Research Progress in the Treatment of Poststroke Depression with Traditional Chinese Medicine

In recent years,the incidence rate of stroke has increased year by year.Post stroke depres‐sion is one of its main complications,which seriously affects the recovery of physiological functions and quality of life of stroke patients.Traditional Chinese medicine has signifi‐cant therapeutic effects in treating this disease.This article provides a classification and review of traditional Chinese medicine treatment methods for post-stroke depression,and looks forward to the current problems.

Study on medication rules of traditional Chinese medicine for Meniere's disease based on data analysis

Objective:Study on medication rules of traditional Chinese medicine in treating Meniere's disease based on data mining technology.Methods:Computer retrieval with since establishment of the“CNKI”,“WF”,“VIP”,there have been literatures on the treatment of Meniere syndrome with Traditional Chinses medicine.Memory preprocessing in accordance with inclusion criteria.Then,EXCLE 2010,SPSS Statistics(ver.25)and SPSS Modeler(ver.18.0)were adopted respectively for frequency analysis,cluster analysis and association rules analysis.Result:A total of 133 references were included in this study,146 prescriptions,192 kinds of drugs,and the total frequency of drugs was 1702.The high frequency Meniere syndrome types were spleen deficiency and phlegm dampness syndrome,wind phlegm syndrome,gallbladder depression and phlegm disturbance syndrome,hyperactivity of liver yang syndrome,phlegm and blood stasis syndrome.The efficacy of drug frequency≥25 were summarized as follows,antiasthmatic drugs for relieving phlegm and relieving cough,diuresis-removing dampness,Tonic drugs,etc.High frequency meridian of drugs are:lung,liver,spleen,etc.High frequency drug properties are:temperature,cold,flat etc.High frequency drug taste:bitter,sweet,pungent,etc.Core drugs:Pinellia,Atractylodes,Poria,Gastrodia,etc.The main prescriptions werealisma soup,Banxia Baizhu Tianma Decoction,Zhengan Xifeng Decoction and Xiaochaihu Decoction.Conclusion:In this study,data mining was used to sort out the treatment of Meniere's disease by traditional Chinese medicine.It was found that the treatment of Meniere's disease was mainly to calm the liver and strengthen the spleen,supplemented by regulating qi and activating blood,clearing heat and opening orifices,tonifying deficiency and tranquilizing mind.

Meta-analysis of randomized controlled trials of traditional Chinese medicine in the treatment of obstructive sleep apnea syndrome

Objective:Though Chinese medicine(CM)has showed its clinical efficacy for the treatment of obstructive sleep apnea(OSA)in China,no systematic reviews or meta-analyses provide evidences for its therapeutic effects on OSA and the long-term safety.The aim of the present study is to evaluate the effectiveness and safety of CM on OSA using meta-analysis.Methods:We used search items of"Chinese Medicine"AND"obstructive sleep apnea"to retrieve the randomized control trials(RCTs)of CM treatments for OSA in PubMed,Embase,Cochrane Library,China National Knowledge Infrastructure(CNKI),VIP database,and Wanfang database from their respective inception dates to December 2019.Only RCTs of CM therapy versus no treatment which could be quantitatively synthesized were included.Finally,20 studies representing 1,297 participants were included after extraction.Two investigators independently extracted and analyzed the data using RevMan5.3 software.Results:The treatment group using CM decoctions or CM granules presented significantly ameliorative effects on apnoea-hypopnoea index(AHI)compared with the control group(MD:-2.58,95%CI:-3.59 to-1.56,P<0.00001 and MD:-5.47,95%CI:-6.75 to-4.19,P<0.00001,respectively)in the sensitivity analysis.However,there were non significant differences in the duration of treatment between subgroups,indicating that the duration of treatment has no impacts on the therapeutic effects on AHI.CM granules also showed significantly ameliorative effects on the lowest nocturnal oxygen saturation(LSaO2)(MD:2.76,95%CI:1.85 to 3.68,P<0.00001).CM decoctions exhibited significantly improved Epworth Sleepiness Scale(ESS)scores compared with the control group in a sensitivity analysis(MD:-1.50,95%CI:-2.13 to-0.88,P<0.00001).CM granules showed a better improvement of ESS than the control group(MD:-1.35,95%CI:-1.92 to-0.78,P<0.00001).Mild adverse reactions occurred only in five patients and disappeared without special treatment.Conclusion:This study showed favorable therapeutic efficacy of CM on OSA.However,in consideration of the low methodological quality of the included RCTs,more rigorous designed,large sample size RCTs are recommended for providing more high-quality evidences.

Analysis on the formation principle and present situation of nano phase state of multi-component self-assembly of traditional Chinese compound medicine decoction

Traditional Chinese medicine decoction is a complex polydispersed phase system containing real solution,colloid solution,emulsion and suspension.The compound decoction of traditional Chinese medicine has complex components,including saponins,alkaloids,polysaccharides,flavonoids,amino acids and so on,which can be self-assembled to form gels,fibers,micelles,vesicles and so on.The self-assembled nano-phase not only neutralizes the single drug and reduces the toxicity and side effects,but also has its own pharmacological effects,which complement each other to achieve synergistic effect,so as to achieve the role of drug supplement,which is of research significance.The formation principle,solubilization and synergism principle and characterization method of multi-component self-assembly of traditional Chinese medicine compound decoction are discussed in this paper.

Review on the progress of treating gastric precancerous lesions with traditional Chinese medicine based on regulatory genes

In recent years,the incidence of Precancerous lesions of gastric cancer(PLGC)has gradually increased,and it is difficult to be cured and easy to recur.Traditional Chinese Medicine(TCM)emphasizes the prevention before the disease.The regulation of PLGC related tumor genes by Chinese medicine has become a research hotspot.This paper summarized PLGC related proto-oncogenes,tumor suppressor genes and apoptosis related genes,and discussed the mechanism of Chinese Medicine Therapy PLGC from the perspective of gene expression,providing new ideas and methods for clinical treatment of PLGC.

Network-meta analysis of 9 kinds of patent Chinese medicine for nourishing qi and activating blood in the treatment of angina pectoris of coronary heart disease

Objective:To evaluate the efficacy and safety of Yiqi Huoxue oral Chinese patent medicine in the treatment of angina pectoris.Methods:the databases of EMBASE,PubMed,CNKI,Wanfang and VIP were searched by computer,and the randomized controlled trial(RCT)of Yiqi Huoxue Chinese patent medicine in the treatment of angina pectoris of coronary heart disease(CHD)was searched until December 2019.By using stata15.1 software,the total clinical effective rate,the improvement rate of ECG,the improvement rate of angina pectoris,the attack frequency and duration of angina pectoris were taken as the outcome indicators for network meta analysis and mapping.Results:51 RCTs were included,including 9 kinds of traditional Chinese medicine.The results of network meta analysis showed that:in terms of clinical total effective rate,the order was Shexiang Baoxin Pill(78.2%)>xueshuanxinming tablet>Dengzhan Shengmai capsule>Shengui Capsule>Tongxinluo capsule>Tongmai Yangxin pill>Yangxin tablet>Shexiang Tongxin drop pill>Naoxintong capsule;the improvement of ECG was Tongmai Yangxin pill(85.2%)>Yangxin tablet>Dengzhan Shengmai capsule>Shexiang Tongxin drop pill>Shexiang Baoxin Pill>Tongxinluo gum Capsule>Shengui Capsule>Naoxintong capsule;in order to reduce the frequency and duration of angina pectoris,they are conventional Naoxintong capsule(88.6%)>Shengui Capsule>Tongmai Yangxin pill>Shexiang Baoxin Pill>xueshuanxinmingpian>Tongxinluo capsule>Shexiang Tongxin drop pill>Dengzhan Shengmai capsule;Shexiang Baoxin Pill(66.9%)>Dengzhan Shengmai capsule>Shexiang Tongxin drop pill>Tongxinluo capsule>xueshuanxinmingning capsule Tablet>Tongmai Yangxin pill.Conclusion:Traditional Chinese medicine combined with western medicine has obvious advantages in the treatment of angina pectoris.Shexiang Baoxin Pill,Dengzhan Shengmai capsule and Tongmai Yangxin pill can be given priority.

Application progress of Chinese medicine in the treatment of irritable bowel syndrome based on the brain-gut axis theory

Irritable bowel syndrome is one of the most common functional gastrointestinal diseases,with a global prevalence of about 12%[1].Modern studies have shown that the abnormality of brain-gut peptides is closely related to the occurrence of irritable bowel syndrome.This article starts with vasoactive intestinal peptide,substance P,serotonin,neuropeptide Y,corticotropin releasing factor,calcitonin gene-related peptide,cholecystokinin and other brain-gut peptides and their correlation with intestinal flora,to summarize the treatment of irritable bowel syndrome with traditional Chinese medicine in recent years.

A meta-analysis of phlegm-resolving Chinese medicine combined with risperidone for treatment of schizophrenia

Objective:The efficacy and occurrence of adverse reactions of prescriptions containing phlegm-resolving Chinese medicine combined with risperidone in the treatment of schizophrenia were retrospectively studied by means of meta-analysis.Methods:A variety of different Chinese and English databases were searched from January 1,2000 to May 1,2020.Review Manager 5.3 software was used to evaluate the methodological quality of the included studies.The positive and negative symptom scale(PANSS)was used as the evaluation standard to analyze the total scores,positive scores,negative scores and general psychopathological scores,clinical efficacy and safety of prescriptions containing phlegmresolving Chinese medicine combined with risperidone of schizophrenia.The heterogeneity test and sensitivity analysis were also carried out by Review Manager 5.3,and the potential publication bias was analyzed by"funnel plot".Results:A total of 1515 patients were included in 15 studies.Compared with control group of risperidone alone,the total PANSS scale score,positive symptom score,negative symptom score and general psychopathological score of the treatment group of phlegm-resolving Chinese medicine combined with risperidone were all lower than those of the control group(P=0.01,MD=-6.35,95%CI[-11.29,-1.40]),(P=0.04,MD=-1.42,95%CI[-2.74,-0.09]),(P<0.01,MD=-2.16,95%CI[-3.41,-0.91]),and(P<0.01,MD=-3.80,95%CI[-6.37,-1.24]),the clinical efficacy of phlegm-resolving Chinese medicine combined with risperidone treatment group on schizophrenia was better than that of the control group[P<0.01,RR=1.09,95%CI(1.05-1.13)].The incidence of adverse reactions was reduced(P=0.02,RR=0.56,95%CI[0.34-0.93]).Conclusion:The combination of prescriptions containing phlegm-resolving Chinese medicine and risperidone can effectively improve the efficacy of patients with schizophrenia and reduce the occurrence of adverse drug reactions compared with risperidone alone.

Research progress of Chinese and Western Medicine on tic disorder in children

Tic disorder is one of the common neuropsychiatric disorders that affect children’s physical and mental health.If it is not tested,diagnosed and treated early,it will have a serious impact on children themselves,their families,and even the whole society.Simple western medicine treatment methods include drugs,nerve regulation,psychological and behavioral therapy,but the results are not ideal.Traditional Chinese medicine takes systematic treatment based on syndrome differentiation and it uses methods of traditional Chinese medicine,massage and acupuncture to regulate five viscera and six bowels in order to harmonized Yin and Yang.These methods make up for the deficiencies of western treatment.In recent years,the clinical treatment of children with Tic disorders involving a variety of combined therapy has achieved significant results.As a result,the rehabilitation of children with Tic disorders has been significantly improved.By consulting the available literature at home and abroad in recent years,the author elaborates the etiology,pathogenesis and treatment of tic disorder in children,in order to further guide the clinical diagnosis and treatment.

A Study of the Treatment of Disfiguring Diseases in the “Qian Jin Fang”

Traditional Chinese medicine(TCM)has been passed down for more than 2,000 years.TCM beauty products driven by the principles of identification and treatment with various dosage forms and exhibit mild and safe efficacy are bound to become a main component of the beauty industry in the future.Xu once commented that“Qian Jin Fang”is different from the traditional prescription system,which emphasizes the use of medicines.In this paper,we mainly selected the prescriptions for the treatment of disfigurement diseases such as acne,scarring,black dryness,face hyperpigmentation,black mole,and rosacea from the article titled,“Upper Seven Orifices Disease-Facial Medicine IX”in the Bei Ji Qian Jin Yao Fang[1].By studying and analyzing the original composition of the formula,understanding the etiology and pathogenesis of the disease,and exploring the characteristics of the formula concerning the efficacy of TCM,their properties,flavors,and meridians,we summarized the characteristics of the Qian Jin Fang.It is based on the external treatment of disfigurement diseases,with a wide variety of drugs,flexibility,and accuracy.This method draws upon analogies while utilizing excipients and harmonizers to focus on the combined healing approach of treatment and nourishment.

Inhibitory effect of acupuncture on neuronalapoptosis in rats after cerebral ischemia

BACKGROUND： Delayed neuronal death after total cerebral ischemia may accompany with apoptosis, but acupuncture may play a certain role in protecting nerve through inhibiting ischemic neuronal apoptosis. OBJECTIVE： To observe the effect of acupuncture on neuronal apoptosis in rats after cerebral ischemia and analyze its cerebral protective mechanism. DESIGN： Contrast observation among groups. SETTING： Heilongjiang University of Traditional Chinese Medicine. MATERIALS： A total of 30 male healthy Wistar rats of general grade and weighing （250±20） g were randomly divided into three groups, including sham operation group, cerebral ischemia group and acupuncture group with 10 rats in each group. Apoptosis in situ kit was provided by Baolingman Company, Germany. METHODS： The experiment was carried out in the Laboratory Center, Heilongjiang University of Traditional Chinese Medicine from May to November 2004. ① Rats in the cerebral ischemia group and the acupuncture group were used to establish total cerebral ischemic models with four vessels occlusion; in addition, models in the sham operation group were established with the same method as mentioned above. However, four vessels of rats in the sham operation were exposured and cerebral ischemia did not occur. Rats in the acupuncture group were given acupuncture treatment after operation. Needle of 40 mm in length was used to acupuncture bilateral Zusanli （St 36） and Quchi （LI 11） with the depth of 3 mm, and then bilateral acupoints were connected with KWD-808Ⅱ omnipotenc impulse electro-therapeutic apparatus （frequency： 1 Hz; thin waves; voltage： 2 V） once a day for totally 30 minutes. Meanwhile, needle of 25 mm in length was used to acupuncture Baihui （Du 20） with the depth of 3 mm, and then the needle was twirled once every 5 minutes for 30 minutes in total. The course was 7 days. ② Neuronal injuries in hippocampal CAI area after cerebral ischemia were observed with Nissl body staining method at 7 days after treatment; neuronal apoptosis was observed with TUNEL staining; manifestations of neuronal apoptosis in cerebral cortex and hippocampal CAI area were observed with electron microscope. MAIN OUTCOME MEASURES： Neuronal injuries in hippocampal CAI area after cerebral ischemia; neuronal apoptosis in cerebral cortex and hippocampal CAI area after cerebral ischemia; morphological changes under electron microscope. RESULTS： Among 30 Wistar rats, 24 rats were involved in the final analysis. ① Expression of positive neurons in cerebral cortex and hippocampal CAI area with Nissl body staining： Neuronal defect was obvious in cerebral cortex and hippocampal CAI area in the cerebral ischemia group as compared with that in the sham operation group （P 〈 0.05）, and neuronal defect was decreased in hippocampal CAI area in the cerebral ischemia group as compared with that in the acupuncture group （P 〈 0.05）. ② Expression of positive neurons in cerebral cortex and hippocampal CAI area with TUNEL staining： Positive neurons with TUNEL staining were not observed in the sham operation group, but positive neurons were increased in the cerebral ischemia group as compared with those in the acupuncture group （P 〈0.05）. ③ Observational results of electron microscope： Neuronal apoptosis was not found in the sham operation group; neuronal apoptosis was rarely found in the acupuncture group; neuronal apoptosis was typical in the cerebral ischemia group. CONCLUSION： Delayed neuronal death after total cerebral ischemia may accompany with apoptosis, but acupuncture may play a certain role in protecting nerve through inhibiting ischemic neuronal apoptosis.

Effect of Acupuncture on Free Radicals in Rats with Early Experimental Spinal Cord Injury

Effect of acupuncture on free radicals after spinal cord injury was observed in rats with experimental spinal cord injury (SCI). Results indicated that within 24 hours after SCI malondialdehyde (MDA) increased progressively, 2 hours after SCI it reached the peak; and the superoxide dismutase (SOD) activity decreased significantly at the same hours, the decrease being the most marked 2-6 hours after SCI. The MDA content in the acupuncture group was significantly lower (P

An Experimental Research into the Anti-aging Effects of Radix Arctii Lappae

Purpose: To delve into the anti-aging effects and mechanism of Niubanggen (牛蒡根Radix Arctii Lappae). Method: The activity of SOD and the content of MDA and lipofuscin in the tissues of the liver,brain and blood serum of the lab rats were observed 30 days after they had been fed with the Niubanggen decoction. Result: The activity of SOD in the liver tissue and blood serum of the decoction-fed lab rats was improved dramatically (P＜0.05 or P＜0.01), the content of MDA in the brain tissue and blood serum lowered obviously (P＜0.05 or P＜0.01), and the content of lipofuscin dropped distinctly (P＜0.01). Conclusion: The mechanism of the anti-aging effects of the Niubanggen is mainly obtained by raising the activity of SOD and reducing the contents of MDA and lipofuscin.

Gentianine protects hippocampal neurons in a rat model of recurrent febrile convulsion

Gentianine has been shown to have a protective effect on hippocampal CA1 neurons in rats subjected to recurrent febrile convulsion（FC）.The present study sought to explore the possible mechanism of gentianine by intraperitoneally injecting gentianine into rats with warm water-induced FC.The results revealed that neuronal organelle injury was slightly ameliorated in the hippocampal CA1 region.The level of glutamate was decreased,but the level of γ-aminobutyric acid was increased,as detected by ninhydrin staining.In addition,glutamate acid decarboxylase expression in hippocampal CA1 was increased,as determined by immunohistochemistry.The results demonstrated that gentianine can ameliorate FC-induced neuronal injury by enhancing glutamate acid decarboxylase activity,decreasing glutamate levels and increasing γ-aminobutyric acid levels.

The research progress of acupuncture and moxibustion in the treatment of peripheral facial paralysis

As a common and frequently-occurring disease in clinic,peripheral facial paralysis is worth paying attention to.In fact,as one of the methods for the treatment of peripheral facial paralysis,acupuncture has been widely promoted and applied in clinic,and has been recognized by doctors at home and abroad.However,there are many other factors that affect the curative effect in clinic due to their different operating methods.Therefore,there are still many disputes in the treatment of peripheral facial paralysis.The relevant literatures in recent years were searched and consulted in order to understand the current situation and provide routine treatment methods for clinical acupuncturists to treat this disease.And we intended to analyze and introduce from acupuncture manipulation,warm acupuncture,electroacupuncture,giant acupuncture,fire acupuncture,acupuncture combined with massage,acupuncture timing and other treatments,and different classification of acupuncture points and methods.

Meta-analysis of the clinical efficacy of Liqi Huoxue drop pill in the treatment of angina pectoris in coronary artery disease

Objective:To evaluate the efficacy of Liqi Huoxue dropping pills in the treatment of angina pectoris in coronary heart disease using the Meta-analysis system.Methods:Randomized controlled trials(RCT)on the treatment of angina pectoris in coronary artery disease with the combination of Western medicine with Liqi Huoxue drop pills were searched using computerized search databases,including English databases PubMed,Embase,Web of Science,The Cochrane Library and Chinese databases CNKI,VIP,WanFang Data and CBM from the time of establishment until February 1,2022.Data extraction was performed independently by two investigators and the quality of the literature of the included studies was assessed.A meta-analysis of the included literature was performed using Revman 5.4 software.Results:Nine studies with a total of 982 patients were included.The results of Meta-analysis showed that the observation group,which adopted conventional treatment of Western medicine combined with Li qi huo xue dropping pills,was better than the control group,which was treated with conventional Western medicine alone.Clinical efficacy of the intervention group[R R=1.20,95%CI=(1.12,1.29),P<0.00001],Angina pectoris symptom treatment[RR=1.17,95%CI=(1.07,1.29),P=0.0010],Number of angina attacks[SMD=-2.26,95%CI(-4.10,-0.42),P=0.002],IL-6[MD=-4.65,95%CI(-6.91,-2.39),P<0.0001],IL-18[MD=-2.53,95%CI(-2.84,-2.22),P<0.00001],HS-CRP[MD=-0.08,95%CI(-1.81,-0.75),P<0.00001],and NO[M D=8.03,95%CI(0.93,15.14),P=0.03]were significantly different and better than the control group(western conventional treatment),and the differences were all statistically significant.Conclusion:Combining Liqi Huoxue drop pill with conventional Western medicine for patients with coronary angina can effectively improve the clinical efficacy,the efficacy of angina symptoms,the number of angina attacks,serum inflammatory indexes interleukin-6,interleukin-18,high-sensitivity C-reactive protein,and endothelial function NO indexes in patients with coronary angina,providing a reliable evidence-based basis for combining Liqi Huoxue drop pill with Western medicine for the treatment of coronary angina.

Meta-analysis of curative effect of Sacubitril valsartan combined with Qiliqiangxin capsule in the treatment of patients with chronic cardiac failure

Objective:To systematically review the effect of Sacubitril valsartan combined with Qiliqiangxincapsule on clinical effect,serological index,cardiac function,quality of live,and adverse reactions in patients with heart failure.Methods:Search the databases of CNKI,VIP,WanFang,CBM,DuXiu,ChiCTR,Web of science,The Cochrane Library,PubMed and Embase to collect the randomized controlled trial(RCT)of Sacubitril valsartan combined with Qiliqiangxin capsule in the treatment of patients with heart failure,The search time limit is from the establishment of the database to May 2021.After the literatures were screened,evaluated and extracted by two researchers independently,Meta analysis was carried out with Stata 16.1 software.Results:A total of 18 RCTs,were included,including 1613 patients.The results of the Meta-analysis showed that there was statistical significance in improving the effective rate(OR=2.60,95%CI[2.09,3.24],P<0.00001),N-terminal pro-brain natriuretic peptide(MD=-468.36,95%CI[-606.80,-329.92],P<0.00001),left ventricular ejection fraction(MD=5.41,95%CI[4.93,5.89],P<0.00001),left ventricular end-diastolic diameter(MD=-3.27,95%CI[-3.65,-2.90],P<0.00001),left ventricular end-systolic diameter(MD=-3.60,95%CI[-4.99,-2.21],P<0.00001),6-minute walking distance(MD=61.42,95%CI[50.04,72.80],P<0.00001),Minnesota living with heart failure questionnaire(MD=-11.39,95%CI[-14.50,-8.28],P<0.00001),and traditional Chinese medicine syndrome score scale(MD=-3.62,95%CI[-6.45,-0.80],P=0.01),but there was no significant difference in cardiac output(MD=0.26,95%CI[-0.02,0.54],P=0.07)and adverse reactions.Conclusion:The current evidence shows that Sacubitril Valsartan combined with Qiliqiangxin capsule can better improve cardiac function,TCM symptoms and quality of life in patients with heart failure than simple Sacubitril Valsartan.However,there was no significantdifference in improving cardiac output between the two groups.However,higher quality RCTs are needed to verify.

Clinical Application of the Point Yongquan

Yongquan (KI 1) is a jing (well) point of the Kidney Meridian of Foot-Shaoyin. As this point is widely used for treatment of acute or chronic diseases, it has drawn attentions from physicians both in the ancient and in the present times. If this point is taken accurately and the differentiation of disease is made properly, both the needling and the external drug application can achieve satisfactory therapeutic results.

Acupuncture Treatment of Habitual Constipation

The authors have in recent years used acupuncture to treat 188 cases of habitual constipation with remarkable curative effects. A report follows.

The effect of Zhusha Anshen Wan decoction on states of the sleep-waking cycle in insomnic rats

BACKGROUND： Sedative and hypnotic chemical drugs prolong the total-sleep time （TST） by a decrease in slow-wave sleep 2 （SWS2） and rapid-eye-movement sleep （REMS） and a relative increase in slow-wave sleep 1 （SWS1）. OBJECTIVE： To investigate the effect of the Chinese medicine Zhusha Anshen Wan at different doses on each sleeping state in insomnic rats, and to identify its mode of action in improving sleep. DESIGN, TIME AND SETTING： A randomized controlled study in rats. This study was performed in the Department of Pharmacology of Chinese Materia Medica, Heilongjiang University of Traditional Chinese Medicine during the period from January 2005 to July 2006. MATERIALS： Twenty-four male Wistar rats, weighing （220±5） g, were selected. The main components in Zhusha Anshen Wan, Cinnabaris, Rhizoma Coptidis, Radix Glycyrrhixae, Prepared Radix Glycyrhizae Radix Angelicae Sinensis, and Rehmannia Pride Rhizome, were authenticated by Dr Xiaowei Du, Professor of Pharmacology. ND-97 Digital Polysomnography was purchased from the Shanghai Medical Instrument High Technology Company and Footplate Electrical Stimulator from the Harbin Research Institute of Electrical Instruments. METHODS： Rats were deprived of sleep by using the Footplate Electrical Stimulator. Insomnic rats were randomized into high-, mid- and low-dose Zhusha Anshen Wan groups, eight rats in each group. Animals were administrated with different doses of Zhusha Anshen Wan （equal to crude drug 7.2, 3.6, 1.8 g/kg） consecutively for seven days. MAIN OUTCOME MEASURES： 30 minutes after the last administration, rats of each group suffered 8 hours foot-shocks while electroencephalography signals were recorded using Digital Polysomnography. Total time of waking （W）, SWS1, SWS2, REMS and TST were calculated for pre- and post-administration, respectively. RESULTS： All 24 rats were included in the statistical analysis of the results without any loss. In the low-dose Zhusha Anshen Wan group, SWS2 was increased significantly compared with pre-administration. In the middle-dose Zhusha Anshen Wan group, W was decreased significantly, but SWS1, SWS2 and TST were increased markedly compared with pre-administration, and there were significant differences between pre- and post-administration （P 〈 0.05-0.01）. In the high-dose Zhusha Anshen Wan group, the duration of W was significantly decreased after administration, but SWS1, SWS2, REMS and TST were significantly longer than pre-administration （P 〈 0.05-0.01）. CONCLUSION： The effect of Zhusha Anshen Wan on sleeping states is dose-dependent. Zhusha Anshen Wan acts by extending SWS1 and SWS2 to increase the total sleeoing time.