Clinical Observation on Treatment of 78 Patients of B-Thalassemla with Bushen Ylsul (补肾益髓) Method and Study on Its Molecular Mechanism

Objective: To study the treatment of B-Thalas-semia (ThE) with Chinese herbal medicine for Bushen Yisui (BSYS), its theoretical base and molecular mechanism. Methods: Seventy-eight patients with ThE were treated with BSYS recipe (consisted of 11 Chinese herbal drugs as Dogwood fruit, Fleeceflower root, prepared Rehmannia root and turtle shell, etc.) orally taken, 3 times per day, 10 g/time, 3 months as one therapeutic course. Hemoglobin (Hb), red blood cell (RBC), reticulocyte (Ret) and hemoglobin F (HbF) were checked every month. At the same time, PAGE, PVR, PCR-SSCP, RT-PCR, DNA series analysis, mRNA gene expression analysis techniques were used to conduct the systematic gene analysis in patients to study the molecular mechanism of TCM treatment from aspects of gene mutation, gene expression and control-regulation. Results: All the blood criteria in patients after BSYS treatment were improved significantly with clinical symptoms

EFFECT OF PROCYANIDIN ON CELL CYCLE IN MURINE LEWIS LUNG CARCINOMA

Objective: To investigate the anti-tumor effect of procyanidin (WeiMaiNing, WMN) on a murine Lewis lung carcinoma cell line (3LL) and the influence on the cell cycle. Methods: The inhibitory rate of 3LL growth was detected in the model of murine Lewis lung carcinoma. The effect of the drug on 3LL cell cycle and the influence of the drug on the expression of cyclin D1 protein were investigated by flow cytometry, immunohistochemical staining. Results: The inhibitory rates of WMN in 3LL were 19.14%, 33.59% and 51.56% respectively at dosages 100 mg穔g-1穌-1, 150 mg穔g-1穌-1 and 250 mg穔g-1穌-1. The inhibitory effect was in a dose-dependent manner. We found WMN significantly increased (P<0.01) the number of 3LL cells in G0-G1 phase (35.97% vs. 27.2% at 150 mg穔g-1穌-1 WMN; 40.10% vs. 27.2% at 250 mg穔g-1穌-1 WMN) and decreased the expression of cyclin D1, PCNA protein. Conclusion: WeiMaiNing inhibits the growth of 3LL cells in vivo by decreasing the expression of PCNA and cyclin D1, blocking the cells in G0-G1 phase and preventing the cells transition from G1 to S phase while DNA is replicated.

Establishment of TCM Standardized Therapeutic Regimen for Treating Tumor

It has been of a long long history to treat tumor with traditional Chinese medicine (TCM), in the last 50 years, Chinese medicine (CM) has been accepted by more and more medical professionals and patients, and used as one of the principal methods

Clinical correlation between myeloperoxidase and acute coronary syndrome

Objective To study whether myeloperoxidase (MPO) can provide prognostic information in patients with acute coronary syndromes (ACS). Methods The study population consisted of 274 consecutive patients with ACS. All patients underwent coronary angiography which showed significant coronary artery disease and blood samples were collected at admission. Follow-ups were scheduled at 1, 3, and 6 months.The end point included cardiac death, acute myocardial infarction (MI), percutaneous or surgical revascularization. Results Patients with elevated MPO serum levels (MPO ≥ 72.2 AUU/L) were more likely to have diabetics and had a history of coronary events. Kaplan-Meier event rate curves with accumulative incidence of end point at 6-month follow-up in the MPO ≥ 72.2 AUU/L group was significantly higher than in MPO<72.2 AUU/L group. Conclusions MPO may be a powerful predictor of adverse outcome in patients with ACS.(J Geriatr Cardiol 2007;4:209-212)

Correlation Analysis between TCM Syndromes and Physicochemical Parameters

Traditionally, differentiation of syndromes of Traditional Chinese Medicine (TCM) mainly depends on the information obtained from four diagnosis methods. Now many physicochemical parameters are available in clinic. There exists great correlation between TCM syndromes and physicochemical parameters. The objective of the paper is to analyze the correlation between TCM syndromes and physicochemical parameters quantitatively. Correlation analysis has been widely studied and many analysis methods have been developed. Mutual information based on entropy can measure arbitrary dependence between variables. It has been applied to many kinds of fields, especially to pattern recognition. But most works are restricted to discrete variables and little work has been done to study the relation between discrete and continuous variables. A novel algorithm is proposed to calculate the mutual information between discrete and continuous variables. It is used to analyze the correlation between TCM syndromes and physicochemical parameters.

Clinical Assessment on Treatment of Hyperlipidemia with Pushen Capsule (蒲参胶囊)

Objective: To explore the therapeutic effect of Pushen capsule (PSC, 蒲参胶囊) in treating primary hyperlipidemia. Methods: Two hundred and forty patients with primary hyperlipidemia were randomly divided into two groups, the 120 patients in the treated group treated with PSC (4 capsules, tid) and the 120 patients in the control group treated with Zhibituo tablet (脂必妥, 3 tablets, tid), and they were administered at the same time with Zhibituo placebo. The therapeutic course for both groups was 4 weeks. The therapeutic effect and the effects on blood lipids and viscosity were observed. Results: The effective rate in thetreated group was 76. 3%, which was significantly higher than that in the control group (48. 7% , P< 0. 01). PSC showed a significant lowering effect on TC, TG and LDL-C and raising effect on HDL-C, and the effect in lowering TG was significantly better than that of Zhibituo (P<0.01). PSC also showed a certain effect in decreasing whole blood viscosity of both high-sheared and low-sheared viscosity. Conclusion: Pushen capsule has promising blood lipid regulating effect in patients with hyperlipidemia, and some effects in lowering the blood viscosity.Original article on CJITWM (Chin) 2004 ;24 (3): 227

Analysis of Chinese Medical Syndrome Features of Ischemic Stroke Based on Similarity of Symptoms Subgroup

Objective: To derive the Chinese medicine(CM) syndrome classification and subgroup syndrome characteristics of ischemic stroke patients. Methods: By extracting the CM clinical electronic medical records(EMRs) of 7,170 hospitalized patients with ischemic stroke from 2016 to 2018 at Weifang Hospital of Traditional Chinese Medicine, Shandong Province, China, a patient similarity network(PSN) was constructed based on the symptomatic phenotype of the patients. Thereafter the efficient community detection method BGLL was used to identify subgroups of patients. Finally, subgroups with a large number of cases were selected to analyze the specific manifestations of clinical symptoms and CM syndromes in each subgroup. Results: Seven main subgroups of patients with specific symptom characteristics were identified, including M3, M2, M1, M5, M0, M29and M4. M3 and M0 subgroups had prominent posterior circulatory symptoms, while M3 was associated with autonomic disorders, and M4 manifested as anxiety;M2 and M4 had motor and motor coordination disorders;M1 had sensory disorders;M5 had more obvious lung infections;M29 had a disorder of consciousness. The specificity of CM syndromes of each subgroup was as follows. M3, M2, M1, M0, M29 and M4 all had the same syndrome as wind phlegm pattern;M3 and M0 both showed hyperactivity of Gan(Liver) yang pattern;M2 and M29 had similar syndromes, which corresponded to intertwined phlegm and blood stasis pattern and phlegm-stasis obstructing meridians pattern, respectively. The manifestations of CM syndromes often appeared in a combination of 2 or more syndrome elements. The most common combination of these 7 subgroups was wind-phlegm. The 7 subgroups of CM syndrome elements were specifically manifested as pathogenic wind, pathogenic phlegm,and deficiency pathogens. Conclusions: There were 7 main symptom similarity-based subgroups in ischemic stroke patients, and their specific characteristics were obvious. The main syndromes were wind phlegm pattern and hyperactivity of Gan yang pattern.

Efficacy evaluation of summer acupoint application treatment on asthma patients: a two-year follow-up clinical study

OBJECTIVE: To evaluate the self-efficacy and satisfaction of asthma patients subjected to summer acupoint application treatment(SAAT).METHODS: A two-year follow-up clinical study was conducted. Patients with asthma were treated by applying a herbal paste onto the Feishu(BL 13) and Fengmen(BL 12) acupoints on the three hottest days of summer, according to the traditional Chinese calendar, from 2008 to 2010. During a two-year follow-up, these patients were asked tofill out a questionnaire evaluating asthma degree,exacerbation frequency, concomitant medications and self-satisfaction. The self-rate and doctor-report outcomes obtained in parallel were evaluated to assess the efficacy of SAAT.RESULTS: A total of 527 asthma patients were initially enrolled in this study, of which 97 elderly patients and those with more severe cases of asthma were lost to follow-up. Thus, a total of 430 patients were valid for analysis using self-rate data. Nevertheless, occasional negative returns were obtained;almost all of the outcomes were rated as "No change", "Moderate effective", or "Very effective". In addition, 80% of the patients were satisfied with this treatment. Moreover, 391(91.4%) patients were somewhat improved after SAAT in 2009, and further improvement was observed in 2010. After SAAT, the average asthma-degree score decreased from 5.3 in 2008 to 4 in 2009 and, subsequently to3.5 in 2010.CONCLUSION: With pronounced patient satisfaction, SAAT can reduce the exacerbation severity and frequency, concomitant medications and asthma degree. Prolonging the treatment course might enhance the efficacy of SAAT.

Distribution and Development of the TCM Syndromes in Psoriasis Vulgaris

Objective: To study the distribution and development rules of the TCM syndromes in psoriasis vulgaris. Methods: Based on the clinical epidemiologic mass survey, the study was carried out by means of a status survey, multi-center and large-sample research. The data base was set up by EPIINFO6.0. The SPSS was used to do the statistical analyses in 2651 cases of psoriasis vulgaris to study the correlations among the distribution and development of the TCM syndromes, the stages of the disease, nationality, psoriasis history, family history, smoking history, alcohol drinking history, and severity of the disease. Results: The TCM syndromes in psoriasis vulgaris mainly include the blood-heat syndrome (53.8%), blood-dryness syndrome (27.4%), and blood-stasis syndrome (18.1%). Other syndromes were rarely seen, covering 0.6%. The concurrent syndromes mainly involve dampness, heat, blood stasis and toxin. The distribution differences of the main syndromes at different stages of the disease had statistical significance (P<0.01). The syndrome distribution is not related with nationality and family history (P>0.05), but it was closely related with the psoriasis history, smoking history, alcohol drinking history, and severity of the disease (P<0.01). Conclusion: At the initial stage, psoriasis vulgaris usually manifests itself as the blood-heat syndrome, and later it may be improved or turn into the blood-dryness or blood-stasis syndrome. Smoking, alcohol consumption, and severity of the disease may play a role in the syndrome's transformation.

Thirty-two Cases of Vascular Headache Treated by Acupuncture Combined with Chinese Herbal Decoction

Objective:To compare the acupuncture plus oral administration of Chinese herbal decoction with simple oral administration of Chinese herbal decoction in the treatment of vascular headache.Methods:Sixty two patients were randomly divided into a treatment group (32 cases) and a control group (30 cases).Acupuncture at Baihui (GV 20),Fengchi (GB 20),Shuaigu (GB 8),Xingjian (LR 2),Neiguan (PC 6),Sanyinjiao (SP 6) and Ashi points combined with oral administration of Chinese herbal decoction,was applied in the treatment group,and simple oral administration of Chinese herbal decoction was applied in the control group.Results:The total therapeutic effect in the treatment group was better than that in the control group (P<0.05).After treatment,the frequency,and duration of the attacks were reduced and shortened,and headache greatly alleviated in both groups (P<0.01).The alleviation in the treatment group was more obvious than that in the control group (P<0.05).Conclusion:Acupuncture combined with oral administration of Chinese herbal decoction provided remarkable therapeutic effects in treating vascular headache.

Effect of moxa smoke produced during combustion of Aiye(Folium Artemisiae Argyi) on behavioral changes in mice inhaling the smoke

OBJECTIVE: To evaluate the effects that inhalation of the combustion products of the traditional Chinese herb Aiye(Folium Artemisiae Argyi) has on the central nervous system.METHODS: Forty Kunming mice(half male) were randomly assigned(n = 10/group) to a control group(C) or one of three moxa smoke concentration groups(% opacity): low(L1; 0.4%), medium(M1; 2%), and high(H1; 15%). Mice in the latter three groups were exposed to moxa smoke in a dynamic gas exposure cabinet for 20 min per day for7 days. Mice in control group were placed in the same cabinet without any intervention. For the sleep experiments, another 50 mice were divided into five groups of 10 mice each: a saline-injected control group, L1 + pentobarbital sodium(PS)-injected group, M1+PS group, H1(15%)+PS group,and a positive control group(10 mg/kg, chlorpromazine, p.o.). The weight, general activities, locomotor activities, rotarod performance, sleep duration,and sleeping rate induced by a subthreshold dose of pentobarbital sodium were recorded in the mice,and the composition of moxa smoke was analyzed using headspace gas chromatography(GC-HS).RESULTS: A low concentration of smoke significantly decreased the frequency of locomotor activities and the time for which the mice remaining on the rotarod; however, a high smoke concentration significantly prolonged the pentobarbital-induced sleeping time and sleeping rate.CONCLUSION: The concentration-dependent relaxing effects of moxa smoke on the Central Nervous System(CNS) were confirmed. Moreover, GC-HS analysis showed that the component present in the highest concentration in moxa smoke was eucalyp-tol, an essential oil well recognized for its soothing effects on the CNS. This may therefore be accountable for the sedative effects of moxa smoke.

Multistage analysis method for detection of effective herb prescription from clinical data

Determining effective traditional Chinese medicine （TCM） treatments for specific disease conditions or particular patient groups is a difficult issue that necessitates investigation because of the complicated personalized manifestations in real-world patients and the individualized combination therapies prescribed in clinical settings. In this study, a multistage analysis method that integrates propensity case matching, complex network analysis, and herb set enrichment analysis was proposed to identify effective herb prescriptions for particular diseases （e.g., insomnia）. First, propensity case matching was applied to match clinical cases. Then, core network extraction and herb set enrichment were combined to detect core effective herb prescriptions. Effectiveness-based mutual information was used to detect strong herb symptom relationships. This method was applied on a TCM clinical data set with 955 patients collected from well-designed observational studies. Results revealed that groups of herb prescriptions with higher effectiveness rates （76.9% vs. 42.8% for matched samples; 94.2% vs. 84.9% for all samples） compared with the original prescriptions were found. Particular patient groups with symptom manifestations were also identified to help investigate the indications of the effective herb prescriptions.

Evaluation of the Curative Effect of Electroacupuncture on Postapoplectic Urinary Incontinence

目的:评价电针治疗中风后尿失禁的疗效。方法:将153例患者随机分为治疗组102例、对照组51例,治疗组采用电针治疗,对照组采用西药治疗,治疗1～2月后观察疗效。结果:治疗组基本治愈率为47.0%,显效率为27.5%,有效率为18.6%,无效率为6.9%,总有效率为93.1%;对照组基本治愈率为7.9%,显效率为17.6%,有效率为17.6%,无效率为56.9%,总有效率为43.1%,两组疗效比较差异有统计学意义(P<0.05),治疗组疗效明显优于对照组。结论:电针治疗中风后尿失禁疗效好。

Acupuncture Treatment for 35 Cases of Urticaria

In TCM it is held that urticaria is mostly due to weak constitution with deficiency of qi and blood, or prolonged illness that causes consumption of qi and blood. Such constitution can result in formation of wind due to blood deficiency, and internal-external lack of resistance due to qi-deficiency, which

Jinye Baidu granule for wind-warmth lung-heat disease(heat in the lung-wei)： protocol for a randomized, double-blind, parallel, controlled trial

OBJECTIVE: To observe the curative effect of Jinye Baidu granule in the treatment of fever and swollen and sore throat caused by wind-warmth lung-heat disease(heat in the lung-wei) to further identify the indications.METHODS: This randomized, double-blind, parallel, controlled trial will include patients with acute upper respiratory infection and wind-warmth lung-heat disease(heat in the lung-wei). Patientswith serious bacterial infection(white blood cell count > 12 × 10~9, neutrophils > 80%) will be excluded. Patients will be divided into three categories(blocks) according to their condition: fever only, a swollen and sore throat, and combined fever plus a swollen and sore throat. Patients within each of the three blocks will be further divided into a treatment group and a control group via stratified blocked randomization. The treatment group will be treated with Jinye Baidu granule, and the control group will be treated with Fufang Shuanghua granule. The primary outcome measure will be body temperature recovery time for patients with fever, throat symptom score for patients with a swollen and sore throat, and body temperature recovery time and throat symptom score for patients with combined fever plus a swollen and sore throat. Routine blood testing, urine testing, liver function, kidney function and ECG data of all patients will be collected as safety indices before and after enrollment, and adverse events will be recorded during the whole trial course.CONCLUSION: This study protocol will include stratified block analysis according to patients' symptoms, and identify the accurate clinical indications of Jinye Baidu granule. It will also enable safety evaluation from laboratory indices and adverse events, which will provide reliable evidence for clinical treatment.

Establishment of an in vitro model using the rat alveolar macrophage cell line NR8383

OBJECTIVE:To establish an in vitro model of radiation-induced lung injury using rat lung alveolar macrophages(NR8383).METHODS:Using a medical electronic linear accelerator,cells were irradiated with either 0 Gy or 6 Gy X-rays.At 6,12,24,30 and 48 h,the DNA damage index(8-OHd G)and lipid damage index(MDA)were measured in the two groups.We also determined the levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6)and transforming growth factor-β(TGF-β).RESULTS:The levels of 8-OHd G and MDA in the 6 Gy irradiation group were higher than those in the0 Gy group at 6,12,24,30 and 48 h after irradiation.The levels reached the highest value-6 h after irradiation,and then gradually decreased.The levels of the inflammatory factors TNF-α,TGF-βand IL-6 were higher in the 6 Gy irradiation group than those in the 0 Gy group at 6,12,24,30 and 48 h after irradiation.CONCLUSION:Six Gy X-ray irradiated NR8383 cells can be used to establish an in-vitro model of radiation-induced lung injury.The levels of 8-OHd G,MDA,TNF-α,TGF-βand IL-6 can be used as effective evaluation indicators.

Understanding traditional Chinese medicine

Traditional Chinese Medicine(TCM),a collection of ancient Chinese knowledge and cultural wisdom,is a life science that has been passed down for thousands of years.Supporters of TCM stress its clinical efficacy,and while the particular characteristics and reliability of TCM have received widespread attention,they have also invited considerable criticism.This article aims to discuss some of these controversial topics.

Introduction to Quick and Surround Needling Method for Nodular Goiter

临床上以中医整体思想为指导,通过望、闻、问、切四诊合参,辨证论治,以快针围刺法为主,辅以体针,治疗结节性甲状腺肿,充分发挥了特殊针刺疗法的实际应用价值。