Melon is an important horticultural crop with extensive diversity in many horticultural groups.To explore its genomic diversity,it is necessary to assemble more high-quality complete genomes from different melon acces...Melon is an important horticultural crop with extensive diversity in many horticultural groups.To explore its genomic diversity,it is necessary to assemble more high-quality complete genomes from different melon accessions.Meanwhile,a large number of QTLs have been mapped in several studies.Integration of the published QTLs onto a complete genome can provide more accurate information for candidate gene cloning.To address these problems,a telomere-to-telomere(T2T)genome of the elite melon landrace Kuizilikjiz(Cucumis melo L.var.inodorus)was de novo assembled and all the published QTLs were projected onto it in this study.The results showed that a high-quality Kuizilikjiz genome with the size of 379.2 Mb and N50 of 31.7 Mb was de novo assembled using the combination of short reads,PacBio high-fidelity long reads,Hi-C data,and a high-density genetic map.Each chromosome contained the centromere and telomeres at both ends.A large number of structural variations were observed between Kuizilikjiz and the other published genomes.A total of 1294 QTLs published in 67 studies were collected and projected onto the T2T genome.Several clustered,co-localized,and overlapped QTLs were determined.Furthermore,20 stable meta-QTLs were identified,which significantly reduced the mapping intervals of the initial QTLs and greatly facilitated identification of the candidate genes.Collectively,the T2T genome assembly together with the numerous projected QTLs will not only broaden the high-quality genome resources but also provide valuable and abundant QTL information for cloning the genes controlling important traits in melon.展开更多
调查瓜列当对新疆甜瓜的危害,探索瓜列当化学防控方法,为瓜列当防治措施制定及实施提供技术支撑。以新疆伽师县4个甜瓜主产乡镇为代表,调查瓜列当对甜瓜的危害程度及规律。结合新疆甜瓜栽培模式,进行2种除草剂各2种浓度对瓜列当的防治...调查瓜列当对新疆甜瓜的危害,探索瓜列当化学防控方法,为瓜列当防治措施制定及实施提供技术支撑。以新疆伽师县4个甜瓜主产乡镇为代表,调查瓜列当对甜瓜的危害程度及规律。结合新疆甜瓜栽培模式,进行2种除草剂各2种浓度对瓜列当的防治试验。调查的4个乡镇瓜列当都有不同程度的发生。甜瓜播种后50 d直到收获,都有瓜列当寄生危害。75%磺酰磺隆和甲基咪草烟都可用于对甜瓜列当的防治,磺酰磺隆效果优于甲基咪草烟。结合浇水冲施37.5 g a.i.·hm-275%磺酰磺隆,是最有效的新疆甜瓜瓜列当化学防治的施用方式和浓度。展开更多
以哈密瓜品种绿玫为试材,采用简约化栽培模式,设行距为225 cm,株距为50、60、70、80 cm 4个水平,比较分析不同栽培密度条件下哈密瓜坐果、产量及品质的差异。结果表明,在行距固定的条件下,适当加大株距,有利于提高哈密瓜产量和改善果实...以哈密瓜品种绿玫为试材,采用简约化栽培模式,设行距为225 cm,株距为50、60、70、80 cm 4个水平,比较分析不同栽培密度条件下哈密瓜坐果、产量及品质的差异。结果表明,在行距固定的条件下,适当加大株距,有利于提高哈密瓜产量和改善果实品质;在露地简约化栽培模式下,行距为225 cm时,绿玫适宜的栽培株距为60 cm。展开更多
基金This work was supported by the Key R&D Project of Hubei Province(2021BBA101)the Fundamental Research Funds for the Central Universities(2662020YLPY024)+2 种基金the Key R&D Project of Xinjiang Academy of Agricultural Sciences(xjkcpy-2022006)the Tianshan Innovation Team(2022D14015)the China Agriculture Research System(CARS-25).
文摘Melon is an important horticultural crop with extensive diversity in many horticultural groups.To explore its genomic diversity,it is necessary to assemble more high-quality complete genomes from different melon accessions.Meanwhile,a large number of QTLs have been mapped in several studies.Integration of the published QTLs onto a complete genome can provide more accurate information for candidate gene cloning.To address these problems,a telomere-to-telomere(T2T)genome of the elite melon landrace Kuizilikjiz(Cucumis melo L.var.inodorus)was de novo assembled and all the published QTLs were projected onto it in this study.The results showed that a high-quality Kuizilikjiz genome with the size of 379.2 Mb and N50 of 31.7 Mb was de novo assembled using the combination of short reads,PacBio high-fidelity long reads,Hi-C data,and a high-density genetic map.Each chromosome contained the centromere and telomeres at both ends.A large number of structural variations were observed between Kuizilikjiz and the other published genomes.A total of 1294 QTLs published in 67 studies were collected and projected onto the T2T genome.Several clustered,co-localized,and overlapped QTLs were determined.Furthermore,20 stable meta-QTLs were identified,which significantly reduced the mapping intervals of the initial QTLs and greatly facilitated identification of the candidate genes.Collectively,the T2T genome assembly together with the numerous projected QTLs will not only broaden the high-quality genome resources but also provide valuable and abundant QTL information for cloning the genes controlling important traits in melon.
文摘调查瓜列当对新疆甜瓜的危害,探索瓜列当化学防控方法,为瓜列当防治措施制定及实施提供技术支撑。以新疆伽师县4个甜瓜主产乡镇为代表,调查瓜列当对甜瓜的危害程度及规律。结合新疆甜瓜栽培模式,进行2种除草剂各2种浓度对瓜列当的防治试验。调查的4个乡镇瓜列当都有不同程度的发生。甜瓜播种后50 d直到收获,都有瓜列当寄生危害。75%磺酰磺隆和甲基咪草烟都可用于对甜瓜列当的防治,磺酰磺隆效果优于甲基咪草烟。结合浇水冲施37.5 g a.i.·hm-275%磺酰磺隆,是最有效的新疆甜瓜瓜列当化学防治的施用方式和浓度。
