Influence of vegetation restoration on matrix structure and erosion resistance of iron tailings sites in eastern Hebei, China

The effects of vegetation restoration on matrix structure and erosion resistance of iron tailings were studied at dump sites in Malanzhuang, Qian'an, Hebei province, China. The restoration process involved soil spray sowing restoration model with Rhus typhina, soil and iron tailings admixture spray sowing restoration model with Amorpha fruticosa Linn. and six-hole brick restoration model with Pinus tabulaeformis Carriere.–Amorpha fruticosa Linn. mixed-forest, and direct restoration model with Hippophae rhamnoides and Sabina vulgaris. Results show that the composition and distribution of particles and aggregates were closely related to erosion resistance(P < 0.05), indicating that matrix structure of iron tailings play an important role in erosion resistance. The improvement in matrix structure of iron tailings by the different restoration models was in the order of R. typhina soil spray sowing > A. fruticosa soil and iron tailings admixture spray sowing > mixed-forest six-hole brick >H. rhamnoides direct restoration > S. vulgaris direct restoration. The R. typhina soil spray sowing restorationmodel resulted in the greatest improvement in matrix structure of iron tailings, increasing the clay(10.6%) and large particle aggregates(18.7%) contents significantly(P < 0.01). Simultaneously, particle population, grading conditions(Cu= 28.86, Cs= 1.65), and aggregate stability(6.02) were significantly improved. The A. fruticosa soil and iron tailings admixture spray sowing restoration model,which effectively improved particle distribution(Cu-= 8.51, Cs= 1.07), increased the number of large aggregates considerably(9.6%), thereby increasing aggregate stability(6.2). The six-hole brick model significantly increased the number of large aggregates(4.0%) and improved the stability of aggregates(6.2). H. rhamnoides direct restoration improved the stability of aggregates(5.1)but showed no other significant improvements. The effect of S. vulgaris direct restoration on matrix structure of iron tailings was not significant. Due to its dependence on matrix structure of iron tailings, the erosion resistance of R.typhina soil spray sowing restoration model was the greatest, while that of S. vulgaris direct restoration was the weakest. There was no significant difference in the erosion resistance of the other models. Overall, vegetation restoration supplemented by soil spray sowing restoration and engineering measures is superior to in situ direct vegetation restoration in the short-term. In-situ direct restoration has long-term ecological significance because of its advanced concept of near-natural restoration and the advantages of low cost, easy operation, and low secondary damage.

Demonstration and Radiation Extension mode of Agricultural Technology——A Case of the Science and Technology Project of High Yield in Hebei Province

Firstly,relying on the science and technology project of high yield in Hebei Province,connotation of agricultural technology demonstration and extension mode and the high yield grain project in Hebei Province is introduced.Extension mode of agricultural technology demonstration and radiation is constructed.Agricultural technology demonstration and radiation mode includes the radiation center,primary irradiation and secondary irradiation.Secondly,management system and operational mechanism of agricultural technology demonstration and radiation mode are discussed,mainly expressed in establishing leading group in each demonstration county(city),carrying out leader contract responsibility system,establishing expert advisor steering group and setting up core experts group for subject,establishing technical experts group for subject,setting up leading group in the radiation area,carrying out chief expert responsibility system and technician matrix responsibility system,establishing the operating mechanism of "open,flow,competition and collaboration" with "test area-expert two-way selection" as the core content.Finally,countermeasures to improve the agricultural technology demonstration and radiation mode is put forward,such as establishing the rural technology demonstration base,strengthening cooperation with enterprises,and adopting flexible technical training,so as to promote the spread of agricultural high-tech,to increase the contribution rate of agricultural technology,and to offer ideas for agricultural technology extension model at the new era.

Empirical research on impacted factor of farmer＇s willingness for purchasing information Based on the survey data of Hebei province

Farmer＇s willingness for purchasing information, an important factor of farmer＇s information demand, was analyzed by logit model, according to the survey data of 535 farmers from eleven cities in Hebei province. The result showed that 6 factors had a significant influence on it, that is, education of farmers, per capita income of farmers, the affordability of information risk, the proportion of agricultural labor households, the type of agricultural production and the rural information service stations. On the basis of these, the related policy recommendations were proposed.

Cloning and sequence analysis of E2 gene of bovine viral diarrhea virus HB-DCZ strain in Hebei province of China

The objective of this paper was to analyze the E2 genetic characterization of HB-DCZ strain of Bovine viral diaxrhca Virus （BVDV） which wcrc amplified by RT-PCR and isolated from China. The product of PCK was cloned into pMD18-T vector, and then transfected Escherichia Coli JMI00. The recombinant plasmids were amplified by PCR and were sequenced. From the nucleotide sequence of the amplified products, phylogenetie analyses were performed and genotypes or subgenotypes were identified. The results indicated that the E2 gene fragment of HB-DCZ strain contained 1277bp nucleotides, and had 89.4%, 70.7%, 97.6%, 68.9%, 67.2% sequence similarity with Osloss, OregonC24V, Changchun184, ZM195, NADL, respectively. In conclusion, HB-DCZ strain is closely related to BVDV Osloss, Changchun184, and belongs to subgenotype lb.

Empirical Analysis of the Vegetable Industry in Hebei Province

We first introduce the status quo of the development of vegetable industry in Hebei Province,and then conduct empirical analysis of the development of vegetable industry in Hebei Province.Further,we analyze the development advantage of the vegetable industry in Hebei Province using SAI(Scale Advantage Indices) and SCA(Symmetric Comparative Advantage),drawing the conclusion that the vegetable industry in Hebei Province has much room for development;at the same time,we analyze the factors influencing vegetable consumption of residents in Hebei Province through the regression model,drawing the conclusion that the vegetable consumer price index is the main factor affecting the consumption.Finally we make recommendations for the development of vegetable industry in Hebei Province as follows:increasing financial input,promoting policy guarantee capacity;implementing brand strategy,promoting the competitiveness of products;improving the ecological environment,promoting industrialization of pollution-free vegetables.

Empirical study on evaluation of regional agri-food logistics capability in e-commerce environment based on factor analysis A case study of Hebei province

With SPSS16.0 software, this paper chooses the statistical data of 11 cities in Hebei province to empirically study the agri-food logistics capability based on the method of factor analysis, and finally obtains the ranking of the 11 cities. It shows that, factor-cluster analysis is an effective method to analyze the logistics capability of agri-food. It can simplify the original complicated problem and lead to an objective, reliable and convincing conclusion.

Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line‘AT7–4'

Flowering time is an important agronomic trait of Chinese cabbage with late flowering being a primary breeding objective.In our previous work,we obtained Chinese cabbage-cabbage translocation lines that contained several beneficial cabbage genes.Cabbage-specific molecular markers show that these genes were coming from chromosome C01 of cabbage.In this study,we investigated the inheritance of flowering time in a couple of translocation lines and analyzed the transmission rate of molecular markers in the offspring.Consequently,we obtained the late flowering Chinese cabbage-cabbage translocation line‘AT7–4’in which the flowering time was later than that of‘85–1’by about 7 days under 4-week vernalization.Based on previous studies of the genomes of Chinese cabbage and cabbage,we located the cabbage-specific molecular markers that were closely linked at the top of the chromosome A01 in the F2mapping population generated by self-crossing F1s derived from a cross between the translocation line‘AT7–4’and Chinese cabbage‘14–36’.Five flowering-related genes in the alien fragment were found by functional annotation and their molecular markers were developed.This study lays the foundation for the future improvement of Chinese cabbage varieties using A-C translocation lines.

小麦遗传转化研究进展(英文)

Gradual progress made in genetic transformation of wheat is presented in this paper. Information on promoters, antibiotic, herbicide and auxotrophic markers, and various traits of wheat modified through genetic transformation, is provided. In addition the methods used for wheat transformation are discussed. Though significant efforts have been made for genetic transformation of wheat mainly through particle bombardment method but transformation efficiency is still low for mass production of fertile transgenic plants. Studies on the inheritance of transgenes and its incorporation into commercial elite cultivars are not significant. Agrobacterium mediated transformation seems to have better prospects for wheat transformation in future due to its advantages over particle bombardment. In planta transformation of wheat tissues seems possible only with Agrobacterium.

Study on Supercooling Point and Freezing Point in Floral Organs of Apricot

Under the environment of an artificial climate chamber, supercooling point （SCP） and freezing point （FP） in flower and young fruit at different development stages and freezing injuries of floral organs were studied. The apricot cultivars tested were Kety, Golden Sun and Honghebao. With the development of flower buds, SCP and FP increased, which indicated that their cold resistance decreased. SCP and FP varied with different floral organs. For different apricot cultivars, it was found that, the lower SCP or FP in floral organs was, the more resistant capacity the cultivar had, and the larger the temperature interval from SCP to FP was. SCP was not a constant value, but a range. Frequency distribution of SCP in petals was more dispersing than that in stamens and pistils. Floral organs could maintain a supercooling state to avoid ice formation, but they were sensitive to freezing. Once floral organs froze, thev turned brown after thawing.

Effects of cadium, zinc and lead on soil enzyme activities

Heavy metal （HM） is a major hazard to the soil-plant system. This study investigated the combined effects of cadium （Cd）, zinc （Zn） and lead （Pb） on activities of four enzymes in soil, including calatase, urease, invertase and alkalin phosphatase. HM content in tops of canola and four enzymes activities in soil were analyzed at two months after the metal additions to the soil. Pb was not significantly inhibitory than the other heavy metals for the four enzyme activities and was shown to have a protective role on calatase activity in the combined presence of Cd, Zn and Pb; whereas Cd significantly inhibited the four enzyme activities, and Zn only inhibited urease and calatase activities. The inhibiting effect of Cd and Zn on urease and calatase activities can be intensified significantly by the additions of Zn and Cd. There was a negative synergistic inhibitory effect of Cd and Zn on the two enzymes in the presence of Cd, Zn and Pb. The urease activity was inhibited more by the HM combinations than by the metals alone and reduced approximately 20%--40% of urease activity. The intertase and alkaline phosphatase activities significantly decreased only with the increase of Cd concentration in the soil. It was shown that urease was much more sensitive to HM than the other enzymes. There was a obvious negative correlation between the ionic impulsion of HM in soil, the ionic impulsion of HM in canola plants tops and urease activity. It is concluded that the soil urease activity may be a sensitive tool for assessing additive toxic combination effect on soil biochemical parameters.

Effect of Phopshate Fertilizer and Manure on Crop Yield, Soil P Accumulation, and the Environmental Risk Assessment

Phosphorus （P） applied from fertilizer and manure is important in increasing crop yield and soil fertility; however, excessive uses of phosphate fertilizer and manure may also increase P loss from agricultural soils, posing environmental impact. A long term experiment was conducted on a calcareous soil （meadow cinnamon） in Hebei Province, China, from 2003 to 2006 to investigate the effects of phosphate fertilizer and manure on the yield of Chinese cabbage, soil P accumulation, P sorption saturation, soluble P in runoff water, and P leaching. P fertilizer （P2O5） application at a rate of 360 kg ha^-1 or manure of 150 t ha^-1 significantly increased Chinese cabbage yield as compared to the unfertilized control. However, no significant yield response was found with excessive phosphate or manure application. Soil Olsen-P, soluble P, bioavailable P, the degree of phosphorus sorption saturation in top soil layer （0-20 cm）, and soluble P in runoff water increased significantly with the increase of phosphate fertilizer and manure application rates, whereas the maximum phosphorus sorption capacity （Qm） decreased with the phosphate fertilizer and manure application rates. Soil Olsen-P and soluble P also increased significantly in the sub soil layer （20-40 cm） with the high P fertilizer and manure rates. It indicates that excessive P application over crop demand can lead to a high environmental risk owing to the enrichment of soil Olsen-P, soluble P, bioavailable P, and the degree of phosphorus sorption saturation in agricultural soils.

Functional Analysis of MYB73 of Arabidopsis thaliana Against Bipolaris oryzae

Bipolaris oryzae is a severe rice disease,resulting in significant economic losses.MYB73 gene of Arabidopsis was isolated based on T-DNA insertion mutants whose lose-of-function mutant increased susceptibility to B.oryzae.Results of staining for H2O2 revealed that infection of incompatible B.oryzae caused much more strong brown patches on the leaves of myb73 mutant than those on the leaves of wild type.Expression of MYB73 gene was induced by B.oryzae.Its increased expression was severely impaired in the myb73 mutant.Expression of MYB73 was severely decreased in the npr1,jar1,eds5,and sid2 mutants,suggesting MYB73 gene participates in the jasmonate （JA） and salicylic acid （SA） signaling pathways.Expression of MYB73 who encoded an R2R3 MYB transcription factor was increased by SA,JA,and ethylene （ET） treatments.The cDNA full-length sequence of MYB73 was 960 bp and a protein with 320 amino acids was encoded.The predicted molecular weight of MYB73 was 34.85 kDa.The expression of MYB73 gene was induced within 24 h of the inoculation,however,the expression of PR1,PDF1.2 and NPR1 weren＇t changed.When B.oryzae successfully infected myb73 mutant plants,the expression of PR1,PDF1.2 and NPR1 were increased.Collectively,our results suggested that MYB73 is involved in NPR1-mediated SA and JA signaling pathways.

Novel method for the determination of five carbamate pesticides in water samples by dispersive liquid-liquid microextraction combined with high performance liquid chromatography

A novel method for the determination of five carbamate pesticides （metolcarb, carbofuran, carbaryl, isoprocard and diethofencard） in water samples was developed by dispersive liquid-liquid microextraction （DLLME） coupled with high performance liquid chromatography-diode array detector （HPLC-DAD）. Some experimental parameters that influence the extraction efficiency were studied and optimized to obtain the best extraction results. Under the optimum conditions for the method, the calibration curve was linear in the concentration range from 5 to 1000 ng mL^-1 for all the five carbamate pesticides, with the correlation coefficients （r^2） varying from 0.9984 to 0.9994. Good enrichment factors were achieved ranging from 80 to 177- fold, depending on the compound. The limits of detection （LODs） （S/N = 3） were ranged from 0.1 to 0.5 ng mL^-1. The method has been successfully applied to the analysis of the pesticide residues in environmental water samples.

Molecular Characterization and Expression Analysis of TaZFP15, a C_2H_2-Type Zinc Finger Transcription Factor Gene in Wheat (Triticum aestivum L.)

Based on sequencing of part clones in a root subtractive cDNA library, an expressed sequence tag （EST） sharing high similarity to a rice C2H2 zinc finger transcription factor （ZFP15） was obtained in wheat. Through bioinformatics approach, the wheat C2H2-type ZFP gene referred to TaZFP15 has been identified and characterized. As a full-length cDNA of 670 bp, TaZFP15 has an open reading frame of 408 bp and encodes a 135-aa polypeptide. TaZFP15 contains two C2H2 zinc finger domains and each one has a conserved motif QALGGH. The typical L-box, generally identified in the C2H2 type transcription factors, has also been found in TaZFP15. Phylogenetic analysis suggested that TaZFP15 shares high similarities with rice ZFP15 （GenBank accession no. AY286473）, maize ZFP （GenBank accession no. NM_001159094） and a subset of other zinc-finger transcription factor genes in plant species. The expression of TaZFP15 was up-regulated by starved-Pi stress, showing a pattern to be gradually elevated along with the progression of the Pi-stress in a 23-h treatment regime. Similarly, the transcripts of TaZFP15 in roots were also induced by nitrogen deficiency, and abiotic stresses of drought and salinity. No responses of TaZFP15 were detected in roots to nutrition deficiencies of P, Zn, and Ca, and the external treatment of abscisic acid （ABA）. TaZFP15 could be specifically amplified in genome A, B, and D, and without variability in the sequences, suggesting that TaZFP15 has multi-copies in the homologous hexaploid species. Transgenic analysis in tobacco revealed that up-regulation of TaZFP15 could significantly improve plant dry mass accumulation via increasing the plant phosphorus acquisition capacity under Pi-deficiency condition. The results suggested that TaZFP15 is involved in mediation of signal transductions of diverse external stresses.

Effects of Dietary Energy Level on the Expression of the HSL Gene in DifferentTissues of Sheep

A total of 36 four-mon-old hybrid lambs （Dorset×Thin-tailed Han sheep） with similar body weight （BW） were randomly allocated to three dietary treatments with different energy （7.21, 10.33 and 13.49 MJ d-1 ME） but similar protein levels. The animals were slaughtered and subcutaneous fat, longissimus dorsi muscle, femoral biceps muscle and cardiac muscle tissue samples were taken after being treated for 40 d. The samples were then subjected to quantitative PCR to determine mRNA expression of hormone-sensitive lipase （HSL） in different tissues in the laboratory. The findings showed that the abundance of HSL mRNA decreased with the elevation of dietary energy. In the subcutaneous fatty tissue, the HSL mRNA levels showed significant differences among the three groups （P〈0.01）; in the longissimus dorsi and femoral biceps muscles, the HSL mRNA level in the low energy group was significantly higher than that in the moderate and high energy groups （P〈0.01）. In the cardiac muscle, the HSL mRNA level in the moderate energy group was significantly different from the low and high energy groups （P〈0.05）. The number of HSL copies （Qty） in different tissues of sheep was different, it was greater in the subcutaneous fat than in longissimus dorsi muscle, femoral biceps muscle and heart.

Isolation and Structural Indentification of Herbicidal Toxin Fractions Produced by Pythium aphanidermatum

In order to understand the compsition and structure of herbicidal component of Pythium aphanidermatum,the isolation and structural indentification were researched.The culture filtrate was extracted by ethyl acetate,petroleum,and chloroform with the same volume respectively and the activity of the crude toxin was bioassayed.The toxin was separated by using the method of thin layer chromatography（TLC）,then the main fraction was separated by HPLC,and the structure was analyzed by the sepctrum of IR,13C-NMR and 1HNMR.The results showed that the ethyl acetate extracts had the strongest herbicidal activity.Using the method of TLC,the bioassay results showed that the extracts with Rf 0.19 had the strongest effect on weeds and the inhibition to Digitaria sanguinalis and Amaranthus retroflexus reached five levels,and the component was proved to be dimethyl o-phthalate from the spectrum of IR,13C-NMR and 1HNMR,which was one of the components from the toxin,and it had herbicidal activity.

Cloning and Expression of One Fibrinolytic Enzyme from Bacillus sp. zlw-2

The gene encoding fibrinolytic enzyme from Bacillus sp. zlw-2 was cloned and sequenced （accession no. EU734749）, which was 1146 bp, encoded 381 amino acids and had 99% homology with Nattokinase YF308 and NAT. The genes encoding pre-pro-fibrinolytic enzyme （including signal peptide, propeptide, and mature peptide） and fibrinolytic enzyme （including mature peptide） were cloned into pET28a vector respectively and then transformed into Escherichia coli BL21 （DE3）. The recombinant ofpre-pro-fibrinolytic enzyme showed enzyme activity of 183 U mL^-1, while no detectable enzyme activity could be found from the recombinant of the mature peptide.

Optimization and Characterization of Nicosulfuron-Degrading Enzyme from Bacillus subtilis Strain YB1

A strain of Bacillus subtilis strain YB 1, isolated and preserved in our lab., showed a high nicosulfuron-degrading activity. Optimization of culture conditions on production of nicosulfuron-degrading enzyme from Bacillus subtilis strain YB 1 was carried out through mono-factor experiments. The characterization of degrading enzyme（s） was studied in this paper. The results showed that B. subtilis YB1 can use nicosulfuron as sole carbon source under aerobic condition. The key enzyme（s） involved in the initial biodegradation of nicosulfuron was localized to extracellular proteins and showed to be induced expressed. Enzyme-specific activity was up to 89.34 U mg-1 at pH 8.0 and 30℃, incubation for 96 h, inoculum 4.5x108 CFU mL-1 in Luria-Bertani liquid medium with nicosulfuron of 40 mg L-1. The maximum degradation rate of extracellular crude enzymes on nicosulfuron was 66% at pH 9.0, 35℃ in the enzymatic reaction system with nicosulfuron of 5 mg L-1. This degrading enzyme（s） was sensitive to high temperature, but kept high activity under alkaline conditions.

Advances in Transgenic Techniques in Apple

Main progress of transgenic techniques in apple made in recent years was summarized, and related research achievements in various aspects like transformation method, regenerating ability of explants, strain type and infection conditions of Agrobacterium were reviewed. On this basis, the current bottlenecks in transgenic techniques in apple were put forward.

Monosomic Addition Lines of Flowering Chinese Cabbage(B.campestris L.ssp.chinensis var.parachinensis L.H.Bailey)-Chinese Kale(B.oleracea L.var.alboglabra L.H.Bailey)

Interspecific alien addition lines have played significant roles in gene mapping, intergenomic gene transfer and chromosomal homoeological identification between closely related species. Selection of alien addition lines was conducted by karyotype analysis and morphological observation with the reference of parents. Triploid interspecies hybrid （AAC, 2n = 3x = 29） was obtained from Brassica campestris ssp. chinensis var. parachinensis Qinglu 9601 （tetraploid, AAAA, 2n = 4x = 40） x B. oleracea var. alboglabra Baihua 9705 （diploid, CC, 2n = 2x = 18） by immature hybrid embryo culture in vitro. Five different alien monosomic addition lines （AA ＋ C2, AA ＋ C3, AA ＋ C4, AA ＋ C6, AA ＋ C7） were obtained from the backcross progenies of AAC x AA. Each alien monosomic addition line has some specific morphological characters. It is feasible to obtain alien addition lines from the progenies of AAC × AA by karyotype analysis and morphological observation based on the reference of parents