Real-time identification of multiple nanoclusters with a protein nanopore in single-cluster level

It is important and challenging to analyze nanocluster structure with atomic precision.Herein,α-hemolysin nanopore was used to identify nanoclusters at the single molecule level by providing two-dimensional(2D)dwell time–current blockage spectra and translocation event frequency which sensitively depended on their structures.Nanoclusters such as Anderson,Keggin,Dawson,and a few lacunary Dawson polyoxometalates with very similar structures,even with only a two-atom difference,could be discriminated.This nanopore device could simultaneously measure multiple nanoclusters in a mixture qualitatively and quantitatively.Furthermore,molecular dynamics(MD)simulations provided microscopic understandings of the nanocluster translocation dynamics and yielded 2D dwell time–current blockage spectra in close agreement with experiments.The nanopore platform provides a novel powerful tool for nanocluster characterization.

The efficacy and safety of vedolizumab in the treatment of 81 cases of active ulcerative colitis:a single center retrospective study

Objective To evaluate the efficacy and safety of vedolizumab(VDZ)in the treatment of active ulcerative colitis(UC).Methods From November 1,2020 to October 30,2022,at the Department of Gastroenterology,the Sixth Affiliated Hospital of Sun Yat-sen University,81 UC patients who received VDZ treatment and completed a 14-week follow-up were retrospectively selected.The clinical data of patients,including age,disease duration,disease activity of UC were collected.

Establishment and characterization of high-throughput culture platform for kidney organoids

Objective To establish and identify a highthroughput culture platform for induced pluripotent stem cells to differentiated kidney organoids.Methods Human urine-derived induced pluripotent stemcells were selected and plated at a suitable cell density.

Epicardial adipose tissue： the accomplice implicated in the genesis and maintenance of atrial fibrillation

Objective The purpose of this review was to delineate our current knowledge of the close relationship between the abundance of epicardial adipose tissue （EAT） and the risk of all major cardiovascular disease, especially atrial fibrillation （AF）. Data sources The data analyzed in this review were mainly from articles reported in PubMed published from 1972 to 2014. Study selection Original articles and critical reviews relevant to EAT and AF were selected. Results EAT, a particular form of metabolically active visceral fat deposited around the heart, is being regarded as an important independent predictor of cardio-metabolic diseases. EAT is composed of smaller adipocytes than other visceral fat depots and functioned like brown adipose tissue （BAT） to protect adjacent tissues. Improving the understanding of EAT in AF genesis and maintenance may contribute to prevent AF and reduce the complications associated with AF. Conclusion The findings suggest that EAT associates with AF severity and the recurrence of AF after catheter ablation even after adjustment forAF risk factors, but the precise mechanisms are not fully elucidated.

Rice Chitin Receptor OsCEBiP Is Not a Transmembrane Protein but Targets the Plasma Membrane via a GPI Anchor

Dear Editor,Plants are sessile organisms that are constantly challenged by microbial pathogens from the habitat. The key for plants to survive is to perceive pathogens in a timely and precise manner and subsequently launch multiple immune responses to counteract pathogenic infection. Pattern recognition receptors （PRRs）,

Nat Genet:科学家发现相同肿瘤中存在不同遗传复杂性的癌细胞

近日，一项刊登于国际杂志Namre Genetics上的研究报告中。来自美国洛杉矶希达-西奈（Cedars-Sinai）医学院的研究人员通过研究在食管肿瘤的组织样本中鉴别出了2000多个基因突变，从而详细阐述了癌症的复杂性。本文研究表明。单一肿瘤的不同区域或许具有多种遗传模式。