Cyclophilin(CYP)plays an important role in plant response to stress,and OsCYP2,one gene of cyclophlilin family,is involved in auxin signal transduction and stress signaling in rice.However,the mechanism that OsCYP2 is...Cyclophilin(CYP)plays an important role in plant response to stress,and OsCYP2,one gene of cyclophlilin family,is involved in auxin signal transduction and stress signaling in rice.However,the mechanism that OsCYP2 is involved in rice response to low temperature is still unclear.We identified a new OsCYP2 allelic mutant,lrl3,with fewer lateral roots,and the differences in shoot height,primary root length and adventitious root length increased with the growth process compared to the wild-type plant.Auxin signaling pathway was also affected and became insensitive to gravity.The transgenic rice plants with over-expression of OsCYP2 were more tolerant to low temperature than the wild-type plants,suggesting that OsCYP2 was involved in the low temperature response in rice.In addition,OsCYP2 negatively regulated the expression of OsTPS38,a terpene synthase gene,and was dependent on the OsCDPK7-mediated pathway in response to low temperature stress.OsTPS38-overexpressed transgenic line ox-2 was more sensitive to low temperature.Therefore,OsCYP2 may negatively regulate OsTPS38 through an OsCDPK7-dependent pathway to mediate the response to low temperature in rice.These results provide a new basis for auxin signaling genes to regulate rice response to low temperature stress.展开更多
基金The datasets presented in this study can be found in online repositories.The names of the repository/repositories and accession number(s)can be found below:NCBI-SRA database under the BioProject no.PRJNA732107 and accession nos.SRR14629497,SRR14629496,SRR14629495,and SRR14629494 for the RNA-seq data.
文摘Cyclophilin(CYP)plays an important role in plant response to stress,and OsCYP2,one gene of cyclophlilin family,is involved in auxin signal transduction and stress signaling in rice.However,the mechanism that OsCYP2 is involved in rice response to low temperature is still unclear.We identified a new OsCYP2 allelic mutant,lrl3,with fewer lateral roots,and the differences in shoot height,primary root length and adventitious root length increased with the growth process compared to the wild-type plant.Auxin signaling pathway was also affected and became insensitive to gravity.The transgenic rice plants with over-expression of OsCYP2 were more tolerant to low temperature than the wild-type plants,suggesting that OsCYP2 was involved in the low temperature response in rice.In addition,OsCYP2 negatively regulated the expression of OsTPS38,a terpene synthase gene,and was dependent on the OsCDPK7-mediated pathway in response to low temperature stress.OsTPS38-overexpressed transgenic line ox-2 was more sensitive to low temperature.Therefore,OsCYP2 may negatively regulate OsTPS38 through an OsCDPK7-dependent pathway to mediate the response to low temperature in rice.These results provide a new basis for auxin signaling genes to regulate rice response to low temperature stress.
