The research aimed to study the effects of pine needle on immunity and serum cholesterol of chickens. Totally 240 healthy Roman cocks （one day old） were randomly divided into four groups, three replicates each group...The research aimed to study the effects of pine needle on immunity and serum cholesterol of chickens. Totally 240 healthy Roman cocks （one day old） were randomly divided into four groups, three replicates each group and 20 chickens per replicate. The chickens in contrel group were fed with basal diet and nor- real drinking water; the chickens in groups I and II were fed with the basal diet added with 0.5 and 1.0 g/ehieken pine needle powder, respectively; the chickens in group HI were fed with pine needle decoction at the dose of 0.5 g/chicken. The pre-trial lasted 4 d, and the former trial lasted 70 d. The results showed that the percentages of CD3＋ CD4 ＋ T lymphocytes in groups I, II and III were higher than that in control group at the 15th day after the first immunization, but the percenta- ges in groups 1 and II were lower than that in control group at the 30th , 45th and 60th days, and the percentage in group III was lower than that in control group at the 45th day. The percentages of CD3＋ CD8 ＋ T lymphocytes in groups I, II and III were higher than that in control group at the 15th, 30th and 45th days after the first immunization, but the percentages in groups I and III were lower than that in control group at the 60th day. At the 14th and 21st days after the first immunization, the levels of NDV antibody in groups I and II were lower than that in control group, but the levels at the 28th and 35th days were higher than that in control group. The level of NDV antibody in group III was higher than that in control group at the 14th and 21st days while it was lower at the 7th and 35th days. The levels of serum total cholesterol and trigtyceride in groups I, II and III had lower trend compared with control group, and the levels of serum triglyceride in groups I and II were significantly lower than that in control group and group III （P 〈0.05 ）. The levels of serum low density lipoprotein in groups II and III were lower than that in control group, while the level of serum high density lipoprotein in group II was higher than that in control group, but there were no significant differences （P 〉 0.05 ）. In conclusion, pine needle has many functions as follows ： promoting the proliferation and differentiation of T lymphocytes, improving the content of CD3＋CD4 ＋ and CD3 ＋ CD8＋ lymphocyte, collaborating with vaecine to enhance the specific immune, and reducing the serum cholesterol level in different degrees.展开更多
基金Supported by Major Science and Technology Project in Henan Province(111100110300)Innovative Talents Projects in Universities and Colleges in Henan Province(2012HASTIT008)
文摘The research aimed to study the effects of pine needle on immunity and serum cholesterol of chickens. Totally 240 healthy Roman cocks （one day old） were randomly divided into four groups, three replicates each group and 20 chickens per replicate. The chickens in contrel group were fed with basal diet and nor- real drinking water; the chickens in groups I and II were fed with the basal diet added with 0.5 and 1.0 g/ehieken pine needle powder, respectively; the chickens in group HI were fed with pine needle decoction at the dose of 0.5 g/chicken. The pre-trial lasted 4 d, and the former trial lasted 70 d. The results showed that the percentages of CD3＋ CD4 ＋ T lymphocytes in groups I, II and III were higher than that in control group at the 15th day after the first immunization, but the percenta- ges in groups 1 and II were lower than that in control group at the 30th , 45th and 60th days, and the percentage in group III was lower than that in control group at the 45th day. The percentages of CD3＋ CD8 ＋ T lymphocytes in groups I, II and III were higher than that in control group at the 15th, 30th and 45th days after the first immunization, but the percentages in groups I and III were lower than that in control group at the 60th day. At the 14th and 21st days after the first immunization, the levels of NDV antibody in groups I and II were lower than that in control group, but the levels at the 28th and 35th days were higher than that in control group. The level of NDV antibody in group III was higher than that in control group at the 14th and 21st days while it was lower at the 7th and 35th days. The levels of serum total cholesterol and trigtyceride in groups I, II and III had lower trend compared with control group, and the levels of serum triglyceride in groups I and II were significantly lower than that in control group and group III （P 〈0.05 ）. The levels of serum low density lipoprotein in groups II and III were lower than that in control group, while the level of serum high density lipoprotein in group II was higher than that in control group, but there were no significant differences （P 〉 0.05 ）. In conclusion, pine needle has many functions as follows ： promoting the proliferation and differentiation of T lymphocytes, improving the content of CD3＋CD4 ＋ and CD3 ＋ CD8＋ lymphocyte, collaborating with vaecine to enhance the specific immune, and reducing the serum cholesterol level in different degrees.
