Objective:Effect analysis of electroacupuncture combined with recircupping in treatment of obese polycystic ovary syndrome.Methods: From October 2016 to September 2018, 68 obese PCOS patients were diagnosed and treate...Objective:Effect analysis of electroacupuncture combined with recircupping in treatment of obese polycystic ovary syndrome.Methods: From October 2016 to September 2018, 68 obese PCOS patients were diagnosed and treated in the department of gynaecology, affiliated hospital of shaanxi university of traditional Chinese medicine.Treatment group given cupping and tired walking tank with metformin therapy, the control group only oral metformin treatment, after continuous treatment of three menstrual cycles, observation comprehensive curative effect of two groups before and after treatment, TCM syndrome score, body weight, body mass index (BMI), waist hip ratio (WHR), sex hormone [luteinizing hormone (LH), testosterone (T)], blood sugar [fasting plasma glucose (FPG), fasting insulin (FINS), insulin resistance index (HOMA IR)] and blood lipid [triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), total cholesterol (TC)] metabolic index, on two groups of curative effect are compared and discussed. Results:The total effective rate (83.33%) of the treatment group was higher than that of the control group (66.67%)(P<0.05).After treatment, TCM syndrome score of the two groups decreased significantly(P<0.01).The treatment group was significantly improved compared with the control group (P<0.01).Body weight, BMI, WHR of the two groups decreased significantly after treatment (P<0.01).The treatment group was better than the control group (P<0.05).After treatment, the LH and T values of the two groups decreased significantly (P<0.01).There was no significant difference in T value between the two groups (P>0.05), but the efficacy of LH in the treatment group was better than that in the control group (P<0.01).The levels of FINS, HOMA-IR and FPG in the two groups were significantly decreased after treatment (P<0.01).There was no significant difference in FPG between the two groups (P > 0.05), but the improvement of FINS and HOMA-IR in the treatment group was better than that in the control group (P< 0.01).After treatment, TG, LDL-C and TC levels in the two groups decreased (P< 0.01), while HDL-C levels in the treatment group increased (P< 0.01).There was no significant difference in HDL-C, LDL-C and TC between the two groups (P>0.05), but the efficacy of TG in the treatment group was better than that in the control group (P< 0.01).Conclusion: The curative effect of electroacupuncture and moving cupping combined with metformin is better than that of metformin alone, should be widely used.展开更多
Objective: This r esea rch showed a method of isolate rat cardio myocytes though acute enzymatic hydrolysis. Method: Our method was divided into five steps: preparation, heart ext raction, perfusion, mechanical dissoc...Objective: This r esea rch showed a method of isolate rat cardio myocytes though acute enzymatic hydrolysis. Method: Our method was divided into five steps: preparation, heart ext raction, perfusion, mechanical dissociation and pu rifica tion and r ecalcifica ti on. Fir stly, make a preparation, including solutions, equipment and so on. Secondly, after anesthesia satisfactorily, open the chest of rat and take the heart out. Thirdly, transect the aorta and hang the aorta onto the Langendroff perfusion system such that the tip is just distal to the aortic valve. For th, perfuse the hea rt with diges tion solution for 17-20 min afte r Ca2+ free tyrode solution for 5 min. Finally, remove the heart from the system quickly and dissociate mechanically in the KB solution and recalcification after filter. Result: Though the microscope, good-state cardiomyocytes (clear stripes, strong sense of three-dimensional, stick well) can be observed. Conclusion: Those cells can be used to undergo medical electrophysiological experiment, including the study of iron channel.展开更多
基金Shaanxi Social development science and Technology project(2016SF-362)
文摘Objective:Effect analysis of electroacupuncture combined with recircupping in treatment of obese polycystic ovary syndrome.Methods: From October 2016 to September 2018, 68 obese PCOS patients were diagnosed and treated in the department of gynaecology, affiliated hospital of shaanxi university of traditional Chinese medicine.Treatment group given cupping and tired walking tank with metformin therapy, the control group only oral metformin treatment, after continuous treatment of three menstrual cycles, observation comprehensive curative effect of two groups before and after treatment, TCM syndrome score, body weight, body mass index (BMI), waist hip ratio (WHR), sex hormone [luteinizing hormone (LH), testosterone (T)], blood sugar [fasting plasma glucose (FPG), fasting insulin (FINS), insulin resistance index (HOMA IR)] and blood lipid [triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), total cholesterol (TC)] metabolic index, on two groups of curative effect are compared and discussed. Results:The total effective rate (83.33%) of the treatment group was higher than that of the control group (66.67%)(P<0.05).After treatment, TCM syndrome score of the two groups decreased significantly(P<0.01).The treatment group was significantly improved compared with the control group (P<0.01).Body weight, BMI, WHR of the two groups decreased significantly after treatment (P<0.01).The treatment group was better than the control group (P<0.05).After treatment, the LH and T values of the two groups decreased significantly (P<0.01).There was no significant difference in T value between the two groups (P>0.05), but the efficacy of LH in the treatment group was better than that in the control group (P<0.01).The levels of FINS, HOMA-IR and FPG in the two groups were significantly decreased after treatment (P<0.01).There was no significant difference in FPG between the two groups (P > 0.05), but the improvement of FINS and HOMA-IR in the treatment group was better than that in the control group (P< 0.01).After treatment, TG, LDL-C and TC levels in the two groups decreased (P< 0.01), while HDL-C levels in the treatment group increased (P< 0.01).There was no significant difference in HDL-C, LDL-C and TC between the two groups (P>0.05), but the efficacy of TG in the treatment group was better than that in the control group (P< 0.01).Conclusion: The curative effect of electroacupuncture and moving cupping combined with metformin is better than that of metformin alone, should be widely used.
文摘Objective: This r esea rch showed a method of isolate rat cardio myocytes though acute enzymatic hydrolysis. Method: Our method was divided into five steps: preparation, heart ext raction, perfusion, mechanical dissociation and pu rifica tion and r ecalcifica ti on. Fir stly, make a preparation, including solutions, equipment and so on. Secondly, after anesthesia satisfactorily, open the chest of rat and take the heart out. Thirdly, transect the aorta and hang the aorta onto the Langendroff perfusion system such that the tip is just distal to the aortic valve. For th, perfuse the hea rt with diges tion solution for 17-20 min afte r Ca2+ free tyrode solution for 5 min. Finally, remove the heart from the system quickly and dissociate mechanically in the KB solution and recalcification after filter. Result: Though the microscope, good-state cardiomyocytes (clear stripes, strong sense of three-dimensional, stick well) can be observed. Conclusion: Those cells can be used to undergo medical electrophysiological experiment, including the study of iron channel.
