As a new diagnostic means, plasma-imaging system has been developed on the HL-2A tokamak, with a basic understanding of plasma discharge scenario of the entire torus, checking the plasma position and the clearance bet...As a new diagnostic means, plasma-imaging system has been developed on the HL-2A tokamak, with a basic understanding of plasma discharge scenario of the entire torus, checking the plasma position and the clearance between the plasma and the first wall during discharge. The plasma imaging system consists of (1) color video camera, (2) observation window and turn mirror, (3) viewing & collecting optics, (4) video cable, (5) Video capture card as well as PC. This paper mainly describes the experimental arrangement, plasma imaging system and detailed part in the system, along with the experimental results. Real-time monitoring of plasma discharge process, particularly distinguishing limitor and divertor configuration, the imaging system has become key diagnostic means and laid the foundation for further physical experiment on the HL-2A tokamak.展开更多
The effects of S86019, an active component from Puralia lobata, on the induction of cell differentiation and cell cycle traverse of HL-60 cells were described. It was shown that cell proliferation of HL-60 cells was i...The effects of S86019, an active component from Puralia lobata, on the induction of cell differentiation and cell cycle traverse of HL-60 cells were described. It was shown that cell proliferation of HL-60 cells was inhibited by S86019 in vitro. Under the action of S86019 the HL-60 cells were induced to differentiate into metamyelocytes, myelocytes and much matured cells with banded or segmented nucleus. Flow cytometry demonstrated that the cell population of HL-60 cells was blocked at G1 phase which resulted in the elevation of percentage of G1 cells and decrease of percentage of cells in S phase. Experimental results demonstrated that S86019 is an active inducer of cell differentiation in HL-60 cells.展开更多
1 Experiments HL-2A tokamak is characterized by a large closed divertor chamber. Before the experimental campaign 2005 the surface of the shielding plates for the MP1 and MP2 has been covered with 2-D carbon fibre co...1 Experiments HL-2A tokamak is characterized by a large closed divertor chamber. Before the experimental campaign 2005 the surface of the shielding plates for the MP1 and MP2 has been covered with 2-D carbon fibre composite （about 30% of the first wall）, which can protect the first wall, and effectively shield the splash of heavy metal impurity. The vacuum vessel can be baked up to 120 ℃for degassing and a glow discharge device is installed in the vessel for cleaning the inner surface. Hydrogen glow discharge cleaning （GDC） is applied to remove impurities from the wall, and helium glow discharge cleaning for removing residual hydrogen.展开更多
1 Introduction In the last two years＇ campaigns of the HL-2A experiments, single null （SN） divertor plasma was achieved. During the 2004 experiment, the supersonic molecular beam injection （SMB） and strong hydro...1 Introduction In the last two years＇ campaigns of the HL-2A experiments, single null （SN） divertor plasma was achieved. During the 2004 experiment, the supersonic molecular beam injection （SMB） and strong hydrogen gas puffing were used for gas fuelling, some unique phenomena have been observed. The plasma parameters achieved are： Ip=320 kA, ne=4.4× 10^19 m^ 3, By=2.2 T, plasma duration t=1580 ms.展开更多
During autologous bone marrow graft in treatment of malignant diseases, it is critical to purge malignant cells from the marrow. In the present study, the sensitivity to photodynamic inactivation of 3 leukemic cell li...During autologous bone marrow graft in treatment of malignant diseases, it is critical to purge malignant cells from the marrow. In the present study, the sensitivity to photodynamic inactivation of 3 leukemic cell lines was compared with their counterpart normal hematopoietic cells. After mouse leukemic L1210 cells were treated with a preparation of hematoporphyrin derivatives, YHpD, 10 μg/ml for 1 hr. and irradiated with blacklight (peak wavelength 395 nm, light intensity 0.6 mW/cm2) for 5 minutes, the survival rate of clonogenic cells decreased to <10%, while that of bone marrow granulocyte macrophage progenitor cells (CFU-GM) in DBA/2 mice remained at nearly normal level (>80%). Similar results were obtained when human leukemic HL-60 cells were compared with human CFU-GM and mouse leukemic L615 cells with CFU-GM in 615 strain mice. It is suggested that hematoporphyrin photoradiation may be useful for Iselectively killing leukemic cells in bone marrow.展开更多
The effects of inhibitors of TNFα converting enzyme (TACE) on TNFα secretion were studied to develop an approach to interfere inflammation processes. The HL-60 cell lines were stimulated in vitro with LPS intravenou...The effects of inhibitors of TNFα converting enzyme (TACE) on TNFα secretion were studied to develop an approach to interfere inflammation processes. The HL-60 cell lines were stimulated in vitro with LPS intravenously for different time to establish the cellular model of inflammation and simultaneously induce in vivo inflammation animal model by LPS The cytotoxic effects of soluble TNFa were checked using MTT colorimetric method to determine the rate of cell proliferation. The level of expression of TACE was detected by using RT-PCR, FCM and immuno-histochemical technique respectively. It was found Chinese medicine Reduqing (RDQ) could inhibit the transcription of TNFa mRNA induced by LPS stimulation (P<0. 01, compared with the control). The anti-oligodeoxyribonucleotide (anti-ODN) of TNFα mRNA could inhibit 78. 9 % of TNFα secretion. The mimic peptides of TACE substrates with hydroxamine group showed potency in vivo and in vitro a-gainst converting of pro-TNFα. It was concluded that all the three types of TACE inhibitors can regulate the expression of TACE at different levels and inhibit sTNFα secretion, indicating TACE is a novel target for inflammation therapy.展开更多
基金The project supported by the Tenth-Five-year Nuclear Energy Development of the Commission of Science Technology and Industry for National Defenseof the China National Nuclear Corporation
文摘As a new diagnostic means, plasma-imaging system has been developed on the HL-2A tokamak, with a basic understanding of plasma discharge scenario of the entire torus, checking the plasma position and the clearance between the plasma and the first wall during discharge. The plasma imaging system consists of (1) color video camera, (2) observation window and turn mirror, (3) viewing & collecting optics, (4) video cable, (5) Video capture card as well as PC. This paper mainly describes the experimental arrangement, plasma imaging system and detailed part in the system, along with the experimental results. Real-time monitoring of plasma discharge process, particularly distinguishing limitor and divertor configuration, the imaging system has become key diagnostic means and laid the foundation for further physical experiment on the HL-2A tokamak.
文摘The effects of S86019, an active component from Puralia lobata, on the induction of cell differentiation and cell cycle traverse of HL-60 cells were described. It was shown that cell proliferation of HL-60 cells was inhibited by S86019 in vitro. Under the action of S86019 the HL-60 cells were induced to differentiate into metamyelocytes, myelocytes and much matured cells with banded or segmented nucleus. Flow cytometry demonstrated that the cell population of HL-60 cells was blocked at G1 phase which resulted in the elevation of percentage of G1 cells and decrease of percentage of cells in S phase. Experimental results demonstrated that S86019 is an active inducer of cell differentiation in HL-60 cells.
文摘1 Experiments HL-2A tokamak is characterized by a large closed divertor chamber. Before the experimental campaign 2005 the surface of the shielding plates for the MP1 and MP2 has been covered with 2-D carbon fibre composite （about 30% of the first wall）, which can protect the first wall, and effectively shield the splash of heavy metal impurity. The vacuum vessel can be baked up to 120 ℃for degassing and a glow discharge device is installed in the vessel for cleaning the inner surface. Hydrogen glow discharge cleaning （GDC） is applied to remove impurities from the wall, and helium glow discharge cleaning for removing residual hydrogen.
文摘1 Introduction In the last two years＇ campaigns of the HL-2A experiments, single null （SN） divertor plasma was achieved. During the 2004 experiment, the supersonic molecular beam injection （SMB） and strong hydrogen gas puffing were used for gas fuelling, some unique phenomena have been observed. The plasma parameters achieved are： Ip=320 kA, ne=4.4× 10^19 m^ 3, By=2.2 T, plasma duration t=1580 ms.
文摘During autologous bone marrow graft in treatment of malignant diseases, it is critical to purge malignant cells from the marrow. In the present study, the sensitivity to photodynamic inactivation of 3 leukemic cell lines was compared with their counterpart normal hematopoietic cells. After mouse leukemic L1210 cells were treated with a preparation of hematoporphyrin derivatives, YHpD, 10 μg/ml for 1 hr. and irradiated with blacklight (peak wavelength 395 nm, light intensity 0.6 mW/cm2) for 5 minutes, the survival rate of clonogenic cells decreased to <10%, while that of bone marrow granulocyte macrophage progenitor cells (CFU-GM) in DBA/2 mice remained at nearly normal level (>80%). Similar results were obtained when human leukemic HL-60 cells were compared with human CFU-GM and mouse leukemic L615 cells with CFU-GM in 615 strain mice. It is suggested that hematoporphyrin photoradiation may be useful for Iselectively killing leukemic cells in bone marrow.
基金This project was supported by a grant from National Natural Sciences Foundation of China(No.30070722).
文摘The effects of inhibitors of TNFα converting enzyme (TACE) on TNFα secretion were studied to develop an approach to interfere inflammation processes. The HL-60 cell lines were stimulated in vitro with LPS intravenously for different time to establish the cellular model of inflammation and simultaneously induce in vivo inflammation animal model by LPS The cytotoxic effects of soluble TNFa were checked using MTT colorimetric method to determine the rate of cell proliferation. The level of expression of TACE was detected by using RT-PCR, FCM and immuno-histochemical technique respectively. It was found Chinese medicine Reduqing (RDQ) could inhibit the transcription of TNFa mRNA induced by LPS stimulation (P<0. 01, compared with the control). The anti-oligodeoxyribonucleotide (anti-ODN) of TNFα mRNA could inhibit 78. 9 % of TNFα secretion. The mimic peptides of TACE substrates with hydroxamine group showed potency in vivo and in vitro a-gainst converting of pro-TNFα. It was concluded that all the three types of TACE inhibitors can regulate the expression of TACE at different levels and inhibit sTNFα secretion, indicating TACE is a novel target for inflammation therapy.
