Collagen organization plays an important role in maintaining structural integrity and determining tissue function.Polarization-sensitive optical coherence tomography(PSOCT)is a promising noninvasive three-dimensional ...Collagen organization plays an important role in maintaining structural integrity and determining tissue function.Polarization-sensitive optical coherence tomography(PSOCT)is a promising noninvasive three-dimensional imaging tool for mapping collagen organization in vivo.While PSOCT systems with multiple polarization inputs have demonstrated the ability to visualize depth-resolved collagen organization,systems,which use a single input polarization state have not yet demonstrated sufficient reconstruction quality.Herein we describe a PSOCT based polarization state transmission model that reveals the depth-dependent polarization state evolution of light backscattered within a birefringent sample.Based on this model,we propose a polarization state tracing method that relies on a discrete differential geometric analysis of the evolution of the polarization state in depth along the Poincare sphere for depth-resolved birefringent imaging using only one single input polarization state.We demonstrate the ability of this method to visualize depth-resolved myocardial architecture in both healthy and infarcted rodent hearts(ex vivo)and collagen structures responsible for skin tension lines at various anatomical locations on the face of a healthy human volunteer(in vivo).展开更多
基金Research supported by grants from the National Institutes of Health(R01EY028753,R01HL141570,R01AR077560)Washington Research Foundation,an unrestricted grant from the Research to Prevent Blindness,Inc.,New York,NY.M.Kirby was supported by an NSF graduate fellowship(No.DGE-1256082)The funding organization had no role in the design or conduct of this research.
文摘Collagen organization plays an important role in maintaining structural integrity and determining tissue function.Polarization-sensitive optical coherence tomography(PSOCT)is a promising noninvasive three-dimensional imaging tool for mapping collagen organization in vivo.While PSOCT systems with multiple polarization inputs have demonstrated the ability to visualize depth-resolved collagen organization,systems,which use a single input polarization state have not yet demonstrated sufficient reconstruction quality.Herein we describe a PSOCT based polarization state transmission model that reveals the depth-dependent polarization state evolution of light backscattered within a birefringent sample.Based on this model,we propose a polarization state tracing method that relies on a discrete differential geometric analysis of the evolution of the polarization state in depth along the Poincare sphere for depth-resolved birefringent imaging using only one single input polarization state.We demonstrate the ability of this method to visualize depth-resolved myocardial architecture in both healthy and infarcted rodent hearts(ex vivo)and collagen structures responsible for skin tension lines at various anatomical locations on the face of a healthy human volunteer(in vivo).
