Massively parallel sequencing （MPS） technology is capable of determining the sizes of short tandem repeat （STR） alleles as well as their individual nueleotide sequences. Thus, single nucleotide polymorphisms （SNP...Massively parallel sequencing （MPS） technology is capable of determining the sizes of short tandem repeat （STR） alleles as well as their individual nueleotide sequences. Thus, single nucleotide polymorphisms （SNPs） within the repeat regions of STRs and variations in the pattern of repeat units in a given repeat motif can be used to differentiate alleles of the same length. In this study, MPS was used to sequence 28 forensically-relevant Y-chromosome STRs in a set of 41 DNA samples from the 3 major U.S. population groups （African Americans, Caucasians, and Hispanics）. The resulting sequence data, which were analyzed with STRait Razor v2.0, revealed 37 unique allele sequence variants that have not been previously reported. Of these, 19 sequences were variations of documented sequences resulting from the presence of intra-repeat SNPs or alternative repeat unit patterns. Despite a limited sampling, two of the most frequently-observed variants were found only in African American samples. The remaining 18 variants represented allele sequences for which there were no published data with which to compare. These findings illustrate the great potential of MPS with regard to increasing the resolving power of STR typing and emphasize the need for sample population characterization of STR alleles.展开更多
All ceiis show some degree of poiarity, either by asymmetrically distributed membrane or cytosolic components. Even in bacterial cells that do not have the eukaryotic membrane compartmentalization of the cytoplasm, pr...All ceiis show some degree of poiarity, either by asymmetrically distributed membrane or cytosolic components. Even in bacterial cells that do not have the eukaryotic membrane compartmentalization of the cytoplasm, proteins can be localized at specific areas. In rod-shaped bacteria, many processes such as signaling, flagella formation, and DNA uptake occur at the cell poles. In addition,展开更多
基金supported in part by the grant‘‘Development of Reference Sample DNA Profiling for Databases Using Next Generation Sequencing Technologies"(Award No.2012-DNBXK033)awarded to BB by the National Institute of Justice,Office of Justice Programs,U.S
文摘Massively parallel sequencing （MPS） technology is capable of determining the sizes of short tandem repeat （STR） alleles as well as their individual nueleotide sequences. Thus, single nucleotide polymorphisms （SNPs） within the repeat regions of STRs and variations in the pattern of repeat units in a given repeat motif can be used to differentiate alleles of the same length. In this study, MPS was used to sequence 28 forensically-relevant Y-chromosome STRs in a set of 41 DNA samples from the 3 major U.S. population groups （African Americans, Caucasians, and Hispanics）. The resulting sequence data, which were analyzed with STRait Razor v2.0, revealed 37 unique allele sequence variants that have not been previously reported. Of these, 19 sequences were variations of documented sequences resulting from the presence of intra-repeat SNPs or alternative repeat unit patterns. Despite a limited sampling, two of the most frequently-observed variants were found only in African American samples. The remaining 18 variants represented allele sequences for which there were no published data with which to compare. These findings illustrate the great potential of MPS with regard to increasing the resolving power of STR typing and emphasize the need for sample population characterization of STR alleles.
文摘All ceiis show some degree of poiarity, either by asymmetrically distributed membrane or cytosolic components. Even in bacterial cells that do not have the eukaryotic membrane compartmentalization of the cytoplasm, proteins can be localized at specific areas. In rod-shaped bacteria, many processes such as signaling, flagella formation, and DNA uptake occur at the cell poles. In addition,
