In this paper, toxicity and safety of high-dose irradiated chicken-breast meat were evaluated. For assays of acute toxicity, genetic toxicity, and sub-chronic toxicity, ames test, mice bone marrow erythrocyte micronuc...In this paper, toxicity and safety of high-dose irradiated chicken-breast meat were evaluated. For assays of acute toxicity, genetic toxicity, and sub-chronic toxicity, ames test, mice bone marrow erythrocyte micronucleus, and mice sperm abnormality were performed. The results showed that, in the acute oral toxicity tests, median lethal dose （more than 10 000 mg kg-~） in male and female ICR mice showed no toxicological signs. For subacute 30-d oral toxicology of irradiated chicken-breast meat with dose of 10, 15 and 25 kGy in both male and female SD rats, no noticeable toxicological effects were observed. It is concluded that chicken-breast meat with high-dose irradiation has no acute toxicity and no genotoxicity, nor harmful effects on the animal body at the tested dosage range. Therefore, high-dose irradiated chicken-breast meat is safe for pet consumption.展开更多
A kind of alfalfa seeds was irradiated by 1, 2, 3, 4 and 5 kGy at a dose rate of 6.288 kGy·h-1 in a self-shielded irradiator of 137Cs gamma rays. The EPR spectra, which were measured subsequently between 0.3401 a...A kind of alfalfa seeds was irradiated by 1, 2, 3, 4 and 5 kGy at a dose rate of 6.288 kGy·h-1 in a self-shielded irradiator of 137Cs gamma rays. The EPR spectra, which were measured subsequently between 0.3401 and 0.3501 T, showed that there was a direct proportional relationship between the EPR signal strength of free radi- cals produced by gamma irradiation in the alfalfa seeds and absorbed dose. The first derivative EPR spectra of the al- falfa seeds were very clear and easy to identify. However, the EPR signal strength of the peak-to-peak amplitude de- creased rapidly and most of them decayed beyond 50% within 3 days after the seeds were irradiated. It tended to sta- bilize after half a month since the seeds were irradiated. The differences of the EPR signal strength between the irra- diated and unirradiated alfalfa seeds still remained. All seeds were stored at ambient temperature for more than 3 months. Therefore, using EPR spectrometry technique to measure free radicals in alfalfa seeds as a means to deter- mine whether the seeds have been irradiated or not is feasible, relatively fast and simple.展开更多
Wheat yellow mosaic is a soil-borne virus disease,and now has become one of the main viral diseases endangering wheat production in China,posing serious threat to wheat production. The pathogen,transmission media,inci...Wheat yellow mosaic is a soil-borne virus disease,and now has become one of the main viral diseases endangering wheat production in China,posing serious threat to wheat production. The pathogen,transmission media,incidence regularity and performance of wheat yellow mosaic were introduced in the paper,and its molecular biology and breeding research were summarized,in order to provide a reference for resistance breeding and production practice of wheat yellow mosaic.展开更多
In recent years, the promoter of Rhizobium nodulation gene has been studiedincreasingly. It is well known that the promoter of rhizobia nodulation genes is in-duced by plant inducer flavonoid. The transcription of nod...In recent years, the promoter of Rhizobium nodulation gene has been studiedincreasingly. It is well known that the promoter of rhizobia nodulation genes is in-duced by plant inducer flavonoid. The transcription of nodulation genes atdownstream begins, when the NodD protein is present. This kind of promoters isvery conservative in structure, always containing nod boxes. In previous reports, the Azorhizobium caulinodans ORS571 as Rhizobium has beenshown to harbour nodABC-related genes (nod locus 1) and nodD-related gene(nodlocus 3). Recently, the details of the identification of a new nod locus 5 gene展开更多
基金supported by the Special Fund for Agro-Scientific Research in the Public Interest, China(201103007)
文摘In this paper, toxicity and safety of high-dose irradiated chicken-breast meat were evaluated. For assays of acute toxicity, genetic toxicity, and sub-chronic toxicity, ames test, mice bone marrow erythrocyte micronucleus, and mice sperm abnormality were performed. The results showed that, in the acute oral toxicity tests, median lethal dose （more than 10 000 mg kg-~） in male and female ICR mice showed no toxicological signs. For subacute 30-d oral toxicology of irradiated chicken-breast meat with dose of 10, 15 and 25 kGy in both male and female SD rats, no noticeable toxicological effects were observed. It is concluded that chicken-breast meat with high-dose irradiation has no acute toxicity and no genotoxicity, nor harmful effects on the animal body at the tested dosage range. Therefore, high-dose irradiated chicken-breast meat is safe for pet consumption.
文摘A kind of alfalfa seeds was irradiated by 1, 2, 3, 4 and 5 kGy at a dose rate of 6.288 kGy·h-1 in a self-shielded irradiator of 137Cs gamma rays. The EPR spectra, which were measured subsequently between 0.3401 and 0.3501 T, showed that there was a direct proportional relationship between the EPR signal strength of free radi- cals produced by gamma irradiation in the alfalfa seeds and absorbed dose. The first derivative EPR spectra of the al- falfa seeds were very clear and easy to identify. However, the EPR signal strength of the peak-to-peak amplitude de- creased rapidly and most of them decayed beyond 50% within 3 days after the seeds were irradiated. It tended to sta- bilize after half a month since the seeds were irradiated. The differences of the EPR signal strength between the irra- diated and unirradiated alfalfa seeds still remained. All seeds were stored at ambient temperature for more than 3 months. Therefore, using EPR spectrometry technique to measure free radicals in alfalfa seeds as a means to deter- mine whether the seeds have been irradiated or not is feasible, relatively fast and simple.
基金Supported by Major Technology Project of Independent Innovation in Shandong Province ( 2014GJJS0201)Major Scientific and Technological Achievements Cultivation Project of Shandong Academy of Agricultural Sciences( 2014CGPY03)+2 种基金National Key Technology R&D Program ( 2014BAA03B042013BAD01B02)Special Fund for National Scientific Research in the Public Interest ( 201303007-1)
文摘Wheat yellow mosaic is a soil-borne virus disease,and now has become one of the main viral diseases endangering wheat production in China,posing serious threat to wheat production. The pathogen,transmission media,incidence regularity and performance of wheat yellow mosaic were introduced in the paper,and its molecular biology and breeding research were summarized,in order to provide a reference for resistance breeding and production practice of wheat yellow mosaic.
文摘In recent years, the promoter of Rhizobium nodulation gene has been studiedincreasingly. It is well known that the promoter of rhizobia nodulation genes is in-duced by plant inducer flavonoid. The transcription of nodulation genes atdownstream begins, when the NodD protein is present. This kind of promoters isvery conservative in structure, always containing nod boxes. In previous reports, the Azorhizobium caulinodans ORS571 as Rhizobium has beenshown to harbour nodABC-related genes (nod locus 1) and nodD-related gene(nodlocus 3). Recently, the details of the identification of a new nod locus 5 gene
