After maturation, Korean pine (Pinus koraiensis Siebold & Zucc.) seeds often cannot disperse to reach a suitable germination site. It is therefore difficult to naturally regenerate by itself and relies on animal d...After maturation, Korean pine (Pinus koraiensis Siebold & Zucc.) seeds often cannot disperse to reach a suitable germination site. It is therefore difficult to naturally regenerate by itself and relies on animal dispersal. Squirrels hoard pine seeds as winter food and Korean pine seeds stored for overwintering might become the source of regeneration of the species. From field observations, the pine seeds are the food preference for squirrels during autumn in the Lesser Khingan Mountains in Northeast China. Such preference by squirrels has been attributed to high seed energy content and the ease of seed storage. However, it may also be expected from nutritional aspects that a coevolutionary relationship between squirrels and Korean pine species could be partially explained by the effect of active seed components and their physiological regulatory effects on squirrels. To test this hypothesis, control experiments were carried out to examine the modulatory effects of Korean pine nut oil (PNO) on intestinal microbiota, inflammatory profile and oxidative stress in mice. The results showed that, compared with mice fed a high-fat diet, PNO significantly improved the physical and the healthy state of mice. Histological analysis of the liver and epididymal fat tissue showed that PNO alleviated liver and adipocyte lesions, and inflammation caused by high-fat diets. PNO also significantly decreased atherosclerotic index and ameliorate serum lipid accumulation to prevent cardiovascular disease, which effect the positive control SG group. Moreover, PNO elevated superoxide dismutase and glutathione peroxidase enzyme activities and reduced malondialdehyde levels in the serum. 16S rRNA sequencing showed that PNO restored intestinal microbiota composition, significantly increasing the relative abundance of Lactobacillus and Akkermansia bacteria. These results suggest that Korean pine seeds not only provide adequate fat, protein and energy for squirrels, but also promote physical health and improve body immunity.展开更多
Correction to:J.For.Res.https://doi.org/10.1007/s11676-021-01380-2 In the Original publication,the author has found that Table 8 has been inadvertently published with errors due to incorrect calculation.The corrected ...Correction to:J.For.Res.https://doi.org/10.1007/s11676-021-01380-2 In the Original publication,the author has found that Table 8 has been inadvertently published with errors due to incorrect calculation.The corrected Table 8 is provided below:Eff ect of PNO supplements on serum lipid levels and the atherosclerosis index TC,TG and LDL-C levels increased,while the HDL-C levels decreased notably in the HFD group compared to the ND group(P<0.01;Table 8).After supplementation with PNO,serum lipid(TG,TC)levels of mice in the M-PNO and H-PNO groups decreased;the diff erences were statistically signifi cant compared with those in the HFD group(P<0.05).Furthermore,PNO supplements signifi-cantly improved HDL-C levels and decreased LDL-C levels(P<0.01).展开更多
基金This work was supported by the Heilongjiang Province Hundred Million Engineering Science and Technology Major Project(2020ZX07B01).
文摘After maturation, Korean pine (Pinus koraiensis Siebold & Zucc.) seeds often cannot disperse to reach a suitable germination site. It is therefore difficult to naturally regenerate by itself and relies on animal dispersal. Squirrels hoard pine seeds as winter food and Korean pine seeds stored for overwintering might become the source of regeneration of the species. From field observations, the pine seeds are the food preference for squirrels during autumn in the Lesser Khingan Mountains in Northeast China. Such preference by squirrels has been attributed to high seed energy content and the ease of seed storage. However, it may also be expected from nutritional aspects that a coevolutionary relationship between squirrels and Korean pine species could be partially explained by the effect of active seed components and their physiological regulatory effects on squirrels. To test this hypothesis, control experiments were carried out to examine the modulatory effects of Korean pine nut oil (PNO) on intestinal microbiota, inflammatory profile and oxidative stress in mice. The results showed that, compared with mice fed a high-fat diet, PNO significantly improved the physical and the healthy state of mice. Histological analysis of the liver and epididymal fat tissue showed that PNO alleviated liver and adipocyte lesions, and inflammation caused by high-fat diets. PNO also significantly decreased atherosclerotic index and ameliorate serum lipid accumulation to prevent cardiovascular disease, which effect the positive control SG group. Moreover, PNO elevated superoxide dismutase and glutathione peroxidase enzyme activities and reduced malondialdehyde levels in the serum. 16S rRNA sequencing showed that PNO restored intestinal microbiota composition, significantly increasing the relative abundance of Lactobacillus and Akkermansia bacteria. These results suggest that Korean pine seeds not only provide adequate fat, protein and energy for squirrels, but also promote physical health and improve body immunity.
文摘Correction to:J.For.Res.https://doi.org/10.1007/s11676-021-01380-2 In the Original publication,the author has found that Table 8 has been inadvertently published with errors due to incorrect calculation.The corrected Table 8 is provided below:Eff ect of PNO supplements on serum lipid levels and the atherosclerosis index TC,TG and LDL-C levels increased,while the HDL-C levels decreased notably in the HFD group compared to the ND group(P<0.01;Table 8).After supplementation with PNO,serum lipid(TG,TC)levels of mice in the M-PNO and H-PNO groups decreased;the diff erences were statistically signifi cant compared with those in the HFD group(P<0.05).Furthermore,PNO supplements signifi-cantly improved HDL-C levels and decreased LDL-C levels(P<0.01).
