Objective: To explore the mechanism of anti-rheumatic effect of the active fraction of Siegesbeckia pubescens (AFSP). Methods: Adjuvant arthritis (AA) model of rat was produced to observe the effect of AFSP on lymphoc...Objective: To explore the mechanism of anti-rheumatic effect of the active fraction of Siegesbeckia pubescens (AFSP). Methods: Adjuvant arthritis (AA) model of rat was produced to observe the effect of AFSP on lymphocyte proliferation, interleukin-1 and -2 (IL-1,-2) activity, pathologic section of ankle joint, and analgesic effect, in model rat. Results: AFSP could reduce the inflammatory pathologic response of ankle joint. It has good analgesic effect, the analgesic rate being 65%. AFSP could also strengthen T-lymphocyte proliferation, promote IL-2 activity and inhibit IL-1 activity. Compared with the control group, the difference was significant (P<0.01). Conclusion: By means of regulating the immune function of organism, AFSP could improve the local pathologic response to antagonize against rheumatism, therefore, it is an effective anti-rheumatism herbal medicine.展开更多
Background Nurr1 is a member of the nuclear receptor superfamily of transcription factors. The objective of the present study was to identify novel splicing variants of the gene in neuronal and non-neuronal tissues an...Background Nurr1 is a member of the nuclear receptor superfamily of transcription factors. The objective of the present study was to identify novel splicing variants of the gene in neuronal and non-neuronal tissues and determine their functions. Methods Reverse transcription-polymerase chain reaction (RT-PCR) analysis was used to screen for Nurr1 splice variants in the adult human central nervous system (CNS) and in other tissues such as lymphocytes,and liver,muscle,and kidney cells. Functional assays of the variants were performed by measuring Nurr1 response element (NuRE) transcriptional activity in vitro . Results In this study,the authors identified a novel splicing variant of Nurr1 within exon 5,found in multiple adult human tissues,including lymphocytes,and liver,muscle,and kidney cells,but not in the brain or spinal cord. Sequencing analysis showed the variant has a 75 bp deletion between nucleotides 1402 and 1476. A functional assay of the Nurr1-c splicing variant,performed by measuring NuRE transcriptional activity in vitro,detected a 39% lower level of luciferase (LUC) activity ( P <0.05).Conclusion A novel splicing variant of Nurr1 exists in human non-neuronal tissues and functional assays suggest that the variant may act as an alternate transcription regulator.展开更多
文摘Objective: To explore the mechanism of anti-rheumatic effect of the active fraction of Siegesbeckia pubescens (AFSP). Methods: Adjuvant arthritis (AA) model of rat was produced to observe the effect of AFSP on lymphocyte proliferation, interleukin-1 and -2 (IL-1,-2) activity, pathologic section of ankle joint, and analgesic effect, in model rat. Results: AFSP could reduce the inflammatory pathologic response of ankle joint. It has good analgesic effect, the analgesic rate being 65%. AFSP could also strengthen T-lymphocyte proliferation, promote IL-2 activity and inhibit IL-1 activity. Compared with the control group, the difference was significant (P<0.01). Conclusion: By means of regulating the immune function of organism, AFSP could improve the local pathologic response to antagonize against rheumatism, therefore, it is an effective anti-rheumatism herbal medicine.
文摘Background Nurr1 is a member of the nuclear receptor superfamily of transcription factors. The objective of the present study was to identify novel splicing variants of the gene in neuronal and non-neuronal tissues and determine their functions. Methods Reverse transcription-polymerase chain reaction (RT-PCR) analysis was used to screen for Nurr1 splice variants in the adult human central nervous system (CNS) and in other tissues such as lymphocytes,and liver,muscle,and kidney cells. Functional assays of the variants were performed by measuring Nurr1 response element (NuRE) transcriptional activity in vitro . Results In this study,the authors identified a novel splicing variant of Nurr1 within exon 5,found in multiple adult human tissues,including lymphocytes,and liver,muscle,and kidney cells,but not in the brain or spinal cord. Sequencing analysis showed the variant has a 75 bp deletion between nucleotides 1402 and 1476. A functional assay of the Nurr1-c splicing variant,performed by measuring NuRE transcriptional activity in vitro,detected a 39% lower level of luciferase (LUC) activity ( P <0.05).Conclusion A novel splicing variant of Nurr1 exists in human non-neuronal tissues and functional assays suggest that the variant may act as an alternate transcription regulator.
