The role of inositol 1,4,5-trisphosphate （IP3） in transducing heat-shock （HS） signals was examined in Arabidopsis. The whole-plant IP3 level increased within 1 min of HS at 37℃. After 3 min of HS, the IP3 level r...The role of inositol 1,4,5-trisphosphate （IP3） in transducing heat-shock （HS） signals was examined in Arabidopsis. The whole-plant IP3 level increased within 1 min of HS at 37℃. After 3 min of HS, the IP3 level reached a maximum 2.5 fold increase. Using the transgenic Arabidopsis plants that have AtHsp 18.2 promoter-β-glucuronidase （GUS） fusion gene, it was found that the level of GUS activity was up-regulated by the addition of caged IP3 at both non-HS and HS temperatures and was down-regulated by the phospholipase C （PLC） inhibitors {1-[6-（（ 1713-3-Methoxyestra-1,3,5（10）-trien- 7-yl）amino）hexyl]-2,5-pyrrolidinedione } （U-73122）. The intracellular-free calcium ion concentration （[Ca^2＋]i） increased during HS at 37℃ in suspension-cultured Arabidopsis cells expressing apoaequorin. Treatment with U-73122 prevented the increase of [Ca^2＋]i to some extent. Above results provided primary evidence for the possible involvement of IP3 in HS signal transduction in higher plants.展开更多
Embryogenic calli were induced from the mature seeds of the hexaploid semi-winter wheat. The embryogenic cell line was established, and then the friable calli suitable for suspension were induced by adjusting the cont...Embryogenic calli were induced from the mature seeds of the hexaploid semi-winter wheat. The embryogenic cell line was established, and then the friable calli suitable for suspension were induced by adjusting the content of reduced N and changing the 2,4DAA concentration. Protoplasts were isolated from the suspension cells and cultured(?)in KM8P and some other media. A great number of microcolonies were obtained. By altering media, the microcolonies were promoted to grow further and form compact or nodule-like calli. Intact plants were regenerated through organogenesis and embryogenesis on differentiation media.展开更多
基金This work was supported by the National Natural Science Foundation of China （No. 30270796） Natural Science Foundation of Hebei Province, China （No. C2005000171）.
文摘The role of inositol 1,4,5-trisphosphate （IP3） in transducing heat-shock （HS） signals was examined in Arabidopsis. The whole-plant IP3 level increased within 1 min of HS at 37℃. After 3 min of HS, the IP3 level reached a maximum 2.5 fold increase. Using the transgenic Arabidopsis plants that have AtHsp 18.2 promoter-β-glucuronidase （GUS） fusion gene, it was found that the level of GUS activity was up-regulated by the addition of caged IP3 at both non-HS and HS temperatures and was down-regulated by the phospholipase C （PLC） inhibitors {1-[6-（（ 1713-3-Methoxyestra-1,3,5（10）-trien- 7-yl）amino）hexyl]-2,5-pyrrolidinedione } （U-73122）. The intracellular-free calcium ion concentration （[Ca^2＋]i） increased during HS at 37℃ in suspension-cultured Arabidopsis cells expressing apoaequorin. Treatment with U-73122 prevented the increase of [Ca^2＋]i to some extent. Above results provided primary evidence for the possible involvement of IP3 in HS signal transduction in higher plants.
基金This work was supported in part by the Monsando Inc., USA.
文摘Embryogenic calli were induced from the mature seeds of the hexaploid semi-winter wheat. The embryogenic cell line was established, and then the friable calli suitable for suspension were induced by adjusting the content of reduced N and changing the 2,4DAA concentration. Protoplasts were isolated from the suspension cells and cultured(?)in KM8P and some other media. A great number of microcolonies were obtained. By altering media, the microcolonies were promoted to grow further and form compact or nodule-like calli. Intact plants were regenerated through organogenesis and embryogenesis on differentiation media.
