The polymerase chain reaction combined with direct sequencing was employed to deduce the variety of the grey-rumped pheasant populations in China.Totally 1079 basepair sequences of mitochondrial DNA (mtDNA) control-re...The polymerase chain reaction combined with direct sequencing was employed to deduce the variety of the grey-rumped pheasant populations in China.Totally 1079 basepair sequences of mitochondrial DNA (mtDNA) control-region gene were analyzed from 121 grey-rumped pheasants obtained from 13 Chinese localities, 76 variable sites resulted in 91 haplotypes.These populations possessed a high level of genetic diversity, indicating that during their evolution effective population size and polymorphism were not reduced by climatic fluctuations in the Pleistocene ice ages.Phylogenetic analysis indicates that all populations were clustered into two groups, western and eastern.The degree of population differentiation presumably results from a low gene flow (Nm=0.33, Fst=0.60, P<0.001) between the two groups, and the divergence occurred in the years (2.1×105 -2.2×105).The genetic structure of grey-rumped pheasants in China appears to be the result of the uplift of Qinghai-Tibet Plateau, the existence of Qinling Mountains and Liupan Mountains and cyclical climatic oscillations during the late Pleistocene.展开更多
基金Supported by Natural Science Foundation of Hainan Province(NO:311055)Doctoral Start-up Research Fund of Qiongzhou University(No:QYXB201009)+1 种基金Co-operation Fund between University and Locality of Sanya City(No:2010YD22)Fund for Zoology Major Course of Hainan Province
文摘The polymerase chain reaction combined with direct sequencing was employed to deduce the variety of the grey-rumped pheasant populations in China.Totally 1079 basepair sequences of mitochondrial DNA (mtDNA) control-region gene were analyzed from 121 grey-rumped pheasants obtained from 13 Chinese localities, 76 variable sites resulted in 91 haplotypes.These populations possessed a high level of genetic diversity, indicating that during their evolution effective population size and polymorphism were not reduced by climatic fluctuations in the Pleistocene ice ages.Phylogenetic analysis indicates that all populations were clustered into two groups, western and eastern.The degree of population differentiation presumably results from a low gene flow (Nm=0.33, Fst=0.60, P<0.001) between the two groups, and the divergence occurred in the years (2.1×105 -2.2×105).The genetic structure of grey-rumped pheasants in China appears to be the result of the uplift of Qinghai-Tibet Plateau, the existence of Qinling Mountains and Liupan Mountains and cyclical climatic oscillations during the late Pleistocene.
