The significance of bioink suitability for the extrusion bioprinting of tissue-like constructs cannot be overemphasized.Gelatin,derived from the hydrolysis of collagen,not only can mimic the extracellular matrix to imm...The significance of bioink suitability for the extrusion bioprinting of tissue-like constructs cannot be overemphasized.Gelatin,derived from the hydrolysis of collagen,not only can mimic the extracellular matrix to immensely support cell function,but also is suitable for extrusion under certain conditions.Thus,gelatin has been recognized as a promising bioink for extrusion bioprinting.However,the development of a gelatin-based bioink with satisfactory printability and bioactivity to fabricate complex tissue-like constructs with the desired physicochemical properties and biofunctions for a specific biomedical application is still in its infancy.Therefore,in this review,we aim to comprehensively summarize the state-of-the-art methods of gelatin-based bioink application for extrusion bioprinting.Wefirstly outline the properties and requirements of gelatin-based bioinks for extrusion bioprinting,highlighting the strategies to overcome their main limitations in terms of printability,structural stability and cell viability.Then,the challenges and prospects are further discussed regarding the development of ideal gelatin-based bioinks for extrusion bioprinting to create complex tissue-like constructs with preferable physicochemical properties and biofunctions.展开更多
BACKGROUND Postoperative atrial fibrillation(POAF)is a common yet understudied clinical issue after coronary artery bypass graft(CABG)leading to higher mortality rates and stroke.This systematic review and metaanalysi...BACKGROUND Postoperative atrial fibrillation(POAF)is a common yet understudied clinical issue after coronary artery bypass graft(CABG)leading to higher mortality rates and stroke.This systematic review and metaanalysis evaluated the rates of adverse outcomes between patients with and without POAF in patients treated with CABG or combined procedures.METHODS The search period was from the beginning of PubMed and Embase to May 18th,2020 with no language restrictions.The inclusion criteria were:(1)studies comparing new onset atrial fibrillation before or after revascularization vs.no new onset AF before or after revascularization.The outcomes assessed included allcause mortality,cardiac death,cerebral vascular accident(CVA),myocardial infarction(MI),repeated revascularization,major adverse cardiac event(MACE),and major adverse cardiac and cerebrovascular events(MACCEs).RESULTS Of the 7,279 entries screened,11 studies comprising of 57,384 patients were included.Compared to nonPOAF,POAF was significantly associated with higher risk of allcause mortality(Risk Ratio(RR)=1.58;95%Confidence Interval(CI):1.42−1.76,P<0.00001)with accompanying high level of heterogeneity(I^(2)=62%).Conclusions Patients with POAF after CABG or combined procedures are at an increased risk of allcause mortality or CVAs.Therefore,POAF after such procedures should be closely monitored and treated judiciously to minimize risk of further complications.While there are studies on POAF versus no POAF on outcomes,the heterogeneity suggests that further studies are needed.展开更多
Numerous small-molecule amines(SMAs)play critical roles in maintaining bone homeostasis and promoting bone regeneration regardless of whether they are applied as drugs or biomaterials.On the one hand,SMAs promote bone...Numerous small-molecule amines(SMAs)play critical roles in maintaining bone homeostasis and promoting bone regeneration regardless of whether they are applied as drugs or biomaterials.On the one hand,SMAs promote bone formation or inhibit bone resorption through the regulation of key molecular signaling pathways in osteoblasts/osteoclasts;on the other hand,owing to their alkaline properties as well as their antioxidant and anti-inflammatory features,most SMAs create a favorable microenvironment for bone homeostasis.However,due to a lack of information on their structure/bioactivity and underlying mechanisms of action,certain SMAs cannot be developed into drugs or biomaterials for bone disease treatment.In this review,we thoroughly summarize the current understanding of SMA effects on bone homeostasis,including descriptions of their classifications,biochemical features,recent research advances in bone biology and related regulatory mechanisms in bone regeneration.In addition,we discuss the challenges and prospects of SMA translational research.展开更多
Tumor vascular dysfunction and immune suppression predict poor outcomes of tumor therapy.Combination of photothermal therapy(PTT)and vessel normalization with tumor immunotherapy is promising to augment antitumor bene...Tumor vascular dysfunction and immune suppression predict poor outcomes of tumor therapy.Combination of photothermal therapy(PTT)and vessel normalization with tumor immunotherapy is promising to augment antitumor benefit.Herein,we develop a potential immunostimulatory nanomodulator for treatment of triple-negative breast cancer(TNBC)treatment via synergism of PTT,vessel normalization,and priming of tumoral suppressive immune microenvironment by blocking transforming growth factor-β(TGF-β)pathway.The nanomodulator,namely Vac@Apt@BPs,is developed by conjugation of TGF-βinhibitor Vactosertib(Vac)and nucleolin-recognizing aptamer(Apt)on the surface of black phosphorus nanoparticles(BPs).Vac@Apt@BPs show good accumulation in TNBC via aptamer-induced active targeting of TNBC.Via the blockade of TGF-βsignaling,Vac@Apt@BPs effectively inhibit the formation of tumor neovascular,and normalize the vessels to recover vascular integrity and alleviate the hypoxia stress.Together with the tumor eradication and immunogenic cell death via PTT,robust immune response was boosted by promoted maturation of dendritic cells,suppression of regulatory T cells,and stimulation of effective T cells.This synergistic therapeutic strategy potentially suppresses the growth of TNBC in mice.展开更多
Integration of two or more biomolecules with synergetic and complementary effects on a material surface can help to obtain multi-functions for various biomedical applications.However,the amounts of biomolecules integr...Integration of two or more biomolecules with synergetic and complementary effects on a material surface can help to obtain multi-functions for various biomedical applications.However,the amounts of biomolecules integrated and their physiological functions are compromised due to the limited surface anchoring sites.Herein,we propose a novel concept of film engineering strategy“from surface to bulk synergetic modification”.This new concept is realized by employing the surface amine groups of plasma polymerized allylamine(PPAm)film for grafting a molecule e.g.,thrombin inhibitor,bivalirudin(BVLD),meanwhile its bulk amine groups is used as a universal depot for storing and releasing therapeutic nitric oxide(NO)gas as supplement to the functions of BVLD.It is demonstrated that such a“from surface to bulk synergetic modification”film engineering can impart the modified-substrates with anti-platelet and anti-coagulant dual functions,giving rise to a highly endotheliummimetic thromboresistant property.We believe that our research provides a very promising strategy to deliver multifunctional surface versatilely that require NO release in combination with other properties,which will find broad biomedical applications in blood-contacting devices,and et al.Moreover,it also provides a brand-new film engineering strategy for tailoring surface multi-functionalities of a wide range of materials.展开更多
Low patency ratio of small-diameter vascular grafts remains a major challenge due to the occurrence of thrombosis formation and intimal hyperplasia after transplantation.Although developing the functional coating with...Low patency ratio of small-diameter vascular grafts remains a major challenge due to the occurrence of thrombosis formation and intimal hyperplasia after transplantation.Although developing the functional coating with release of bioactive molecules on the surface of small-diameter vascular grafts are reported as an effective strategy to improve their patency ratios,it is still difficult for current functional coatings cooperating with spatiotemporal control of bioactive molecules release to mimic the sequential requirements for antithrombogenicity and endothelialization.Herein,on basis of 3D-printed polyelectrolyte-based vascular grafts,a biologically inspired release system with sequential release in spatiotemporal coordination of dual molecules through an electrostatic self-assembly was first described.A series of tubes with tunable diameters were initially fabricated by a coaxial extrusion printing method with customized nozzles,in which a polyelectrolyte ink containing of ε-polylysine and sodium alginate was used.Further,dual bioactive molecules,heparin with negative charges and Tyr-Ile-Gly-Ser-Arg(YIGSR)peptide with positive charges were layer-by-layer assembled onto the surface of these 3D-printed tubes.Due to the electrostatic interaction,the sequential release of heparin and YIGSR was demonstrated and could construct a dynamic microenvironment that was thus conducive to the antithrombogenicity and endothelialization.This study opens a new avenue to fabricate a small-diameter vascular graft with a biologically inspired release system based on electrostatic interaction,revealing a huge potential for development of small-diameter artificial vascular grafts with good patency.展开更多
Gold nanoparticles(AuNPs) have been attractive for nanomedicine because of their pronounced optical properties.Here,we customerized the methods to synthesize two types of gold nanostars,Au nanostars-1 and Au nanostars...Gold nanoparticles(AuNPs) have been attractive for nanomedicine because of their pronounced optical properties.Here,we customerized the methods to synthesize two types of gold nanostars,Au nanostars-1 and Au nanostars-2,which have different spire lengths and optical properties,and also spherical AuNPs.Compared to nanospheres,gold nanostars were less toxic to a variety of cells,including macrophages.Au nanostars-1 and Au nanostars-2 also manifested a similar pattern of tissue distribution upon in vivo administration in mice to that of nanospheres,and but reveled less liver retention than nanospheres.Due to their strong absorption in the near-infrared(NIR),Au nanostars-2 induced a strong hyperthermia effect in vitro upon excitation at 808 nm,and elicited a robust photothermal therapy(PTT) efficacy in ablating tumors in a mouse model of orthotopic breast cancer using 4T1 breast cancer cells.Meanwhile,Au nanostars-1 showed a great capability to enhance the Raman signal through surface-enhanced Raman spectroscopy(SERS) in 4T1 cells.Our combined results opened a new avenue to develop Au nanostars for cancer imaging and therapy.展开更多
The effect of particle shape on the porosity and compressive strength of porous hydroxyapatite(HA)scaffolds was investigated by sintering the mixture of rod-shaped HA(r-HA) and spherical HA(s-HA)with polyacrylamide us...The effect of particle shape on the porosity and compressive strength of porous hydroxyapatite(HA)scaffolds was investigated by sintering the mixture of rod-shaped HA(r-HA) and spherical HA(s-HA)with polyacrylamide used as the sacrificial template. It was found, for the first time, that addition of r-HA into s-HA could exponentially decrease the porosity of sintered HA scaffolds and enhance their compressive strength with the increase of r-HA content. The mechanism, according to the results from scanning electron microscopy and X-ray diffraction, lies in the restriction of s-HA to the grain formation and growth of r-HA during sintering and results in the fusion of r-HA with s-HA. These findings suggest that mixture of r-HA and s-HA might provide a new and facile way to improve the compressive strength of porous HA scaffolds.展开更多
Elasticity is a fundamental mechanical property of two-dimensional(2D)materials,and is critical for their application as well as for strain engineering.However,accurate measurement of the elastic modulus of 2D materia...Elasticity is a fundamental mechanical property of two-dimensional(2D)materials,and is critical for their application as well as for strain engineering.However,accurate measurement of the elastic modulus of 2D materials remains a challenge,and the conventional suspension method suffers from a number of drawbacks.In this work,we demonstrate a method to map the in-plane Young’s modulus of mono-and bi-layer MoS_(2) on a substrate with high spatial resolution.Bimodal atomic force microscopy is used to accurately map the effective spring constant between the microscope tip and sample,and a finite element method is developed to quantitatively account for the effect of substrate stiffness on deformation.Using these methods,the in-plane Young’s modulus of monolayer MoS_(2) can be decoupled from the substrate and determined as 265±13 GPa,broadly consistent with previous reports though with substantially smaller uncertainty.It is also found that the elasticity of mono-and bi-layer MoS_(2) cannot be differentiated,which is confirmed by the first principles calculations.This method provides a convenient,robust and accurate means to map the in-plane Young’s modulus of 2D materials on a substrate.展开更多
Cancer has become the leading cause of death.The progress in diagnosis and treatment is still limited.Over the past three decades,emergence and rapid development of nanotechnology have brought new hopes for cancer the...Cancer has become the leading cause of death.The progress in diagnosis and treatment is still limited.Over the past three decades,emergence and rapid development of nanotechnology have brought new hopes for cancer therapy.A repertoire of nanomaterials with controllable size-,shape-,and composition-dependent physiochemical proper展开更多
Nanomaterials with strong near-infrared absorption and high photothermal conversion ability have shown great application potential in many areas including cancer treatment.However,the current photothermal conversion n...Nanomaterials with strong near-infrared absorption and high photothermal conversion ability have shown great application potential in many areas including cancer treatment.However,the current photothermal conversion nanomaterials generally have poor biodegradability and would stay in展开更多
In the field of regenerative medicine,the importance of 3D bioprinting is self-evident and nonnegligible.However,3D bioprinting technology also requires bioink with excellent performance as support material to fabrica...In the field of regenerative medicine,the importance of 3D bioprinting is self-evident and nonnegligible.However,3D bioprinting technology also requires bioink with excellent performance as support material to fabricate a multi-functional bioinspired scaffold.Collagen-based bioink is regarded as an ideal 3D bioprinting ink for its excellent biocompatibility,controllable printability and cell loading property.It is an important breakthrough in regenerative medicine with the progress of collagen-based bioink,which fabricates bioinspired scaffolds with different functions and is applied in different repair scenarios.This review summarizes the different applications of collagen-based bioink and classifies them as soft tissue and hard tissue according to the target region.The applications of target region in soft tissues include skin,cartilage,heart and blood vessels,while in hard tissues include femur,skull,teeth and spine.When the collagen-based bioink is applied in repairing soft tissue,the requirements of function are higher,while the mechanical properties must be further improved in repairing hard tissue.We further summarize the characteristics of collagen-based bioink and point out the most important properties that should be considered in different repair scenarios,which can provide reference for the preparation of bioinks with different functions.Finally,we point out the main challenges faced by collagen-based bioink and prospect the future research directions.展开更多
As one of near-infrared(NIR) fluorescent(FL) nanoprobes, gold nanoclusters(Au NCs) are delicated to passive-targeting tumors for NIR FL imaging, but which easily cleared by the kidneys for the small size(<1.5 nm). ...As one of near-infrared(NIR) fluorescent(FL) nanoprobes, gold nanoclusters(Au NCs) are delicated to passive-targeting tumors for NIR FL imaging, but which easily cleared by the kidneys for the small size(<1.5 nm). Herein, the well-defined gold clusters nanoassembly(Au CNA) was synthesized by the selfassembly of Au NCs based on protein cross-linking approach. The as-prepared Au CNA demonstrated highly effective cellular uptake and precise tumor targeting compared to that of Au NCs. Moreover, with the irradiation of 660 nm laser, Au CNA generated largely reactive oxygen species(ROS) for photodynamic therapy(PDT). In vitro and [39_TD$IF]in vivo PDT revealed that Au CNA exhibited largely cell death and significantly tumor removal at a low power density of 0.2 W/cm^2. It could be speculated that the laser-excited Au CNA produced photon energy, which further obtained electron from oxygen to generate radical species.Therefore, Au CNA as a photosensitizer could realize NIR FL imaging and NIR laser induced PDT.展开更多
Spectrin is a large,cytoskeletal,and heterodimeric protein composed of modular structure of and subunits,it typically contains 106 contiguous amino acid sequence motifs called"spectrin repeats".Spectrin is c...Spectrin is a large,cytoskeletal,and heterodimeric protein composed of modular structure of and subunits,it typically contains 106 contiguous amino acid sequence motifs called"spectrin repeats".Spectrin is crucial for maintaining the stability and structure of the cell membrane and the shape of a cell.Moreover,it contributes to diverse cell functions such as cell adhesion,cell spreading,and the cell cycle.Mutations of spectrin lead to various human diseases such as hereditary hemolytic anemia,type 5 spinocerebellar ataxia,cancer,as well as others.This review focuses on recent advances in determining the structure and function of spectrin as well as its role in disease.展开更多
Liver disease has long been a heavy health and economic burden worldwide.Once the disease is out of control and progresses to end-stage or acute organ failure,orthotopic liver transplantation(OLT)is the only therapeut...Liver disease has long been a heavy health and economic burden worldwide.Once the disease is out of control and progresses to end-stage or acute organ failure,orthotopic liver transplantation(OLT)is the only therapeutic alternative,and it requires appropriate donors and aggressive administration of immunosuppressive drugs.Therefore,hepatocyte transplantation(HT)and bioartificial livers(BALs)have been proposed as effective treatments for acute liver failure(ALF)in clinics.Although human primary hepatocytes(PHs)are an ideal cell source to support these methods,the large demand and superior viability of PH is needed,which restrains its wide usage.Thus,a finding alternative to meet the quantity and quality of hepatocytes is urgent.In this context,human pluripotent stem cells(PSC),which have unlimited proliferative and differential potential,derived hepatocytes are a promising renewable cell source.Recent studies of the differentiation of PSC into hepatocytes has provided evidence that supports their clinical application.In this review,we discuss the recent status and future directions of the potential use of PSC-derived hepatocytes in treating ALF.We also discuss opportunities and challenges of how to promote such strategies in the common applications in clinical treatments.展开更多
The recent novel coronavirus disease(COVID-19)outbreak,caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is seeing a rapid increase in infected patients worldwide.The host immune response to SARS-C...The recent novel coronavirus disease(COVID-19)outbreak,caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is seeing a rapid increase in infected patients worldwide.The host immune response to SARS-CoV-2 appears to play a critical role in disease pathogenesis and clinical manifestations.SARS-CoV-2 not only activates antiviral immune responses,but can also cause uncontrolled inflammatory responses characterized by marked pro-inflammatory cytokine release in patients with severe COVID-19,leading to lymphopenia,lymphocyte dysfunction,and granulocyte and monocyte abnormalities.These SARS-CoV-2-induced immune abnormalities may lead to infections by microorganisms,septic shock,and severe multiple organ dysfunction.Therefore,mechanisms underlying immune abnormalities in patients with COVID-19 must be elucidated to guide clinical management of the disease.Moreover,rational management of the immune responses to SARSCoV-2,which includes enhancing anti-viral immunity while inhibiting systemic inflammation,may be key to successful treatment.In this review,we discuss the immunopathology of COVID-19,its potential mechanisms,and clinical implications to aid the development of new therapeutic strategies against COVID-19.展开更多
In osteoporosis scenario, tissue response to implants is greatly impaired by the deteriorated boneregeneration microenvironment. In the present study, a Mg-containing akermanite (Ak) ceramic wasemployed for the treatm...In osteoporosis scenario, tissue response to implants is greatly impaired by the deteriorated boneregeneration microenvironment. In the present study, a Mg-containing akermanite (Ak) ceramic wasemployed for the treatment of osteoporotic bone defect, based on the hypothesis that both beneficialions (e.g. Mg^2+ ect.) released by the implants and the weak alkaline microenvironment pH (μe-pH) itcreated may play distinct roles in recovering the abnormal bone regeneration by stimulating osteoblasticanabolic effects. The performance of Ak, b-tricalcium phosphate (β-TCP) and Hardystone (Har) in healinga 3 mm bone defect on the ovariectomized (OVX) osteoporotic rat model was evaluated. Our resultsindicated that, there's more new bone formed in Ak group than in β-TCP or Har group at week 9. Theinitial me-pHs of Ak were significantly higher than that of the β-TCP and Blank group, and this weakalkaline condition was maintained till at least 9 weeks post-surgery. Increased osteoblastic activity whichwas indicated by higher osteoid secretion was observed in Ak group at week 4 to week 9. An intermediatelayer which was rich in phosphorus minerals and bound directly to the new forming bone wasdeveloped on the surface of Ak. In a summary, our study demonstrates that Ak exhibits a superior boneregenerative performance under osteoporosis condition, and might be a promising candidate for thetreatment of osteoporotic bone defect and fracture.展开更多
Although extrusion-based three-dimensional(EB-3D)printing technique has been widely used in the complex fabrication of bone tissue-engineered scaffolds,a natural bone-like radial-gradient scaffold by this processing m...Although extrusion-based three-dimensional(EB-3D)printing technique has been widely used in the complex fabrication of bone tissue-engineered scaffolds,a natural bone-like radial-gradient scaffold by this processing method is of huge challenge and still unmet.Inspired by a typical fractal structure of Koch snowflake,for the first time,a fractal-like porous scaffold with a controllable hierarchical gradient in the radial direction is presented via fractal design and then implemented by EB-3D printing.This radial-gradient structure successfully mimics the radially gradual decrease in porosity of natural bone from cancellous bone to cortical bone.First,we create a design-to-fabrication workflow with embedding the graded data on basis of fractal design into digital processing to instruct the extrusion process of fractal-like scaffolds.Further,by a combination of suitable extruded inks,a series of bone-mimicking scaffolds with a 3-iteration fractal-like structure are fabricated to demonstrate their superiority,including radial porosity,mechanical property,and permeability.This study showcases a robust strategy to overcome the limitations of conventional EB-3D printers for the design and fabrication of functionally graded scaffolds,showing great potential in bone tissue engineering.展开更多
A combination of Fresnel law and machine learning method is proposed to identify the layer counts of 2D materials.Three indexes,which are optical contrast,red-green-blue,total color difference,are presented to illustr...A combination of Fresnel law and machine learning method is proposed to identify the layer counts of 2D materials.Three indexes,which are optical contrast,red-green-blue,total color difference,are presented to illustrate and simulate the visibility of 2D materials on Si/SiO_(2) substrate,and the machine learning algorithms,which are k-mean clustering and k-nearest neighbors,are employed to obtain thickness database of 2D material and test the optical images of 2D materials via red-green-blue index.The results show that this method can provide fast,accurate and large-area property of 2D material.With the combination of artificial intelligence and nanoscience,this machine learning assisted method eases the workload and promotes fundamental research of 2D materials.展开更多
基金support for this work from the National Key R&D Program of China(No.2018YFA0703100)the National Natural Sci-ence Foundation of China(Nos.32122046,82072082,and 32000959)+2 种基金the Youth Innovation Promotion Association of CAS(No.2019350)the Guangdong Natural Science Foundation(No.2019A1515111197)the Shenzhen Fundamental Research Foun-dation(Nos.JCYJ20190812162809131,JCYJ20200109114006014,JCYJ20210324113001005,and JCYJ20210324115814040).
文摘The significance of bioink suitability for the extrusion bioprinting of tissue-like constructs cannot be overemphasized.Gelatin,derived from the hydrolysis of collagen,not only can mimic the extracellular matrix to immensely support cell function,but also is suitable for extrusion under certain conditions.Thus,gelatin has been recognized as a promising bioink for extrusion bioprinting.However,the development of a gelatin-based bioink with satisfactory printability and bioactivity to fabricate complex tissue-like constructs with the desired physicochemical properties and biofunctions for a specific biomedical application is still in its infancy.Therefore,in this review,we aim to comprehensively summarize the state-of-the-art methods of gelatin-based bioink application for extrusion bioprinting.Wefirstly outline the properties and requirements of gelatin-based bioinks for extrusion bioprinting,highlighting the strategies to overcome their main limitations in terms of printability,structural stability and cell viability.Then,the challenges and prospects are further discussed regarding the development of ideal gelatin-based bioinks for extrusion bioprinting to create complex tissue-like constructs with preferable physicochemical properties and biofunctions.
文摘BACKGROUND Postoperative atrial fibrillation(POAF)is a common yet understudied clinical issue after coronary artery bypass graft(CABG)leading to higher mortality rates and stroke.This systematic review and metaanalysis evaluated the rates of adverse outcomes between patients with and without POAF in patients treated with CABG or combined procedures.METHODS The search period was from the beginning of PubMed and Embase to May 18th,2020 with no language restrictions.The inclusion criteria were:(1)studies comparing new onset atrial fibrillation before or after revascularization vs.no new onset AF before or after revascularization.The outcomes assessed included allcause mortality,cardiac death,cerebral vascular accident(CVA),myocardial infarction(MI),repeated revascularization,major adverse cardiac event(MACE),and major adverse cardiac and cerebrovascular events(MACCEs).RESULTS Of the 7,279 entries screened,11 studies comprising of 57,384 patients were included.Compared to nonPOAF,POAF was significantly associated with higher risk of allcause mortality(Risk Ratio(RR)=1.58;95%Confidence Interval(CI):1.42−1.76,P<0.00001)with accompanying high level of heterogeneity(I^(2)=62%).Conclusions Patients with POAF after CABG or combined procedures are at an increased risk of allcause mortality or CVAs.Therefore,POAF after such procedures should be closely monitored and treated judiciously to minimize risk of further complications.While there are studies on POAF versus no POAF on outcomes,the heterogeneity suggests that further studies are needed.
基金the support for this work from the National Natural Science Foundation of China [Grant Nos.32122046,32000959,82030067,and 82161160342]the National Key R&D Program [Grant No.2018YFA0703100]+3 种基金the Youth Innovation Promotion Association of CAS [Grant No.2019350]the Guangdong Natural Science Foundation [Grant No.2020A1515111190]the Shenzhen Fundamental Research Foundation [Grant Nos.JCYJ20190812162809131,JCYJ20200109114006014,JCYJ20210324113001005,JCYJ20210324115814040,and JSGGKQTD20210831174330015]the Shenzhen Fund for Guangdong Provincial High-level Clinical Key Specialties [Grant No.SZGSP001]。
文摘Numerous small-molecule amines(SMAs)play critical roles in maintaining bone homeostasis and promoting bone regeneration regardless of whether they are applied as drugs or biomaterials.On the one hand,SMAs promote bone formation or inhibit bone resorption through the regulation of key molecular signaling pathways in osteoblasts/osteoclasts;on the other hand,owing to their alkaline properties as well as their antioxidant and anti-inflammatory features,most SMAs create a favorable microenvironment for bone homeostasis.However,due to a lack of information on their structure/bioactivity and underlying mechanisms of action,certain SMAs cannot be developed into drugs or biomaterials for bone disease treatment.In this review,we thoroughly summarize the current understanding of SMA effects on bone homeostasis,including descriptions of their classifications,biochemical features,recent research advances in bone biology and related regulatory mechanisms in bone regeneration.In addition,we discuss the challenges and prospects of SMA translational research.
基金supported by grants from the National Natural Science Foundation of China(Nos.32000931,81672632,81972312,and 82103184)the Natural Science Foundation of Hunan Province for outstanding Young Scholars(No.2021JJ20083)+2 种基金Natural Science Foundation of Hunan Province of China(Nos.2021JJ30912,2021JJ40720 and 2021JJ30950)the science and technology innovation Program of Hunan Province(No.2022RC1165)the Open Sharing Fund for the Largescale Instruments and Equipment of Central South University,Changsha,China.
文摘Tumor vascular dysfunction and immune suppression predict poor outcomes of tumor therapy.Combination of photothermal therapy(PTT)and vessel normalization with tumor immunotherapy is promising to augment antitumor benefit.Herein,we develop a potential immunostimulatory nanomodulator for treatment of triple-negative breast cancer(TNBC)treatment via synergism of PTT,vessel normalization,and priming of tumoral suppressive immune microenvironment by blocking transforming growth factor-β(TGF-β)pathway.The nanomodulator,namely Vac@Apt@BPs,is developed by conjugation of TGF-βinhibitor Vactosertib(Vac)and nucleolin-recognizing aptamer(Apt)on the surface of black phosphorus nanoparticles(BPs).Vac@Apt@BPs show good accumulation in TNBC via aptamer-induced active targeting of TNBC.Via the blockade of TGF-βsignaling,Vac@Apt@BPs effectively inhibit the formation of tumor neovascular,and normalize the vessels to recover vascular integrity and alleviate the hypoxia stress.Together with the tumor eradication and immunogenic cell death via PTT,robust immune response was boosted by promoted maturation of dendritic cells,suppression of regulatory T cells,and stimulation of effective T cells.This synergistic therapeutic strategy potentially suppresses the growth of TNBC in mice.
基金This work was supported by the National Natural Science Foundation of China(Project 31570957)the National Key Research and Development Program of China(2017YFB0702504)+1 种基金International Cooperation Project by Science and Technology Department of Sichuan Province(2019YFH0103)Applied Basic Research Project funded by Sichuan Provincial Science and Technology Department(2017JY0296).
文摘Integration of two or more biomolecules with synergetic and complementary effects on a material surface can help to obtain multi-functions for various biomedical applications.However,the amounts of biomolecules integrated and their physiological functions are compromised due to the limited surface anchoring sites.Herein,we propose a novel concept of film engineering strategy“from surface to bulk synergetic modification”.This new concept is realized by employing the surface amine groups of plasma polymerized allylamine(PPAm)film for grafting a molecule e.g.,thrombin inhibitor,bivalirudin(BVLD),meanwhile its bulk amine groups is used as a universal depot for storing and releasing therapeutic nitric oxide(NO)gas as supplement to the functions of BVLD.It is demonstrated that such a“from surface to bulk synergetic modification”film engineering can impart the modified-substrates with anti-platelet and anti-coagulant dual functions,giving rise to a highly endotheliummimetic thromboresistant property.We believe that our research provides a very promising strategy to deliver multifunctional surface versatilely that require NO release in combination with other properties,which will find broad biomedical applications in blood-contacting devices,and et al.Moreover,it also provides a brand-new film engineering strategy for tailoring surface multi-functionalities of a wide range of materials.
基金The authors thank Dr. Zhonghua Hao for technique help. The authors acknowledge financial support from the National Basic Research Program of China (No. 2011CB922201), the National Natural Science Foundation of China (Nos. 51372175 and 11374236), and the Fundamental Research Funds for the Central Universities (No. 2014202020203).
基金The authors gratefully acknowledge the support for this work from the National Key research and Development Program(Grant No.2018YFA0703100)the National Natural Science Foundation of China(Grant Nos.82072082,31900959)+2 种基金the Youth Innovation Promotion Association of CAS(Grant No.2019350)the Guangdong Natural Science Foundation(Grant No.2019A1515011277)the Shenzhen Fundamental Research Foundation(Grant No.JCYJ20180507182237428).
文摘Low patency ratio of small-diameter vascular grafts remains a major challenge due to the occurrence of thrombosis formation and intimal hyperplasia after transplantation.Although developing the functional coating with release of bioactive molecules on the surface of small-diameter vascular grafts are reported as an effective strategy to improve their patency ratios,it is still difficult for current functional coatings cooperating with spatiotemporal control of bioactive molecules release to mimic the sequential requirements for antithrombogenicity and endothelialization.Herein,on basis of 3D-printed polyelectrolyte-based vascular grafts,a biologically inspired release system with sequential release in spatiotemporal coordination of dual molecules through an electrostatic self-assembly was first described.A series of tubes with tunable diameters were initially fabricated by a coaxial extrusion printing method with customized nozzles,in which a polyelectrolyte ink containing of ε-polylysine and sodium alginate was used.Further,dual bioactive molecules,heparin with negative charges and Tyr-Ile-Gly-Ser-Arg(YIGSR)peptide with positive charges were layer-by-layer assembled onto the surface of these 3D-printed tubes.Due to the electrostatic interaction,the sequential release of heparin and YIGSR was demonstrated and could construct a dynamic microenvironment that was thus conducive to the antithrombogenicity and endothelialization.This study opens a new avenue to fabricate a small-diameter vascular graft with a biologically inspired release system based on electrostatic interaction,revealing a huge potential for development of small-diameter artificial vascular grafts with good patency.
基金supported by the National Basic Research Program(2014CB932000)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB14000000)the National Natural Science Foundation of China(21425731,21637004)
文摘Gold nanoparticles(AuNPs) have been attractive for nanomedicine because of their pronounced optical properties.Here,we customerized the methods to synthesize two types of gold nanostars,Au nanostars-1 and Au nanostars-2,which have different spire lengths and optical properties,and also spherical AuNPs.Compared to nanospheres,gold nanostars were less toxic to a variety of cells,including macrophages.Au nanostars-1 and Au nanostars-2 also manifested a similar pattern of tissue distribution upon in vivo administration in mice to that of nanospheres,and but reveled less liver retention than nanospheres.Due to their strong absorption in the near-infrared(NIR),Au nanostars-2 induced a strong hyperthermia effect in vitro upon excitation at 808 nm,and elicited a robust photothermal therapy(PTT) efficacy in ablating tumors in a mouse model of orthotopic breast cancer using 4T1 breast cancer cells.Meanwhile,Au nanostars-1 showed a great capability to enhance the Raman signal through surface-enhanced Raman spectroscopy(SERS) in 4T1 cells.Our combined results opened a new avenue to develop Au nanostars for cancer imaging and therapy.
基金supported by the National Natural Science Foundation of China (Nos. 11532004 and 31370946)the National Science and Technology Support Program of China (No. 2012BAI17B03-4)+1 种基金the Development of Strategic Emerging Industries of Shenzhen Project (Nos. JCYJ20140417113430596 and CXZZ201404171134 30716)the Science and Technology Project of Guangdong Province (No. 2015A010105021)
文摘The effect of particle shape on the porosity and compressive strength of porous hydroxyapatite(HA)scaffolds was investigated by sintering the mixture of rod-shaped HA(r-HA) and spherical HA(s-HA)with polyacrylamide used as the sacrificial template. It was found, for the first time, that addition of r-HA into s-HA could exponentially decrease the porosity of sintered HA scaffolds and enhance their compressive strength with the increase of r-HA content. The mechanism, according to the results from scanning electron microscopy and X-ray diffraction, lies in the restriction of s-HA to the grain formation and growth of r-HA during sintering and results in the fusion of r-HA with s-HA. These findings suggest that mixture of r-HA and s-HA might provide a new and facile way to improve the compressive strength of porous HA scaffolds.
基金We acknowledge National Key Research and Development Program of China(2016YFA0201001)National Natural Science Foundation of China(11627801,11232007,11472130,11472236,and 51702351)+3 种基金Shenzhen Science and Technology Innovation Committee(KQJSCX20170331162214306,JCYJ20170413152832151,JCYJ20170818160815002)US National Science Foundation(CBET-1435968)the Leading Talents Program of Guangdong Province(2016LJ06C372)Shenzhen Programs for Science and Technology Development(JSGG20160229204218661).
文摘Elasticity is a fundamental mechanical property of two-dimensional(2D)materials,and is critical for their application as well as for strain engineering.However,accurate measurement of the elastic modulus of 2D materials remains a challenge,and the conventional suspension method suffers from a number of drawbacks.In this work,we demonstrate a method to map the in-plane Young’s modulus of mono-and bi-layer MoS_(2) on a substrate with high spatial resolution.Bimodal atomic force microscopy is used to accurately map the effective spring constant between the microscope tip and sample,and a finite element method is developed to quantitatively account for the effect of substrate stiffness on deformation.Using these methods,the in-plane Young’s modulus of monolayer MoS_(2) can be decoupled from the substrate and determined as 265±13 GPa,broadly consistent with previous reports though with substantially smaller uncertainty.It is also found that the elasticity of mono-and bi-layer MoS_(2) cannot be differentiated,which is confirmed by the first principles calculations.This method provides a convenient,robust and accurate means to map the in-plane Young’s modulus of 2D materials on a substrate.
文摘Cancer has become the leading cause of death.The progress in diagnosis and treatment is still limited.Over the past three decades,emergence and rapid development of nanotechnology have brought new hopes for cancer therapy.A repertoire of nanomaterials with controllable size-,shape-,and composition-dependent physiochemical proper
文摘Nanomaterials with strong near-infrared absorption and high photothermal conversion ability have shown great application potential in many areas including cancer treatment.However,the current photothermal conversion nanomaterials generally have poor biodegradability and would stay in
基金the financial support from the National Natural Science Foundation of China(No.32122046,12272032,82072082,32101102)the National Key R&D Program of China(No.2020YFC0122204,2018YFA0703100)+2 种基金the Youth Innovation Promotion Association of CAS[No.2019350]the Shenzhen Fundamental Research Foundation[No.JCYJ20210324115814040]the 111 Project(No.B13003).
文摘In the field of regenerative medicine,the importance of 3D bioprinting is self-evident and nonnegligible.However,3D bioprinting technology also requires bioink with excellent performance as support material to fabricate a multi-functional bioinspired scaffold.Collagen-based bioink is regarded as an ideal 3D bioprinting ink for its excellent biocompatibility,controllable printability and cell loading property.It is an important breakthrough in regenerative medicine with the progress of collagen-based bioink,which fabricates bioinspired scaffolds with different functions and is applied in different repair scenarios.This review summarizes the different applications of collagen-based bioink and classifies them as soft tissue and hard tissue according to the target region.The applications of target region in soft tissues include skin,cartilage,heart and blood vessels,while in hard tissues include femur,skull,teeth and spine.When the collagen-based bioink is applied in repairing soft tissue,the requirements of function are higher,while the mechanical properties must be further improved in repairing hard tissue.We further summarize the characteristics of collagen-based bioink and point out the most important properties that should be considered in different repair scenarios,which can provide reference for the preparation of bioinks with different functions.Finally,we point out the main challenges faced by collagen-based bioink and prospect the future research directions.
基金supported by the Major State Basic Research Development Program of China(973 Program)(No.2015CB755500)National Natural Science Foundation of China(Nos.31571013,21375141,81501580,81401521,81301272 and 81571745)+3 种基金Shenzhen Science and Technology Program(Nos.KQCX20140521115045447,JCYJ20150403091443298,JCYJ20130402092657771,JCYJ20150401145529015 and JCYJ20160229200902680)Instrument Developing Project of the CAS(No.YZ201439)Key International S&T Cooperation Project(No.2015DFH50230)Guangdong Natural Science Foundation of Research Team(2016A030312006)
文摘As one of near-infrared(NIR) fluorescent(FL) nanoprobes, gold nanoclusters(Au NCs) are delicated to passive-targeting tumors for NIR FL imaging, but which easily cleared by the kidneys for the small size(<1.5 nm). Herein, the well-defined gold clusters nanoassembly(Au CNA) was synthesized by the selfassembly of Au NCs based on protein cross-linking approach. The as-prepared Au CNA demonstrated highly effective cellular uptake and precise tumor targeting compared to that of Au NCs. Moreover, with the irradiation of 660 nm laser, Au CNA generated largely reactive oxygen species(ROS) for photodynamic therapy(PDT). In vitro and [39_TD$IF]in vivo PDT revealed that Au CNA exhibited largely cell death and significantly tumor removal at a low power density of 0.2 W/cm^2. It could be speculated that the laser-excited Au CNA produced photon energy, which further obtained electron from oxygen to generate radical species.Therefore, Au CNA as a photosensitizer could realize NIR FL imaging and NIR laser induced PDT.
基金supported by the National Natural Science Foundation of China(31000323,31070672,81250044)the Natural Science Foundation of Jiangsu Province(BK20131272)+1 种基金the Specialized Research Fund for the Doctoral Program of Higher Education of China(20100091120023)the Fundamental Research Funds for the Central Universities(1095020823)
文摘Spectrin is a large,cytoskeletal,and heterodimeric protein composed of modular structure of and subunits,it typically contains 106 contiguous amino acid sequence motifs called"spectrin repeats".Spectrin is crucial for maintaining the stability and structure of the cell membrane and the shape of a cell.Moreover,it contributes to diverse cell functions such as cell adhesion,cell spreading,and the cell cycle.Mutations of spectrin lead to various human diseases such as hereditary hemolytic anemia,type 5 spinocerebellar ataxia,cancer,as well as others.This review focuses on recent advances in determining the structure and function of spectrin as well as its role in disease.
基金This research was supported by Shenzhen Key Laboratory of Inflammatory and Immunology Diseases(No.ZDSYS 20200811143756018)China Postdoctoral Science Foundation(No.2021M693290)+1 种基金the Key Program for Basic Research of Shenzhen Science and Technology Innovation Commission(No.JCYJ20200109140203849)Guangdong Basic and Applied Basic Research Foundation(No.2021A1515111000).
文摘Liver disease has long been a heavy health and economic burden worldwide.Once the disease is out of control and progresses to end-stage or acute organ failure,orthotopic liver transplantation(OLT)is the only therapeutic alternative,and it requires appropriate donors and aggressive administration of immunosuppressive drugs.Therefore,hepatocyte transplantation(HT)and bioartificial livers(BALs)have been proposed as effective treatments for acute liver failure(ALF)in clinics.Although human primary hepatocytes(PHs)are an ideal cell source to support these methods,the large demand and superior viability of PH is needed,which restrains its wide usage.Thus,a finding alternative to meet the quantity and quality of hepatocytes is urgent.In this context,human pluripotent stem cells(PSC),which have unlimited proliferative and differential potential,derived hepatocytes are a promising renewable cell source.Recent studies of the differentiation of PSC into hepatocytes has provided evidence that supports their clinical application.In this review,we discuss the recent status and future directions of the potential use of PSC-derived hepatocytes in treating ALF.We also discuss opportunities and challenges of how to promote such strategies in the common applications in clinical treatments.
基金supported by grants from the Emergency Prevention and Control of COVID-19 Project of Henan Province(grant no.201100310900)the 2020 Science and Technology Project of Henan Province(grant no.202102310039)+1 种基金the National Natural Science Foundation of China(grant nos.91942314,U1804281,81602024)the State’s Key Project of Research and Development Plan(grant nos.2018YFC1313400,2016YFC1303500)。
文摘The recent novel coronavirus disease(COVID-19)outbreak,caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is seeing a rapid increase in infected patients worldwide.The host immune response to SARS-CoV-2 appears to play a critical role in disease pathogenesis and clinical manifestations.SARS-CoV-2 not only activates antiviral immune responses,but can also cause uncontrolled inflammatory responses characterized by marked pro-inflammatory cytokine release in patients with severe COVID-19,leading to lymphopenia,lymphocyte dysfunction,and granulocyte and monocyte abnormalities.These SARS-CoV-2-induced immune abnormalities may lead to infections by microorganisms,septic shock,and severe multiple organ dysfunction.Therefore,mechanisms underlying immune abnormalities in patients with COVID-19 must be elucidated to guide clinical management of the disease.Moreover,rational management of the immune responses to SARSCoV-2,which includes enhancing anti-viral immunity while inhibiting systemic inflammation,may be key to successful treatment.In this review,we discuss the immunopathology of COVID-19,its potential mechanisms,and clinical implications to aid the development of new therapeutic strategies against COVID-19.
基金This work was supported by grants from the National Natural Science Foundation of China(No.51272274,81672227,51372170)Shenzhen Peacock Program(No.110811003586331)Shenzhen Science and Technology Research funding(No.CXZZ 20150401152251209,JSGG20151030140325149,JSGG20150331154931068,CXZZ20140417113430716)and partially from Hong Kong General Research Fund.
文摘In osteoporosis scenario, tissue response to implants is greatly impaired by the deteriorated boneregeneration microenvironment. In the present study, a Mg-containing akermanite (Ak) ceramic wasemployed for the treatment of osteoporotic bone defect, based on the hypothesis that both beneficialions (e.g. Mg^2+ ect.) released by the implants and the weak alkaline microenvironment pH (μe-pH) itcreated may play distinct roles in recovering the abnormal bone regeneration by stimulating osteoblasticanabolic effects. The performance of Ak, b-tricalcium phosphate (β-TCP) and Hardystone (Har) in healinga 3 mm bone defect on the ovariectomized (OVX) osteoporotic rat model was evaluated. Our resultsindicated that, there's more new bone formed in Ak group than in β-TCP or Har group at week 9. Theinitial me-pHs of Ak were significantly higher than that of the β-TCP and Blank group, and this weakalkaline condition was maintained till at least 9 weeks post-surgery. Increased osteoblastic activity whichwas indicated by higher osteoid secretion was observed in Ak group at week 4 to week 9. An intermediatelayer which was rich in phosphorus minerals and bound directly to the new forming bone wasdeveloped on the surface of Ak. In a summary, our study demonstrates that Ak exhibits a superior boneregenerative performance under osteoporosis condition, and might be a promising candidate for thetreatment of osteoporotic bone defect and fracture.
基金supported by the National Key Research and Development Program of China(grant no.2018YFA0703100)the National Natural Science Foundation of China(grant nos.32122046,82072082,and 32000959)+2 种基金the Youth Innovation Promotion Association of CAS(grant no.2019350)the Guangdong Natural Science Foundation(grant no.2019A1515011277)the Shenzhen Fundamental Research Foundation(grant nos.JCYJ20180507182237428 and JCYJ20190812162809131).
文摘Although extrusion-based three-dimensional(EB-3D)printing technique has been widely used in the complex fabrication of bone tissue-engineered scaffolds,a natural bone-like radial-gradient scaffold by this processing method is of huge challenge and still unmet.Inspired by a typical fractal structure of Koch snowflake,for the first time,a fractal-like porous scaffold with a controllable hierarchical gradient in the radial direction is presented via fractal design and then implemented by EB-3D printing.This radial-gradient structure successfully mimics the radially gradual decrease in porosity of natural bone from cancellous bone to cortical bone.First,we create a design-to-fabrication workflow with embedding the graded data on basis of fractal design into digital processing to instruct the extrusion process of fractal-like scaffolds.Further,by a combination of suitable extruded inks,a series of bone-mimicking scaffolds with a 3-iteration fractal-like structure are fabricated to demonstrate their superiority,including radial porosity,mechanical property,and permeability.This study showcases a robust strategy to overcome the limitations of conventional EB-3D printers for the design and fabrication of functionally graded scaffolds,showing great potential in bone tissue engineering.
基金National Key Research and Development Program of China(2016YFA0201001)National Natural Science Foundation of China(11627801,11472130,11872203,and 11572276)+3 种基金Shenzhen Science and Technology Innovation Committee(JCYJ20170818160815002)Shenzhen Science and Technology Research Funding(JCYJ20160608141439330)Natural Science Foundation of Xinjiang(2017D01C055)Wuhan University of Technology(2018-KF-14).
文摘A combination of Fresnel law and machine learning method is proposed to identify the layer counts of 2D materials.Three indexes,which are optical contrast,red-green-blue,total color difference,are presented to illustrate and simulate the visibility of 2D materials on Si/SiO_(2) substrate,and the machine learning algorithms,which are k-mean clustering and k-nearest neighbors,are employed to obtain thickness database of 2D material and test the optical images of 2D materials via red-green-blue index.The results show that this method can provide fast,accurate and large-area property of 2D material.With the combination of artificial intelligence and nanoscience,this machine learning assisted method eases the workload and promotes fundamental research of 2D materials.