EFFECTS OF MUTATION AND EXPRESSION OF PTEN GENEmRNA ON TUMORIGENESIS AND PROGRESSION OFEPITHELIAL OVARIAN CANCER

Objective To investigate the mutation and expression of tumor suppressor gene-PTEN mRNA and explore their roles in tumorigenesis and progression of ovarian cancer. Methods Mutated exon 5 of PTEN gene was examined in normal ovary(n = 5), ovarian cyst (n =5), ovarian borderline tumor (n = 9), epithelial ovarian cancer(n = 60), and ovarian cancer cell line (n = 1)by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). mRNA expression of PTEN gene was evaluated in corresponding tissues and cell line by reverse transcription polymerase chain reaction(RT-PCR). The mutation and mRNA expression of PTEN gene were compared with clini-copathological features of ovarian cancer. Results Mutated exon 5 of PTEN gene was detected only in 5(7.1%)cases of epithelial ovarian cancer. mRNA expression level of PTEN gene in ovarian borderline tumor or ovarian cancer was lower than that in normal ovary or ovarian cyst(P < 0.05). The level of PTEN gene mRNA expression was negatively correlated with clinicopathological staging of ovarian cancer, whereas positively correlated with histological differentiation (P < 0.05). mRNA expression level of PTEN gene in ovarian endometrioid cancer was significantly lower than that in ovarian serous or mucinous cancer (P < 0.05=. Conclusions Mutation of PTEN gene occurs in ovarian cancer. Down-regulated expression of PTEN is probably an important molecular event in tumorigenesis of ovarian cancer. Abnormal expression of PTEN gene is involved in progression of ovarian cancer. Reduced expression of PTEN gene is closely associated with tumorigenesis and pathobiological behaviors of ovarian endometrioid cancer.

DIFFERENTIAL EXPRESSION OF ADHESION MOLECULES (CD44,CD29,ICAM-1 AND E-CADHERIN) IN OVARIAN CANCER SK-OV-3ip1 CELLS GROWN AS MONOLAYER AND MULTICELLULAR AGGREGATES

Objective: To detect mRNA levels and expression ofCD44, CD54, CD29 and E-cadherin (E-cad) and to discuss their relationship with formation and drug resistance ofovarian cancer SKOV3ip1 multicellular aggregates.Methods: Liquid overlay system was employed to obtainmulticellular aggregates. mRNA levels and expression ofCD44, CD54, CD29 and E-cad were investigated with RTPCR and flow cytometry (FCM) respectively. Results:Compared with monolayer cells, RT-PCR results showed a decrease in CD44 mRNA level by 0.626-fold and a decrease in CD29 mRNA level by 0.792-fold in multicellularaggregates. However, an increase in CD54 mRNA level by 1.815-fold and an increase in E-cadherin mRNA level by1.344-fold were found in multicellular aggregates. Theresults revealed the downregulation of CD44 and CD29 and the upregulation of CD54 and E-cad genes activity. CD44 expression in monolayer cells and multicellular aggregates were 75.995?.046 and 50.700?.351 (%) respectively andthere was a significant decrease in multicellular aggregates (P=0.001). Compared with control cells, no expression of CD54 was detected in monolayer cells (P=0.563) but markedly elevated CD54 expression was detected in multicellular aggregates (15.780?.217) (%) (P<0.01). High expression of CD29 was seen in monolayer cells and also in multicellular aggregates with positive rates of 96.290+1.201 (%) and 92.494?.055 (%). However, the expression of CD29 in multicellular aggregates was significantly reduced (P=0.014). Also no expression of E-cadherin was found in monolayer cells compared with control cells (4.490?.283) (%) (P=0.65) while significantly increased expression in aggregates cells (17.258?5.572) (%) (P=0.003) was observed. Conclusion: Significant differences in mRNA levels and expression of CD44, CD54, CD29 and E-cadherin clearly exist between monolayer cells and multicellular aggregates, which may be associated with the formation of multicellular aggregates and its drug resistance.

MULTICELLULAR-MEDIATED EXPRESSION OF P-GP AND MRP AND RELATIONSHIP WITH CELL CYCLE PROFILES IN HUMAN OVARIAN CANCER SK-OV-3ip1 MULTICELLULAR AGGREGATES

Objective: To investigate the expression of P-glycoprotein (P-gp) and multidrug resistance-associated protein (MRP) and the relationship with cell cycle profiles in ovarian cancer SK-OV-3ip1 multicellular aggregates. Methods: Liquid overlay system was employed to obtain multicellular aggregates. Expression of P-gp and MRP was detected with flow cytometry (FCM). Outer, intermediate and inner cells from multicellular aggregates were collected by layer-trypsinized method. Cell cycle profiles were also analyzed by FCM. Results: Compared with control cells, no expression of P-gp and MRP was detected in monolyer cells (P=0.128 and P=0.604), but expression of P-gp and MRP in aggregate cells was significantly elevated (P<0.01). P-gp expression in every layer cells was also obviously increased (P<0.01). Furthermore, P-gp expression in every layer cells was also obviously increased (P=0.071). Tendency to increased G0–G1 phase and reduced S phase cells existed from outer through intermediate to inner layers in multicellular aggregates but with no statistical difference. Cell percentages in G2-M phase also had no difference. However, compared with monolayer cells, cells in G0–G1 phase increased and cells in S and G2-M phases lowered significantly in every layer and in the whole multicellular aggregates. Expression elevation of P-gp and MRP was consistent with increased G0–G1 percentage in aggregate cells. Conclusion: Expression of P-gp and MRP increases in cells of SK-OV-3ip1 multicellular aggregates and is consistent with increased G0–G1 percentage, which implies the possible relationship between them and the possible role in multicellular-mediated drug resistance.

Intrauterine Exposure to Maternal Diabetes is Associated with Adiposity in Children at 6 Years of Age in China

Children born to mothers with gestational diabetes mellitus （GDM） are more likely to exhibit congenital malformations, high birth weight, and obesity and have an increased risk of developing type 2 diabetes in later life. Children who are exposed to maternal diabetes in utero may be ＇programmed＇ for later development of obesity at a critical period of development. Therefore, the purpose of the present study was to examine the associations among adiposity and systolic blood pressure in children and abnormal maternal glucose levels during pregnancy. A total of 856 mother-child pairs were included in the present retrospective study.

Quality of Sleep among Pregnant Women

Objectives: The study aimed to describe the quality of sleep and explore factors especially Sleep Hygiene Practices associated with sleep quality among pregnant women. Study design: This is a cross-sectional study. Methods: 119 healthy pregnant women in two central hospitals in Vietnam were participated in this study. The Pittsburgh Sleep Quality Index (PSQI) was employed to evaluate sleep quality and the Modified Sleep Hygiene Practices was used to describe sleeping practices among pregnant women. Results: The results showed that while 58.8% pregnant women had quite good sleep quality, 41.2% of pregnant women had mild to moderate quality sleep disturbance. The quality of sleep was better in second trimester, but then getting worst in the third trimester. Multipara experienced worse sleep quality than nullipara. Poor sleep quality had a positive correlation with unhealthy Sleep Hygiene Practices including using an uncomfortable bed (p < 0.05), going to bed with variable bedtime (p < 0.01), watching TV or making call in bed other than sleep (p < 0.05), going to sleep without sleep sensation (p < 0.001) and staying in bed even though it was difficult to fall asleep (p < 0.001). Conclusion: Pregnant women should be taken care of on sleep quality, especially in the first and third trimester as they experience more change in hormone and physiology. Sleep Hygiene Practices should be emphasized in health education for pregnant women.

MULTICELLULAR-MEDIATED RESISTANCE TO CISPLATIN AND TAXOL IN HUMAN OVARIAN CANCER SK-OV-3IP1 MULTICELLULAR AGGREGATES

Objective: To investigate the chemosensitivity of ovarian cancer SK-OV-3ip1 multicellular aggregates (MCA) to cisplatin and taxol and to explore the possible mechanisms. Methods: Liquid overlay system was employed to obtain MCA. We detected the resistance using trypan blue exclusion testing, clonogenic assay, cell cycle profiles and apoptosis with flow cytometry (FCM). Results: After cisplatin exposure, MCA cells showed nearly equal cell viability with monolayer cells (P=0.05). After 40μM cisplatin exposure for 12 h, no clone (≥50 cells) was formed, but more viable cells attached to the bottom of 24-well plate in MCA group than monolayer. Furthermore, apoptosis rate and cell cycle profiles with FCM had no significant change between MCA and monolayer cells. After taxol exposure, however, trypan blue exclusion testing demonstrated higher cell viability in MCA cells (P=0.003) and higher clone formation rate in 100-cell group than monolayer cells (0.01<P<0.025). No significant difference was found in 50-cell or 200-cell group but more viable cells in MCA group were observed. Taxol exposure caused significantly decreased apoptosis rate in MCA cells than monolayer cells (P=0.012). Taxol induced significant cell arrest at G2-M phase in monolayer cells (P=0.001), but abrogation of G2-M arrest was observed in MCA cells (P=0.002). Conclusion: Compared with monolayer cells, MCA cells from the same SK-OV-3ip1 cell line appear to be more resistant to taxol but not to cisplatin. Cell cycle redistribution and multicellular-mediated inhibition of apoptosis can partially account for the resistance.

Microperforate (Pinhole) hymen and infertility;a rare case report

Inroduction Female genital tract anomalies may have a devastating impact on the potential for sexual activity and fertility. Microperforate (pinhole) hymen is one of them. Microperforate hymen can lead to infertility, primary amenorrhea, acute or chronic pelvic pain, abnormal vaginal bleeding, dysuria, pollakiuria or a foul-smelling vaginal discharge. Aim We aim to report a patient who had intercourse problems and infertility complaints later found to have a microper-forate hymen. Discussion Pinhole opening of hymen may permit passage of fluids. Consequently spontaneous pregnancy can occur rarely. A few reports showed pregnancies with intact hymen without penetration of penis. But in general patients have fertility problems. Conclusion The results of the surgery are similar in all techniques and the particular surgical centre will have its own preference of which technique it prefers since after diagnosis definitive treatment is surgical and effective. Failure to manage these patients correctly may have long-term sequelae for their psychological, sexual and reproductive health.

A review of HELLP syndrome, in 17 patients

Introduction Preeclampsia is a well known obstetric complication characterized with hypertension and proteinuria whereas HELLP syndrome (hemolysis, elevated liver enzymes, low platelet count) which was first stated by Prichard in 1954 and described by Weinstein in 1982, is a multisystemic disease usually seen in the third trimester and in the postpartum period, usually in 48 - 72 hours following delivery [1]. Aim In this study we aim to share our experience in 17 patients with HELLP syndrome treated in our clinic between January 2005 and March 2008 in regard of the current literature. Material and Methods 17 patients diagnosed with HELLP syndrome between January 2005 and March 2008 were retro-spectively re-evaluated in regard of symptoms, treatment options and prognosis. As such this is a retro-spective study focusing on case reports. Conclusion As noted before main focus of treatment should be on patient stability, in other words, palliative care under intensive care conditions.

Nutritional status of Vietnamese infants assessed by Fenton growth chart and related factors:A cross-sectional study

Importance:Nutritional status of infants,measured by birth weight and length,is an essential factor in neonatal development.Malnutrition in newborns may lead to a higher risk of mortality,neurological and cognitive impairment,and poor language development.Objective:This study aims to assess the nutritional status of infants and related factors regarding maternal anthropometric characteristics and medical history.Methods:A cross-sectional study was conducted at the National Hospital of Obstetrics and Gynecology,Vietnam from May 2021 to May 2022 on 340 infants and mothers.Low birth weight was defined following the Intergrowth-21 standards.Stunting was evaluated using the Fenton growth chart when the length was below the 10%percentile line of the gestational week.Multivariate regression models were applied to identify factors associated with the nutritional status of infants.Results:We found that 12.4%and 14.1%of infants in our study fell into stunted and underweight categories,respectively.Infants of mothers over 35 years old,having a height lower than 150 cm or experiencing anemia during pregnancy were more likely to be stunted or have low birth weight.Serum albumin deficiency during pregnancy was strongly associated with the infant being underweight(odds ratio[OR]=2.8,95%confidence interval[95%CI]1.1–7.3).Newborns were more likely to be stunted if their mothers had a history of preterm birth(OR=3.3,95%CI 1.1–10.2).Interpretation:Maternal nutritional status is closely related to infant malnutrition,particularly in preterm infants.Improving the understanding of mothers regarding prenatal care,reproductive healthcare,adequate nutritional diet,and multi-micronutrient supplements during pregnancy is therefore important.

Expression of Key Steroidogenic Enzymes in Human Placenta and Associated Adverse Pregnancy Outcomes

Steroid hormones,including progestagens,estrogens,androgens,corticosteroids,and their precursor cholesterol,perform essential functions in the successful establishment and maintenance of pregnancy and normal fetal development.As the core endocrine organ at the prenatal stage,the human placenta is involved in the biosynthesis,metabolism,and delivery of steroid hormones.Steroidogenic pathways are tightly regulated by placenta-intrinsic cytochrome P450 and hydroxysteroid dehydrogenase.However,the relationship between placental steroidogenic enzyme expression and adverse pregnancy outcomes is controversial.In this review,we summarize the possible upstream regulatory mechanisms of placental steroidogenic enzymes in physiologic and pathophysiologic states.We also describe the human placental barrier model and examine the potential of single-cell sequencing for evaluating the primary functions and cellular origin of steroidogenic enzymes.Finally,we examine the existing evidence for the association between placental steroidogenic enzyme dysregulation and adverse pregnancy outcomes.

Efficacy of intrauterine balloon stent or oral estrogen on prevention of adhesion after transcervical resection of septum in septate uterus:Study protocol for a randomized controlled multicenter study in China

To the Editor:The septate uterus is the most common uterine anomaly and is associated with an increased risk of adverse reproductive outcomes.Transcervical resection of septum(TCRS)is widely performed in patients with adverse reproductive outcomes.The incidence of de novo intrauterine adhesions(IUAs)after TCRS is approximately 5%to 25%.[1]Various anti-adhesion methods are widely used in the clinical management of patients with septate uteri,including intrauterine devices,Foley balloons,estrogen,antibiotics,and hyaluronic acid gel.However,insufficient evidence supports or opposes any of these methods(Level C),[2]lacking high-grade evidence.

Intrauterine Treatment of Monochorionic Triamniotic Triplet Pregnancy with Twin Reverse Arterial Perfusion Sequence

To editor:This study reports a pregnant woman who was naturally conceived with monochorionic triamniotic(MCTA)combined with twin reversed arterial perfusion(TRAP).An ultrasound revealed that one of the fetuses had a cardiac malformation,and a connection between the umbilical arteries was suspected.To reduce the acardiac fetus,radiofrequency ablation was performed at 17+3 weeks of pregnancy.At 36+4 weeks,two healthy infants were delivered via elective cesarean section.Postoperative examination revealed a direct connection between the umbilical arteries,with the placental perfusion acting as a direct pump.It is essential to identify the intrauterine status of the blood-supplying infant and vascular connections in order to achieve successful outcomes in pregnancies with complications,such as edema and heart failure.The studies involving human participants were reviewed and approved by Human Research Ethics Committee of Tianjin Central Hospital of Obstetrics and Gynecology.The patient provided her written informed consent to participate in this study.

Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers

Cervical cancer is the leading cause of death among women with cancer worldwide.Here,we performed an integrative analysis of Illumina HumanMethylation450K and RNA-seq data from TCGA to identify cervical cancer-specific DNA methylation markers.We first identified differentially methylated and expressed genes and examined the correlation between DNA methylation and gene expression.The DNA methylation profiles of 12 types of cancers,including cervical cancer,were used to generate a candidate set,and machine-learning techniques were adopted to define the final cervical cancer-specific markers in the candidate set.Then,we assessed the protein levels of marker genes by immunohistochemistry by using tissue arrays containing 93 human cervical squamous cell carcinoma samples and cancer-adjacent normal tissues.Promoter methylation was negatively correlated with the local regulation of gene expression.In the distant regulation of gene expression,the methylation of hypermethylated genes was more likely to be negatively correlated with gene expression,while the methylation of hypomethylated genes was more likely to be positively correlated with gene expression.Moreover,we identified four cervical cancer-specific methylation markers,cg07211381(RAB3C),cg12205729(GABRA2),cg20708961(ZNF257),and cg26490054(SLC5A8),with 96.2%sensitivity and 95.2%specificity by using the tenfold cross-validation of TCGA data.The four markers could distinguish tumors from normal tissues with a 94.2,100,100,and 100%AUC in four independent validation sets from the GEO database.Overall,our study demonstrates the potential use of methylation markers in cervical cancer diagnosis and may boost the development of new epigenetic therapies.

Effects of Ziyin Jianghuo Ningxin decoction plus dehydroepiandrosterone and femoston in treatment of patients with menopausal symptoms

OBJECTIVE: To determine the therapeutic effect of Ziyin Jianghuo Ningxin Decoction(ZYJHNXD) plus dehydroepiandrosterone(DHEA) and menopausalhormone therapy(MHT) in patients suffering from menopausal symptoms identified as, in terms of Traditional Chinese Medicine, symptom pattern of Yin deficiency with hyperactive fire.METHODS: Totally 180 postmenopausal women aged 40 to 60 years were assigned into four groups and accepted femoston, femoston with ZYJHNXD,femoston with DHEA, femoston with ZYJHNXD and DHEA therapies, respectively, for three months.Common questionnaire-based measure instruments included modified Kupperman index(MKI),Hamilton Rating Scale for Anxiety(HAMA), and Hamilton Rating Scale for Depression(HAMD). Follicle-stimulating hormone(FSH), luteinizing hormone(LH), estradiol(E2), 5-hydroxyindole-3-acetic acid(5-HIAA), norepinephrine(NE), dopamine(DA),bone mineral density(BMD), and sleep quality were evaluated before and three months after the treatments.RESULTS: In all four groups, the scores of MKI, HAMA, HAMD and the levels of FSH, LH decreased significantly(P < 0.05) after the treatment, while the levels of E2, 5-HIAA, NE, and DA showed obvious elevation(P < 0.05). The group receiving ZYJHNXD and DHEA combined with femoston had superiority in the preservation of bone mineral density and improvement of total sleep time and nighttime sleep time over the other three groups.CONCLUSION: ZYJHNXD and DHEA combined with MHT therapy have a favorable outcome in managing menopausal symptoms, restoring hormone levels, preventing skeletal rarefaction or osteoporosis,and improving sleep quality for postmenopausal women.

Novel MLH1 frameshift mutation in an extended hereditary nonpolyposis colorectal cancer family

AIM: To present novel frameshift mutation c.31delC [p.L11X] in the MLH1 gene identified in an extended Bulgarian hereditary non-polyposis colorectal cancer (HNPCC) family and to analyze the molecular and clinical findings within the pedigree concerning the proposal of adequate individual prophylactic strategy for all mutation carriers. METHODS: The pedigree of the family consists of 42 members in four generations. Search for mutations in the MLH1 and hMSH2 genes was performed in the pro-band. After PCR amplification of all exons including flanking intronic regions, amplicons were directly sequenced. RESULTS: The mutation was found in nine from the thirteen pedigree members who signed informed consent to participate in the study. In three adenocarcinomas, microsatellite instability and lack of the MLH1 protein expression were detected. The only one tubulovillous adenoma analyzed was microsatellite stable and the MLH1 protein showed an intact staining. CONCLUSION: The newly described mutation c.31delC is HNPCC causative. Besides the typical clinical features of the syndrome, we found a specific pathologic manifestation such as moderate to high differentiated adenocarcinomas of the colon. One of the mutation carriers developed a benign giant cell soft tissue tumor. The primary tumor localizations were frequently extracolonic and detailed yearly gastrointestinal and gynecological examinations have been proposed to the mutation carriers. We emphasize the importance of including the HNPCC genetic counseling and testing as well in the following surveillance of all patients at risk in the services covered by the health insurance in Bulgaria.

Use of antenatal corticosteroids among infants with gestational age at 24 to 31 weeks in 57 neonatal intensive care units of China: a cross-sectional study

Background:Antenatal corticosteroids(ACS)can significantly improve the outcomes of preterm infants.This study aimed to describe the ACS use rates among preterm infants admitted to Chinese neonatal intensive care units(NICU)and to explore perinatal factors associated with ACS use,using the largest contemporary cohort of very preterm infants in China.Methods:This cross-sectional study enrolled all infants born at 24^(+0)to 31^(+6)weeks and admitted to 57 NICUs of the Chinese Neonatal Network from January 1st,2019 to December 30th,2019.The ACS administration was defined as at least one dose of dexamethasone and betamethasone given before delivery.Multiple logistic regressions were applied to determine the association between perinatal factors and ACS usage.Results:A total of 7828 infants were enrolled,among which 6103(78.0%)infants received ACS.ACS use rates increased with increasing gestational age(GA),from 177/259(68.3%)at 24 to 25 weeks’gestation to 3120/3960(78.8%)at 30 to 31 weeks’gestation.Among infants exposed to ACS,2999 of 6103(49.1%)infants received a single complete course,and 33.4%(2039/6103)infants received a partial course.ACS use rates varied from 30.2%to 100%among different hospitals.Multivariate regression showed that increasing GA,born in hospital(inborn),increasing maternal age,maternal hypertension and premature rupture of membranes were associated with higher likelihood to receive ACS.Conclusions:The use rate of ACS remained low for infants at 24 to 31 weeks’gestation admitted to Chinese NICUs,with fewer infants receiving a complete course.The use rates varied significantly among different hospitals.Efforts are urgently needed to propose improvement measures and thus improve the usage of ACS.

Aspirin Enhances the Protective Effect of Progesterone on Trophoblast Cell from Oxidative Stress and Apoptosis

Objective:Clinically,low-dose aspirin and progesterone are frequently used to prevent pregnancy loss.We investigated the effect of these drugs on the biological behavior of human extravillous trophoblasts in vitro.Methods:HTR-8/SVneo cells were cultured in vitro and treated with different concentrations of aspirin and progesterone.The proliferation,invasion,and apoptosis of HTR-8/SVneo cells were assessed using a cell counting Kit-8 assay,Matrigel Transwell assay,and Hoechst staining,respectively.Reverse transcriptase polymerase chain reaction was used to verify the expression of related genes.Reactive oxygen species(ROS)levels were detected using the 2,7-dichlorofluorescin diacetate assay.Results:Low-dose aspirin alone,progesterone alone,or aspirin plus progesterone upregulated the proliferation and invasion and decreased the apoptosis of HTR-8/SVneo cells.Moreover,the expression of marker of proliferation Ki-67(MKI67),matrix metalloproteinases 2(MMP2),and MMP9 was increased.In addition,low-dose aspirin plus progesterone exerted stronger anti-apoptosis effects than low-dose aspirin and progesterone alone.Interestingly,aspirin upregulated the expression of progesterone receptor(PGR).Treatment with hydrogen peroxide(H_(2)O_(2))promoted ROS production in HTR-8/SVneo cells;however,low-dose aspirin plus progesterone significantly restricted H_(2)O_(2)-mediated ROS production and apoptosis in HTR-8/SVneo cells.Conclusions:These data suggest that low-dose aspirin and progesterone promote proliferation and invasion and cooperatively reduce oxidative stress and apoptosis in trophoblasts in vitro.These results may provide an experimental basis for the combined application of aspirin and progesterone to prevent unexplained recurrent spontaneous miscarriage,especially in patients with trophoblast dysfunction.

Interaction between Kynurenine and Aryl Hydrocarbon Receptor in Regulating the Balance of T helper 17 Cells and Regulatory T-cells in Decidua during Early Gestation

Objective:To investigate whether kynurenine/aryl hydrocarbon receptor(AHR)affects the maternal-fetal tolerance by involving the differentiation of T helper 17(Th17)/regulatory T(Treg)cells,and to provide theoretical basic for new treatment of unexplained abortion.Methods:Flow cytometry(FCM)was used to detect the expression of AHR in peripheral/decidual CD4+T,Treg,and Th17 cells.The effect of Kyn on the differentiation of peripheral/decidual na?ve T-cells under Treg-/Th17-polarizing condition was detected by FCM;enzyme-linked immunosorbent assay was performed to examine the level of Kyn in villus and decidual tissues from normal pregnancy(NP)and unexplained abortion(UA).Student’s t-test in the case of two groups or one-way ANOVA in multiple groups was used.Results:AHR expression in CD4+T-cells was decreased in decidua versus blood in early pregnancy(P<0.0001).Kyn could promote the differentiation of peripheral and decidual na?ve T-cells to Th17 cells under Treg-polarizing conditions(P<0.01).There was no statistical significance about the concentration of Kyn in decidual or villi tissues between NP and UA,and compared with NP,the expression of AHR in decidual CD4+T-cells from UA was increased(P<0.001).Conclusions:Kyn/AHR promotes Th17 and restricts Treg cells’differentiation,which is involved in maintaining the balance of Treg/Th17 cells at the maternal-fetal interface.

卵巢上皮性癌和血小板计数增高相关性分析(英文)

Objective:To evaluate the clinical significance of platelet(PLT) count in epithelial ovarian cancer,and to inves-tigate the correlation between thrombocytosis and the incidence of epithelial ovarian cancer.Methods:We evaluated 220 epithelial ovarian tumor patients divided into early stage epithelial ovarian cancer group(n = 80),advanced stage epithelial ovarian cancer group(n = 50) and benign ovarian tumor group(n = 90) as controls,who underwent primary surgical treatment.Three groups were evaluated with the relationship between platelet counts and preoperative and postoperative CA125,histo-pathology,abdominal edema,residual tumor,and lymph node metastasis.Epithelial ovarian cancer patients were evaluated whether platelet count was decreased after surgery.Results:The mean platelet counts were(234.55 ± 71.51) x 109/L in the early stage epithelial ovarian cancer group,(308.12 ± 111.95) x 109/L in the advanced stage epithelial ovarian cancer group,and(206.28 ± 52.62) x 109/L in the benign ovarian tumor group,with a significant difference among the 3 groups(P < 0.05).In the early stage epithelial ovarian cancer group,the platelet count was correlated with histopathology.In the advanced stage epithelial ovarian cancer group,there was a correlation between thrombocytosis and the incidence of that residual tumor diameter was greater than 2 cm.But there was no relationship between platelet count and histopathology,CA125,abdominal edema,or lymph node metastasis.In general the platelet count was decreased after surgery.Conclusion:An increased platelet count is commonly seen in patients with epithelial ovarian cancer,but it usually decreases after surgery.Patients with thrombocytosis have poor prognosis.Platelet count can be used as a marker for the development and prognosis of epithelial ovarian cancer.