Objective:To extract,characterize and quantify glycosaminoglycans(GAGs)from the body of cuttlefish,tennis-ball sea cucumber,shrimp,seabass and fresh water fish Nile tilapia.Methods:The extracted crude powder was evalu...Objective:To extract,characterize and quantify glycosaminoglycans(GAGs)from the body of cuttlefish,tennis-ball sea cucumber,shrimp,seabass and fresh water fish Nile tilapia.Methods:The extracted crude powder was evaluated for the content of GAGs.The qualitative analysis of sulfated pattern and other important functional groups related with GAGs were explained in the form of Fourier transform infra-red spectroscopy data.Proteins and nucleic acid in the crude extract were determined by the ultraviolet spectrophotometer,while the quantification of total sulfated GAGs and estimation of N-sulfated and O-sulfated GAGs in the crude mixture were performed by using Blyscan kit.Results:The sulfated pattern and other important functional groups related with GAGs were intercepted in Fourier transform infrared analysis.Blyscan quantification method reported that a rare variety of sea cucumber(tennis-ball sea cucumber)emerged as a rich source of GAGs with high values of both N-sulfated and O-sulfated GAGs in comparison to its other counterparts.Conclusions:Findings in this study point out the potential of tennis-ball sea cucumber,a rare variety of sea cucumber to act as an alternative source for GAG extraction for commercial purpose.展开更多
文摘Objective:To extract,characterize and quantify glycosaminoglycans(GAGs)from the body of cuttlefish,tennis-ball sea cucumber,shrimp,seabass and fresh water fish Nile tilapia.Methods:The extracted crude powder was evaluated for the content of GAGs.The qualitative analysis of sulfated pattern and other important functional groups related with GAGs were explained in the form of Fourier transform infra-red spectroscopy data.Proteins and nucleic acid in the crude extract were determined by the ultraviolet spectrophotometer,while the quantification of total sulfated GAGs and estimation of N-sulfated and O-sulfated GAGs in the crude mixture were performed by using Blyscan kit.Results:The sulfated pattern and other important functional groups related with GAGs were intercepted in Fourier transform infrared analysis.Blyscan quantification method reported that a rare variety of sea cucumber(tennis-ball sea cucumber)emerged as a rich source of GAGs with high values of both N-sulfated and O-sulfated GAGs in comparison to its other counterparts.Conclusions:Findings in this study point out the potential of tennis-ball sea cucumber,a rare variety of sea cucumber to act as an alternative source for GAG extraction for commercial purpose.
