Smart Farming for Sustainable Rice Production:An Insight into Application,Challenge,and Future Prospect

Rice has a huge impact on socio-economic growth,and ensuring its sustainability and optimal utilization is vital.This review provides an insight into the role of smart farming in enhancing rice productivity.The applications of smart farming in rice production including yield estimation,smart irrigation systems,monitoring disease and growth,and predicting rice quality and classifications are highlighted.The challenges of smart farming in sustainable rice production to enhance the understanding of researchers,policymakers,and stakeholders are discussed.Numerous efforts have been exerted to combat the issues in rice production in order to promote rice sector development.The effective implementation of smart farming in rice production has been facilitated by various technical advancements,particularly the integration of the Internet of Things and artificial intelligence.The future prospects of smart farming in transforming existing rice production practices are also elucidated.Through the utilization of smart farming,the rice industry can attain sustainable and resilient production systems that could mitigate environmental impact and safeguard food security.Thus,the rice industry holds a bright future in transforming current rice production practices into a new outlook in rice smart farming development.

Ethanol extract of Kalopanax septemlobus leaf inhibits HepG2 human hepatocellular carcinoma cell proliferation via inducing cell cycle arrest at G_1 phase

Objective:To investigate the effects of an ethanol extract of Kalopanax septemlobus(Thunb.)Koidz.leaf(EEKS) on cell proliferation in human hepatocellular carcinoma cells and its mechanisms of action.Methods:Cells were treated with EEKS and subsequently analyzed for cell proliferation and flow cytometry analysis.Expressions of cell cycle regulators were determined by reverse transcriptase polymerase chain reaction analysis and Western blotting,and activation of eyclin-associaled kinases studied using kinase assays.Results:The EEKS suppressed cell proliferation in both HepG2 and Hep3 B cells,but showed a more sensitive anli-proliferative activity in HepG2 cells.Flow cytometry analysis revealed an association between the growth inhibitory effect of EEKS and with G_1 phase cell cycle arrest in HepG2 cells,along with the dephosphorylation of retinoblastoma protein(pRB) and enhanced binding of pRB with the E2 F transcription factor family proteins.Treatment with EEKS also increased the expression of cyclin-dependent kinase(CDK) inhibitors,such as p21WAF1/CIP1 and p27KIP1.without any noticeable changes in G_1 cyclins and CDKs(except for a slight decrease in CDK4).Treatment of HepG2 cells with EEKS also increased the binding of p21 and p27 with CDK4 and CDK6.which was paralleled by a marked decrease in the cyclin D- and cyclin E-associated kinase activities.Conclusions:Overall,our findings suggest that EEKS may be an effective treatment for liver cancer through suppression of cancer cell proliferation via G_1,cell cycle arrest Further studies arc required to identify the active compounds in EEKS.

Quercetin exerts anti-inflammatory effects via inhibiting tumor necrosis factor-α-induced matrix metalloproteinase-9 expression in normal human gastric epithelial cells

BACKGROUND Gastric injury is the most common digestive system disease worldwide and involves inflammation,which can lead to gastric ulcer or gastric cancer(GC).Matrix metallopeptidase-9[MMP-9(gelatinase-B)]plays an important role in inflammation and GC progression.Quercetin and quercetin-rich diets represent potential food supplements and a source of medications for treating gastric injury given their anti-inflammatory activities.However,the effects and mechanisms of action of quercetin on human chronic gastritis and whether quercetin can relieve symptoms remain unclear.AIM To assess whether tumor necrosis factor-α(TNF-α)-induced MMP-9 expression mediates the anti-inflammatory effects of quercetin in normal human gastric mucosal epithelial cells.METHODS The normal human gastric mucosa epithelial cell line GES-1 was used to establish a normal human gastric epithelial cell model of TNF-α-induced MMP-9 protein overexpression to evaluate the antiinflammatory effects of quercetin.The cell counting Kit-8 assay was used to evaluate the effects of varying quercetin doses on cell viability in the normal GES-1 cell line.Cell migration was measured using Transwell assay.The expression of proto-oncogene tyrosine-protein kinase Src(cSrc),phospho(p)-c-Src,extracellular-signal-regulated kinase 2(ERK2),p-ERK1/2,c-Fos,p-c-Fos,nuclear factor kappa B(NF-κB/p65),and p-p65 and the effects of their inhibitors were examined using Western blot analysis and measurement of luciferase activity.p65 expression was detected by immunofluorescence.MMP-9 m RNA and protein levels were measured by quantitative reverse transcription polymerase chain reaction(q RT–PCR)and gelatin zymography,respectively.RESULTS q RT-PCR and gelatin zymography showed that TNF-αinduced MMP-9 m RNA and protein expression in a dose-and time-dependent manner.These effects were reduced by the pretreatment of GES-1 cells with quercetin or a TNF-αantagonist(TNFR inhibitor)in a dose-and timedependent manner.Quercetin and TNF-αantagonists decreased the TNF-α-induced phosphorylation of c-Src,ERK1/2,c-Fos,and p65 in a dose-and time-dependent manner.Quercetin,TNF-αantagonist,PP1,U0126,and tanshinone IIA(TSIIA)reduced TNF-α-induced c-Fos phosphorylation and AP-1–Luciferase(Luc)activity in a dose-and time-dependent manner.Pretreatment with quercetin,TNF-αantagonist,PP1,U0126,or Bay 11-7082 reduced TNF-α-induced p65 phosphorylation and translocation and p65–Luc activity in a dose-and timedependent manner.TNF-αsignificantly increased GES-1 cell migration,and these results were reduced by pretreatment with quercetin or a TNF-αantagonist.CONCLUSION Quercetin significantly downregulates TNF-α-induced MMP-9 expression in GES-1 cells via the TNFR-c-Src–ERK1/2 and c-Fos or NF-κB pathways.

Molecular mechanism of therapeutic approaches for human gastric cancer stem cells

Gastric cancer(GC)is a primary cause of cancer-related mortality worldwide,and even after therapeutic gastrectomy,survival rates remain poor.The presence of gastric cancer stem cells(GCSCs)is thought to be the major reason for resistance to anticancer treatment(chemotherapy or radiotherapy),and for the development of tumor recurrence,epithelial–mesenchymal transition,and metastases.Additionally,GCSCs have the capacity for self-renewal,differentiation,and tumor initiation.They also synthesize antiapoptotic factors,demonstrate higher performance of drug efflux pumps,and display cell plasticity abilities.Moreover,the tumor microenvironment(TME;tumor niche)that surrounds GCSCs contains secreted growth factors and supports angiogenesis and is thus responsible for the maintenance of the growing tumor.However,the genesis of GCSCs is unclear and exploration of the source of GCSCs is essential.In this review,we provide up-todate information about GCSC-surface/intracellular markers and GCSC-mediated pathways and their role in tumor development.This information will support improved diagnosis,novel therapeutic approaches,and better prognosis using GCSC-targeting agents as a potentially effective treatment choice following surgical resection or in combination with chemotherapy and radiotherapy.To date,most anti-GCSC blockers when used alone have been reported as unsatisfactory anticancer agents.However,when used in combination with adjuvant therapy,treatment can improve.By providing insights into the molecular mechanisms of GCSCs associated with tumors in GC,the aim is to optimize anti-GCSCs molecular approaches for GC therapy in combination with chemotherapy,radiotherapy,or other adjuvant treatment.

Immunomodulation of Human Carcinogenesis by the Blood Serum Antibodies against Benzo[a]pyrene, Estradiol and Progesterone

It was supposed that lung and breast cancer risks significantly increased when the levels of serum immunoglobulins A antibodies against benzo[a]pyrene and estradiol increased together, but did not separately. However, the cancer risks dramatically decreased when the levels of immunoglobulins A against progesterone elevated separately or together with immunoglobulins A against benzo[a]pyrene and estradiol. So, immunoglobulins A against benzo[a]pyrene and immunoglobulins A against estradiol acted as co-initiator and co-promoter in developing cancer scenario, but immunoglobulins A against progesterone acted along or conjointly with immunoglobulins A against benzo[a]pyrene and estradiol as strongly inhibitor in human carcinogenesis. Also it was suggested the precise mechanism of carcinogenesis modulation using anti-idiotypic antibodies against estradiol and progesterone through their membrane steroid receptors.

PAN-PVDF复合增强静电纺锂离子电池隔膜

静电纺丝纳米纤维膜作为锂离子电池隔膜使用时具有优良的性能,但是由于纤维间抱合力较小,强度较低,其应用受到了极大限制。通过耐高温相PAN与低熔点相PVDF两种聚合物混纺,并经过适当的热压后处理,使PVDF部分熔融形成点粘结,制备PAN-PVDF复合纳米纤维膜,并测试其相关性能。发现在保留静电纺膜优良性能的前提下,其强度相对处理前提高了近10倍,并表现出良好的电化学性能:室温下离子电导率达到1.32×10-3S/cm,聚合物电解质分解电压高达5.24 V,界面阻抗仅为45Ω,0.2 C首次放电比容量高达152 m Ah/g,综合性能远优于美国CELGARD2400。

实战情境课程模式对大学生体育学习情境兴趣影响的实验研究

目的:从学习兴趣培养角度验证实战情境课程模式的作用,为深化体育教学改革提供理论依据,同时,为培养学生学习兴趣提供实践指导。方法:选取某普通高校二年级114名学生为实验对象,其中实验组男女生共53人,对照组男女生共61人。通过12周教学实验干预,并运用多元协方差分析两种教学模式对大学生体育学习情境兴趣的影响。结果:在排除学生性别、体育锻炼行为阶段、排球学习经历,以及体质健康状况等因素后,组别对学习积极兴趣、消极兴趣与自主学习的主效应,具有统计学意义(F=15.410、8.764,P=0.001、0.004<0.01;F=6.197,P=0.014<0.05),而对关注与锻炼程度的主效应,具有边缘性统计学意义(F=3.415,P=0.067>0.05)。结论:实战情境课程模式有利于促进学生学习积极兴趣、自主学习与缓解学生学习消极兴趣;对关注与锻炼程度的作用不是非常显著,但存利好趋势。

美国实战情境课程模式的借鉴与启示

运用文献资料、逻辑分析等研究方法对美国实战情境课程模式的概念与理论基础、组成结构与特征、教学过程与方法等方面进行阐述。研究认为:(1)实战情境课程模式以真实比赛的技战术情境组合练习为教学内容,以问题为导向的教学方法,并通过模拟真实比赛场景练习技战术为基础、技战术综合运用为重点、教学比赛为主线来实现教学目标的一种课程模式;(2)实战情境组合练习、感知和相关关系分析以及制定决策(decisionmaking)、执行和实施决策、比赛表现四个组成部分。具有相关性、真实性、情景性、适应性和激发性等特征;(3)从单个技战术、每节课、每个教学单元等三个方面论述了具体的教学实施过程以及采用的方法;(4)美国实战情境课程模式对我国当前学校体育课程改革的启示是:用运动项目文化来统领教学、体育教学目标要具有可操作性、用比赛思维来设计体育教学内容、注重问题导向性教学方法的使用,这些对于健康中国背景下深化学校体育课程改革具有一定的参考价值。

一种筛选血管紧张素转换酶抑制肽的方法

实验建立一种从蛋白水解物中快速高效鉴别血管紧张素转化酶抑制剂(ACEI)的方法:先对蛋白按照酶解位点进行序列搜寻,建立一个样品肽库;在量效结构关系(QSAR)研究的基础上对样品库中的肽的ACE抑制活性进行预测,然后化学合成目标肽及测定活性;必要时用现代分析技术确证蛋白水解物中目标肽的存在。用此方法对油菜、大米、小麦蛋白水解物中三肽ACE抑制活性进行研究,发现IC50在10μmol/L以下的三肽多达34条,选择了11条活性较高的肽合成验证,最后发现潜藏在油菜Cruciferin BNC1(P33523)蛋白序列347～349的LRL活性最高,IC50达到3.42μmol/L,且具有较好的稳定性。抑制动力学研究表明,LRL属于竞争性抑制剂;油菜蛋白胃蛋白酶水解物进行分子质量分段,取分子质量小于1000D部分进行高效液相色谱(HPLC)分析发现有LRL。

战略性新兴产业资本配置效率研究

本文采用Jeffrey Wurgler的弹性系数法度量资本配置效率,对在上海证券交易所上市的以战略性新兴产业为主导的上海籍公司2015—2018年数据进行实证分析。结果表明,上海战略性新兴公司的产业资本配置效率呈现如下特点:在不同时期的波动较大,但基本处于正值;虽大体呈增长态势,但整体上仍低于工业平均水平,这可能是由于战略性新兴产业的发展环境和发展时期特点导致的。

静电纺丝中电荷不平衡状态及临界电压的变化

通过测试聚乙烯醇(PVA)溶液静电纺丝过程中的剩余溶液离子电导率和表面张力的变化,研究了静电纺丝过程中溶液带电性质和表面张力的变化规律,揭示了引起临界电压变化的原因。结果表明:Taylor临界压力降与纺针直径、长度、溶液的表面张力及接收距离相关外,还受临界电压及溶液的介电常数、离子电导率等带电性质的影响;静电纺丝射流形式与电源极性及电场方向无关;静电纺丝过程中的剩余溶液的带电性质是不断变化的,随着静电纺丝的进行,剩余溶液将出现电荷不平衡状态,而去除强电场后,剩余溶液将恢复电荷平衡状态。

神经管畸形儿及其父母还原叶酸载体基因多态性的FBAT关联研究

目的：探讨神经管畸形（neuraltubedefects,NTDs）儿及其父母的还原叶酸载体基因（reduced folate carrier gene,RFC1）A80G多态性在NTDs发生危险中的作用,为探讨NTDs遗传易感标记物提供流行病学依据。方法：应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术,检测104例NTDs儿及其父母和100例健康对照儿及其父母的RFC1 A80G多态性,利用病例-父母对照研究中传递/不平衡检验（TDT检验）和以家系为基础的关联检验（FBAT检验）分析NTDs儿的父母RFC1基因A80G多态性与NTDs发生危险的关联,及其在子代中传递的作用强度。结果：患儿父母均为杂合子GA/GA的比例高达36.36%,NTDs患儿成为纯合子GG的概率为25%时具有统计学意义（P〈0.05）,表明患儿接受父母遗传的G等位基因的概率均为25%,并有发生NTDs危险的可能。NTDs的TDT检验结果显示,患儿父母传递等位基因G的危险性是传递等位基因A危险性的1.56倍（95%CI：1.07~2.28）,认为该基因在亲代和NTDs子代间存在传递失衡现象。FBAT检验中,不论显性模型还是隐性模型,均未发现等位基因G与NTDs的发生危险存在关联,该结果与上述病例-父母对照研究TDT检验结果不一致。结论：虽然TDT检验中G等位基因与NTDs发生危险有关,但FBAT检验未发现G等位基因与NTDs发生危险存在关联,应进一步加大核心家系数量验证该结果。

Probiotic modulation of dendritic cells co-cultured with intestinal epithelial cells

AIM:To investigate cytokine production and cell surface phenotypes of dendritic cells (DC) in the presence of epithelial cells stimulated by probiotics.METHODS:Mouse DC were cultured alone or together with mouse epithelial cell monolayers in normal or inverted systems and were stimulated with heat-killed probiotic bacteria,Bifidobacterium lactis AD011 (BL),Bifidobacterium bifidum BGN4 (BB),Lactobacillus casei IBS041 (LC),and Lactobacillus acidophilus AD031 (LA),for 12 h.Cytokine levels in the culture supernatants were determined by enzyme-linked immunosorbent assay and phenotypic analysis of DC was investigated by flow cytometry.RESULTS:BB and LC in single-cultured DC increased the expression of I-Ad,CD86 and CD40 (I-Ad,18.51 vs 30.88,46.11;CD86,62.74 vs 92.7,104.12;CD40,0.67 vs 6.39,3.37,P < 0.05).All of the experimental probiot-ics increased the production of inflammatory cytokines,interleukin (IL)-6 and tumor necrosis factor (TNF)-α.However,in the normal co-culture systems,LC and LA decreased the expression of I-A d (39.46 vs 30.32,33.26,P < 0.05),and none of the experimental probiotics increased the levels of IL-6 or TNF-α.In the inverted coculture systems,LC decreased the expression of CD40 (1.36 vs-2.27,P < 0.05),and all of the experimental probiotics decreased the levels of IL-6.In addition,BL increased the production of IL-10 (103.8 vs 166.0,P < 0.05) and LC and LA increased transforming growth factor-β secretion (235.9 vs 618.9,607.6,P < 0.05).CONCLUSION:These results suggest that specific probiotic strains exert differential immune modulation mediated by the interaction of dendritic cells and epithelial cells in the homeostasis of gastrointestinal tract.

Oxidative stress and inflammatory signaling in cerulein pancreatitis

Oxidative stress is considered to be an important regulator of the pathogenesis of acute pancreatitis.Reactive oxygen species(ROS)regulate the activation of inflammatory cascades,the recruitment of inflammatory cells and tissue damage in acute pancreatitis.A hallmark of the inflammatory response in pancreatitis is the induction of cytokine expression,which is regulated by a number of signaling molecules including oxidant-sensitive transcription factors such as nuclear factor-κB(NF-κB)and activator protein-1(AP-1),signal transducer and activator of transcription 3(STAT3),and mitogen-activated protein kinases(MAPKs).Cross-talk between ROS and pro-inflammatory cytokines is mediated by NF-κB,AP-1,STAT3,and MAPKs;this crosstalk amplifies the inflammatory cascade in acute pancreatitis.Therapeutic studies have shown that antioxidants and natural compounds can have beneficial effects for patients with pancreatitis and can also influence the expression of proinflammatory cytokines in cerulein-induced pancreatitis.Since oxidative stress may activate inflammatory signaling pathways and contribute to thedevelopment of pancreatitis,antioxidant therapy may alleviate the symptoms or prevent the development of pancreatitis.Since chronic administration of high doses of antioxidants may have deleterious effects,dosage levels and duration of antioxidant treatment should be carefully determined.

Food intolerance and skin prick test in treated and untreated irritable bowel syndrome

瞄准：相关临床的特征对待并且有到皮肤的结果的急躁的肠症候群(IBS ) 的未经治疗的病人为食物和被吸入的东西变应原刺测试(SPT ) 。方法：我们招募了 105 个对象形成三个不同目标组：为 IBS 经历治疗的对待的组(n=44 ) ，没有治疗，为 IBS 满足罗马 II 标准的未经治疗的组(n=31 ) ，没有 IBS 症状的控制组(n=30 ) 。结果：SPT 结果在在哪个 SPT 是积极的在里面(38.6%) 17 对待病人的三个组之中是不同的，在 5 (16.1%) 未经治疗的病人并且在 1 (3.3%) 控制(P【0.01 ) 。积极 SPT 的数字比在控制组(P【0.001 ) 在 IBS 组是更大的。积极食物 SPT 的数字比在未经治疗的 IBS 组(P=0.03 ) 在对待的 IBS 组是更高的。结论：积极食物 SPT 比在控制在 IBS 病人是更高的。

Tumor progression-dependent angiogenesis in gastric cancer and its potential application

Despite improvements in the early diagnosis,prognosis and therapeutic strategies for gastric cancer(GC),human GC remains one of the most frequently diagnosed malignant tumors in the world,and the survival rate of GC patients remains very poor.Thus,a suitable therapeutic strategy for GC is important for prolonging survival.Both tumor cells themselves and the tumor microenvironment play an important role in tumorigenesis,including angiogenesis,inflammation,immunosuppression and metastasis.Importantly,these cells contribute to gastric carcinogenesis by altering the angiogenic phenotype switch.The development,relapse and spreading of tumors depend on new vessels that provide the nutrition,growth factors and oxygen required for continuous tumor growth.Therefore,a state of tumor dormancy could be induced by blocking tumor-associated angiogenesis.Recently,several antiangiogenic agents have been identified,and their potential for the clinical management of GC has been tested.Here,we provide an up-to-date summary of angiogenesis and the angiogenic factors associated with tumor progression in GC.We also review antiangiogenic agents with a focus on the anti-vascular endothelial growth factor receptor(VEGFR)-mediated pathway for endothelial cell growth and their angiogenesis ability in GC.However,most antiangiogenic agents have reported no benefit to overall survival(OS)compared to chemotherapy alone in local or advanced GC.In phase III clinical trials,only ramucirumab(anti-VEGFR blocker)and apatinib(VEGFR-TKI blocker)have reported an improved median overall response rate and prolonged OS and progression-free survival outcomes as a 2 nd-line agent combined with chemotherapy treatment in advanced GC.By providing insights into the molecular mechanisms of angiogenesis associated with tumor progression in GC,this review will hopefully aid the optimization of antiangiogenesis strategies for GC therapy in combination with chemotherapy and adjuvant treatment.

Gonadotrophin-releasing hormone-Ⅰ and -Ⅱ stimulate steroidogenesis in prepubertal murine Leydig cells in vitro

Aim： To study the effect and mechanism of gonadotrophin-releasing hormone （GnRH） on murine Leydig cell steroidogenesis. Methods： Purified murine Leydig cells were treated with GnRH-Ⅰ and -Ⅱ agonists, and testosterone production and steroidogenic enzyme expressions were determined. Results： GnRH-Ⅰ and -Ⅱ agonists significantly stimulated murine Leydig cell steroidogenesis 60%-80% in a dose- and time-dependent manner （P 〈 0.05）. The mRNA expressions of steroidogenic acute regulatory （STAR） protein, P450scc, 3β-hydroxysteroid dehydrogenase （HSD）, but not 17β-hydroxylase or 17β-HSD, were significantly stimulated by both GnRH agonists with a 1.5- to 3-fold increase （P 〈 0.05）. However, only 3β-HSD protein expression was induced by both GnRH agonists, with a 1.6- to 2-fold increase （P 〈 0.05）. Conclusion： GnRH directly stimulated murine Leydig cell steroidogenesis by activating 3β-HSD enzyme expression.

Consumption of red and processed meat and esophageal cancer risk:Meta-analysis

To summarize the evidence about the association between red and processed meat intake and the risk of esophageal cancer,we systematically searched the PubMed and EMBASE databases up to May 2012,with a restriction to English publications,and the references of the retrieved articles.We combined the studyspecific relative risks(RRs) and 95%CI,comparing the highest with the lowest categories of consumption by using a random-effects model.A total of 4 cohort studies and 23 case-control studies were included in the meta-analysis.The combined RRs(95%CI) of the cohort studies comparing the highest and lowest categories were 1.26(1.00-1.59) for red meat and 1.25(0.83-1.86) for processed meat.For the case-control studies,the combined RRs(95%CI) comparing the highest and lowest categories were 1.44(1.16-1.80)for red meat and 1.36(1.07-1.74) for processed meat.Findings from this meta-analysis suggest that a higher consumption of red meat was associated with a greater risk of esophageal cancer.

Food Safety Traceability Systems in the Maritime Catering Logistics

This paper aims to explore food safety traceability in the onboard food and beverage operations on cruise ships through a case study approach. Semi-structured interviews were conducted with galley and foodservice staff. Additionally, a self-administered questionnaire was designed and distributed to logistics managers and employees of three Egyptian cruise companies. Results indicated that cruise ships in this study use paper-based and manually-entered (electronically stored) data traceability systems. Managing food safety by effective product recall, complying with legislation, and improving product quality are among the most important perceived benefits identified by foodservice staff. Maintaining a good reputation, ensuring food safety, reducing foodborne illnesses, suppliers' commitment to quality specifications, and maintaining guest confidence are considered the most important benefits from implementing a food traceability system. The results also depicted that the challenges managers face implementing or improving a food traceability system included high cost, absence of unified traceability standards between cruise companies and food suppliers, shortage of skilled staff, and lack of knowledge and satisfaction with current foodservice operation systems onboard cruise ships. Other findings elucidated that the critical success factors for implementing traceability included strict food traceability legislation, ensuring the adequacy and correctness of traceability information, adopting a food traceability system by the cruise company management, the standardization of food traceability information identification, and technology support for food traceability systems implementation from government. Other factors were reported of less importance. This research concluded the urgent need for more cooperation between cruise companies, food suppliers, and relevant governmental authorities to adopt food traceability systems and the need of authorizing and setting compulsory standards and regulations.

Kale Juice Improves Coronary Artery Disease Risk Factors in Hypercholesterolemic Men

Objective To evaluate the effect of 3-month kale （Brassica oleracea acephala） juice supplementation on coronary artery disease risk factors among hypercholesterolemic men. Methods Thirty-two men with hypercholesterolemia （〉 200 mg/dL） were recruited after annual health examinations among the faculty and staff at university. The subjects consumed 150 mL of kale juice per day for a 12-week intervention period. Dietary and anthropometric assessments were performed and blood samples were collected to evaluate biochemical profiles before and after supplementation. Results Serum concentrations of HDL-cholesterol, and HDL- to LDL-cholesterol ratio were significantly increased by 27% （P〈0.0001） and 52% （P〈0.0001）, respectively. The LDL-cholesterol concentration and the atherogenic index were significantly reduced by 10% （P=0.0007） and 24.2% （P〈0.0001）, respectively without affecting body mass index, waist and hip circumferences, or nutrient intakes after three months of supplementation. While there was no difference in the concentration of malondialdehyde, significant increase in glutathione peroxidase activity （P=0.0005） were accompanied by a significant increase in the serum selenium level （P=0.0132）. It was also found that the responses of these risk factors to kale juice administration were dependent on smoking status. Conclusion Regular meals supplementation with kale juice can favorably influence serum lipid profiles and antioxidant systems, and hence contribute to reduce the risks of coronary artery disease in male subjects with hyperlipidemia.