Objective:To improve the knowledge on the metabolite profile of five red grapes from D?o region(Portugal),concerning to the phenolic characteristics(coloured and non-coloured phenolics) and organic acid composition.Me...Objective:To improve the knowledge on the metabolite profile of five red grapes from D?o region(Portugal),concerning to the phenolic characteristics(coloured and non-coloured phenolics) and organic acid composition.Methods:Five red grapes collected from D?o region were studied.The profiles of phenolic compounds and organic acids were estimated by high-performance liquid chromatography with diode-array detection and high-performance liquid chromatography with UV detector,respectively.Results:Totally 24 phenolic compounds were identified,and distributed by several classes:8 anthocyanins,1 hydroxybenzoic acid,4 hydroxycinnamic acids,1 stilbene,4flavan-3-ols,6 flavonols.Additionally,10 organic acids were detected in all samples.Total contents of each phenolic class and organic acids amounts varied significantly among the different grape cultivars investigated.The principal components analysis differentiates the Touriga Nacional from the other varieties due to their high contents in anthocyanins,non-coloured phenolics and organic acids.Touriga Nacional is an important red grape cultivar,highly esteemed in D?o region for its ability to produce high quality wines.Conclusions:The results suggest that the red grapes from D?o region present a good composition in bioactive compounds,being important for the production of wines with superior quality.展开更多
基金Supported by the European Union(FEDER funds through COMPETE)National Funds(FCT,Fundacao para a Cieencia e Tecnologia)through project Pest-C/EQB/LA0006/2013+1 种基金the European Union(FEDER funds)under the framework of QREN through Project NORTE-07-0124-FEDER-000069FCT the financial support for the Post-doc grant(SFRH/BPD/105263/2014)
文摘Objective:To improve the knowledge on the metabolite profile of five red grapes from D?o region(Portugal),concerning to the phenolic characteristics(coloured and non-coloured phenolics) and organic acid composition.Methods:Five red grapes collected from D?o region were studied.The profiles of phenolic compounds and organic acids were estimated by high-performance liquid chromatography with diode-array detection and high-performance liquid chromatography with UV detector,respectively.Results:Totally 24 phenolic compounds were identified,and distributed by several classes:8 anthocyanins,1 hydroxybenzoic acid,4 hydroxycinnamic acids,1 stilbene,4flavan-3-ols,6 flavonols.Additionally,10 organic acids were detected in all samples.Total contents of each phenolic class and organic acids amounts varied significantly among the different grape cultivars investigated.The principal components analysis differentiates the Touriga Nacional from the other varieties due to their high contents in anthocyanins,non-coloured phenolics and organic acids.Touriga Nacional is an important red grape cultivar,highly esteemed in D?o region for its ability to produce high quality wines.Conclusions:The results suggest that the red grapes from D?o region present a good composition in bioactive compounds,being important for the production of wines with superior quality.