3D assessment of intervertebral disc degeneration in zebrafish identifies changes in bone density that prime disc disease

Back pain is a common condition with a high social impact and represents a global health burden.Intervertebral disc disease(IVDD)is one of the major causes of back pain;no therapeutics are currently available to reverse this disease.The impact of bone mineral density(BMD)on IVDD has been controversial,with some studies suggesting osteoporosis as causative for IVDD and others suggesting it as protective for IVDD.Functional studies to evaluate the influence of genetic components of BMD in IVDD could highlight opportunities for drug development and repurposing.By taking a holistic 3D approach,we established an aging zebrafish model for spontaneous IVDD.Increased BMD in aging,detected by automated computational analysis,is caused by bone deformities at the endplates.However,aged zebrafish spines showed changes in bone morphology,microstructure,mineral heterogeneity,and increased fragility that resembled osteoporosis.Elements of the discs recapitulated IVDD symptoms found in humans:the intervertebral ligament(equivalent to the annulus fibrosus)showed disorganized collagen fibers and herniation,while the disc center(nucleus pulposus equivalent)showed dehydration and cellular abnormalities.We manipulated BMD in young zebrafish by mutating sp7 and cathepsin K,leading to low and high BMD,respectively.Remarkably,we detected IVDD in both groups,demonstrating that low BMD does not protect against IVDD,and we found a strong correlation between high BMD and IVDD.Deep learning was applied to high-resolution synchrotron\iCJ image data to analyze osteocyte 3D lacunar distribution and morphology,revealing a role of sp7 in controlling the osteocyte lacunar 3D profile.Our findings suggest potential avenues through which bone quality can be targeted to identify beneficial therapeutics for IVDD.

A Model to Describe the Fracture of Porous Polygranular Graphite Subject to Neutron Damage and Radiolytic Oxidation

Two linked models have been developed to explore the relationship between the amount of porosity arising in service from both radiolytic oxidation and fast neutron damage that influences both the strength and the force-displacement(load-displacement)behaviour and crack propagation in pile grade A graphite used as a nuclear reactor moderator material.Firstly models of the microstructure of the porous graphite for both unirradiated and irradiated graphite are created.These form the input for the second stage,simulating fracture in lattice-type finite element models,which predicts force(load)-displacement and crack propagation paths.Microstructures comprising aligned filler particles,typical of needle coke,in a porous matrix have been explored.The purpose was to isolate the contributions of filler particles and porosity to fracture strength and crack paths and consider their implications for the overall failure of reactor core graphite.

Thermodynamic Formulation of Living Systems and Their Evolution II

In the previous paper [1], the application of the general thermodynamic theory was considered to biological systems. The nature of living matter has been presented from the mesoscopic to the macroscopic point of view, for different time scales (ontogenetic and phylogenetics). Herein, we continue with this application, and present three characteristics of life in the form of statements or postulates. The first characteristic describes the probability of survival against aging. In particular, the behaviour of life is shown as an independent mode of aging. The second characteristic refers to the adaptation of the species according to the environment. The relationship between the phenomenon of organic homeostasis and the origin of the clinical parameters that define health is highlighted. And finally, the third characteristic applies the principle of negentropy to describe evolution. A representative model is given as an example of each postulate.

A 3D In-vitro model of the human dentine interface shows long-range osteoinduction from the dentine surface

Emerging regenerative cell therapies for alveolar bone loss have begun to explore the use of cell laden hydrogels for minimally invasive surgery to treat small and spatially complex maxilla-oral defects.However,the oral cavity presents a unique and challenging environment for in vivo bone tissue engineering,exhibiting both hard and soft periodontal tissue as well as acting as key biocenosis for many distinct microbial communities that interact with both the external environment and internal body systems,which will impact on cell fate and subsequent treatment efficacy.Herein,we design and bioprint a facile 3D in vitro model of a human dentine interface to probe the effect of the dentine surface on human mesenchymal stem cells(hMSCs)encapsulated in a microporous hydrogel bioink.We demonstrate that the dentine substrate induces osteogenic differentiation of encapsulated hMSCs,and that both dentine andβ-tricalcium phosphate substrates stimulate extracellular matrix production and maturation at the gel-media interface,which is distal to the gel-substrate interface.Our findings demonstrate the potential for long-range effects on stem cells by mineralized surfaces during bone tissue engineering and provide a framework for the rapid development of 3D dentine-bone interface models.

REVIEW OF PARTICLE PHYSICS

The Review summarizes much of particle physics and cosmology. Using data from previous editions, plus 3,283 new measurements from 899 papers, we list, evaluate, and average measured properties of gauge bosons and the recently discovered Higgs boson, leptons, quarks, mesons, and baryons. We summarize searches for hypothetical particles such as heavy neutrinos, supersymmetric and technicolor particles, axions, dark photons, etc. All the particle properties and search limits are listed in Summary Tables. We also give numerous tables, figures, formulae, and reviews of topics such as Supersymmetry, Extra Dimensions, Particle Detectors, Probability, and Statistics. Among the 112 reviews are many that are new or heavily revised including those on： Dark Energy, Higgs Boson Physics, Electroweak Model, Neutrino Cross Section Measurements, Monte Carlo Neutrino Generators, Top Quark, Dark Matter, Dynamical Electroweak Symmetry Breaking, Accelerator Physics of Colliders, High-Energy Collider Parameters, Big Bang Nucleosynthesis, Astrophysical Constants and Cosmological Parameters. A booklet is available containing the Summary Tables and abbreviated versions of some of the other sections of this full Review. All tables, listings, and reviews （and errata） are also available on the Particle Data Group website： http ：//pdg. lbl. gov.

ELECTROWEAK MODEL AND CONSTRAINTS ON NEW PHYSICS

Revised November 2013 by J. Erler （U. Mexico） and A. Freit＆s （Pittsburgh U.）.10.1 Introduction 10.2 Renormalization and radiative corrections

ACCELERATOR PHYSICS OF COLLIDERS

Revised August 2013 by M.J. Syphers （MSU） and F. Zimmermann （CERN）.29.1. Luminosity This article provides background for the High-Energy Collider Parameter Tables that follow. The number of events, Nexp, is the product of the cross section of interest,

HIGHLIGHTS OF THE 2014 EDITION OF THE REVIEW OF PARTICLE PHYSICS

899 new papers with 3283 new measurements Over 330 papers from LHC experiments （ATLAS, CMS, and LHCb）.

STATUS OF HIGGS BOSON PHYSICS

Written November 2013 by M. Carena （Fermi National Accelerator Laboratory and the University of Chicago）, C. Grojean （ICREA at IFAE, Universitat Autbnoma de Barcelona）, M, Kado （Laboratoire de l＇Accelerateur Lineaire, LAL and CERN）, and V. Sharma （University of California San Diego）.

ONLINE PARTICLE PHYSICS INFORMATION

1. IntroductionThe collection of online information resources in particle physics and related areas presented in this chapter is of necessity incomplete. An expanded and regularly updated online version can be found at：

PARTICLE DETECTORS FOR NON-ACCELERATOR PHYSICS

Updated 2013 （see the various sections for authors）.34.1. Introduction Non-accelerator experiments have become increasingly important in particle physics. These include classical cosmic ray experiments, neutrino oscillation measurements, and searches for double-beta decay, dark matter candidates, and magnetic monopoles.

Bremsstrahlung emission from high power laser interactions with constrained targets for industrial radiography

Laser-solid interactions are highly suited as a potential source of high energy X-rays for nondestructive imaging.A bright,energetic X-ray pulse can be driven from a small source,making it ideal for high resolution X-ray radiography.By limiting the lateral dimensions of the target we are able to confine the region over which X-rays are produced,enabling imaging with enhanced resolution and contrast.Using constrained targets we demonstrate experimentally a(20±3)μm X-ray source,improving the image quality compared to unconstrained foil targets.Modelling demonstrates that a larger sheath field envelope around the perimeter of the constrained targets increases the proportion of electron current that recirculates through the target,driving a brighter source of X-rays.

BOTTOM MESONS(B=±1)B^+=u,B^0=d,B^0=db,B^-=b,similarly for B~*'s

Many measurements of B decays involve admixtures of B hadrons. Previously we arbitrarily included such admixtures in the B±section, but because of their importance we have created two new sections：

N BARYONS(S=0,I=1/2)p,N^+=uud;n,N^0=udd

p MASS （atomic mass units u）The mass is known much more precisely in u （atomic mass units） than in MeV. See the next data block.

BOTTOM,CHARMED MESONS(B=C=±1)B_c^+=c,B_c^-=cb,similarly for B_c~*'s

I（JP） = 0（0-）I, J, P need confirmation.Quantum numbers shown are quark-model predictions.

BOTTOM BARYONS(B=-1)∧_b^0=udb,≡_b^0=usb,≡_b^-=dsb,Ω_b^-=ssb

In the quark model, a ∧b0 is an isospin-0 udb state. The lowest ∧b0 ought to have JP = 1/2＋. None of I, J, or P have actually been measured.

INTRODUCTION

1. Overview The Review of Particle Physics and the abbreviated version, the Particle Physics Booklet, are reviews of the field of Particle Physics. This complete Review includes a compilation/evaluation of data on particle properties, called the ＂Particle Listings.＂ These Listings include 3,283 new measurements from 899 papers, in addition to the 32,153 measurements from 8,944 papers that first appeared in previous editions [1].

CHARMED BARYONS(C=+1)∧_c^+=udc,∑_c^(++)=uuc,∑_c^+=udc,∑_c^0=ddc,≡_c^+=usc,≡_c^0=dsc,Ω_c^0=ssc

CHARMED BARYONS Revised March 2012 by C.G. Wohl （LBNL）. There are 17 known charmed baryons, and four other candidates not well enough established to be promoted to the Summary Tables.＊ Fig. l（a） shows the mass spectrum,

PARTICLE DETECTORS AT ACCELERATORS

33.1. Introduction This review summarizes the detector technologies employed at accelerator particle physics experiments. Several of these detectors are also used in a non-accelerator context and examples of such applications will be provided. The detector techniques which are specific to non-accelerator particle physics experiments are the subject of Chap.

SU(3) ISOSCALAR FACTORS AND REPRESENTATION MATRICES

Written by R.L. Kelly （LBNL）. The most commonly used SU（3） isoscalar factors, corresponding to the singlet, octet, and deeuplet content of 8 8 and 10 8, are shown at the right. The notation uses particle names to identify the coefficients, so that the pattern of relative couplings may be seen at a glance. We illustrate the use of the coefficients below.