Objective:To observe the effects of immunosuppressants triptolide(TL)and cyclosporineA(CSA)on HLA antigens expression induced by interferon-γ（INF-γ）in vitro.Method:By using an indirect immunofluorescent method and...Objective:To observe the effects of immunosuppressants triptolide(TL)and cyclosporineA(CSA)on HLA antigens expression induced by interferon-γ（INF-γ）in vitro.Method:By using an indirect immunofluorescent method and analysing with ACAS-570,the abnormal HLA antigen expression of cultured corneal epithelial cells was induced by INF-γ，After incubation with one of the immunosuppressants(CSA,TL) for72hrs.the amount of HLA-ABCand HLA-DRantigens was measured.Result:There was no significant difference(P>0.05)between the group with CAS and the positive control group without CAS.In contrast to CSA,TL dramatically inhibited INF-γinduced expression of HLA antigens of corneal epithelial cells(P<0.001),compared with the control group withoutTL.Conclusion:TL had direct inhibition on the expression of HLA-ABCand HLA-DRantige ns induced by INF-γin vitro,while CSAhad no obvious inhibition,Eye Science2000;16:34-37.展开更多
Objective:To observe the effects of immunosuppressants triptolide(TL) and cyclosporine A(CSA) on HLA antigens expression induced by interferon-γ(INF-γ)in vitro.Method:By using an indirect immunofluorescent method an...Objective:To observe the effects of immunosuppressants triptolide(TL) and cyclosporine A(CSA) on HLA antigens expression induced by interferon-γ(INF-γ)in vitro.Method:By using an indirect immunofluorescent method and analyzing with ACAS-570,the abnormal HLA antigen expression of cultured corneal epithelial cells was induced by INF-γ.After incubation with one of the immunosuppressants(CSA,TL) for 72 hrs,the amount of HLA-A BC and HLA-DR antigens was measured.Result:There was no significant difference(P>0.05) between the group with CSA and the positive control group without CSA.In contrast to CSA, TL dramatically inhibited IFN-γ induced expression of HLA antigens of corneal epithelial cells (P<0.001),compared with the control group without TL.Conclusion:TL had direct inhibition on the expression of HLA-ABC and HLA-DR antigens induced by INF-γ in vitro,while CSA had no obvious inhibition.Eye Science 2000;16:34-37.展开更多
文摘Objective:To observe the effects of immunosuppressants triptolide(TL)and cyclosporineA(CSA)on HLA antigens expression induced by interferon-γ（INF-γ）in vitro.Method:By using an indirect immunofluorescent method and analysing with ACAS-570,the abnormal HLA antigen expression of cultured corneal epithelial cells was induced by INF-γ，After incubation with one of the immunosuppressants(CSA,TL) for72hrs.the amount of HLA-ABCand HLA-DRantigens was measured.Result:There was no significant difference(P>0.05)between the group with CAS and the positive control group without CAS.In contrast to CSA,TL dramatically inhibited INF-γinduced expression of HLA antigens of corneal epithelial cells(P<0.001),compared with the control group withoutTL.Conclusion:TL had direct inhibition on the expression of HLA-ABCand HLA-DRantige ns induced by INF-γin vitro,while CSAhad no obvious inhibition,Eye Science2000;16:34-37.
文摘Objective:To observe the effects of immunosuppressants triptolide(TL) and cyclosporine A(CSA) on HLA antigens expression induced by interferon-γ(INF-γ)in vitro.Method:By using an indirect immunofluorescent method and analyzing with ACAS-570,the abnormal HLA antigen expression of cultured corneal epithelial cells was induced by INF-γ.After incubation with one of the immunosuppressants(CSA,TL) for 72 hrs,the amount of HLA-A BC and HLA-DR antigens was measured.Result:There was no significant difference(P>0.05) between the group with CSA and the positive control group without CSA.In contrast to CSA, TL dramatically inhibited IFN-γ induced expression of HLA antigens of corneal epithelial cells (P<0.001),compared with the control group without TL.Conclusion:TL had direct inhibition on the expression of HLA-ABC and HLA-DR antigens induced by INF-γ in vitro,while CSA had no obvious inhibition.Eye Science 2000;16:34-37.
