Analysis on Quality Control of Argyreia acuta Lour

[Objectives]To establish a method for caffeic acid content determination and thin-layer chromatography identification of the Zhuang medicine Argyreia acuta Lour.[Methods]Silica gel GF254(thin-layer plate),toluene-formic acid-ethyl acetate(V∶V∶V=5∶1.2∶3)(developing agent)and 365 nm ultraviolet light were used for thin-layer chromatography identification.Under the chromatographic conditions of column of Thermo SCIENTIFIC Hypersil C18(5μm,4.60 mm×250 mm),mobile phase of methanol-0.1%phosphoric acid(23∶77),detection wavelength of 320 nm,column temperature of 30℃,sample size of 10μL and flow rate of 1.0 mL/min,the content of caffeic acid in A.acuta Lour was determined.[Results]Caffeic acid can be detected in thin layer chromatography,with strong specificity and clear spots.When the sample size of caffeic acid is within the range of 0.0151-0.4530μg(R^(2)=0.9999),the content of caffeic acid showed a relatively good linear relationship with the peak area.The average recovery rate of A.acuta Lour was 99.94%(RSD=2.68%),97.56%(RSD=1.57%)and 99.79%(RSD=2.05%),respectively.[Conclusions]This method can effectively identify A.acuta Lour,and can accurately determine the content of caffeic acid in A.acuta Lour.It has characteristics of high accuracy,high precision,good color rendering stability,good reproducibility and fast analysis speed.

Causes of severe neonatal hyperbilirubinemia: a multicenter study of three regions in China

Background Available evidence suggests that our country bear great burden of severe hyperbilirubinemia.However,the causes have not been explored recently in different regions of China to guide necessary clinical and public health interventions.Methods This was a prospective,observational study conducted from March 1,2018,to February 28,2019.Four hospitals in three regions of China participated in the survey.Data from infants with a gestational age≥35 weeks,birth weight>2000 g,and total serum bilirubin(TSB)level≥17 mg/dL(342μmol/L)were prospectively collected.Results A total of 783 cases were reported.Causes were identified in 259 cases.The major causes were ABO incompatibility(n=101),glucose-6-phosphate dehydrogenase deficiency(n=76),and intracranial hemorrhage(n=70).All infants with glucose-6-phosphate dehydrogenase deficiency were from the central south region.Those from the central south region had much higher peak total bilirubin levels[mean,404μmol/L;standard deviation(SD),75μmol/L]than those from the other regions(mean,373μmol/L;SD,35μmol/L)(P<0.001).Conclusions ABO incompatibility was the leading cause in the east and northwest regions,but cases in the central south region were mainly caused by both ABO incompatibility and glucose-6-phosphate dehydrogenase deficiency,and infants in this region had a much higher peak total bilirubin level.Intracranial hemorrhage may be another common cause.More thorough assessments and rigorous bilirubin follow-up strategies are needed in the central south region.