The antioxidant and antibacterial activities of the phytochemical constituents of the pericarp, mesocarp and segment membrane crude ethanolic extracts of Pummelo (Citrus maxima (Burm.)) fruit were were tested against ...The antioxidant and antibacterial activities of the phytochemical constituents of the pericarp, mesocarp and segment membrane crude ethanolic extracts of Pummelo (Citrus maxima (Burm.)) fruit were were tested against Escherichia coli and Salmonella typhimurium. Preliminary phytochemical test revealed the presence of phenols, tannins, saponins expressed as catechine quivalent (CE)/100ml and flavonoid expressed as gallic acid equivalent (GAE)/100ml. The order of which was as follows pericarp > segment membrane > mesocarp. The strongest antioxidant activity was obtained by the pericarp extract (29.64 expressed as % lipid peroxidation). The differences in the measured amount of phytochemicals and antioxidant activity among the three sample extracts were found to be significant. In terms of antimicrobial activity, the pericarp, mesocarp and segment membrane extracts generated zone of inhibitions measuring 17.10, 18.00 and 17.03 mm for S. typhimurium, respectively at 100% concentration. E. coli was noted to be inactive in all three sample extracts at 100% concentration. The capacity of E. coli to counteract the inhibitory effect of the phytochemicals contained in the pummelo extracts may be attributed to its rough corrugated cell wall and thick periplasmic space as opposed to the smooth curved and barely seen periplasmic space of S. typhimurium. However, no significant correlation was detected among the phytochemical content, antioxidant and in vitro antimicrobial activities of the sample extracts against S. typhimurium.展开更多
The study was designed to produce a nutrient-dense polvoron bar from the optimized mixture of Saba-peel, monggo, and malunggay using simplex lattice design (RSM-SLD). Polvoron bar was also evaluated analytically inclu...The study was designed to produce a nutrient-dense polvoron bar from the optimized mixture of Saba-peel, monggo, and malunggay using simplex lattice design (RSM-SLD). Polvoron bar was also evaluated analytically including proximate, iron, total dietary fiber (TDF), and vitamin A content. Shelf stability, consumer acceptability, and microbial assessment of the sample were determined. Results showed that the optimal combination of ingredients was 37%, 52%, and 11% for saba peel flour, monggo flour, and malunggay powder, respectively. Proximate composition revealed that a fresh sample can provide 66% of carbohydrates, 12.17% protein, and 17% of fats. Every 100 g has 1.43 mg Fe content, 1.78 g TDF, and 91.25 mg vitamin A. The optimized product can last up to 111, 98, and 54 days at 30°C, 40°C, and 50°C, respectively, while total aerobic bacteria, yeast and molds, and coliform count are within the acceptable limits of the FDA standards.展开更多
The 2–5%γδT cells in healthy human peripheral blood recognize nonpeptide antigens,in contrast toαβT cells.1 DistinctγδT-cell malignancies have been identified,with hepatosplenic T-cell lymphoma(γδ-HSTL)being ...The 2–5%γδT cells in healthy human peripheral blood recognize nonpeptide antigens,in contrast toαβT cells.1 DistinctγδT-cell malignancies have been identified,with hepatosplenic T-cell lymphoma(γδ-HSTL)being a rare but aggressive(median survival 13 months)subset.2γδ-HSTL usually presents with hepatosplenomegaly without lymphadenopathy,anemia,and thrombocytopenia.It is histologically characterized by the involvement of the hepatic sinusoids,splenic red pulp and bone marrow interstitium,with or without leukemic presentation in peripheral blood.3 A standard treatment regimen is not well established,but therapy mostly includes various chemotherapeutic agents.Notably,interferon-α(IFN-α)has been proven to be effective in some patients withγδ-HSTL,but other patients require allogeneic or autologous stem cell transplantation.展开更多
基金The authors acknowledge the Department of Science and Technology,Philippines,for the financial support through a PhD scholarship for A.S.A.Barrion.
文摘The antioxidant and antibacterial activities of the phytochemical constituents of the pericarp, mesocarp and segment membrane crude ethanolic extracts of Pummelo (Citrus maxima (Burm.)) fruit were were tested against Escherichia coli and Salmonella typhimurium. Preliminary phytochemical test revealed the presence of phenols, tannins, saponins expressed as catechine quivalent (CE)/100ml and flavonoid expressed as gallic acid equivalent (GAE)/100ml. The order of which was as follows pericarp > segment membrane > mesocarp. The strongest antioxidant activity was obtained by the pericarp extract (29.64 expressed as % lipid peroxidation). The differences in the measured amount of phytochemicals and antioxidant activity among the three sample extracts were found to be significant. In terms of antimicrobial activity, the pericarp, mesocarp and segment membrane extracts generated zone of inhibitions measuring 17.10, 18.00 and 17.03 mm for S. typhimurium, respectively at 100% concentration. E. coli was noted to be inactive in all three sample extracts at 100% concentration. The capacity of E. coli to counteract the inhibitory effect of the phytochemicals contained in the pummelo extracts may be attributed to its rough corrugated cell wall and thick periplasmic space as opposed to the smooth curved and barely seen periplasmic space of S. typhimurium. However, no significant correlation was detected among the phytochemical content, antioxidant and in vitro antimicrobial activities of the sample extracts against S. typhimurium.
文摘The study was designed to produce a nutrient-dense polvoron bar from the optimized mixture of Saba-peel, monggo, and malunggay using simplex lattice design (RSM-SLD). Polvoron bar was also evaluated analytically including proximate, iron, total dietary fiber (TDF), and vitamin A content. Shelf stability, consumer acceptability, and microbial assessment of the sample were determined. Results showed that the optimal combination of ingredients was 37%, 52%, and 11% for saba peel flour, monggo flour, and malunggay powder, respectively. Proximate composition revealed that a fresh sample can provide 66% of carbohydrates, 12.17% protein, and 17% of fats. Every 100 g has 1.43 mg Fe content, 1.78 g TDF, and 91.25 mg vitamin A. The optimized product can last up to 111, 98, and 54 days at 30°C, 40°C, and 50°C, respectively, while total aerobic bacteria, yeast and molds, and coliform count are within the acceptable limits of the FDA standards.
基金supported by the Deutsche Forschungsgemeinschaft(DFG)through the Cluster of Excellence“Inflammation-at-Interfaces”EXC306-PN and EXC306-PM(D.K.,O.A.and C.K.)the Werner-und-Klara-Kreitz Foundation(JB).
文摘The 2–5%γδT cells in healthy human peripheral blood recognize nonpeptide antigens,in contrast toαβT cells.1 DistinctγδT-cell malignancies have been identified,with hepatosplenic T-cell lymphoma(γδ-HSTL)being a rare but aggressive(median survival 13 months)subset.2γδ-HSTL usually presents with hepatosplenomegaly without lymphadenopathy,anemia,and thrombocytopenia.It is histologically characterized by the involvement of the hepatic sinusoids,splenic red pulp and bone marrow interstitium,with or without leukemic presentation in peripheral blood.3 A standard treatment regimen is not well established,but therapy mostly includes various chemotherapeutic agents.Notably,interferon-α(IFN-α)has been proven to be effective in some patients withγδ-HSTL,but other patients require allogeneic or autologous stem cell transplantation.
