Perianal swabs surveillance cultures of Carbapenem-resistant Enterobacteriaceae( CRE) can be hints for CRE bloodstream infection in patients with hematological diseases

Objective To analyze the hints role of surveillance cultures of Carbapenem-resistant Enterobacteriaceae(CRE) by perianal swabs in patients with hematological diseases,and seek risk factors of CRE bloodstream infection.

Analyses of risk factors for intestinal acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation

Objective To investigate the risk factors of intestinal acute graft-versus-host disease(aGVHD)after allogeneic hematopoietic stem cell transplantation(allo-HSCT).Methods The clinical data of 534 cases of 533 patients undergoing allo-HSCT during Jan 2004 and Sep 2012were retrospectively analyzed.The effects of donor-recipient

IL-18 single nucleotide polymorphisms in hematologic malignancies with HLA matched sibling donor allogeneic hematopoietic stem cell transplantation

Objective To explore the impact of interleukin-18(IL-18)single nucleotide polymorphisms on outcomes of hematologic malignancies with HLA-matched sibling donor hematopoietic stem cell transplantation(allo-HSCT).Methods Single-nucleotide polymorphisms in IL-18 promoter was detected by PCR-sequence-specific primer analysis(PCR-SSP)in 93 recipients and their HLA matched sibling donors.Hematopoietic reconstitution,

Prognostic significance of minimal residual disease detection after first induction treatment in adult acute lymphoblastic leukemia patients treated with autologous stem cell transplantation

Objective To investigate the prognostic significanceof detection of minimal residual disease after first inductiontreatment (MRD1) in adult acute lymphoblastic leukemia(ALL) patients treated with autologous stem celltransplantation (auto-HSCT). Methods The clinical dataof 87 ALL patients who underwent auto-HSCT duringFebruary 2006 to April 2017 with MRD1 detection databy flow cytometry were analyzed retrospectively. The relationshipbetween MRD1 and relapse and survival ofALL patients after auto-HSCT was studied. Results Of87 patients,26 (29. 9%) were MRD1 positive. The proportionof high-risk immunophenotype ( pro-B,pro-T,pre-T,mature T) was significantly higher in MRD1-positivepatients than that in MRD1 negative patients(34. 6% vs 14. 5%,P = 0. 038). There was no significantdifference between positive and negative MRD1 patientsof age,sex,lineage ( T /B),immunophenotype(standard risk /high risk),high white blood cell count(B-ALL > 30 × 109 /L or T-ALL > 100 × 109 /L),highriskchromosome /gene ratio,the time from first completeremission to transplantation and pre-treatment regimen.

Analysis of the efficacy and prognosis on first-line autologous hematopoietic stem cell transplantation of patients with multiple myeloma

Objective To explore the efficacy and prognosis of first-line autologous hematopoietic stem cell transplantation(ASCT) for newly diagnosed patients with multiple myeloma(MM).Methods From January 2005

A single-center,randomized controlled trial of PEG-rh G-CSF and common rh G-CSF to promote neutrophil recovery after induction chemotherapy in newly diagnosed acute myeloid leukemia

Objective To compare the time of the recovery of neutrophils or leukocytes by pegylated recombinant human granulocyte stimulating factor(PEG-rhG-CSF)or common recombinant human granulocyte stimulating factor(rhG-CSF)in the myelosuppressive phase after induction chemotherapy in newly diagnosed acute myeloid leukemia(AML)patients.At the same time,the incidences of infection and hospitalization were compared.Methods A prospective randomized controlled trial was conducted in patients with newly diagnosed AML who met the enrollment criteria from August 2014 to December 2017.The patients were randomly divided into two groups according to a 1∶1 ratio:PEG-rhG-CSF group and rhG-CSF group.The time of neutrophil or leukocyte recovery,infection rate and hospitalization interval were compared between the two groups.Results 60 patients with newly diagnosed AML were enrolled:30 patients in the PEG-rhG-CSF group and 30 patients in the rhG-CSF group.There were no significant differences in age,chemotherapy regimen,pre-chemotherapy ANC,WBC,and induction efficacy between the two groups(P>0.05).The median time(range)of ANC or WBC recovery in patients with PEG-rhG-CSF and rhG-CSF were 19(14-35)d and 19(15-26)d,respectively,with no statistical difference(P=0.566).The incidences of infection in the PEG-rhG-CSF group and the rhG-CSF group were 90.0%and 93.3%,respectively,and there was no statistical difference(P=1.000).The median days of hospitalization(range)was 20.5(17-49)days and 21(19-43)days,respectively,with no statistical difference(P=0.530).Conclusion In AML patients after induction therapy,there was no significant difference between the application of PEG-rhG-CSF and daily rhG-CSF in ANC or WBC recovery time,infection incidence and hospitalization time.

Clinical profile of autoimmune hemolytic anemia with monoclonal gammopathy IgMk

Objective To explore the clinical features of autoimmune hemolytic anemia(AIHA)with monoclonal gammopathy IgMk.Methods The clinical and laboratory features of 12 AIHA with monoclonal gammopathy Ig Mκwere retrospectively analyzed.Results 12 cases with monoclonal immunoglobulin Igmκwere found in 85 pa-

The role of bone marrow pathology in diagnosis and differential diagnosis of refractory cytopenia of children

Objective To explore the diagnosis and differential diagnosis of refractory cytopenia of children(RCC) according to WHO classification,and discuss the relationship between the cytology reviewed by hematologists and histology reviewed by pathologists.

Ruxolitinib combined with prednisone,thalidomide and danazol for treatment of myelofibrosis:a pilot study

Objective To evaluate the efficacy and tolerabilityof ruxolitinib combined with prednisone,thalidomide anddanazol for treatment of in myelofibrosis (MF). MethodsPatients of MF according to the WHO 2016 criteria,received ruxolitinib (RUX) combined with prednisone,thalidomide and danazol (PTD). The response,changesof blood counts and adverse events were evaluated. ResultsSix PMF and one post-ET MF patients were enrolled.Four patients presented JAK2V617F mutation,one CALR mutation,one MPL mutation,one triple-negative .Responses per IWG-MRT criteria were clinical improvementin 5 patients,stable disease in 2 ones,spleenresponse in 6 ones. All of 7 patients were symptomaticresponses,four patients achieved at least 50% improvementfrom baseline on MPN-SAF TSS. Three patients initiallywere treated with RUX alone,all of 3 patients experiencedtreatment-associated anemia and thrombocytopenia.

A clinical study of acute lymphoblastic leukemia in adolescents:a single center experience

Objective To explore the clinical and laboratory characteristics,treatment,prognostic factors of acute lymphoblastic leukemia(ALL)in adolescents.Methods Adolescents de novo ALL patients in Blood Disease Hospital,Peking Union Medical College,Chinese Academy of Medical Sciences from September 1999 to September2013 were enrolled in this study.Clinical data,thera-

Role of IFN-γ+874 genetic polymorphisms in allogeneic hematopoietic stem cell transplantation

Objective To explore the impact of IFN-γ+874 polymorphisms on the outcome in HLA matched sibling HSCT. Methods We used PCR-sequence-specific primer analysis(PCR-SSP) to analyze the polymorphisms of IFN-γ+874 T/A in 80 recipient and donor pairs

Clinical and genetic features of dyskeratosis congenital with bone marrow failure in eight patients

Objective To summarize clinical and genetic features of childhood dyskeratosis congenital(DC)patients with bone marrow failure.Methods The clinical data of 8 DC patients with bone marrow failure diagnosed between September 2010 and September 2015 were collected.Whole exons with flanking regions of the 16

A study of clinical characteristics and prognosis of primary myelofibrosis patients with thrombocytopenia in varied degrees

Objective To evaluate clinical characteristics and prognosis of primary myelofibrosis (PMF) patients with thrombocytopenia in varied degrees. Methods Clinical features and survival data of 1 305 Chinese patients with PMF were retrospectively analyzed. The prognostic value of thrombocytopenia in patients with PMF was evaluated.

Clinical outcome of autologous stem cell transplantation as first-line treatment in 30 patients with high risk lymphoblastic lymphoma

Objective To investigate the treatment outcomes of autologous stem cell transplantation(ASCT)as first-line treatment in patients with high risk lymphoblastic lymphoma(LBL)and compare the effect of different induction regimen on prognosis.Methods Thirty LBL patients in complete remission received ASCT from 1996 to 2012in our hospital were retrospectively analyzed.

Homoharringtonine in newly diagnosed acute promyelocytic leukemia treatment: a prospective,randomized controlled trial

Objective To compare the efficacy and toxicities of combining homoharringtonine(HHT)±daunorubicin(DNR)with all-transretinoic acid(ATRA)based therapy and DNR plus ATRA based therapy in newly diagnosed low/intermediate risk acute promyelocytic leukemia(APL).Methods A total of 96 newly diagnosed pa-

Clinical feature and efficacy of patients with multiple myeloma and renal impairment treated with bortezomib based chemotherapy

Objective To explore the efficacy and safety of bortezomib(btz) based chemotherapy in multiple myeloma(MM) patients with renal-function impairment(RI).Methods Fifty-six MM patients with impaired renal

Comparison of efficacy and safety of two different dose of recombinant human thrombopoietin regimens in severe aplastic anemia patients with immunosuppressive therapy

Objective To compare short-term therapeutic effect of different regimens combining recombinant human thrombopoietin(rh TPO)with immunosuppressive therapy(IST)applied in severe aplastic anemia(SAA).Methods The clinical data of newly diagnosed adult SAA patients treated with first-line IST,including 18

Study of nucleophosmin (NPM) gene mutation in patients with acute myeloid leukemia and myelodysplastic syndromes

Objective To investigate nucleophosmin (NPM) gene mutations in patients with de novo acute myeloid leukemia (AML) with normal cytogenetics and primary myelodysplastic syndromes (MDS). Methods Genomic DNA corresponding to exon 12 of NPM gene was amplified by polymerase chain reaction (PCR) in 40 AML patients (28 case untreated and 12 in first remission)

Treatment of transfusion-dependent nonsevere aplastic anemia with cyclosporine A plus ATG/ALG versus cyclosporine A plus androgens: a retrospective single center study

Objective To determine whether cyclosporine A(CsA)plus andronges was as effective as the current standard immunosuppressive therapy(IST)for transfusion-dependent nonsevere aplastic anemia(TD-NSAA).

Cytogentic and prognostic characteristic of acute myeloid leukemia with monosomal karyotype

Objective To explore the cytogenetic and prognostic significance of monosomal karyotype(MK)in adult patients with acute myeloid leukemia(AML).Methods From September 2002 to Nevember 2014 in Blood Diseases Hospital,Chinese Academy of Medical Sciences,97Cases with AML were enrolled,including 96 cases