InraPorc模型和NRC模型中猪的氨基酸需要量

某种程度上,研究理想蛋白质模型的方法应该是一种动态的方式。本文比较了Inra Porc模型和NRC模型在猪不同生长阶段的氨基酸需要量;阐述了配制日粮配方是根据需要量或是生长性能以及选择何种模型需要使用者去更好地判断。

Drought events influence nutrient canopy exchanges and green leaf partitioning during senescence in a deciduous forest

The increase in the frequency and intensity of drought events expected in the coming decades in Western Europe may disturb forest biogeochemical cycles and create nutrient deficiencies in trees.One possible origin of nutrient deficiency is the disturbance of the partitioning of the green leaf pool during the leaf senescence period between resorption,foliar leaching and senesced leaves.However,the effects of drought events on this partitioning and the consequences for the maintenance of tree nutrition are poorly documented.An experiment in a beech forest in Meuse(France)was conducted to assess the effect of drought events on nutrient canopy exchanges and on the partitioning of the green leaf pool during the leaf senescence period.The aim was to identify potential nutritional consequences of droughts for trees.Monitoring nutrient dynamics,including resorption,chemistry of green and senesced leaves,foliar absorption and leaching in mature beech stands from 2012 to 2019 allowed us to compare the nutrient exchanges for three nondry and three dry years(i.e.,with an intense drought event during the growing season).During dry years,we observed a decrease by almost a third of the potassium(K)partitioning to resorption(i.e.resorption efficiency),thus reducing the K reserve in trees for the next growing season.This result suggests that with the increased drought frequency and intensity expected for the coming decades,there will be a risk of potassium deficiency in trees,as already observed in a rainfall exclusion experiment on the same study site.Reduced foliar leaching and higher parititioning to the senesced leaves for K and phosphorus(P)were also observed.In addition,a slight increase in nitrogen(N)resorption efficiency occurred during dry years which is more likely to improve tree nutrition.The calcium(Ca)negative resorption decreased,with no apparent consequence in our study site.Our results show that nutrient exchanges in the canopy and the partitioning of the green leaf pool can be modified by drought events,and may have consequences on tree nutrition.

Exploring the effects of dietary inulin in rainbow trout fed a high‑starch,100%plant‑based diet

Background High dietary carbohydrates can spare protein in rainbow trout(Oncorhynchus mykiss)but may affect growth and health.Inulin,a prebiotic,could have nutritional and metabolic effects,along with anti-inflammatory properties in teleosts,improving growth and welfare.We tested this hypothesis in rainbow trout by feeding them a 100%plant-based diet,which is a viable alternative to fishmeal and fish oil in aquaculture feeds.In a two-factor design,we examined the impact of inulin(2%)as well as the variation in the carbohydrates(CHO)/plant protein ratio on rainbow trout.We assessed the influence of these factors on zootechnical parameters,plasma metabolites,gut microbiota,production of short-chain fatty acids and lactic acid,as well as the expression of free-fatty acid receptor genes in the mid-intestine,intermediary liver metabolism,and immune markers in a 12-week feeding trial.Results The use of 2%inulin did not significantly change the fish intestinal microbiota,but interestingly,the high CHO/protein ratio group showed a change in intestinal microbiota and in particular the beta diversity,with 21 bacterial genera affected,including Ralstonia,Bacillus,and 11 lactic-acid producing bacteria.There were higher levels of butyric,and valeric acid in groups fed with high CHO/protein diet but not with inulin.The high CHO/protein group showed a decrease in the expression of pro-inflammatory cytokines(il1b,il8,and tnfa)in liver and a lower expression of the genes coding for tight-junction proteins in mid-intestine(tjp1a and tjp3).However,the 2%inulin did not modify the expression of plasma immune markers.Finally,inulin induced a negative effect on rainbow trout growth performance irrespective of the dietary carbohydrates.Conclusions With a 100%plant-based diet,inclusion of high levels of carbohydrates could be a promising way for fish nutrition in aquaculture through a protein sparing effect whereas the supplementation of 2%inulin does not appear to improve the use of CHO when combined with a 100%plant-based diet.

Remote sensing of quality traits in cereal and arable production systems:A review

Cereal is an essential source of calories and protein for the global population.Accurately predicting cereal quality before harvest is highly desirable in order to optimise management for farmers,grading harvest and categorised storage for enterprises,future trading prices,and policy planning.The use of remote sensing data with extensive spatial coverage demonstrates some potential in predicting crop quality traits.Many studies have also proposed models and methods for predicting such traits based on multiplatform remote sensing data.In this paper,the key quality traits that are of interest to producers and consumers are introduced.The literature related to grain quality prediction was analyzed in detail,and a review was conducted on remote sensing platforms,commonly used methods,potential gaps,and future trends in crop quality prediction.This review recommends new research directions that go beyond the traditional methods and discusses grain quality retrieval and the associated challenges from the perspective of remote sensing data.

晚第四纪热带西太平洋Noelaerhabdaceae科颗石长度和质量变化及其影响因素

选取热带西太平洋暖池北部边缘西菲律宾海本哈姆高原(Benham Rise)MD06-3050站位的柱状样沉积物样品,利用双向圆偏光法拍摄并合成图像,通过SYRACO人工智能软件自动识别并测量和计算Noelaerhabdaceae科颗石的平均长度和平均质量。研究结果显示,自35万年以来,Noelaerhabdaceae科平均颗石长度和质量的变化趋势具有较强的相似性,两者的冰期/间冰期变化特征均不明显。根据颗石长度和质量计算得到的颗石钙化作用指标曲线与前两者的变化趋势也存在一定的相似性,说明研究海区颗石的平均质量和长度均可作为指示颗石藻钙化作用程度的指标。通过与冰芯记录的大气pCO_(2)变化曲线进行对比,发现在大气pCO_(2)浓度较高的冰期终止期以及间冰期早期,该科颗石的钙化作用相对较低,表明pCO_(2)在一定程度上影响了颗石藻的钙化作用。此外,对颗石的平均长度和质量进行23 ka周期滤波,两滤波曲线的变化与北纬15°夏季平均日照辐射曲线的变化较为一致,指示了地球天文轨道参数对颗石藻演化的调控作用。

Punch‑excised explants of bovine mammary gland to model early immune response to infection

Background Mammary gland(MG)infections(mastitis)are frequent diseases of dairy cows that affect milk quality,animal welfare and farming profitability.These infections are commonly associated with the bacteria Escherichia coli and Staphylococcus aureus.Different in vitro models have been used to investigate the early response of the MG to bacteria,but the role of the teat in mastitis pathogenesis has received less attention.In this study,we used punch-excised teat tissue as an ex vivo model to study the immune mechanisms that arise early during infection when bacteria have entered the MG.Results Cytotoxicity and microscopic analyses showed that bovine teat sinus explants have their morphology and viability preserved after 24 h of culture and respond to ex vivo stimulation with TLR-agonists and bacteria.LPS and E.coli trigger stronger inflammatory response in teat when compared to LTA and S.aureus,leading to a higher produc-tion of IL-6 and IL-8,as well as to an up-regulation of proinflammatory genes.We also demonstrated that our ex vivo model can be applied to frozen-stored explants.Conclusions In compliance with the 3Rs principle(replacement,reduction and refinement)in animal experimenta-tion,ex vivo explant analyses proved to be a simple and affordable approach to study MG immune response to infec-tion.This model,which better reproduces organ complexity than epithelial cell cultures or tissue slices,lends itself particularly well to studying the early phases of the MG immune response to infection.

Thermal conditioning of quail embryos has transgenerational and reversible long‑term effects

Background In the current context of global warming,thermal manipulation of avian embryos has received increasing attention as a strategy to promote heat tolerance in avian species by simply increasing the egg incubation temperature.However,because of their likely epigenetic origin,thermal manipulation effects may last more than one generation with consequences for the poultry industry.In this work,a multigenerational and transgenerational analysis of thermal manipulation during embryogenesis was performed to uncover the long-term effects of such procedure.Results Thermal manipulation repeated during 4 generations had an effect on hatchability,body weight,and weight of eggs laid in Japanese quails,with some effects increasing in importance over generations.Moreover,the effects on body weight and egg weight could be transmitted transgenerationally,suggesting non-genetic inheritance mechanisms.This hypothesis is reinforced by the observed reversion of the effect on growth after five unexposed generations.Interestingly,a beneficial effect of thermal manipulation on heat tolerance was observed a few days after hatching,but this effect was not transgenerational.Conclusions Our multigenerational study showed that thermal conditioning of quail embryos has a beneficial effect on post-hatch heat tolerance hampered by transgenerational but reversible defects on growth.Assuming that no genetic variability underlies these changes,this study provides the first demonstration of epigenetic inheritance of traits induced by environmental temperature modification associated with long-term impacts in an avian species.

Little Secrets for the Successful Industrial Use of Tannin Adhesives:A Review

This brief article reviews a very particular and quite narrowfield,namely what has been done and what is needed to know for tannin adhesives for wood panels to succeed industrially.The present fashionable focus on bioadhe-sives has led to producing chemical adhesive formulations and approaches for tannin adhesives as a subject of academic publications.These,as good and original they might be,are and will still remain a rather empty aca-demic exercise if not put to the test of real industrial trials and industrial use.They will remain so without the“little”secrets and techniques outlined here that show that there is a great gap between developing an adhesive formulation in the laboratory and the hard reality to make it work where it does really count,in its industrial application.It outlines the fact that even more modern and excellent,newly developed bioadhesive formulations might well miserably fail once tried in the industry if the problems that always arise in their upgrading are not identified and solved,and solved well.It also outlines the fact that not only must costs always be taken into account and that a practical and possibly easy-to-handle approach must always be used,but too expensive or complex and unyielding adhesive systems are also often shown to be unusable or unsuitable in industry.

Genome-wide association mapping in a sweet cherry germplasm collection(Prunus avium L.)reveals candidate genes for fruit quality traits

In sweet cherry(Prunus avium L.),large variability exists for various traits related to fruit quality.There is a need to discover the genetic architecture of these traits in order to enhance the efficiency of breeding strategies for consumer and producer demands.With this objective,a germplasm collection consisting of 116 sweet cherry accessions was evaluated for 23 agronomic fruit quality traits over 2-6 years,and characterized using a genotyping-by-sequencing approach.The SNP coverage collected was used to conduct a genome-wide association study using two multilocus models and three reference genomes.We identified numerous SNP-trait associations for global fruit size(weight,width,and thickness),fruit cracking,fruit firmness,and stone size,and we pinpointed several candidate genes involved in phytohormone,calcium,and cell wall metabolisms.Finally,we conducted a precise literature review focusing on the genetic architecture of fruit quality traits in sweet cherry to compare our results with potential colocalizations of marker-trait associations.This study brings new knowledge of the genetic control of important agronomic traits related to fruit quality,and to the development of marker-assisted selection strategies targeted towards the facilitation of breeding efforts.

Full-fat insect meals in ruminant nutrition:in vitro rumen fermentation characteristics and lipid biohydrogenation

Background:The most used protein sources in ruminant nutrition are considered as having negative impacts in terms of environmental sustainability and competition with human nutrition.Therefore,the investigation of alternative and sustainable feedstuffs is becoming a priority in ruminant production systems.Results:This trial was designed to evaluate eight full-fat insect meals(Acheta domesticus–ACD;Alphitobius diaperinus–ALD;Blatta lateralis–BL;Gryllus bimaculatus–GB;Grylloides sygillatus–GS;Hermetia illucens–HI;Musca domestica–MD;and Tenebrio molitor–TM)as potential protein and lipid sources in ruminant nutrition.Fermentation parameters and fatty acids(FA)of rumen digesta after 24-h in vitro ruminal incubation of the tested insect meals were measured and compared with those of three plant-based meals(soybean meal,rapeseed meal and sunflower meal)and fishmeal(FM).Similarly to FM,the insect meals led to a significantly lower total gas production(on average,1.75vs.4.64 mmol/g dry matter-DM),methane production(on average,0.33 vs.0.91 mmol/g DM),volatile FA production(on average,4.12 vs.7.53 mmol/g DM),and in vitro organic matter disappearance(on average,0.32 vs.0.59 g/g)than those observed for the plant meals.The insect meals also led to lower ammonia of rumen fluid,when expressed as a proportion of total N(on average,0.74 vs.0.52 for the plant and insect meals,respectively),which could be an advantage provided that intestinal digestibility is high.Differences in ruminal fermentation parameters between the insect meals could be partially explained by their chitin,crude protein and ether extract contents,as well as by their FA profile.In particular,high content of polyunsaturated FA,or C12:0(in HI),seems to partially inhibit the ruminal fermentations.Conclusions:The tested full-fat insect meals appear to be potentially an interesting protein and lipid source for ruminants,alternative to the less sustainable and commonly used ones of plant origin.The FA profile of the rumen digesta of ACD,ALD,GB,GS and TM,being rich in n-6 polyunsaturated FA,could be interesting to improve the quality of ruminant-derived food products.

Morphodynamics and sediment connectivity index in an unmanaged,debris-flow prone catchment:a through time perspective

Torrential processes are among the main actors responsible for sediment production and mobility in mountain catchments.For this reason,the understanding of preferential pathways for sediment routing has become a priority in hazard assessment and mitigation.In this context,the sediment Connectivity Index(IC)enables to analyse the existing linkage between sediment sources and the selected target(channel network or catchment outlet).The IC is a grid-based index that allows fast computation of sediment connectivity based on landscape information derived from a single Digital Terrain Model(DTM).The index computation is based on the log-ratio between an upslope and a downslope component,including information about drainage area,slope,terrain roughness,and distance to the analysis target(e.g.outlet).The output is a map that highlights the degree of structural connectivity of sediment pathways over analysed catchments.Until now,these maps are however rarely used to help defining debris-flow hazard maps,notably due to a lack of guidelines to interpret the IC spatial distribution.This paper proposes an exploitation procedure along profiles to extract more information from the analysis of mapped IC values.The methodology relies on the analysis of the IC and its component variables along the main channel profile,integrated with information about sediment budgeting derived from Difference of DEMs(DoD).The study of connectivity was applied in the unmanaged sub-catchment(without torrent control works)of the Rio Soial(Autonomous Province of Trento–NE Italy)to understanding the geomorphic evolution of the area after five debris flows(in ten years)and the related changes of sediment connectivity.Using a recent DTM as validation,we demonstrated how an IC analysis over the older DTM can help predicting geomorphic changes and associated hazards.The results show an IC aptitude to capture geomorphic trajectories,anticipate debris flow deposits in a specific channel location,and depict preferential routing pathways.

Modification of Wood by Tannin-Furfuryl Alcohol Resins-Effect on Dimensional Stability,Mechanical Properties and Decay Durability

Furfurylation is a well-known wood modification technology.This paper studied the effect of tannin addition on the wood furfurylation.Three kinds of dicarboxylic acids,adipic acid,succinic acid,and tartaric acid,as well as glyoxal as a comparing agent,were used to catalyse the polymerisation of furanic or tannin-furanic solutions during wood modification.Impregnation of furanic or tannin-furanic solution at a certain concentration into the wood followed with curing at 103℃for a specific duration was performed for the wood modification.Different properties of the modified woods like dimensional stability,resistance of treatment to leaching,mechanical properties,decay durability against white-rot(Coriolus versicolor,Pycnoporus sanguineus)and brown-rot(Coniophora puteana)as well as their chemical and anatomical characteristics were evaluated.Results revealed that the partial substitution of FA by the tannins improved the fixation of the chemicals impregnated in wood.Further,dimensional stability,leaching resistance,Brinell hardness,modulus of elasticity/modulus of rupture,and decay durability properties of the furfurylated wood were also improved in the presence of tannins.Scanning electron microscopy revealed the deposition of the polymer in the wood lumen cells and in the wood cell walls.

Towards a Modeling of the Impacts of Road Verge Management on the Pollination Service Using System Dynamics: A Case Study in France

Several research studies have proven that eliciting and predicting the impact of human activity on ecosystem services will be crucial to support stakeholders’ awareness and to decide how to interact with the environment in a more sustainable manner. In this sense, the ecosystems known as road verges are particularly important because of their length and surface at an international scale, and their role in mitigating the damage done by roads. Plant pollination by insects is one of the most important ecosystem services. Because of its nature and the fact that they extend across a variety of landscapes, roadside can contribute to the maintenance of healthy ecosystems, under the condition of adapted management practices. This research is the first attempt to develop a System Dynamics-based aiming to estimate the ecological and economic impact of maintenance on the road verge pollination service in France. Maintenance strategies of road verges are simulated to compare their performance. The results show that there are ways to improve current maintenance strategies in terms of pollination value, but also that the model needs to consider other ecosystem services and synergistic effects that could further affect pollination to obtain more accurate estimations.

Shared-role of vegetation types,elevation and soil affecting plant diversity in an old-tropical mountain hotspot

Despite the exceptional species richness and endemism,the environmental drivers of plant diversity along old tropical mountains remain underexplored.The respective importance of vegetation types,elevation,slope,and soil to drive diversity across life-forms is poorly addressed.Here,we tested whether environmental variables drove local and regional plant diversity along an old tropical mountain according to the three main life-forms:graminoids,herbaceous and woody species.We sampled all Angiosperm species on 180 plots across five elevations,at the tropical old-mountain region of Serra do Cipó,South-eastern Brazil.We assessed soil,slope,and vegetation types,and calculated richness and beta-diversity,applying generalized least square models,linear mixed-models and partial Mantel tests to test for relationships.Richness of graminoids and herbaceous species increased with greater elevation and more nutrient-impoverished soils,while woody richness showed the inverse pattern.Beta-diversity was primarily driven by species turnover,correlated with elevation and soil and higher in less dominant vegetation types,with unique species.Despite the limited elevational range in these old mountains,it still played an important role in filtering woody species,while fostering graminoid and herbaceous species.Conservation and restoration actions need to foster the high regional diversity supported by the old mountain heterogeneous landscape and the diversity of life-forms,especially the dominant and highly diverse grassy component.

First Report of a Successful Development of Yam Hybrids(Dioscorea alata L.)from Lyophilized and Long-Term Stored Pollens

Various biological constraints including erratic and asynchronous flowering between male and female plants hinder successful hybrid development and genetic gains in greater yam breeding programs.Therefore,pollen storage has gained much attention to facilitate artificial pollinations and increase the genetic gains.This 4-year study aimed at developing a practical long-term pollen storage technique for the successful development of yam hybrids.Fresh pollens were collected from two Dioscorea alata males,then lyophilized(two lyophilization treatments were applied),followed by storage at room temperature(24℃–25℃)for 12 months.Moreover,the lyophilized and stored pollens were tested for viability by crossing with four female varieties.Our results showed that lyophilization is effective for achieving viable pollens after 12 months of storage.Treatment 1(48 h drying)showed higher pollen germination and fertility rates than Treatment 2(72 h drying).Although we observed a reduction in viability of lyophilized pollens after 12 months of storage,we generated hybrid seedlings with success rates from 12%to 21%compared to 21%–31%when using fresh pollens.Paternity testing based on molecular genotyping confirmed the hybrid status of the obtained seedlings,which grew well in a greenhouse.Lyophilization is a practical approach for a long-term storage of greater yam pollen samples.This protocol will positively impact yam breeding programs particularly in developing countries.

BABA-induced pathogen resistance: a multi-omics analysis of the tomato response reveals a hyper-receptive status involving ethylene

Prior exposure to microbial-associated molecular patterns or specific chemical compounds can promote plants into a primed state with stronger defence responses.β-aminobutyric acid(BABA)is an endogenous stress metabolite that induces resistance protecting various plants towards diverse stresses.In this study,by integrating BABA-induced changes in selected metabolites with transcriptome and proteome data,we generated a global map of the molecular processes operating in BABA-induced resistance(BABA-IR)in tomato.BABA significantly restricts the growth of the pathogens Oidium neolycopersici and Phytophthora parasitica but not Botrytis cinerea.A cluster analysis of the upregulated processes showed that BABA acts mainly as a stress factor in tomato.The main factor distinguishing BABA-IR from other stress conditions was the extensive induction of signaling and perception machinery playing a key role in effective resistance against pathogens.Interestingly,the signalling processes and immune response activated during BABA-IR in tomato differed from those in Arabidopsis with substantial enrichment of genes associated with jasmonic acid(JA)and ethylene(ET)signalling and no change in Asp levels.Our results revealed key differences between the effect of BABA on tomato and other model plants studied until now.Surprisingly,salicylic acid(SA)is not involved in BABA downstream signalization whereas ET and JA play a crucial role.

Intrauterine growth retardation affects liver bile acid metabolism in growing pigs:effects associated with the changes of colonic bile acid derivatives

Background:Intrauterine growth retardation(IUGR)is associated with severely impaired nutrient metabolism and intestinal development of pigs.Our previous study found that IUGR altered intestinal microbiota and metabolites in the colon.However,the consequences of IUGR on bile acid metabolism in pigs remained unclear.The present study aimed to investigate the bile acid metabolism in the liver and the profile of bile acid derivatives in the colon of grow-ing pigs with IUGR using bile acid targeted metabolomics.Furthermore,we determined correlations between colonic microbiota composition and metabolites of IUGR and normal birth weight(NBW)pigs at different growth stages that were 7,21,and 28-day-old,and the average body weight(BW)of 25,50,and 100 kg of the NBW pigs.Results:The results showed that the plasma total bile acid concentration was higher(P<0.05)at the 25 kg BW stage and tended to increase(P=0.08)at 28-day-old in IUGR pigs.The hepatic gene expressions related to bile acid synthe-sis(CYP7A1,CYP27A1,and NTCP)were up-regulated(P<0.05),and the genes related to glucose and lipid metabolism(ATGL,HSL,and PC)were down-regulated(P<0.05)at the 25 kg BW stage in IUGR pigs when compared with the NBW group.Targeted metabolomics analysis showed that 29 bile acids and related compounds were detected in the colon of pigs.The colonic concentrations of dehydrolithocholic acid and apocholic acid were increased(P<0.05),while isodeoxycholic acid and 6,7-diketolithocholic acid were decreased(P<0.05)in IUGR pigs,when compared with the NBW pigs at the 25 kg BW stage.Moreover,Spearman’s correlation analysis revealed that colonic Unclassified_[Mogi-bacteriaceae],Lachnospira,and Slackia abundances were negatively correlated(P<0.05)with dehydrolithocholic acid,as well as the Unclassified_Clostridiaceae abundance with 6,7-diketolithocholic acid at the 25 kg BW stage.Conclusions:These findings suggest that IUGR could affect bile acid and glucolipid metabolism in growing pigs,especially at the 25 kg BW stage,these effects being paralleled by a modification of bile acid derivatives concentra-tions in the colonic content.The plausible links between these modified parameters are discussed.

Integrated model simulates bigger, sweeter tomatoes under changing climate under reduced nitrogen and water input

When simulating the response of fruit growth and quality to environmental factors and cultivation practices,the interactions between the mother plant and fruit need to be considered as a whole system.Here,we developed the integrative Tomato plant and fruit Growth and Fruit Sugar metabolism(TGFS)model by coupling equations describing the biophysical processes of leaf gas exchange,water transport,carbon allocation,organ growth and fruit sugar metabolism.The model also accounts for effects of soil nitrogen and atmospheric CO_(2) concentration on gaseous exchange of water and carbon by the leaf.With different nitrogen and water input values,TGFS performed well at simulating the dry mass of the tomato leaf,stem,root,and fruit,and the concentrations of soluble sugar and starch in fruit.TGFS simulations showed that increasing air temperature and CO_(2) concentration has positive effects on fruit growth,but not on sugar concentrations.Further model-based analyses of cultivation scenarios suggest that,in the context of climate change,decreasing N by 15%–25%and decreasing irrigation by 10%–20%relative to current levels would increase tomato fresh weight by 27.8%–36.4% while increasing soluble sugar concentration by up to 10%.TGFS provides a promising tool to optimise N and water inputs for sustainable high-quality tomatoes.

Performance of a Solar-Biomass Adsorption Chiller

A dynamic model is presented for a chiller working with a composite adsorbent(silica activated carbon/CaCl2)–water pair in a solar-biomass cooling installation.The main objective is determining a link between two possible evaporator configurations and the refrigerator’s performances.The two considered evaporators work at different pressure levels.The related time evolution profiles of temperature,pressure and water content are studied.Moreover,the effects of hot water inlet temperature and cooling water inlet temperature on the specific cooling capacity(SCP)and coefficient of performance(COP)are predicted by means of numerical simulations.The results show that an increase in the temperature of hot water and a decrease in the temperature of the cooling water allow an increase in COP and SCP.In particular,for a hot water inlet temperature of 85°C and a cooling water inlet temperature of 40°C,the COP and Qev are 0.67 and 4.3 kW,respectively.