Effect of zhenwu decoction combined with neoadjuvant chemotherapy on malignant characteristics of cancer cells in ovarian cancer

Objective:To explore the effect of zhenwu decoction combined with neoadjuvant chemotherapy on malignant characteristics of cancer cells in ovarian cancer.Methods:A total of 80 patients with ovarian cancer who underwent surgical treatment in this hospital between March 2015 and January 2017 were divided into the control group (n=42) who received preoperative neoadjuvant chemotherapy and the zhenwu decoction group (n=38) who received preoperative zhenwu decoction combined with neoadjuvant chemotherapy. Intraoperative ovarian cancer tissue was kept to detect the expression of proliferation genes, invasion genes and autophagy genes through fluorescence quantitative PCR method.Results:Proliferation genes AKT, LSD1, SIRT1 and NOB1 mRNA expression in ovarian lesion tissue of zhenwu decoction group were lower than those of control group;invasion gene DACT1 mRNA expression was higher than that of control group whereas MTA1 and GRP78 mRNA expression were lower than those of control group;autophagy gene Beclin1 mRNA expression was higher than that of control group whereas LC3-Ⅱ and Atg5 mRNA expression were lower than those of control group.Conclusion:Preoperative zhenwu decoction combined with neoadjuvant chemotherapy can effectively inhibit the proliferation and invasion activity of tumor cells and regulate their autophagy function in patients with ovarian cancer.

Analyses and identification of the chemical constituents of the Uighur formulation Baixuan Xiatare using three-stage infrared spectroscopy

OBJECTIVE:To identify,rapidly and accurately,the chemical composition of the traditional Uighur formulation Baixuan Xiatare(BXXTR-FU).METHODS:We investigated if application of three-stage infrared(IR)spectroscopy enabled identification of the main chemical constituents(and their origins)in BXXTR-FU.RESULTS:The characteristic peaks of herbal material(s)and BXXTR-FU were assigned.In Fourier transform-IR(FT-IR)spectroscopy of BXXTR-FU,peaks at1616 and 1605 cm-1 of BXXTR-FU were considered to denote anthraquinones and their derivatives;1066 cm-1 was regarded as the characteristic absorption peak of resin glycosides.In second-derivative IR(SD-IR)spectroscopy,the main carbonyl types of BXXTR-FU in the range 1743-1636 cm-1 were assigned:1651 cm-1 belonged to the carbonyl stretching vibrations of flavonoids and chromones;1717 cm-1 belonged to tannins;1699 cm-1 belonged to carboxylic acids;1636 cm-1 belonged to anthraquinones and their derivatives.SD-IR spectroscopy further confirmed that the characteristicabsorption peaks at 1636,1618 and 1603 cm-1 could be used as markers that BXXTR-FU contained anthraquinones and their derivatives.Synchronous2 D-IR correlation spectra of chemical groups further confirmed the results of FT-IR and SD-IR spectroscopy.CONCLUSION:Our study strongly supported the necessity and importance of three-stage IR spectroscopy owing to its rapid and accurate identification of herbal formulations.