BACKGROUND: Hepatic reperfusion injury may cause acute inflammatory damage, producing significant organ dysfunction, and is an important problem in liver transplantation. This experiment aimed to study early changes o...BACKGROUND: Hepatic reperfusion injury may cause acute inflammatory damage, producing significant organ dysfunction, and is an important problem in liver transplantation. This experiment aimed to study early changes of hepatic function after donor liver denervation and Kupffer cell depletion in rat-to-rat liver transplantation and to evaluate the effect of pre-treatment on liver reperfusion injury. METHODS: Donor rats were divided into four groups: control group; group G was pre-treated with gadolinium chloride (G), an inhibitor of Kupffer cells; group H with hexamethonium (H), a sympathetic ganglionic blocking agent; and group HG, with combined H and G pre-treatment. Under the same conditions, serum alanine aminotransferase (ALT), arterial ketone body ratio (AKBR), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and superoxide dismutase (SOD) of recipient rats were assessed at 4, 8, 16 and 24 hours after liver transplantation. Histological studies of the grafts were compared. RESULTS: HG pre-treatment significantly decreased ALT, TNF-alpha, and IL-6 levels, increased AKBR and SOD levels, and demonstrated less pathological damage at 8, 16 and 24 hours compared with the control group. Similar trends were also found in the other groups (G and H). However, the differences among them were not significant at 4 postoperative hours. CONCLUSIONS: Donor denervation and Kupffer cell depletion had preventive effect on liver reperfusion injury. HG pre-treatment is a feasible and reproducible method to protect grafts from reperfusion injury.展开更多
In vivo molecular imaging techniques is increasingly used in the management of oncological patients, allowing different aspects of oncological pathologies to be assessed (e.g. metabolism, hypoxia) non invasively. The ...In vivo molecular imaging techniques is increasingly used in the management of oncological patients, allowing different aspects of oncological pathologies to be assessed (e.g. metabolism, hypoxia) non invasively. The possibility to extract indexes of disease from in vivo biomedical images and to associate them with their biological drivers opens new pro-spective on the role of in vivo molecular imaging and expedites the translation of novel biomarkers from the bench to the clinical environment. In this work we investigate the relationship between 18F-FDG uptake measured by Body- Weight Standardized Uptake Value (SUVBW) as index of cell glucose metabolism, and histological indices for gastric and gastro-oesophageal cancer. For this purpose, Partial Volume Effect Correction (PVC) has been properly compen- sated prior to the measurement of the PET index (PVC-SUVsub>BW). The correlation of 18F-FDG PVC-SUVBW with histol- ogy data was evaluated by bivariate and multivariate statistical analysis. Although obtained in a limited number of pa- tients, our results suggest that correlations can be found when PVC is applied to SUVBW and that 18F-FDG PET can provide information on biological characteristics of gastric and gastro-oesophageal cancer lesions.展开更多
OBJECTIVES: We assessed feasibility of magnetic catheter guidance in patients with atrial fibrillation(AF) undergoing circumferential pulmonary vein ablation( CPVA). BACKGROUND: No data are available on feasibility of...OBJECTIVES: We assessed feasibility of magnetic catheter guidance in patients with atrial fibrillation(AF) undergoing circumferential pulmonary vein ablation( CPVA). BACKGROUND: No data are available on feasibility of remote navigation for AF ablation. METHODS: Forty patients underwent CPVA for symptomatic AF using th e NIOBE II remote magnetic system(Stereotaxis Inc., St. Louis, Missouri). Ablati on was performed with a 4-mm tip, magnetic catheter(65℃, maximum 50 W, 15 s). The catheter tip was guided by a uniform magnetic field(0.08-T), and a motor dr ive(Cardiodrive unit, Stereotaxis Inc.). Left atrium maps were created using an integrated CARTO RMT system(Stereotaxis Inc.). End point of ablation was voltage abatement >90%of bipolar electrogram amplitude. RESULTS: Remote ablation was s uccessful in 38 of 40 patients without complications. The median mapping and abl ation time was 152.5 min(range, 90 to 380 min) but was much longer in the first 12 patients(192.5 min vs. 148 min; p=0.012). Median ablation time was 49.5 min(r ange, 17 to 154 min), but it was much shorter in the last 28 patients than in th e first 12 patients(49 min vs. 70 min; p=0.021). Patients receiving remote ablat ion had longer procedure times than control patients(p< 0.001) with similar mapp ing time but shorter ablation time on right-sided pulmonary veins. Many more ma pping points regardless of their location were collected remotely(p< 0.001). CON CLUSIONS: Remote magnetic navigation for AF ablation is safe and feasible with a short learning curve. Although all pro cedures were performed by a highly experienced operator, remote AF ablation can be performed even by less experienced operators.展开更多
文摘BACKGROUND: Hepatic reperfusion injury may cause acute inflammatory damage, producing significant organ dysfunction, and is an important problem in liver transplantation. This experiment aimed to study early changes of hepatic function after donor liver denervation and Kupffer cell depletion in rat-to-rat liver transplantation and to evaluate the effect of pre-treatment on liver reperfusion injury. METHODS: Donor rats were divided into four groups: control group; group G was pre-treated with gadolinium chloride (G), an inhibitor of Kupffer cells; group H with hexamethonium (H), a sympathetic ganglionic blocking agent; and group HG, with combined H and G pre-treatment. Under the same conditions, serum alanine aminotransferase (ALT), arterial ketone body ratio (AKBR), tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and superoxide dismutase (SOD) of recipient rats were assessed at 4, 8, 16 and 24 hours after liver transplantation. Histological studies of the grafts were compared. RESULTS: HG pre-treatment significantly decreased ALT, TNF-alpha, and IL-6 levels, increased AKBR and SOD levels, and demonstrated less pathological damage at 8, 16 and 24 hours compared with the control group. Similar trends were also found in the other groups (G and H). However, the differences among them were not significant at 4 postoperative hours. CONCLUSIONS: Donor denervation and Kupffer cell depletion had preventive effect on liver reperfusion injury. HG pre-treatment is a feasible and reproducible method to protect grafts from reperfusion injury.
文摘In vivo molecular imaging techniques is increasingly used in the management of oncological patients, allowing different aspects of oncological pathologies to be assessed (e.g. metabolism, hypoxia) non invasively. The possibility to extract indexes of disease from in vivo biomedical images and to associate them with their biological drivers opens new pro-spective on the role of in vivo molecular imaging and expedites the translation of novel biomarkers from the bench to the clinical environment. In this work we investigate the relationship between 18F-FDG uptake measured by Body- Weight Standardized Uptake Value (SUVBW) as index of cell glucose metabolism, and histological indices for gastric and gastro-oesophageal cancer. For this purpose, Partial Volume Effect Correction (PVC) has been properly compen- sated prior to the measurement of the PET index (PVC-SUVsub>BW). The correlation of 18F-FDG PVC-SUVBW with histol- ogy data was evaluated by bivariate and multivariate statistical analysis. Although obtained in a limited number of pa- tients, our results suggest that correlations can be found when PVC is applied to SUVBW and that 18F-FDG PET can provide information on biological characteristics of gastric and gastro-oesophageal cancer lesions.
文摘OBJECTIVES: We assessed feasibility of magnetic catheter guidance in patients with atrial fibrillation(AF) undergoing circumferential pulmonary vein ablation( CPVA). BACKGROUND: No data are available on feasibility of remote navigation for AF ablation. METHODS: Forty patients underwent CPVA for symptomatic AF using th e NIOBE II remote magnetic system(Stereotaxis Inc., St. Louis, Missouri). Ablati on was performed with a 4-mm tip, magnetic catheter(65℃, maximum 50 W, 15 s). The catheter tip was guided by a uniform magnetic field(0.08-T), and a motor dr ive(Cardiodrive unit, Stereotaxis Inc.). Left atrium maps were created using an integrated CARTO RMT system(Stereotaxis Inc.). End point of ablation was voltage abatement >90%of bipolar electrogram amplitude. RESULTS: Remote ablation was s uccessful in 38 of 40 patients without complications. The median mapping and abl ation time was 152.5 min(range, 90 to 380 min) but was much longer in the first 12 patients(192.5 min vs. 148 min; p=0.012). Median ablation time was 49.5 min(r ange, 17 to 154 min), but it was much shorter in the last 28 patients than in th e first 12 patients(49 min vs. 70 min; p=0.021). Patients receiving remote ablat ion had longer procedure times than control patients(p< 0.001) with similar mapp ing time but shorter ablation time on right-sided pulmonary veins. Many more ma pping points regardless of their location were collected remotely(p< 0.001). CON CLUSIONS: Remote magnetic navigation for AF ablation is safe and feasible with a short learning curve. Although all pro cedures were performed by a highly experienced operator, remote AF ablation can be performed even by less experienced operators.
