Objectives: This work was carried out in order to determine the profile of different HPV genotypes in a population of women attending radiotherapy (RT). Material and Methods: This is a retrospective study. Sixty paraf...Objectives: This work was carried out in order to determine the profile of different HPV genotypes in a population of women attending radiotherapy (RT). Material and Methods: This is a retrospective study. Sixty paraffin blocks were chosen by simple random method upon 1015 blocks of cervix cancer diagnosed in Senegal’s public hospitals laboratories of anatomopathology. The strains of HPV genotyping were done with polymerase chain reaction (PCR) multiplex using primers MY09-MY11, GP5+ and GP6+. Results: Among the sixty examined blocks, DNA extraction was unsatisfactory in three cases. All women were infected with HPV high risk (HR). The different HPV genotypes isolated were HPV 16 (49/96%), 18 (8/16%), 35 (7/14%), 45 (4/8%), and 58 (2/4%). It was a single infection in 36 cases (70, 60%), co-infection in 11 cases (21, 60%) and a multi-infection in 4 cases (7, 80%). Conclusion: The prevalence of HPV found in our series is comparable to that found in the world. To complete this study, a large population of Senegalese patients with uterine cervix cancer (CC) and HPV genotype, is needed to determine if HPV genotypes could be considered as a prognostic marker in patients with uterine CC after RT.展开更多
文摘Objectives: This work was carried out in order to determine the profile of different HPV genotypes in a population of women attending radiotherapy (RT). Material and Methods: This is a retrospective study. Sixty paraffin blocks were chosen by simple random method upon 1015 blocks of cervix cancer diagnosed in Senegal’s public hospitals laboratories of anatomopathology. The strains of HPV genotyping were done with polymerase chain reaction (PCR) multiplex using primers MY09-MY11, GP5+ and GP6+. Results: Among the sixty examined blocks, DNA extraction was unsatisfactory in three cases. All women were infected with HPV high risk (HR). The different HPV genotypes isolated were HPV 16 (49/96%), 18 (8/16%), 35 (7/14%), 45 (4/8%), and 58 (2/4%). It was a single infection in 36 cases (70, 60%), co-infection in 11 cases (21, 60%) and a multi-infection in 4 cases (7, 80%). Conclusion: The prevalence of HPV found in our series is comparable to that found in the world. To complete this study, a large population of Senegalese patients with uterine cervix cancer (CC) and HPV genotype, is needed to determine if HPV genotypes could be considered as a prognostic marker in patients with uterine CC after RT.
