Cell metabolism pathways involved in the pathophysiological changes of diabetic peripheral neuropathy

Diabetic peripheral neuropathy is a common complication of diabetes mellitus.Elucidating the pathophysiological metabolic mechanism impels the generation of ideal therapies.However,existing limited treatments for diabetic peripheral neuropathy expose the urgent need for cell metabolism research.Given the lack of comprehensive understanding of energy metabolism changes and related signaling pathways in diabetic peripheral neuropathy,it is essential to explore energy changes and metabolic changes in diabetic peripheral neuropathy to develop suitable treatment methods.This review summarizes the pathophysiological mechanism of diabetic peripheral neuropathy from the perspective of cellular metabolism and the specific interventions for different metabolic pathways to develop effective treatment methods.Various metabolic mechanisms(e.g.,polyol,hexosamine,protein kinase C pathway)are associated with diabetic peripheral neuropathy,and researchers are looking for more effective treatments through these pathways.

Climate Change in Xingtai City in Recent 56 Years and Its Impact on Climate Productivity

The research aimed to understand the impact of climate change on agricultural production in Xingtai City.According to daily rainfall and temperature data in Xingtai City during 1954-2018,change characteristics of climate productivity in Xingtai City were analyzed by using Thornthwaite Memorial model,Mann-Kendall mutation test and linear trend analysis.The results showed that change amplitude of climate productivity with rainfall was obviously larger than that with temperature,and it was affected significantly by rainfall.According to change characteristics and trend of rainfall and temperature,it tended to warm and wet climate in Xingtai City in the future,which was favorable for improvement of crop yield.Additionally,there was still room for greater growth in grain production.

Hepatic steatosis with mass effect:A case report

BACKGROUND Hepatic steatosis is a common radiologic finding.Some imaging inklings are the absence of a mass effect,and there is currently no report of hepatic steatosis with mass effect.CASE SUMMARY A 23-year-old female was admitted due to a liver mass for half a month.No obvious abnormalities were found in physical and laboratory examinations.Ultrasound,computed tomography,and magnetic resonance imaging showed a huge mass between the liver and stomach with a significant mass effect,and the caudate lobe and left lobe of the liver were involved.The signal on T2-and T1-weighted fat-saturated images of the mass was significantly reduced,and the enhanced scan showed inhomogeneous enhancement.Surgical and pathological findings indicated the diagnosis of hepatic steatosis.The operation and re-review of the patient’s images showed that the lesion was supplied by the branch of the hepatic artery.The signal on T1-weighted out-of-phase images of the lesion was lower than on in-phase images,and there was no black rim cancellation artifact around the hepatic steatosis area on T1-weighted out-of-phase images.The dynamic enhancement pattern of the lesion was similar to that of the adjacent normal liver parenchyma.The above characteristics suggested that the lesion was hepatic steatosis.However,in this case,the lesion showed exogenous growth and was mass-like,with an obvious mass effect,which has not been reported previously.CONCLUSION Hepatic steatosis could grow exogenously and has an obvious mass effect.It needs to be distinguished from fat-rich tumors.The T1-weighted in-and out-of-phase images and dynamic enhanced scanning are valuable for differential diagnosis of this lesion.

Eustachian tube teratoma: A case report

BACKGROUND Mature teratoma composed of all three basic germ cell layers of the head and neck is a rare disease.Teratomas involving the temporal bone are particularly scarce.CASE SUMMARY A 48-year-old male patient with a history of chronic otitis of the left ear from infancy,for which he had been operated on twice,was referred to our hospital for chronic otitis,cholesteatoma and a middle ear mass.Computed tomography(CT)scan and magnetic resonance imaging(MRI)revealed a eustachian tube teratoma,in which the anterior lower part and posterior upper part were connected by a thin membranaceous tissue.The mass was removed completely under general anesthesia by mastoidectomy.As of last follow-up(2 years post-surgery),the disease had not relapsed.CONCLUSION Pre-operative CT and MRI are necessary for eustachian tube teratoma.Complete surgical resection provided excellent prognosis.

Graphene oxide coated three-dimensional printed biphasic calcium phosphate scaffold for angiogenic and osteogenic synergy in repairing critical-size bone defect

The custom-tailored medicine requires a developmental strategy that integrates excellent osteogene-sis with mechanical stability to enhance the reconstruction of the critical-size bone defect(CSBD)and the healing process in weight-bearing bone.We prepared three-dimensional(3D)printed biphasic cal-cium phosphate(BCP)scaffolds composited with nano-graphene oxide(GO).The biological effects of the GO/BCP composite scaffolds could induce the differentiation of rat bone marrow stem cells(BM-SCs)and the migration of human umbilical vein endothelial cells(HUVECs)for bone repair.The proper ratio of GO in the composite scaffold regulated the composites’surface roughness and hydrophilicity to a suitable range for the adhesion and proliferation of BMSCs and HUVECs.Besides,the GO/BCP composite scaffold increased osteogenesis and angiogenesis by activating BMP-2,RUNX-2,Smad1/4,and VEGF.The customized intramedullary nail combined with GO/BCP scaffold was applied to repair CSBD(2.0 cm in length)in a beagle femur model.This fixation strategy was confirmed by finite element analysis.In vivo,the results indicated that the custom-made internal fixation provided sufficient stability in the early stage,ensuring bone healing in a considerable mechanical environment.At 9 months postoperatively,longitudi-nal bony union and blood vessels in osteon were observed in the CSBD area with partial degradation in the 0.3%GO/BCP group.In the three-point bending test,the ultimate load of 0.3%GO/BCP group reached over 50%of the normal femur at 9 months after repair.These results showed a promising application of osteogenic GO/BCP scaffold and custom-made intramedullary nails in repairing CSBD of the beagle femur.This effective strategy could provide an option to treat the clinical CSBD in weight-bearing bones.

Non-electric bioelectrical analog strategy by a biophysical-driven nano-micro spatial anisotropic scaffold for regulating stem cell niche and tissue regeneration in a neuronal therapy

The slow regenerating rate and misdirected axonal growth are primary concerns that disturb the curative outcome of peripheral nerve repair.Biophysical intervention through nerve scaffolds can provide efficient,tunable and sustainable guidance for nerve regrowth.Herein,we fabricate the reduced graphene oxide(rGO)/polycaprolactone(PCL)scaffold characterized with anisotropic microfibers and oriented nanogrooves by electrospinning technique.Adipose-derived stem cells(ADSCs)are seeded on the scaffolds in vitro and the viability,neural differentiation efficiency and neurotrophic potential are investigated.RGO/PCL conduits reprogram the phenotype of seeded cells and efficiently repair 15 mm sciatic nerve defect in rats.In summary,biophysical cues on nerve scaffolds are key determinants to stem cell phenotype,and ADSC-seeded rGO/PCL oriented scaffolds are promising,controllable and sustainable approaches to enable peripheral nerve regeneration.

The influence of reduced graphene oxide on stem cells:a perspective in peripheral nerve regeneration

Graphene and its derivatives are fascinating materials for their extraordinary electrochemical and mechanical properties.In recent decades,many researchers explored their applications in tissue engineering and regenerative medicine.Reduced graphene oxide(rGO)possesses remarkable structural and functional resemblance to graphene,although some residual oxygen-containing groups and defects exist in the structure.Such structure holds great potential since the remnantoxygenated groups can further be functionalized or modified.Moreover,oxygen-containing groups can improve the dispersion of rGO in organic or aqueous media.Therefore,it is preferable to utilize rGO in the production of composite materials.The rGO composite scaffolds provide favorable extracellular microenvironment and affect the cellular behavior of cultured cells in the peripheral nerve regeneration.On the one hand,rGO impacts on Schwann cells and neurons which are major components of peripheral nerves.On the other hand,rGO-incorporated composite scaffolds promote the neurogenic differentiation of several stem cells,including embryonic stem cells,mesenchymal stem cells,adipose-derived stem cells and neural stem cells.This review will briefly introduce the production and major properties of rGO,and its potential in modulating the cellular behaviors of specific stem cells.Finally,we present its emerging roles in the production of composite scaffolds for nerve tissue engineering.