In accordance with the objective to construct a major province in grassland animal husbandry,the following ways were used for enhancing development,improving the scientific and technological content,increasing the sca...In accordance with the objective to construct a major province in grassland animal husbandry,the following ways were used for enhancing development,improving the scientific and technological content,increasing the scale of the industry and market development to set up our own ecological grass industry brand and finally reach sustainable development strategy.This paper discussed the status of Guizhou Province grass industry development from the grass industry resources,grassland and animal husbandry production,prataculture technology problem.展开更多
In this paper,an updated vegetation map of the permafrost zone in the Qinghai-Tibet Plateau(QTP) was delineated.The vegetation map model was extracted from vegetation sampling with remote sensing(RS) datasets by decis...In this paper,an updated vegetation map of the permafrost zone in the Qinghai-Tibet Plateau(QTP) was delineated.The vegetation map model was extracted from vegetation sampling with remote sensing(RS) datasets by decision tree method.The spatial resolution of the map is 1 km×1 km,and in it the alpine swamp meadow is firstly distinguished in the high-altitude areas.The results showed that the total vegetated area in the permafrost zone of the QTP is 1,201,751 km^2.In the vegetated region,50,260 km^2 is the areas of alpine swamp meadow,583,909 km^2 for alpine meadow,332,754 km^2 for alpine steppe,and 234,828 km^2 for alpine desert.This updated vegetation map in permafrost zone of QTP could provide more details about the distribution of alpine vegetation types for studying the vegetation mechanisms in the land surface processes of highaltitude areas.展开更多
To select the suitable plant species controlling Eupatorium adenophorum in hilly area, ecological adaptability, competitiveness and control efficacy of many replacement plants with economic value in Guizhou Province o...To select the suitable plant species controlling Eupatorium adenophorum in hilly area, ecological adaptability, competitiveness and control efficacy of many replacement plants with economic value in Guizhou Province of China were studied. The results showed that the coverage of paspalum wetsfeteini and Dolichos lablab were 100% and 93%, which increased by 3.70 and 3.44 times compared with Lolium perenne, respectively; their relative crowding coefficient with E. adenophorum were 7.09 and 22.78, which increased by 2.43 and 7.80 times compared with L perenroe, respectively. Using excavation method, the control efficacies of replacement plants were 99.3 % and 96.9%, respectively, while the control efficacy of replacement plants using mowing method was lower than 66.4%. The overwintering rate of P. wetsfeteini in the following year was 95% ; its coverage was still 100% and its control efficacy against E. adenophorum remained over 99%. D. lablab was difficult to survive the winter in the north region beyond 26.2°N, so it could only be applied as annual replacement plant. The coverage of Setaria anceps, Cajanus cajan and other test plants were less than 90%, with poor control efficacy against E. adenophorum.展开更多
Carex brunnescens(Pers.)Poir.is considered to be the only clonal herb found to date that can develop and form fixed dunes in Maqu alpine degraded grasslands of northwestern China.However,due to strong dormant characte...Carex brunnescens(Pers.)Poir.is considered to be the only clonal herb found to date that can develop and form fixed dunes in Maqu alpine degraded grasslands of northwestern China.However,due to strong dormant characteristics of C.brunnescens seeds,the sand-fixing effect of the plant is severely limited.This study explores a technique that can rapidly promote the seed germination of C.brunnescens,and also investigates the adaptation and sand-fixing effect by cultivating C.brunnescens seedlings to establish living sand barriers in the sand ridges of moving sand dunes.Results show that the seed germination rate obtained a maximum of 63.7%or 65.1%when seeds were treated with 150 mg/L gibberellic acid(GA3)for 24 h followed by soaking in sulfuric acid(98%H2SO4)for 2.5 min or sodium hydroxide(10%NaOH)for 3.5 h,and then germinated(25°C in daytime and 5°C at nighttime)in darkness for 10 d.After breaking seed dormancy of C.brunnescens,the living sand barrier of C.brunnescens(plant spacing 15−20 cm;sand barrier spacing 10−20 m)was established in the perpendicular direction to the main wind in the middle and lower parts of the sand ridges on both sides of the moving sand dunes.When the sand ridges were leveled by wind erosion,the living sand barrier(plant spacing 15−20 cm;sand barrier spacing 0.5−1.0 m)of C.brunnescens was reestablished on the wind-eroded flat ground.Finally,a stable sand-fixing surface can be formed after connecting the living sand barriers on both sides,thus achieving a good sand-fixing effect.These findings suggest that rapid seed germination technology combined with the sand−fixing method of C.brunnescens can shorten the seed germination period and make the seedling establishment become much easier which may be an effective strategy to restore and reconstruct Maqu degraded grasslands.展开更多
The clinical signs of Tibetan sheep with sulphur- and iron-induced copper deficiency in Maqu County of Gansu Province were defined as thin, decreased appetite, neonatal ataxia, pica, emaciation, and loss of hair color...The clinical signs of Tibetan sheep with sulphur- and iron-induced copper deficiency in Maqu County of Gansu Province were defined as thin, decreased appetite, neonatal ataxia, pica, emaciation, and loss of hair color and anemia. The mineral composition of soil and forage, and samples of liver, blood and hair from Tibetan sheep in affected ranches in this region were compared with those from unaffected areas. The mean concentration of iron in soil and forage in affected pasture was 19 675 ±791 and 1 763 ±197 μg/g, respectively, compared with 8 761 ±871 and 357 ±39 μg/g in unaffected pasture. The mean concentration of sulphur in soil and forage in affected pasture was 1.98% ±0.79% and 0.88% ±0.16%, respectively, compared with 1.16%±0.26% and 0.47%±0.16%in unaffected pasture. The mean copper concentrations in soil and forage from the affected pastures were 15.9±1.8 and 6.7±1.6 μg/g, respectively, compared with 16.7±1.9 and 8.9±2.7 μg/g in unaffected pasture.The mean concentration of Cu in blood, liver, and hair from the affected Tibetan sheep were 0.32±0.12, 51.2±3.1 and 2.16±0.65μg/g, respectively, compared with 0.76±0.21, 191±11 and 3.27±0.96 μg/g for healthy animals. Oral administration of CuClO(OH)3prevented and cured anemia. We conclude that wool eating of Tibetan sheep in this region is probably caused by secondary Cu deficiency, mainly due to the high iron and sulphur contents in soils and forage.展开更多
[Objectives] This study was conducted to explore the interaction between nitrogen-fixing and phosphate-solubilizing strains and the optimal combination of different functional strains,in order to provide a theoretical...[Objectives] This study was conducted to explore the interaction between nitrogen-fixing and phosphate-solubilizing strains and the optimal combination of different functional strains,in order to provide a theoretical basis for the development of PGPR compound fertilizers suitable for local environment.[Methods] In this study,16S rDNA gene sequence analysis was used to identify fast-growing and competitive strains from pasture nodules and rhizosphere soils in Guizhou Province,and three representative Rhizobia and phosphorus-solubilizing bacteria were chosen for the test of bacterial combination when reducing 50% of nitrogen and 30% of phosphorus.The effects of different strain combinations on the plant height,root length,aboveground and underground biomass of Lotus corniculatus L.were investigated,and the total nitrogen and total phosphorus contents of the plants were determined.[Results] The mixed bacterial agents could promote the increase of root biomass,and the effects of A1,A3,B3 and C3 were the most obvious.The fresh weight and dry weight of the roots of L.corniculatus increased by 30.35%-168.45% and 26.43%-180.00%,respectively,and A3,B3,B2 and C3 had the best effects.The total phosphorus content of the plants increased by 12.79%-55.25% compared with the CK2;and most of the bacterial agents with significant growth-promoting effects showed decreased total nitrogen contents,while those with non-significant growth-promoting effects showed significantly-increased total nitrogen contents,which were not as much as the CK1.Comprehensively,the most productive combination was C3,namely R27-2 Rhinohizobium fredii and P33-3 Stenotrophomonas rhizophila.[Conclusions] This study can provide a theoretical basis for the production and promotion of bacterial fertilizers.展开更多
Abstract The metabolites of tall fescue leaves under normal and drought conditions were analyzed with LC-ESI-MS ( liquid chromatography tandem mass spec- trometry). A total of 282 metabolites were stably detected in...Abstract The metabolites of tall fescue leaves under normal and drought conditions were analyzed with LC-ESI-MS ( liquid chromatography tandem mass spec- trometry). A total of 282 metabolites were stably detected in the two groups using OPI.S-DA (orthogonal partial least squares discriminant analysis) method; 148 of which were down-regulated while 134 were up-regulated. After analyzed with MZ-minc software, 10 major metabolites (4 down, 6 up) were identified through searehing public database. Aromatic compounds, 3-p-eoumaroyll, 5-quinolaetone, aromatic free amino acids, styraxin mad methanofuran, were up-regulated ; grease metabolites, methyl 15-hydroperoxy-9Z, 12Z, 16E-octadecatrienoate, 9,12,13-Tri-HOME (10), 3-oxo-dodecanoic acid, and 13 (S)-HpOTrE acid ester, were down-regulated; piperanine (alkaloids) was down-regulated while Methyl 7-epi-12-hydroxyjasmonate glucoside (indicant) was up-regulated. In a word, aromatic compounds and liquid metabolites may play vital roles in drought-resistant regulation of tall fescue.展开更多
The air temperature of Wuli,which is located in seasonal frozen ground zone,is rising by 13 ℃ yearly.This paper discusses the days that each ground layers' temperature lags behind the surface temperature in reach...The air temperature of Wuli,which is located in seasonal frozen ground zone,is rising by 13 ℃ yearly.This paper discusses the days that each ground layers' temperature lags behind the surface temperature in reaching extremum.The results were shown:The time of each ground layers' lagging days was increasing;the lagging day in warm season was longer than that in cold season;the growth rate of lagging days in warm season was 0.5 d/y,while the growth rate of lagging days in cold season was 0.7 d/y.展开更多
Yunnan-Guizhou Plateau is the core area of the rocky desertification in the Chinese southwest Karst regions, and the existing studies are very limited to describe the process of rocky desertification quantitatively. T...Yunnan-Guizhou Plateau is the core area of the rocky desertification in the Chinese southwest Karst regions, and the existing studies are very limited to describe the process of rocky desertification quantitatively. The vegetation resources are applied as the key indicators in quantitative description of the degree of rocky desertification damage, and the previous methods using only remote sensing datasets are not competent to distinguish the detailed information of vegetation type, coverage and patch fragmentation on a large scale. Previous research shows that the technology of unmanned aerial vehicle and the estimation software of fraction vegetation coverages and patches could be accurately and rapidly to exploit vegetation resource information. In this project, current distribution of vegetation resources in Yunnan-Guizhou Plateau was clarified to reveal the influence mechanism of the rocky desertification. The control factors of rocky desertification distribution and the driving reasons of its dynamic changes were explained by the data of terrain, climate, population, economics and policy. Therefore, it could get the current distribution of vegetation resources in Yunnan-Guizhou Plateau, especially the grassland resources could be updated, which could not provide research foundations and scientific implications for resolving the rocky desertification, but also could provide valuable background information for the programs of restoring ecological environment and increasing local people's income in Yunnan-Guizhou Plateau.展开更多
Forages generally suffer more severe nutrition stress than other crops, but the corresponding theoretical studies still lag behind. In this study, metabo- lites in tall fescue leaves under normal and low nitrogen cond...Forages generally suffer more severe nutrition stress than other crops, but the corresponding theoretical studies still lag behind. In this study, metabo- lites in tall fescue leaves under normal and low nitrogen conditions were analyzed with LC-ESI-MS ( liquid chromatography-electrospray ionization-mass spectrome- try). A total of 1 424 and 1 251 metabolites were detected from two groups of samples respectively using OPLS-DA (orthogonal partial least squares discriminant analysis). The content of 13 major metabolites changed under low nitrogen stress, including auxin (indale acetic acid, IAA). Quantitative real-time PCR (qRT- PCR) showed that eight genes related to IAA signaling pathway were up-regulated after nitrogen stress. Enzyme-linked immuno sorbent assay (ELISA) revealed that the IAA concentration was elevated in tall fescue leaves after nitrogen stress. Our research provides valuable information for studying the response mechanisms of tall feseue to low nitrogen. The results suggest that application of IAA could be used to alleviate the harmful effects of nitrogen deficiency.展开更多
In this paper, domestic and foreign advances in breeding situation and conventional breeding methods of Festuca arundinacea Schreb. and the applica- tion of modem biological techniques ( somaclonal variation, somatic...In this paper, domestic and foreign advances in breeding situation and conventional breeding methods of Festuca arundinacea Schreb. and the applica- tion of modem biological techniques ( somaclonal variation, somatic cell hybridization, genetic map construction, genetic diversity, gene cloning and expression, ge- netic engineering) in breeding of new varieties of F. arundinacea were summarized, which proposed new ideas for the development of F. arundinacea breeding and provided reference for breeding of new varieties of F. arundinacea.展开更多
The cDNA of PHYC gene of tall fescue ( Festuca arundinacea ) was cloned by RT-PCR,named FaPHYC.The sequence was analyzed by bioinformatic software,and the results showed that the full-length cDNA(3816 bp) was obtained...The cDNA of PHYC gene of tall fescue ( Festuca arundinacea ) was cloned by RT-PCR,named FaPHYC.The sequence was analyzed by bioinformatic software,and the results showed that the full-length cDNA(3816 bp) was obtained with an open reading frame predicated to be 3 411 bp,which encoded 1 136 amino acids.By BLASTP analysis,the N-terminal of FaPHYC was composed of GAF and pPhytochrome domains,and its C-terminal included two repeated PAS domains,one histidine kinase A domain and one histidine kinase-like ATPase domain.The phylogenetic analysis showed that FaPHYC was closely related to PHYCs from other Gramineae plants.The qRT-PCR results showed that FaPHYC expression was regulated by drought stress,heat stress,salt stress and nitrogen stress.The real-time quantitative PCR assay showed the expression of FaPHYC in tall fescue seedlings was continuously up-regulated during the light period and down-regulated in the dark.The results suggest that FaPHYC is photoperiod sensitive and plays a functional role in flowering regulation by photoperiod in tall fescue.The FaPHYC gene was cloned and reported from tall fescue for the first time,which lays a foundation for the investigation of the stress mechanism of FaPHYC in tall fescue plant.展开更多
[ Objective ] This study aimed to construct RNAi expression vector targeting vemalizational gene FaCONSTANS (GenBank accession number: GU214996) in tall rescue. [ Method] A 145 bp long Arabidopsis actin gene intron...[ Objective ] This study aimed to construct RNAi expression vector targeting vemalizational gene FaCONSTANS (GenBank accession number: GU214996) in tall rescue. [ Method] A 145 bp long Arabidopsis actin gene intron was inserted into the expression vector to construct an intermediate vector pBI121-M-INT. Two pairs of specific primers with restriction sites were designed to amplify a 351 bp long cDNA conserved sequence fragment of vemalizational gene FaCONSTANS for RT-PCR. After restriction enzyme digestion, the amplified fragment was inserted forwardly and reversely at two sides of the intron of intermediate vector to construct an RNAi expression vector with hairpin structure. [ Result ] Double digestion (HindIII + BamHI) showed that the intron was successfully insert- ed into the vector pBI121. PCR amplification and double digestion indicated that target fragment FaCONSTANS was successfully inserted forwardly and reversely in- to the intermediate vector. [ Conclusion] This study laid foundation for breeding novel flowering-inhibited tall rescue cultivars.展开更多
Many studies showed that permafrost has profound influence on alpine ecosystem. However, former researches were mainly focused on typical points by temporal scales. There were few studies about the correlation between...Many studies showed that permafrost has profound influence on alpine ecosystem. However, former researches were mainly focused on typical points by temporal scales. There were few studies about the correlation between vegetation characteristics and different altitudes covering a large region in spatial pattern, especially in transitional permafrost(TP). There were continuous permafrost(CP) discontinuous permafrost(DCP) and seasonal frozen ground(SFG) in this study region. The types of permafrost changed from SFG to DCP, and finally become CP as the altitudes of Xidatan increase. In this paper, 112 845 points interpreted by HJ1-B(environment and disaster monitoring and prediction small satellite constellation), vegetation investigation points, thawing layer thickness research sites, ground temperature and water content observation plots were used to examine the spatial pattern of vegetation which were located in different altitudes in Xidatan, a typical TP region, in Qinghai-Tibetan Plateau. Vegetation characteristics, soil moisture content(SMC) and thaw depths were collected in 15 August to 25 August2012. Characteristics of vegetation were mainly represented by fractional vegetation cover(FVC) derived from the normalized difference vegetation index(NDVI), as well as above ground biomass(AGB). In this paper, we analyzed that the distinction of vegetation characteristics in each range through statistics data. These ranges were divided by varied altitudes. For examples, the ranges were divided into 50 m or 100 m. In this study we use a large area plots method to further discuss the relationship between the features of vegetation and the different regions of permafrost based on altitudes shifts in Xidatan. A diagram described the vegetation characteristics variability with rising altitudes in transitional permafrost region was drawn in this paper. Our results illustrated the FVCs first increased in SFG region and then decreased in DCP zone slowly, and in CP region FVCs soared then dropped dramatically. With the altitudes increased, the curve of FVCs indicated a parabolic distribution except a little difference in the first 200 m range.展开更多
文摘In accordance with the objective to construct a major province in grassland animal husbandry,the following ways were used for enhancing development,improving the scientific and technological content,increasing the scale of the industry and market development to set up our own ecological grass industry brand and finally reach sustainable development strategy.This paper discussed the status of Guizhou Province grass industry development from the grass industry resources,grassland and animal husbandry production,prataculture technology problem.
基金supported by the National Natural Science Foundation of China (Grant No.41101055)the Hundred Talents Program of the Chinese Academy of Sciences granted to Tonghua Wu (Grant No.51Y251571)the “National Basic Research Program of China (973 Program)” (Grant No.2010CB951402)
文摘In this paper,an updated vegetation map of the permafrost zone in the Qinghai-Tibet Plateau(QTP) was delineated.The vegetation map model was extracted from vegetation sampling with remote sensing(RS) datasets by decision tree method.The spatial resolution of the map is 1 km×1 km,and in it the alpine swamp meadow is firstly distinguished in the high-altitude areas.The results showed that the total vegetated area in the permafrost zone of the QTP is 1,201,751 km^2.In the vegetated region,50,260 km^2 is the areas of alpine swamp meadow,583,909 km^2 for alpine meadow,332,754 km^2 for alpine steppe,and 234,828 km^2 for alpine desert.This updated vegetation map in permafrost zone of QTP could provide more details about the distribution of alpine vegetation types for studying the vegetation mechanisms in the land surface processes of highaltitude areas.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest"Research and Demonstration of Comprehensive Prevention Technology against Invasive Plants"(201103027)
文摘To select the suitable plant species controlling Eupatorium adenophorum in hilly area, ecological adaptability, competitiveness and control efficacy of many replacement plants with economic value in Guizhou Province of China were studied. The results showed that the coverage of paspalum wetsfeteini and Dolichos lablab were 100% and 93%, which increased by 3.70 and 3.44 times compared with Lolium perenne, respectively; their relative crowding coefficient with E. adenophorum were 7.09 and 22.78, which increased by 2.43 and 7.80 times compared with L perenroe, respectively. Using excavation method, the control efficacies of replacement plants were 99.3 % and 96.9%, respectively, while the control efficacy of replacement plants using mowing method was lower than 66.4%. The overwintering rate of P. wetsfeteini in the following year was 95% ; its coverage was still 100% and its control efficacy against E. adenophorum remained over 99%. D. lablab was difficult to survive the winter in the north region beyond 26.2°N, so it could only be applied as annual replacement plant. The coverage of Setaria anceps, Cajanus cajan and other test plants were less than 90%, with poor control efficacy against E. adenophorum.
基金the Project of the Youth Talent Development Fund of the Northwest Institute of Eco−Environment and ResourcesChinese Academy of Science(CAREERI)(Y851C81001)+1 种基金the National Natural Science Foundation of China(41877162)the Instrument Functional Development Project from the Technology Service Center of CAREERI(Y429C51007).
文摘Carex brunnescens(Pers.)Poir.is considered to be the only clonal herb found to date that can develop and form fixed dunes in Maqu alpine degraded grasslands of northwestern China.However,due to strong dormant characteristics of C.brunnescens seeds,the sand-fixing effect of the plant is severely limited.This study explores a technique that can rapidly promote the seed germination of C.brunnescens,and also investigates the adaptation and sand-fixing effect by cultivating C.brunnescens seedlings to establish living sand barriers in the sand ridges of moving sand dunes.Results show that the seed germination rate obtained a maximum of 63.7%or 65.1%when seeds were treated with 150 mg/L gibberellic acid(GA3)for 24 h followed by soaking in sulfuric acid(98%H2SO4)for 2.5 min or sodium hydroxide(10%NaOH)for 3.5 h,and then germinated(25°C in daytime and 5°C at nighttime)in darkness for 10 d.After breaking seed dormancy of C.brunnescens,the living sand barrier of C.brunnescens(plant spacing 15−20 cm;sand barrier spacing 10−20 m)was established in the perpendicular direction to the main wind in the middle and lower parts of the sand ridges on both sides of the moving sand dunes.When the sand ridges were leveled by wind erosion,the living sand barrier(plant spacing 15−20 cm;sand barrier spacing 0.5−1.0 m)of C.brunnescens was reestablished on the wind-eroded flat ground.Finally,a stable sand-fixing surface can be formed after connecting the living sand barriers on both sides,thus achieving a good sand-fixing effect.These findings suggest that rapid seed germination technology combined with the sand−fixing method of C.brunnescens can shorten the seed germination period and make the seedling establishment become much easier which may be an effective strategy to restore and reconstruct Maqu degraded grasslands.
基金Supported by the China Agriculture Research System(CARS-35-37)the Guizhou Provincial Science and Technology Foundation Project([2014]6017)
文摘The clinical signs of Tibetan sheep with sulphur- and iron-induced copper deficiency in Maqu County of Gansu Province were defined as thin, decreased appetite, neonatal ataxia, pica, emaciation, and loss of hair color and anemia. The mineral composition of soil and forage, and samples of liver, blood and hair from Tibetan sheep in affected ranches in this region were compared with those from unaffected areas. The mean concentration of iron in soil and forage in affected pasture was 19 675 ±791 and 1 763 ±197 μg/g, respectively, compared with 8 761 ±871 and 357 ±39 μg/g in unaffected pasture. The mean concentration of sulphur in soil and forage in affected pasture was 1.98% ±0.79% and 0.88% ±0.16%, respectively, compared with 1.16%±0.26% and 0.47%±0.16%in unaffected pasture. The mean copper concentrations in soil and forage from the affected pastures were 15.9±1.8 and 6.7±1.6 μg/g, respectively, compared with 16.7±1.9 and 8.9±2.7 μg/g in unaffected pasture.The mean concentration of Cu in blood, liver, and hair from the affected Tibetan sheep were 0.32±0.12, 51.2±3.1 and 2.16±0.65μg/g, respectively, compared with 0.76±0.21, 191±11 and 3.27±0.96 μg/g for healthy animals. Oral administration of CuClO(OH)3prevented and cured anemia. We conclude that wool eating of Tibetan sheep in this region is probably caused by secondary Cu deficiency, mainly due to the high iron and sulphur contents in soils and forage.
基金Supported by Guizhou Province Science and Technology Support Program(QKHZC[2016]2504,[2019]2359)
文摘[Objectives] This study was conducted to explore the interaction between nitrogen-fixing and phosphate-solubilizing strains and the optimal combination of different functional strains,in order to provide a theoretical basis for the development of PGPR compound fertilizers suitable for local environment.[Methods] In this study,16S rDNA gene sequence analysis was used to identify fast-growing and competitive strains from pasture nodules and rhizosphere soils in Guizhou Province,and three representative Rhizobia and phosphorus-solubilizing bacteria were chosen for the test of bacterial combination when reducing 50% of nitrogen and 30% of phosphorus.The effects of different strain combinations on the plant height,root length,aboveground and underground biomass of Lotus corniculatus L.were investigated,and the total nitrogen and total phosphorus contents of the plants were determined.[Results] The mixed bacterial agents could promote the increase of root biomass,and the effects of A1,A3,B3 and C3 were the most obvious.The fresh weight and dry weight of the roots of L.corniculatus increased by 30.35%-168.45% and 26.43%-180.00%,respectively,and A3,B3,B2 and C3 had the best effects.The total phosphorus content of the plants increased by 12.79%-55.25% compared with the CK2;and most of the bacterial agents with significant growth-promoting effects showed decreased total nitrogen contents,while those with non-significant growth-promoting effects showed significantly-increased total nitrogen contents,which were not as much as the CK1.Comprehensively,the most productive combination was C3,namely R27-2 Rhinohizobium fredii and P33-3 Stenotrophomonas rhizophila.[Conclusions] This study can provide a theoretical basis for the production and promotion of bacterial fertilizers.
基金Supported by Special Foundation of Guizhou Academy of Agriculture Science"Germplasm Innovation and Application of Drought and Heat Resistant Alfalfa"(QNKYYZX[2013]003)Special Breeding Project of Guizhou Agriculture Commission"Cultivation of Flowering-inhibitory Tall Fescue Varieties with RNA Interference Technique"(QNYZZ[2013]012)
文摘Abstract The metabolites of tall fescue leaves under normal and drought conditions were analyzed with LC-ESI-MS ( liquid chromatography tandem mass spec- trometry). A total of 282 metabolites were stably detected in the two groups using OPI.S-DA (orthogonal partial least squares discriminant analysis) method; 148 of which were down-regulated while 134 were up-regulated. After analyzed with MZ-minc software, 10 major metabolites (4 down, 6 up) were identified through searehing public database. Aromatic compounds, 3-p-eoumaroyll, 5-quinolaetone, aromatic free amino acids, styraxin mad methanofuran, were up-regulated ; grease metabolites, methyl 15-hydroperoxy-9Z, 12Z, 16E-octadecatrienoate, 9,12,13-Tri-HOME (10), 3-oxo-dodecanoic acid, and 13 (S)-HpOTrE acid ester, were down-regulated; piperanine (alkaloids) was down-regulated while Methyl 7-epi-12-hydroxyjasmonate glucoside (indicant) was up-regulated. In a word, aromatic compounds and liquid metabolites may play vital roles in drought-resistant regulation of tall fescue.
基金Supported by Pasture Industrialization Technology Research Integration and Application in Mountainous Areas of Guizhou([2014]6017)Phosphate Solubilizing Bacteria Bank Establishment and Phosphate Solubilization Mechanism of Pasture Rhizosphere in Mountainous Areas of Guizhou([2013]2152)
文摘The air temperature of Wuli,which is located in seasonal frozen ground zone,is rising by 13 ℃ yearly.This paper discusses the days that each ground layers' temperature lags behind the surface temperature in reaching extremum.The results were shown:The time of each ground layers' lagging days was increasing;the lagging day in warm season was longer than that in cold season;the growth rate of lagging days in warm season was 0.5 d/y,while the growth rate of lagging days in cold season was 0.7 d/y.
文摘Yunnan-Guizhou Plateau is the core area of the rocky desertification in the Chinese southwest Karst regions, and the existing studies are very limited to describe the process of rocky desertification quantitatively. The vegetation resources are applied as the key indicators in quantitative description of the degree of rocky desertification damage, and the previous methods using only remote sensing datasets are not competent to distinguish the detailed information of vegetation type, coverage and patch fragmentation on a large scale. Previous research shows that the technology of unmanned aerial vehicle and the estimation software of fraction vegetation coverages and patches could be accurately and rapidly to exploit vegetation resource information. In this project, current distribution of vegetation resources in Yunnan-Guizhou Plateau was clarified to reveal the influence mechanism of the rocky desertification. The control factors of rocky desertification distribution and the driving reasons of its dynamic changes were explained by the data of terrain, climate, population, economics and policy. Therefore, it could get the current distribution of vegetation resources in Yunnan-Guizhou Plateau, especially the grassland resources could be updated, which could not provide research foundations and scientific implications for resolving the rocky desertification, but also could provide valuable background information for the programs of restoring ecological environment and increasing local people's income in Yunnan-Guizhou Plateau.
基金Supported by Science and Technology Innovation Talents Team Foundation of Guizhou ProvinceSpecial Foundation from Guizhou Academy of Agriculture Science([2013]003)Natural Science Foundation of Guizhou Province(Qian J[2015]2080)
文摘Forages generally suffer more severe nutrition stress than other crops, but the corresponding theoretical studies still lag behind. In this study, metabo- lites in tall fescue leaves under normal and low nitrogen conditions were analyzed with LC-ESI-MS ( liquid chromatography-electrospray ionization-mass spectrome- try). A total of 1 424 and 1 251 metabolites were detected from two groups of samples respectively using OPLS-DA (orthogonal partial least squares discriminant analysis). The content of 13 major metabolites changed under low nitrogen stress, including auxin (indale acetic acid, IAA). Quantitative real-time PCR (qRT- PCR) showed that eight genes related to IAA signaling pathway were up-regulated after nitrogen stress. Enzyme-linked immuno sorbent assay (ELISA) revealed that the IAA concentration was elevated in tall fescue leaves after nitrogen stress. Our research provides valuable information for studying the response mechanisms of tall feseue to low nitrogen. The results suggest that application of IAA could be used to alleviate the harmful effects of nitrogen deficiency.
基金Supported by Outstanding Young Talent Training Program of Guizhou Province(QKHRZ[2011]1182013BAD01B03-20)
文摘In this paper, domestic and foreign advances in breeding situation and conventional breeding methods of Festuca arundinacea Schreb. and the applica- tion of modem biological techniques ( somaclonal variation, somatic cell hybridization, genetic map construction, genetic diversity, gene cloning and expression, ge- netic engineering) in breeding of new varieties of F. arundinacea were summarized, which proposed new ideas for the development of F. arundinacea breeding and provided reference for breeding of new varieties of F. arundinacea.
基金Supported by project of High-level Innovative Talents Project in Guizhou Province([2018]5634)Youth Fund of Guizhou Academy of Agricultural Sciences(2018-80)Guizhou Province S&T Contract Basics([2019]1302)
文摘The cDNA of PHYC gene of tall fescue ( Festuca arundinacea ) was cloned by RT-PCR,named FaPHYC.The sequence was analyzed by bioinformatic software,and the results showed that the full-length cDNA(3816 bp) was obtained with an open reading frame predicated to be 3 411 bp,which encoded 1 136 amino acids.By BLASTP analysis,the N-terminal of FaPHYC was composed of GAF and pPhytochrome domains,and its C-terminal included two repeated PAS domains,one histidine kinase A domain and one histidine kinase-like ATPase domain.The phylogenetic analysis showed that FaPHYC was closely related to PHYCs from other Gramineae plants.The qRT-PCR results showed that FaPHYC expression was regulated by drought stress,heat stress,salt stress and nitrogen stress.The real-time quantitative PCR assay showed the expression of FaPHYC in tall fescue seedlings was continuously up-regulated during the light period and down-regulated in the dark.The results suggest that FaPHYC is photoperiod sensitive and plays a functional role in flowering regulation by photoperiod in tall fescue.The FaPHYC gene was cloned and reported from tall fescue for the first time,which lays a foundation for the investigation of the stress mechanism of FaPHYC in tall fescue plant.
基金Supported by Outstanding Young Scientific and Technological Talent Training Fund of Guizhou Province"Study on Innovation and Application of Tall Fescue Germplasms"
文摘[ Objective ] This study aimed to construct RNAi expression vector targeting vemalizational gene FaCONSTANS (GenBank accession number: GU214996) in tall rescue. [ Method] A 145 bp long Arabidopsis actin gene intron was inserted into the expression vector to construct an intermediate vector pBI121-M-INT. Two pairs of specific primers with restriction sites were designed to amplify a 351 bp long cDNA conserved sequence fragment of vemalizational gene FaCONSTANS for RT-PCR. After restriction enzyme digestion, the amplified fragment was inserted forwardly and reversely at two sides of the intron of intermediate vector to construct an RNAi expression vector with hairpin structure. [ Result ] Double digestion (HindIII + BamHI) showed that the intron was successfully insert- ed into the vector pBI121. PCR amplification and double digestion indicated that target fragment FaCONSTANS was successfully inserted forwardly and reversely in- to the intermediate vector. [ Conclusion] This study laid foundation for breeding novel flowering-inhibited tall rescue cultivars.
基金Supported by National Natural Science Foundation of China(31260572)Special Fund for Science and Technology System Reform of Guizhou Province(Qian Ke He Z Zi[2012]4005)Guizhou Provincial Science and Technology Foundation(Qian Ke He J Zi[2013]2152)
文摘Many studies showed that permafrost has profound influence on alpine ecosystem. However, former researches were mainly focused on typical points by temporal scales. There were few studies about the correlation between vegetation characteristics and different altitudes covering a large region in spatial pattern, especially in transitional permafrost(TP). There were continuous permafrost(CP) discontinuous permafrost(DCP) and seasonal frozen ground(SFG) in this study region. The types of permafrost changed from SFG to DCP, and finally become CP as the altitudes of Xidatan increase. In this paper, 112 845 points interpreted by HJ1-B(environment and disaster monitoring and prediction small satellite constellation), vegetation investigation points, thawing layer thickness research sites, ground temperature and water content observation plots were used to examine the spatial pattern of vegetation which were located in different altitudes in Xidatan, a typical TP region, in Qinghai-Tibetan Plateau. Vegetation characteristics, soil moisture content(SMC) and thaw depths were collected in 15 August to 25 August2012. Characteristics of vegetation were mainly represented by fractional vegetation cover(FVC) derived from the normalized difference vegetation index(NDVI), as well as above ground biomass(AGB). In this paper, we analyzed that the distinction of vegetation characteristics in each range through statistics data. These ranges were divided by varied altitudes. For examples, the ranges were divided into 50 m or 100 m. In this study we use a large area plots method to further discuss the relationship between the features of vegetation and the different regions of permafrost based on altitudes shifts in Xidatan. A diagram described the vegetation characteristics variability with rising altitudes in transitional permafrost region was drawn in this paper. Our results illustrated the FVCs first increased in SFG region and then decreased in DCP zone slowly, and in CP region FVCs soared then dropped dramatically. With the altitudes increased, the curve of FVCs indicated a parabolic distribution except a little difference in the first 200 m range.