In Vitro Antibacterial Effect of Traditional Chinese Medicine on Main Pathogenic Bacteria of Recessive Mastitis in Dairy Cows

The inhibition zone diameter, minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of 20 kinds of traditional Chi- nese medicine on Staphylococcus aureus and Streptococcus agalactiae isolated from recessive mastitis were studied using plate test and tube test. The results showed that S. aureus and S. agalactiae were sensitive to all these 20 kinds of traditional Chinese medicine, and were more sensitive to seven kinds of traditional Chinese medicine, including Taraxtwum mongolicum, Coptis chinensis, Forsythia suspense, Andrographis paniculata, Carthamus tinctorius, Scutellaria baicalensis and Den- dranthema indicum.

Effects of Echinacea Polysaccharide on Expression of NF-κB Protein Secreted by LPS-injured IEC-6 Cells

[ Objective ] The paper was to explore effects of Echinacea polysaccharide （EPS） on expression of NF-KB protein secreted by LPS-injured IEC-6 cells, and to provide a theoretical basis for clinical application of Echinacea purpurea against bacterial diseases and enhancement of immunity. [ Method] Nucleoprotein extracted from IEC-6 cells in normal control group, LPS group, different concentrations of EPS （50, 100,200,500 μg/mL） ＋ LPS groups were detected by SDS- PAGE electrophoresis, and the content of NF-κB protein was analyzed using western blotting method. [ Result ] The content of NF-KB protein in normal control group was the lowest, while that in LPS group was the highest. The content of NF-κB protein in EPS group gradually decreased with the increasing concentration of EPS. [ Result] The expression of NF-κB protein increased when IEC-6 cells were stimulated by LPS, and EPS could effectively inhibit increased expression of NF- κB protein. With the increasing concentration of EPS, the inhibition effect against increased expression of NF-κB protein gradually strengthened.

Influences of Echinacea Polysaccharide on Secretion of IL-8mRNA by LPS-injured IEC-6 Cells

[ Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8mRNA by LPS-injured IEC-6 cells, in order to figure out the mechanism of EPS on injured cells. MethodI Total RNA was extracted with TRlzon reagent. IL-8 mRNA was amplified by RT-PCR and detected by agar- ose gel electrophoresis. Furthermore, electrophoresis and image was analyzed. [ Result] The 50 μg/mL EPS could partially inhibit IL-8 mRNA level produced by -stimulated IEC-6; with the increasing concentration of EPS （200 and 500 μg/mL） , the inhibitory effect against IL-8 mRNA gradually enhanced. IEC-6 was pretreated by 50, 100,200 and 500 μg/mL EPS for ：24 h, then stimulated by 10 μg/ml 1,PS for 1 and 4 h, respectively. RT-PCR analysis showed that the expres- sion of 1L-8 mRNA induced by LPS was effectively inhibited by EPS; the inhibitory effect of EPS on expression of IL-8mRNA after stimulated by LPS for 4 h was more intense and the expression concentration was lower; the inhibitory rate at 4 h was higher than that at I h. [ Conclusion] EPS protected intestinal mucosa by inhibiting secretion of IL-8 mRNA by LPS-stimulated cells, and the inhibition of EPS was dependent to concentration and time.