grades and the structure of alpine Kobresia meadow or soil properties. [ Conclusion] With the increase of grassland caterpillar density, the plant community structure of alpine Kobresia meadows changes from sedge family-dominant community to the forbs-dominant community.

Sensitivity of livelihood strategy to livestock production and marketization:An empirical analysis of grasslands in Inner Mongolia,China

Recent researches have primarily focused on the relationship between livelihood strategies and livelihood capital,with few empirical studies on the sensitivity of livelihood strategies to livestock production and marketization in Inner Mongolia Autonomous Region of China.This study used an income distribution approach to categorize livelihood strategies of the respondents(n=394)into three types,i.e.,herder livelihood strategy(LS1),petty-herder livelihood strategy(LS2),and non-herder livelihood strategy(LS3).Using the multinomial logistic regression model,we compared livestock production and marketization across the three livelihood strategies.Our findings showed that(1)livestock production and marketization tended to favor LS1;(2)an increase in the land asset(contracted and rented grassland)and off-take rate increased the probability of households choosing LS1;(3)stocking rate was higher for LS1;and(4)the higher critical market-related risks perceived by herders were animal price and hay and corn price.Moreover,higher livestock price acted as a deterrent to diversifying into other livelihood strategies(LS2 and LS3).Finally,this study advocates for policies that will promote the land transfer market,adopt modern techniques in animal husbandry,improve the medium for disseminating market information to herders,and provide incentives for long-term livelihood transformation.

Effect of Caragana korshinskii Silage at Different Growth Stages on the Feeding of Livestock

[Objectives] Caragana korshinskii resources are rich in Ordos with a large planting area, but they have not been fully and reasonably utilized. This study aims to effectively convert C. korshinskii into high-quality feed, improve the quality of milk and meat of herbivores and increase production. [Methods] The utilization of C. korshinskii in feed was studied in various aspects, the factors were selected, the level was determined, and the multi-factor experiment—orthogonal experiment was conducted. [Results] The effect on the growth of sheep was in the order of silage > C. korshinskii feed > corn stalk silage > hay meal, and the effect on the growth of goats was in the order of hay meal > C. korshinskii feed > silage > corn stalk silage. [Conclusions] From the analysis of the range results, the best level combination was selected: in different growing seasons, C. korshinskii branch and leaf silage in full-bloom stage had the greatest effect on the growth of sheep and goats, and the fattening effect was the best;among the different silage methods of C. korshinskii, the C. korshinskii—corn stalk mixture silage had a good feeding effect for sheep and goats.

Responses of Plant Community and Soil Properties to Inter-Annual Precipitation Variability and Grazing Durations in a Desert Steppe in Inner Mongolia

Grazing can dramatically affect arid grassland communities that are very vulnerable to environmental changes due to its relatively short and sparse ground coverage, low biomass, sandy soil and inter-annual precipitation found in the desert steppe. The study investigates the effects of different grazing durations on vegetation and soil properties of a desert steppe community. The experiment was conducted in Xisu Banner in Inner Mongolia with ifve treatments：CG （continuous grazing）, 40UG （40 d ungrazed）, 50UG （50 d ungrazed）, 60UG （60 d ungrazed） and UG （ungrazed）. The biomass of both shrub and annual-biennial plant communities were signiifcantly decreased by CG. Continuous grazing and 40UG signiifcantly reduced the ANPP （aboveground net primary productivity） by the end of the three year study. 60UG treatment increased soil organic carbon （OC）, total nitrogen concentration （TN） and total phosphorus concentration （TP） concentrations and 50UG increased the TN and total phosphorus concentration （TK） concentrations, whereas CG, 40UG and 50UG decreased soil OC, TP and available phosphorus concentration （AP） concentrations. The perennial plant species of the desert steppe were generally tolerant for grazing. The annual-biennial plant species had large variability in ANPP because of the inter-annual precipitation. Our results highlight that inter-annual precipitation variations could strongly modify the community responses to grazing in arid ecosystems.

Vegetation Community Convergence of Pastoralists' Pasture at Different Economic Levels in Desert Steppe

Vegetation characteristics of pastoralist households’ pastures at different economic levels were quantiifed to compare the grassland conditions in Sunite Right Banner, Inner Mongolia, China in 2011 and 2012. The results showed that the heights of Stipa klemenzis and Cleistogenes songorica were similar among economic treatments. And the height of Allium polyrhizm had no signiifcant differences between high-economic-level （HEL） and low-economic-level （LEL） treatments. There were no signiifcant difference among treatments in the canopy cover of C. songorica. The densities of dominant species （S. klemenzis, C. songorica and A. polyrhizm） were signiifcantly similar among treatments. Aboveground community biomass in reference area （CK） was higher than that in other three treatments in 2011, but their biomass showed no signiifcant difference among three economic treatments;for 2012, biomass in HEL was higher than low-economic-level treatment （LEL）, while the biomass in middle-economic-level treatment （MEL） and CK was similar to that in HEL and LEL, respectively. Aboveground biomass of S. klemenzis and C. songorica was similar among treatments in both 2011 and 2012. Biomass of A. polyrhizum had no signiifcant difference between HEL and MEL. Households of LEL had rented out excess lands and those of HEL had leased their available lands thus resulting uniform utilization and convergence of pastoralist households’ pastures across all economic levels.

Spatial Effect of Mineral Resources Exploitation on Urbanization:A Case Study of Tarim River Basin,Xinjiang,China

Mineral resources exploitation significantly affects the spatial structure and evolutive trend of urbanization in arid areas.In this study,the spatial autocorrelation method and the spatial computation model were used to investigate the spatial impacts of mineral resources excavation and processing on comprehensive level of urbanization in the Tarim River Basin,Xinjiang,China for the years of 2000,2006 and 2008.The results are as follows:1)There was a spatial correlation of the development of mineral resources industry and the comprehensive level of urbanization in 2006 and 2008,with the spatial concentration trend rising significantly in 2006 and decreasing in 2008.2)The patterns of local spatial concentration of mineral resources industry and of the comprehensive level of urbanization were relatively stable,and the two patterns showed signs of spatial correlationship.The higher was the degree of the concentration of mineral resources industry,the stronger was its impact on the spatial clustering of urbanization.3)In 2000, mineral resources excavation and processing had a significant positive impact on the comprehensive level of urbanization in the region but not in its adjacent regions.However,in 2008,mineral resources excavation and processing significantly raised the comprehensive level of urbanization not only in the region but also in its neighboring regions.This research demonstrates that the development of mineral resources industry can strongly affect the trends and spatial patterns of urbanization.

VOR and CVOR Index for Health Evaluation of Typical Steppe in Inner Mongolia

[Objective] The aim of this study was to evaluate the effect of livestock exclusion on the health of grassland ecosystem accurately and quantitatively. [Method] One open grazing site and five seasonal exclusion sites, which had the same vegetation composition （taking Leymus chinensis and Stipa krylovii as main species） and soil type, were chosen as research objects for the VOR and CVOR evaluation of the effect of open grazing and livestock exclusion on the health of typical steppes, and for building the calculation model for VOR and CVOR indices to evaluate the health of typical steppes with different exclusion periods. [ Result] The VOR and CVOR composite indices both could accurately evaluate the health of typical steppes, and the evaluation results from the both indices were quite consistent. Although the condition （C）, vigor（V）, organization （O） and resilience（ R ） indices changed differently in different exclusion periods, the VOR and CVOR composite indices increased gradually with the peri- od of exclusion prolonged, and the health condition of typical steppe changed from ＂alert＂ level （in the open grazing and earlier exclusion period） to ＂healthy＂ level （after 13 to 20 years＇exclusion）. [ Conclusion] The application of exclusion was beneficial to the recovery of degraded grassland ecosystem.

Progress in Relationship between Anatomical Structures of Vegetative Organs and Drought Resistance in Forage Germplasm Resources

The anatomical structures of roots, stems and leaves are very important indicators to＇evaluate drought resistance. The paper reports the relationship between anatomical structures of vegetative organs and drought resistance in forages.

Impact of Climate and Grazing on Biomass Components of Eastern Russia Typical Steppe

Spatial and structural characteristics of plant communities in the steppe ecosystems of the Baikal region, Russian Federation have been researched in connection with climate change and grazing. The present study, based on a total of 15 typical steppe sampling plots, investigated above-ground biomass （AGB）, below-ground biomass （BGB）, total biomass （TB） and root：shoot ratios （R：S） and their relationships with climatic factors and grazing. All biomass components varied widely depending on the climatic parameters and the degree of grazing affected transformation. A strong negative correlation between mean annual temperature （MAT） and total plant biomass was revealed for all study area. Mean annual precipitation （MAP） signiifcantly affected communities productivity increasing only in the south of the region. Due to the large and mountainous territory, the inlfuence of latitude and elevation （mountain） factors on the components of the biomass were studied. Although all studied plant steppe communities were transformed by uncontrolled grazing, their productivity is signiifcantly reduced only at plots with maximum digression. Vegetation shift is an indicator of climate change, as well as providing a diagnostic tool to build predictive models. Based on the complex index of effective precipitation, it was revealed that at the end of last century in the steppes of the Baikal region the structural and production processes will be affected by an arid climate trend.

Effects of water and nitrogen addition on vegetation carbon pools in a semi-arid temperate steppe

Global change will lead to increases in regional precipitation and nitrogen（N） deposition in the semi-arid grasslands of northern China. We investigated the responses of vegetation carbon（C） pools to simulated precipitation and N deposition increases through field experiments in a typical steppe in Inner Mongolia. The treatments included NH4NO3 addition at concentrations of 0（CK）, 5（LN, low nitrogen）, 10（middle nitrogen, MN）, and 20（HN,high nitrogen）（g m^（-2）a^（-1）） with and without water. After three consecutive years of treatment, from 2010 to 2012,water addition did not significantly change the size of the total vegetation C pools, but it significantly decreased the ratio of root：shoot（R：S）（P = 0.05） relative to controls. By contrast, N addition significantly increased the total vegetation C pools. The C pools in the LN, MN and HN treatments increased by 22, 39 and 44 %, respectively. MN produced the largest effect among the N concentrations,although differences between N-added treatments were not significant（P ？ 0.05）. N addition significantly reduced the ratio of root：shoot（R：S）（P = 0.03）. However, there were no significant interactive effects of water and N addition on the vegetation C pools.

Effects of Artificial Aging on Physiological Characteristics and Seed Vigor of Medicago ruthenica Seeds

As shown by the changes in physiological characteristics and seed vigor of three Medicago ruthenica samples during artificial aging process, the germination potential, germination rate, germination index, vigor index and simple vigor index declined with the extension of aging time on the whole. After artificial aging for 6 min, the vigor was higher than that of the control. The conductivity, malondialdehyde content and soluble polysaccharide content of seed leachate increased with the aging time and negatively correlated （ P 〈 0.05） with the seed vigor indicators. The physiological indicators were very significantly correlated （P 〈 0.01 ） with the vigor indicators in the Medicago ruthenica sample from Dorbod Qi, UIanqab City（China）.

Thermal-physiological Strategies Underlying the Sympatric Occurrence of Three Desert Lizard Species

Sympatric reptiles are the ideal system for investigating temperature-driven coexistence. Understanding thermally physiological responses of sympatric lizards is necessary to reveal the physiological mechanisms that underpin the sympatric occurrence of reptiles. In this study, we used three lizard species, Eremias argus, E. multiocellata, and Phrynocephalus przewalskii, which are sympatric in the Inner Mongolia desert steppe, as a study system. By comparing their resting metabolic rates(RMR) and locomotion at different body temperatures, we aimed to better understand their physiological responses to thermal environments, which may explain the sympatric occurrence of these lizards. Our results showed that E. argus had significantly higher RMR and sprint speed than E. multiocellata, and higher RMR than P. przewalskii. In addition, the optimal temperature that maximized metabolic rates and locomotion for E. argus and E. multiocellata was 36°C, whereas for P. przewalskii it was 39°C. Our study revealed the physiological responses to temperatures that justify the sympatric occurrence of these lizards with different thermal and microhabitat preferences and active body temperatures. Eremias argus and E. multiocellata, which have lower body temperatures than P. przewalskii, depend on higher RMR and locomotion to compensate for their lower body temperatures in field conditions. Our study also highlights the importance of using an integrative approach, combining behavior and physiology, to explore the basis of sympatric occurrence in ectothermic species.

Biodegradation of Lubricating Oil in Wastewater with Zoogloea sp.

The purpose of this study was to identify microbial strains that have a strong ability to biodegrade lubricating oil. No. 20 lubricating oil was used as the sole carbon source in an isolation medium to screen bacteria from sludge that had been contaminated with crude oil. Through both morphological and biochemical methods, the bacterial strain that had the highest biodegrading capacity was identified as Zoogloea sp. On the basis of these preliminary results, a biological contact oxidation method was employed to further assess the ability of the Zoogloea sp. strain to treat wastewater contaminated by No. 20 lubricating oil using three oxidation cabins and two hydraulic retaining times （HRT）. Results showed that the concentration of No. 20 lubricating oil with a 12 h hydraulic retaining time and 16.5 L h^-1 inflow rate was reduced by 99.3% in 15 d, and with a 6 h hydraulic retaining time and 33 L h%^-1 inflow rate it decreased by 98.6070 in 12 d. The results from this study indicated that Zoogloea sp. had a strong potential to be utilized in biodegradation of lubricating oil.

Impact of Climate Change and Grazing on Temperate Steppe

Ecosystem of eastern Eurasian steppe is facing more and more challenges of global issues such as climate change, food and ecological security and human excessive utilization since the 21st century. Facing on the increasingly prominent international issues, it is very essential for relevant countries, international organizations and domestic counterparts to enhance systematic and mutual cooperation and exchanges to carry out scientific research and develop protection practice on the Eurasian steppe.

Study on Soil Respiration Characteristics and Carbon Balance of <i>Kobresia pygmaea</i>Meadow in Qinghai-Tibet Plateau, China

Although soil respiration is the largest contributor to C flux from terrestrial ecosystems to the atmosphere, our understanding of its characteristics and carbon budget in alpine meadow is rather limited because of extremely geographic situation. This study was designed to examine soil CO2 efflux characteristics of diurnal and seasonal variation, thus obtaining estimates of carbon balance of Kobresia pygmaea meadow in Qinghai-Tibet plateau. The results showed that the soil respiration of diurnal and seasonal rate changed little in growing season and was mainly affected by temperature, and single peak curve that showed afternoon appeared. Composite model which was set by soil respiration rate, soil moisture content and temperature (atmospheric temperature and soil temperature) could explain better the variations of soil respiration rate. The variation range of Q10 ranged from 1.28 to 2.34, which was sensitive to temperature in green-up period and late growth stage, and decreased in growth peak period. Meanwhile, during the growing seasons the observed amount of annual carbon fixation via primary production for Kobresia pygmaea meadow ecosystem was about 120.21 g C·m-2·a-1. The carbon dioxide output via soil heterotrophic respiration was about 37.54 g C·m-2·a-1. So carbon budget had more input than output. The Kobresia pygmaea meadow ecosystem has stronger potential to absorb carbon dioxide, it was a sink of atmospheric CO2, and the plant community had a net carbon gain of 82.67 g C·m-2·a-1.

Environmental Monitoring in Northern Aksu, China Based on Remote Sensing Ecological Index Model

In order to understand the development status of ecological environment quality in the Aksu region of China, to effectively adjust the ecological environment quality, so as to promote the sustainable development of its social economy and ecological environment protection. This paper selects the Landsat series remote sensing images of the northern Aksu region in 2013, 2016, and 2019, and uses the tools such as ENVI5.3 and ArcGIS 10.8.1 to process the image data accordingly. The principal component analysis method is used to calculate the Remote Sensing Ecological Index (RSEI) of the northern Aksu region. The data show that: 1) The ecological environment quality index in the northern Aksu region in 2013, 2016, and 2019 was 0.706087, 0.25243 and 0.362991 respectively;2) The areas where the ecological environment quality declined significantly in the northern Aksu region were the human settlements and the Gobi, fan-shaped land and other special terrain areas;3) The humidity index and the heat index are the two factors that have the greatest impact on the ecological environment quality in the northern Aksu area. The data as a whole show that the ecological environment in the northern part of the Aksu region has deteriorated seriously, and the severely deteriorated area is close to the human living area.