The intestinal epithelium is responsible for food digestion and nutrient absorption and plays a critical role in hormone secretion,microorganism defense,and immune response.These functions depend on the integral singl...The intestinal epithelium is responsible for food digestion and nutrient absorption and plays a critical role in hormone secretion,microorganism defense,and immune response.These functions depend on the integral single-layered intestinal epithelium,which shows diversified cell constitution and rapid self-renewal and presents powerful regen-eration plasticity after injury.Derailment of homeostasis of the intestine epithelium leads to the development of dis-eases,most commonly including enteritis and colorectal cancer.Therefore,it is important to understand the cellular characterization of the intestinal epithelium at the molecular level and the mechanisms underlying its homeostatic maintenance.Single-cell technologies allow us to gain molecular insights at the single-cell level.In this review,we summarize the single-cell RNA sequencing applications to understand intestinal cell characteristics,spatiotemporal evolution,and intestinal disease development.展开更多
基金This work was supported by grants from the National Natural Science Foundation of China(31988101 and 31900550)the Guangdong Postdoctoral Research Foundation(O0390302)+1 种基金Guangdong Basic and Applied Basic Research Foundation(2021A1515111215)China Postdoctoral Science Foundation(2021M703230 and 2022T150653).
文摘The intestinal epithelium is responsible for food digestion and nutrient absorption and plays a critical role in hormone secretion,microorganism defense,and immune response.These functions depend on the integral single-layered intestinal epithelium,which shows diversified cell constitution and rapid self-renewal and presents powerful regen-eration plasticity after injury.Derailment of homeostasis of the intestine epithelium leads to the development of dis-eases,most commonly including enteritis and colorectal cancer.Therefore,it is important to understand the cellular characterization of the intestinal epithelium at the molecular level and the mechanisms underlying its homeostatic maintenance.Single-cell technologies allow us to gain molecular insights at the single-cell level.In this review,we summarize the single-cell RNA sequencing applications to understand intestinal cell characteristics,spatiotemporal evolution,and intestinal disease development.
