The development of laminin-alginate microspheres encapsulated with Ginsenoside Rg1 and ADSCs for breast reconstruction after lumpectomy

Many technologies have been developed for breast reconstruction after lumpectomy.Although the technologies achieved promising success in clinical,there are still many shortages hanging over and trouble the researchers.Tissue engineering technology was introduced to plastic surgery that gave a light to lumpectomy patients in breast reconstruction.The unexpected absorption rate,resulting from limited vascularization and low cell survival rate,is a major factor that leads to unsatisfactory results for the previous studies in our lab.In the study,the laminin-modified alginate synthesized by a new method of low concertation of sodium periodate would be mixed with ADSCs and Rg1 in the medium;and then sprayed into a calcium chloride(CaCl2)solution to prepare into microsphere(abbreviated as ADSC-G-LAMS)by bio-electrospray with a power syringe for the mass production and smaller bead size.The developed ADSC-G-LAMS microspheres had the diameter of 232±42μm.Sustained-release of the Rg1 retained its biological activity.WST-1,live/dead staining,and chromosome aberration assay were evaluated to confirm the safety of the microspheres.In in vivo study,ADSC-G-LAMS microspheres combined with autologous adipocytes were transplanted into the dorsum of rats by subcutaneous injection.The efficacy was investigated by H&E and immunofluorescence staining.The results showed that the bioactive ADSC-G-LAMS microspheres could integrate well into the host adipose tissue with an adequate rate of angiogenesis by constantly releasing Rg1 to enhance the ADSC or adipocyte survival rate to join tissue growth and repair with adipogenesis for breast reconstruction after lumpectomy.

Aptamerized silica/gold nanocapsules for stimulated release of doxorubicin through remote two-photon excitation

Precision-based drug delivery via remote triggering is fast becoming an attractive therapeutic design and is highly useful in complicated clinical situations that may require accurate site-delivery of drug while reducing the risk of collateral damage to surrounding healthy tissue.Of the many strategies available to achieve these desirable effects,silica/gold nano-assemblies offers a practical means to achieving these aims.Herein,as a proof-of-concept,a silica nanocapsule passivated with a gold outer nanoshell had been fabricated to deliver Doxorubicin,and this nano-assembly can be remotely triggered via two-photon excitation(TPE),even under in vivo setting.A polyethylene glycol(PEG)layer as well as AS1411 DNA aptamer had also been grafted to the surface to improve homing specificity toward MDA-MB-231 breast cancer tissue.The assembly of silica/gold nanocapsules was characterized via TEM,FTIR,and UVVis to validate the the nanoconstruct.Upon TPE irradiation,a higher expression level of Annexin V and Caspase-3 was observed in both in vitro and in vivo animal models.A significant reduction in tumor size on mice model was noticed after 21 days,and these results had suggested a viable nano-sized design serving as remotely triggered drug release platform based on current well-established silica nanoparticulate methodologies.

New design to remove leukocytes from platelet-rich plasma(PRP)based on cell dimension rather than density

Platelet-rich plasma(PRP)can stimulate the proliferation of stem cells and have a positive effect on tissue repair.Although many commercialized PRP preparation kits are already on the market,first-line clinical workers are still not satisfied with most of the PRP kits.The work of commercial PRP kits is based on the density of blood elements.However,the blood elements are very close in density which makes the separation challenging.Therefore,the mentioned commercialized kits are generally contaminated by leucocytes and erythrocyte.In this study,a home-designed PRP device was developed to use a separation membrane with adequate cut-off pore size of 5μm,3μm and 2μm for the groups of H5M,H3M,and H2M,respectively,to be placed in the middle of the centrifuge tube.The home-designed H2M showed a very promising results regardless of the final volume(1.82±0.09 ml),platelet yield(8.39±0.44%),Red Blood Cells(0%),White Blood Cells(0%),and Relative Concentration of Platelet Increment value(225.09%).Further,it showed a good result in cell viability and cytotoxicity and confirmed a good multilineage potentials.The concentration in PRP prepared by group H2M was relatively stable and far above average.All the fibrin fibers were linked together as bridging strands or strings and turned into an inter-connected porous structure for nutrients transportation and regenerative cell migration.We believe that the home-designed group H2M should have a great potential to develop into the final product to meet the requirements of first-line clinical workers.