Irradiated seeds of Phaseolus vulgaris cv. Rajmah using Synchroton X-Ray Beam (BL-07) at RRCAT, Indore at various doses in the range of 0.5 - 10 Gy were used to raise the seedling and the growth status at different st...Irradiated seeds of Phaseolus vulgaris cv. Rajmah using Synchroton X-Ray Beam (BL-07) at RRCAT, Indore at various doses in the range of 0.5 - 10 Gy were used to raise the seedling and the growth status at different stages was evaluated. Prior to germination, in the seeds soaked for 24 hours, the water regain remained unaffected by seed irradiation at 1 - 10 Gy doses, while the acid phosphatase activity was significantly reduced. Strong correlation (R2 = 0.685) between irradiation dose and enzyme activity also resulted. Analysing seed irradiation effect on seed development up to 4 days, % germination, germination index (GI), seedling wt, and seedling vigour were non significantly decreased at 5.0 Gy dose. The overall growth of 10 days old seedlings raised from irradiated seeds was substantially reduced at irradiation doses of 2?and 5 Gy exerting strong -ve correlation. Also % germination and seed vigour index (SVI) were prominently decreased due to seed irradiation. The nitrogen status of the seedlings, reflected by nitrate reductase activity (NRA) was significantly reduced in response to irradiation exerting strong correlation. The results demonstrate decreased phosphate mobilization in soaked seeds, time dependent decreased growth being more substantial with longer duration and reduced nitrate assimilation due to seed irradiation with Synchroton X-Ray Beam.展开更多
Irradiated seeds of Phaseolus vulgaris cv. Rajmah using Synchrotron X Ray Beam (BL-07) in RRCAT, Indore at doses of 1, 10 and 20 Gy were used to raise the seedling and the effects on growth and biochemical constituent...Irradiated seeds of Phaseolus vulgaris cv. Rajmah using Synchrotron X Ray Beam (BL-07) in RRCAT, Indore at doses of 1, 10 and 20 Gy were used to raise the seedling and the effects on growth and biochemical constituents in 4 - 8 days, old seedlings were analyzed. The seed irradiation effect on seedling development up to about 4 - 5 days, % germination, seedling length and seedling vigor are significantly decreased at 10 and 20 Gy doses with strong -ve correlation. Other parameters, like relative water content, electrical conductivity and acid phosphatase activity are also decreased. Decrease in various biochemical constituents, like, protein and proline has shown significant reduction at 10 and 20 Gy and phenol at 1 - 20 Gy. However, peroxidase activity is increased at 1 and 10 Gy. Amongst the antioxidant enzymes, only superoxide dismutase activity has shown significant increase at 10 and 20 Gy. For seed irradiation (1 and 10 Gy) effect on seedling development up to 8 days involving transfer to hydroponic culture after 4 days, in shoot tissue, decrease in nitrate reductase activity and pigment content is observed, while nitrate reductase activity in root tissue is increased. The results demonstrate adverse effects on growth as well as biochemical constituents along with increased antioxidant effect in bean seedlings with irradiation of seeds at high dose of synchrotron X radiations. Also the nitrate assimilation and photosynthetic activity are reduced in shoot tissue with seed irradiation, however, increased nitrate reductase activity in roots suggests the involvement of NO signaling.展开更多
文摘Irradiated seeds of Phaseolus vulgaris cv. Rajmah using Synchroton X-Ray Beam (BL-07) at RRCAT, Indore at various doses in the range of 0.5 - 10 Gy were used to raise the seedling and the growth status at different stages was evaluated. Prior to germination, in the seeds soaked for 24 hours, the water regain remained unaffected by seed irradiation at 1 - 10 Gy doses, while the acid phosphatase activity was significantly reduced. Strong correlation (R2 = 0.685) between irradiation dose and enzyme activity also resulted. Analysing seed irradiation effect on seed development up to 4 days, % germination, germination index (GI), seedling wt, and seedling vigour were non significantly decreased at 5.0 Gy dose. The overall growth of 10 days old seedlings raised from irradiated seeds was substantially reduced at irradiation doses of 2?and 5 Gy exerting strong -ve correlation. Also % germination and seed vigour index (SVI) were prominently decreased due to seed irradiation. The nitrogen status of the seedlings, reflected by nitrate reductase activity (NRA) was significantly reduced in response to irradiation exerting strong correlation. The results demonstrate decreased phosphate mobilization in soaked seeds, time dependent decreased growth being more substantial with longer duration and reduced nitrate assimilation due to seed irradiation with Synchroton X-Ray Beam.
文摘Irradiated seeds of Phaseolus vulgaris cv. Rajmah using Synchrotron X Ray Beam (BL-07) in RRCAT, Indore at doses of 1, 10 and 20 Gy were used to raise the seedling and the effects on growth and biochemical constituents in 4 - 8 days, old seedlings were analyzed. The seed irradiation effect on seedling development up to about 4 - 5 days, % germination, seedling length and seedling vigor are significantly decreased at 10 and 20 Gy doses with strong -ve correlation. Other parameters, like relative water content, electrical conductivity and acid phosphatase activity are also decreased. Decrease in various biochemical constituents, like, protein and proline has shown significant reduction at 10 and 20 Gy and phenol at 1 - 20 Gy. However, peroxidase activity is increased at 1 and 10 Gy. Amongst the antioxidant enzymes, only superoxide dismutase activity has shown significant increase at 10 and 20 Gy. For seed irradiation (1 and 10 Gy) effect on seedling development up to 8 days involving transfer to hydroponic culture after 4 days, in shoot tissue, decrease in nitrate reductase activity and pigment content is observed, while nitrate reductase activity in root tissue is increased. The results demonstrate adverse effects on growth as well as biochemical constituents along with increased antioxidant effect in bean seedlings with irradiation of seeds at high dose of synchrotron X radiations. Also the nitrate assimilation and photosynthetic activity are reduced in shoot tissue with seed irradiation, however, increased nitrate reductase activity in roots suggests the involvement of NO signaling.
