Background H9N2 avian influenza viruses （AIVs） have repeatedly caused infections in mammals even humans in many countries. The purpose of our study was to evaluate the acute lung injury （ALl） caused by H9N2 viral ...Background H9N2 avian influenza viruses （AIVs） have repeatedly caused infections in mammals even humans in many countries. The purpose of our study was to evaluate the acute lung injury （ALl） caused by H9N2 viral infection in mice. Methods Six- to eight- week-old female SPF C57BL/6 mice were infected intranasally with lx104 MIDso of A/HONG KONG/2108/2003 [H9N2 （HK）] virus. Clinical signs, pathological changes, virus titration in tissues of mice, arterial blood gas, and cytokines in bronchoalveolar lavage fluid （BALF） and serum were observed at different time points after AIV infection. Results H9N2-AIV-infected mice exhibited severe respiratory syndrome, with a mortality rate of 50%. Lung histopathological changes in infected mice included diffuse pneumonia, alveolar damage, inflammatory cellular infiltration, interstitial and alveolar edema, and hemorrhage. In addition, HgN2 viral infection resulted in severe progressive hypoxemia, lymphopenia, and a significant increase in interleukin 1, interleukin 6, tumor necrosis factor, and interferon in BALF and serum. Conclusions The results suggest that H9N2 viral infection induces a typical ALl in mice that resembles the common features of ALl. Our data may facilitate the future studies of potential avian H9N2 disease in humans.展开更多
What is already known about this topic?Dengue fever(DF)outbreaks affect hundreds of millions of people worldwide and have increased significantly in Guangdong Province in 2019.What is added by this report?This paper d...What is already known about this topic?Dengue fever(DF)outbreaks affect hundreds of millions of people worldwide and have increased significantly in Guangdong Province in 2019.What is added by this report?This paper described briefly DF outbreaks were attributed to several types of dengue virus(DENV)including DENV-1,DENV-2,and DENV-3 in 2019 in Guangdong,tracked the sources of viruses through phylogenetic analysis and epidemiological investigation,and primarily revealed the epidemiological links among the outbreaks.What are the implications for public health practice?The introduction of DENV from DF endemic areas increased pressure on the prevention and control of DF in Guangdong.Early detection of suspected cases and typing and genotyping of circulating viruses should be prioritized and enhanced to promptly assess the likelihood of local transmission,of introduction,and of subsequent sustained local transmission of the virus to implement optimal prevention and control strategies and measures.展开更多
基金This work was supported by grants trom the National Natural Science Foundation of China （No. 81170054, No. 81301448）, the Natural Science Foundation of Jiangsu Province of China （No. BK2011570）, the National Basic Research Program of China （973 program No. 2010CB945103）.
文摘Background H9N2 avian influenza viruses （AIVs） have repeatedly caused infections in mammals even humans in many countries. The purpose of our study was to evaluate the acute lung injury （ALl） caused by H9N2 viral infection in mice. Methods Six- to eight- week-old female SPF C57BL/6 mice were infected intranasally with lx104 MIDso of A/HONG KONG/2108/2003 [H9N2 （HK）] virus. Clinical signs, pathological changes, virus titration in tissues of mice, arterial blood gas, and cytokines in bronchoalveolar lavage fluid （BALF） and serum were observed at different time points after AIV infection. Results H9N2-AIV-infected mice exhibited severe respiratory syndrome, with a mortality rate of 50%. Lung histopathological changes in infected mice included diffuse pneumonia, alveolar damage, inflammatory cellular infiltration, interstitial and alveolar edema, and hemorrhage. In addition, HgN2 viral infection resulted in severe progressive hypoxemia, lymphopenia, and a significant increase in interleukin 1, interleukin 6, tumor necrosis factor, and interferon in BALF and serum. Conclusions The results suggest that H9N2 viral infection induces a typical ALl in mice that resembles the common features of ALl. Our data may facilitate the future studies of potential avian H9N2 disease in humans.
文摘What is already known about this topic?Dengue fever(DF)outbreaks affect hundreds of millions of people worldwide and have increased significantly in Guangdong Province in 2019.What is added by this report?This paper described briefly DF outbreaks were attributed to several types of dengue virus(DENV)including DENV-1,DENV-2,and DENV-3 in 2019 in Guangdong,tracked the sources of viruses through phylogenetic analysis and epidemiological investigation,and primarily revealed the epidemiological links among the outbreaks.What are the implications for public health practice?The introduction of DENV from DF endemic areas increased pressure on the prevention and control of DF in Guangdong.Early detection of suspected cases and typing and genotyping of circulating viruses should be prioritized and enhanced to promptly assess the likelihood of local transmission,of introduction,and of subsequent sustained local transmission of the virus to implement optimal prevention and control strategies and measures.
