CLINICAL AND EXPERIMENTAL OBSERVATIONS ON TREATMENT OF PEPTIC ULCER WITH WEI YANG AN (EASING PEPTIC-ULCER) CAPSULE

The therapeutic effects of peptic ulcer treated with cimetidine and Wei Yang An (胃疡安) werecompared clinically and experimentally by the double blind method.Results showed that the ef-fects of the two are similar,but the long-term effect of Wei Yang An was better.Wei Yang Anwas also good for most cimetidine resistant ulcers,with few side effect.Animal experiment show-ed that Wei Yang An inhibited the ulcers.

Gene expression profiling of HepG_(2) cells after treatment with black tea polyphenols

This study aimed to determine the effects of black tea polyphenols on gene expression in hepatocellular cancer cells.The total RNA from HepG_(2) hepatocellular cancer cells treated with black tea polyphenols was subjected to Human 14K cDNA microarray analysis.Real-time PCR and Western blot analysis were conducted to verify microarray data.Black tea polyphenols treatment at the dose of 20 mg/L,40 mg/L or 80 mg/L for one to three days inhibited the growth of HepG_(2) cells in a dose and time dependent manner.A total of 48 genes showed more than two-fold change after black tea polyphenols treatment,including 17 upregulated genes and 31 downregulated genes,and they were involved in the regulation of cell growth,cell cycle,apoptosis,signaling,angiogenesis,tumor invasion and metastasis.Real-time PCR analysis of the selected genes showed that their mRNA expression changes were consistent with the microarray data.In addition,Western blot analysis of the selected genes showed that their protein expression changes were consistent with mRNA expression.In conclusion,gene expression profiles provide comprehensive molecular mechanisms by which black tea polyphenols exerts growth inhibition effects on cancer cells.The novel molecular targets identified in this study may be further exploited as therapeutic strategies for hepatocellular cancer.