The increasingly frequent exchange of performance data in grid systems across heterogeneous platforms requires a uniform 搑epresentation?of various types of performance data. This paper reviews the current related res...The increasingly frequent exchange of performance data in grid systems across heterogeneous platforms requires a uniform 搑epresentation?of various types of performance data. This paper reviews the current related research, considers the defect of existing methods, and proposes a new portable description method: grid performance data description (GPDD) using an extensible markup language (XML)-based grid performance data representation language (XGPDRL). GPDD describes the abstract structure, which has excellent extensibility (all types of performance data can be described in one format), efficiency, and flexibil-ity. XGPDRL defines the grammar of the GPDD performance data representation, and is both extensible and portable. For benchmarking purposes, performance data can be collected during runtime, represented in XGPDRL, and analyzed visually using a browser across heterogeneous platforms. GPDD and XGPDRL can conveniently ensure data comprehension across various platforms, and are very suitable for grid per-formance data representation.展开更多
Single-atom nanozymes(SANs)possess unique features of maximum atomic utilization and present highly assembled enzyme-like structure and remarkable enzyme-like activity.By introducing SANs into immunoassay,limitations ...Single-atom nanozymes(SANs)possess unique features of maximum atomic utilization and present highly assembled enzyme-like structure and remarkable enzyme-like activity.By introducing SANs into immunoassay,limitations of ELISA such as low stability of horseradish peroxidase(HRP)can be well addressed,thereby improving the performance of the immunoassays.In this work,we have developed novel Fe-N-C single-atom nanozymes(Fe-N_(x)SANs)derived from Fe-doped polypyrrole(PPy)nanotube and substituted the enzymes in ELISA kit for enhancing the detection sensitivity of amyloid beta 1-40.Results indicate that the Fe-N_(x)SANs contain high density of single-atom active sites and comparable enzyme-like properties as HRP,owing to the maximized utilization of Fe atoms and their abundant active sites,which could mimic natural metalloproteases structures.Further designed SAN-linked immunosorbent assay(SAN-LISA)demonstrates the ultralow limit of detection(LOD)of 0.88 pg/mL,much more sensitive than that of commercial ELISA(9.98 pg/mL).The results confirm that the Fe-N_(x)SANs can serve as a satisfactory replacement of enzyme labels,which show great potential as an ultrasensitive colorimetric immunoassay.展开更多
基金the National High-Tech Research and Devel-opment (863) Program of China (No. 2002AA104230)
文摘The increasingly frequent exchange of performance data in grid systems across heterogeneous platforms requires a uniform 搑epresentation?of various types of performance data. This paper reviews the current related research, considers the defect of existing methods, and proposes a new portable description method: grid performance data description (GPDD) using an extensible markup language (XML)-based grid performance data representation language (XGPDRL). GPDD describes the abstract structure, which has excellent extensibility (all types of performance data can be described in one format), efficiency, and flexibil-ity. XGPDRL defines the grammar of the GPDD performance data representation, and is both extensible and portable. For benchmarking purposes, performance data can be collected during runtime, represented in XGPDRL, and analyzed visually using a browser across heterogeneous platforms. GPDD and XGPDRL can conveniently ensure data comprehension across various platforms, and are very suitable for grid per-formance data representation.
基金This work was supported by a start-up fund from Washington State University.
文摘Single-atom nanozymes(SANs)possess unique features of maximum atomic utilization and present highly assembled enzyme-like structure and remarkable enzyme-like activity.By introducing SANs into immunoassay,limitations of ELISA such as low stability of horseradish peroxidase(HRP)can be well addressed,thereby improving the performance of the immunoassays.In this work,we have developed novel Fe-N-C single-atom nanozymes(Fe-N_(x)SANs)derived from Fe-doped polypyrrole(PPy)nanotube and substituted the enzymes in ELISA kit for enhancing the detection sensitivity of amyloid beta 1-40.Results indicate that the Fe-N_(x)SANs contain high density of single-atom active sites and comparable enzyme-like properties as HRP,owing to the maximized utilization of Fe atoms and their abundant active sites,which could mimic natural metalloproteases structures.Further designed SAN-linked immunosorbent assay(SAN-LISA)demonstrates the ultralow limit of detection(LOD)of 0.88 pg/mL,much more sensitive than that of commercial ELISA(9.98 pg/mL).The results confirm that the Fe-N_(x)SANs can serve as a satisfactory replacement of enzyme labels,which show great potential as an ultrasensitive colorimetric immunoassay.
