The development of optical methods in modern medicine in the areas of diagnostics,therapy,and surgery has stimulated the investigation of optical properties of various biological tissues,since the efficacy of laser tr...The development of optical methods in modern medicine in the areas of diagnostics,therapy,and surgery has stimulated the investigation of optical properties of various biological tissues,since the efficacy of laser treatment depends on the photon propagation and fluence rate distribution within irradiated tissues.In this work,an overview of published absorption and scattering properties of skin and subcutaneous tissues measured in wide wavelength range is presented.Basic principles of measurements of the tissue optical properties and techniques used for processing of the measured data are outlined.展开更多
The results on determination of glucose and glycerol difusion cofficients in myocardium tissuse are presented.The method is based on the measurement and analysis of temporal dependence of tissue optical collimated tra...The results on determination of glucose and glycerol difusion cofficients in myocardium tissuse are presented.The method is based on the measurement and analysis of temporal dependence of tissue optical collimated transmittance umder action of a hyperosmotic agent.This temporal tissue response is related to the rate of the agent and water diffusion in a tissue.The diffusion coefficients for tssue fAuid fluxes at glucose and glycerol application to the myocardium at 20℃ have been estimated as(4.75±3.40)×10^(-7) and(7.71±4.63)×10^(-7) cm^(2)/s,respectively.展开更多
Complex study of glycerol effects on the skin tissue was performed.The change in optical,weight and geometrical parameters of the rat skin under the action of the glycerol solutions was studied ex vivo.Possible mechan...Complex study of glycerol effects on the skin tissue was performed.The change in optical,weight and geometrical parameters of the rat skin under the action of the glycerol solutions was studied ex vivo.Possible mechanisms of the skin optical clearing under the action of glycerol solutions of different concentrations were discussed.The results can be helpful for refinement of models developed to evaluate the effective diffusion coefficients of glycerol in tissues.展开更多
With the objective to study the variation of optical properties of rat muscle during optical clearing,we have performed a set of optical measurements from that kind of tissue.The.measurements performed were total tr a...With the objective to study the variation of optical properties of rat muscle during optical clearing,we have performed a set of optical measurements from that kind of tissue.The.measurements performed were total tr ansmittance,ollimated transmit tance,specular reflec-tance and total reflectance.This set of measurements is suficient to determine diffuse reflectance and absorbance of the sample,also necessary to est imate the optical properties.All the per formed measurements and calculated quantities will be used later in inverse Monte Carlo(IMC)simu-lations to determine the evolution of the optical properties of muscle during treatments with ethylene glycol and glucose.The results obt ained with the measurements already provide some information about the optical c learing treatments applied to the muscle and translate the mechanisms of turning the tissue more transparent and sequence of regimes of optical clearing.展开更多
The antitumor efficiency of gold nanorod plasmonic photothermal therapy(PPTT)was evalu-ated experimentally.The rat cholangiocarcinoma line PC-1 was used as a tumor model.Exposureof tumors to S08-nm laser radiation was...The antitumor efficiency of gold nanorod plasmonic photothermal therapy(PPTT)was evalu-ated experimentally.The rat cholangiocarcinoma line PC-1 was used as a tumor model.Exposureof tumors to S08-nm laser radiation was performed,and the noninvasive temperature monitoring of the tumor tissue was carried out using infrared imager.The growth rate kinetics and mor-phological alterations of transplanted liver tumors,as wll as indicators of lipid peroxidation activity and autointoxication in rat serum,were studied,The activation of lipid peroxidation andthe development of autointoxication were detected after PPTT.The results not only demonstratethe antitumor ficacy of the proposed therapeutic technology but also reveal the side effectsin the presence of peroxidation products in systemic circulation.展开更多
The goal of this work was to evaluate the safety and efficacy of the Red Light Emitted Toothbrush(R-LETB)emitting at wavelength of 663 nm with power density of 3.3mW/cm2 in combination with 0.1%-methylene blue(MB)solu...The goal of this work was to evaluate the safety and efficacy of the Red Light Emitted Toothbrush(R-LETB)emitting at wavelength of 663 nm with power density of 3.3mW/cm2 in combination with 0.1%-methylene blue(MB)solution for the reduction of plaque and treatment of gingivitis.A microbiological in vitro study and a pilot clinical study were conducted.The microbiological study has shown total suppression of pathogenicflora after a 3-min exposure to the dye solution followed by a 20-sec treatment with the R-LETB.For the clinical study,37 subjects of both sexes with gingivitis were enrolled and randomly assigned to one of two groups.Subjects in thefirst(treatment)group were instructed to rinse their mouth with MB solution provided for 1 min and then brush the teeth with the R-LETB and standardized toothpaste.The second(control)group used only the toothpaste and a regular toothbrush.Subjects in both groups followed their respective procedures 2 times a day(morning and evening)for 30 days.Indices of plaque,gingival bleeding,and inflammation were evaluated at 14-day and 30-day timepoints.In the both groups,all indices improved in comparison with baseline.However,the treatment group demonstrated more pronounced improvement of the studied indices that was attributed to additional anti-microbial action of red light and MB on gum tissue.Thus,the use of R-LETB with MB appears to have a multifactor therapeutic action on oral pathological microflora:mechanical removal of the bacteria and suppressing action on microorganisms due to photodynamic reaction.展开更多
We are pleased to present the third issue of JIOHS,which focuses on optical technologies of diagnosticsand therapy,These are selected papers presented during Saratov Fall Meeting,1t International Sym-posium on Optics ...We are pleased to present the third issue of JIOHS,which focuses on optical technologies of diagnosticsand therapy,These are selected papers presented during Saratov Fall Meeting,1t International Sym-posium on Optics and Biophotonics(September 24-28,2013,Saratov,Russia)in the framework of the Workshops:"Optical Technologies in Biophysics_and Medicine,"Nanobiophotonics,"and"Internet Biophotonics."展开更多
One of the major challenges in imaging biological tissues using optical techniques,such as optical coherence tomography(OCT),is the lack of light penetration due to highly turbid structures within the tissue.Optical c...One of the major challenges in imaging biological tissues using optical techniques,such as optical coherence tomography(OCT),is the lack of light penetration due to highly turbid structures within the tissue.Optical clearing techniques enable the biological samples to be more optically homogeneous,allowing for deeper penetration of light into the tissue.This study investigates the effect of optical clearing utilizing various concentrations of glucose solution(10%,30%,and 50%)on porcine skin.A gold-plated mirror was imaged beneath the tissue and percentage clearing was determined by monitoring the change in reflected light intensity from the mirror over time.The ratio of percentage clearing per tissue thickness for 10%,30% and 50% glucose was determined to be 4.7±1.6%mm^(−1)(n=6),10.6±2.0%mm^(−1)(n=7)and 21.8±2.2%mm^(−1)(n=5),respectively.It was concluded that while higher glucose concentration has the highest optical clearing effect,a suitable concentration should be chosen for the purpose of clearing,considering the osmotic stress on the tissue sample.展开更多
The hollow core photonic crystal waveguide biosensor is designed and described.The biosensor was tested in experiments for artificial sweetener identifcation in drinks.The photonic crystal waveguide biosensor has a hi...The hollow core photonic crystal waveguide biosensor is designed and described.The biosensor was tested in experiments for artificial sweetener identifcation in drinks.The photonic crystal waveguide biosensor has a high sensitivity to the optical properties of liquids flling up the hollow core.The compactness,good integration ability to different optical systems and compatibility for use in industrial settings make such biosensor very promnising for v arious biomedical applicat ions.展开更多
Special Issue Optical Technologies in the Study of Tissues and Biological Fluids We are pleased to present this special issue of JIOHS,which focuses on optical technologies in the study of tissues and biological liqui...Special Issue Optical Technologies in the Study of Tissues and Biological Fluids We are pleased to present this special issue of JIOHS,which focuses on optical technologies in the study of tissues and biological liquids.The selected papers were presented during Saratov Fall Meeting—XII International School for Junior Scientists and Students on Optics,Laser Physics and Biophotonics(September 23–26,2008,Saratov,Russia)in the framework of the following workshops:“Optical Technologies in Biophysics and Medicine,”“Nanostructures and Nanoparticles,”“Microscopic and Low-Coherence Methods in Biomedical Applications,”and“Internet Biophotonics.”All eight papers in this special issue are research papers.展开更多
During the last decades, there have been significant developments in the field of biomedical photonics. We are pleased to present a special issue of Frontiers of Optoelectronics on Biomedical Photonics, which assemble...During the last decades, there have been significant developments in the field of biomedical photonics. We are pleased to present a special issue of Frontiers of Optoelectronics on Biomedical Photonics, which assembles one letter, three reviews, and eight original research articles. Optical coherence tomography (OCT) has the ability to image tissues in vivo. Polarization-sensitive optical coherence tomography (PSOCT) is a functional extension of OCT. By measuring the polarization state of light reflecting or scattering back from tissue, PSOCT enables depth-resolved mapping of sample polarization information, such as phase-retardation and optical axis orientation. In this issue, Zhenyang Ding et al. gave a review of technology developments and biomedical applications of PSOCT. Fourier domain optical coherence tomography (FDOCT) provides cross-sectional images of tissuematerial microstructures by spectral analysis of the low-coherence interference fringe pattern. Zhihua Ding et al. developed two novel systems with ultralong depth range. One is the orthogonal dispersive spectral domain optical coherence tomography (SDOCT), and the other is the recirculated swept source interferometer/OCT. No compromise between depth range and depth resolution is required in both systems. With their SDOCT system, Jian Gao et al. imaged mouse model with dorsal skin window chamber, they obtained a series of real-time OCT images and reconstructed 3D images of the specific area inside the dorsal skin window chamber by Amira.展开更多
文摘The development of optical methods in modern medicine in the areas of diagnostics,therapy,and surgery has stimulated the investigation of optical properties of various biological tissues,since the efficacy of laser treatment depends on the photon propagation and fluence rate distribution within irradiated tissues.In this work,an overview of published absorption and scattering properties of skin and subcutaneous tissues measured in wide wavelength range is presented.Basic principles of measurements of the tissue optical properties and techniques used for processing of the measured data are outlined.
基金support from grants:13-02-91176 of Russian Foundation for Basic Researchgrant No.14.Z50.31.0004 by Government of the Russian FederationRussian Presidential grant NSh-703.2014.2.
文摘The results on determination of glucose and glycerol difusion cofficients in myocardium tissuse are presented.The method is based on the measurement and analysis of temporal dependence of tissue optical collimated transmittance umder action of a hyperosmotic agent.This temporal tissue response is related to the rate of the agent and water diffusion in a tissue.The diffusion coefficients for tssue fAuid fluxes at glucose and glycerol application to the myocardium at 20℃ have been estimated as(4.75±3.40)×10^(-7) and(7.71±4.63)×10^(-7) cm^(2)/s,respectively.
基金This work was carried out under the support from the Russian Foundation for Basic Research(Grant Nos.18-52-16025 and 19-32-90224)The Fund for Promoting Innovation grant UMNIK-19/HealthNet.NTI-2019 No.15929GU/2020 of 07.23.2020(code 0059878,application(U-65096)).
文摘Complex study of glycerol effects on the skin tissue was performed.The change in optical,weight and geometrical parameters of the rat skin under the action of the glycerol solutions was studied ex vivo.Possible mechanisms of the skin optical clearing under the action of glycerol solutions of different concentrations were discussed.The results can be helpful for refinement of models developed to evaluate the effective diffusion coefficients of glycerol in tissues.
基金supported in part by grants:RF Governmental contracts 11.519.11.2035,14.B37.21.0728,and 14.B37.11.0563FiDiPro,TEKES Program(40111/11),Finland+1 种基金SCOPES EC,Uzb/Switz/RF,Swiss NSF,IZ74ZO 137423/1RF President's grant"Scientific Schools",1177.2012.2.
文摘With the objective to study the variation of optical properties of rat muscle during optical clearing,we have performed a set of optical measurements from that kind of tissue.The.measurements performed were total tr ansmittance,ollimated transmit tance,specular reflec-tance and total reflectance.This set of measurements is suficient to determine diffuse reflectance and absorbance of the sample,also necessary to est imate the optical properties.All the per formed measurements and calculated quantities will be used later in inverse Monte Carlo(IMC)simu-lations to determine the evolution of the optical properties of muscle during treatments with ethylene glycol and glucose.The results obt ained with the measurements already provide some information about the optical c learing treatments applied to the muscle and translate the mechanisms of turning the tissue more transparent and sequence of regimes of optical clearing.
基金supported by a grant No.14-13-01167 from the Russian Scientic Foundationsupported by the Russian Presidential grant NSh-703.2014.2 (ANB,EAG and VVT)by ABB,GNM and GST was supported by a grant No.14.Z50.31.0004 to support scientic research projects implemented under the supervision of leading scientists at Russian institutions of higher education.
文摘The antitumor efficiency of gold nanorod plasmonic photothermal therapy(PPTT)was evalu-ated experimentally.The rat cholangiocarcinoma line PC-1 was used as a tumor model.Exposureof tumors to S08-nm laser radiation was performed,and the noninvasive temperature monitoring of the tumor tissue was carried out using infrared imager.The growth rate kinetics and mor-phological alterations of transplanted liver tumors,as wll as indicators of lipid peroxidation activity and autointoxication in rat serum,were studied,The activation of lipid peroxidation andthe development of autointoxication were detected after PPTT.The results not only demonstratethe antitumor ficacy of the proposed therapeutic technology but also reveal the side effectsin the presence of peroxidation products in systemic circulation.
基金The authors are grateful to Palomar Medical Technologies,Inc.(Burlington,MA,USA)for funding this work.The research described in this paper was also supported by grant No.224014 PHOTONICS4LIFE of FP7-ICT-2007-2(2008-2013)Governmental contracts 02.740.11.0484 and 02.740.11.0879.
文摘The goal of this work was to evaluate the safety and efficacy of the Red Light Emitted Toothbrush(R-LETB)emitting at wavelength of 663 nm with power density of 3.3mW/cm2 in combination with 0.1%-methylene blue(MB)solution for the reduction of plaque and treatment of gingivitis.A microbiological in vitro study and a pilot clinical study were conducted.The microbiological study has shown total suppression of pathogenicflora after a 3-min exposure to the dye solution followed by a 20-sec treatment with the R-LETB.For the clinical study,37 subjects of both sexes with gingivitis were enrolled and randomly assigned to one of two groups.Subjects in thefirst(treatment)group were instructed to rinse their mouth with MB solution provided for 1 min and then brush the teeth with the R-LETB and standardized toothpaste.The second(control)group used only the toothpaste and a regular toothbrush.Subjects in both groups followed their respective procedures 2 times a day(morning and evening)for 30 days.Indices of plaque,gingival bleeding,and inflammation were evaluated at 14-day and 30-day timepoints.In the both groups,all indices improved in comparison with baseline.However,the treatment group demonstrated more pronounced improvement of the studied indices that was attributed to additional anti-microbial action of red light and MB on gum tissue.Thus,the use of R-LETB with MB appears to have a multifactor therapeutic action on oral pathological microflora:mechanical removal of the bacteria and suppressing action on microorganisms due to photodynamic reaction.
文摘We are pleased to present the third issue of JIOHS,which focuses on optical technologies of diagnosticsand therapy,These are selected papers presented during Saratov Fall Meeting,1t International Sym-posium on Optics and Biophotonics(September 24-28,2013,Saratov,Russia)in the framework of the Workshops:"Optical Technologies in Biophysics_and Medicine,"Nanobiophotonics,"and"Internet Biophotonics."
基金the Institute of Biomedical Imaging Sciences(IBIS-97708)CRDF(RUB1-2932-SR-08)+3 种基金NSF(CMMI-0900743)supported by grants 224014 Photonics4life-FP7-ICT-2007-2RF Ministry of Science and Education 2.1.1/4989 and 2.2.1.1/2950,Project 1.4.09 of Federal Agency of Education of RFthe RF Governmental contracts 02.740.11.0484 and 02.740.11.0770.
文摘One of the major challenges in imaging biological tissues using optical techniques,such as optical coherence tomography(OCT),is the lack of light penetration due to highly turbid structures within the tissue.Optical clearing techniques enable the biological samples to be more optically homogeneous,allowing for deeper penetration of light into the tissue.This study investigates the effect of optical clearing utilizing various concentrations of glucose solution(10%,30%,and 50%)on porcine skin.A gold-plated mirror was imaged beneath the tissue and percentage clearing was determined by monitoring the change in reflected light intensity from the mirror over time.The ratio of percentage clearing per tissue thickness for 10%,30% and 50% glucose was determined to be 4.7±1.6%mm^(−1)(n=6),10.6±2.0%mm^(−1)(n=7)and 21.8±2.2%mm^(−1)(n=5),respectively.It was concluded that while higher glucose concentration has the highest optical clearing effect,a suitable concentration should be chosen for the purpose of clearing,considering the osmotic stress on the tissue sample.
基金supported by Grant No.224014 PHOTONICS4LIFE of FP7-ICT-2007-2Project No.1.4.09+3 种基金RF Governmental contracts 11.519.11.2035,14.B37.21.0728 and 14.B37.21.0563FiDiPro,TEKES Program(40111/11),FinlandSCOPES EC,Uzb/Switz/RF,Swiss NSF,IZ74ZO 137423/1RF President's Grant 1177.2012.2"Scientific Schools".
文摘The hollow core photonic crystal waveguide biosensor is designed and described.The biosensor was tested in experiments for artificial sweetener identifcation in drinks.The photonic crystal waveguide biosensor has a high sensitivity to the optical properties of liquids flling up the hollow core.The compactness,good integration ability to different optical systems and compatibility for use in industrial settings make such biosensor very promnising for v arious biomedical applicat ions.
文摘Special Issue Optical Technologies in the Study of Tissues and Biological Fluids We are pleased to present this special issue of JIOHS,which focuses on optical technologies in the study of tissues and biological liquids.The selected papers were presented during Saratov Fall Meeting—XII International School for Junior Scientists and Students on Optics,Laser Physics and Biophotonics(September 23–26,2008,Saratov,Russia)in the framework of the following workshops:“Optical Technologies in Biophysics and Medicine,”“Nanostructures and Nanoparticles,”“Microscopic and Low-Coherence Methods in Biomedical Applications,”and“Internet Biophotonics.”All eight papers in this special issue are research papers.
文摘During the last decades, there have been significant developments in the field of biomedical photonics. We are pleased to present a special issue of Frontiers of Optoelectronics on Biomedical Photonics, which assembles one letter, three reviews, and eight original research articles. Optical coherence tomography (OCT) has the ability to image tissues in vivo. Polarization-sensitive optical coherence tomography (PSOCT) is a functional extension of OCT. By measuring the polarization state of light reflecting or scattering back from tissue, PSOCT enables depth-resolved mapping of sample polarization information, such as phase-retardation and optical axis orientation. In this issue, Zhenyang Ding et al. gave a review of technology developments and biomedical applications of PSOCT. Fourier domain optical coherence tomography (FDOCT) provides cross-sectional images of tissuematerial microstructures by spectral analysis of the low-coherence interference fringe pattern. Zhihua Ding et al. developed two novel systems with ultralong depth range. One is the orthogonal dispersive spectral domain optical coherence tomography (SDOCT), and the other is the recirculated swept source interferometer/OCT. No compromise between depth range and depth resolution is required in both systems. With their SDOCT system, Jian Gao et al. imaged mouse model with dorsal skin window chamber, they obtained a series of real-time OCT images and reconstructed 3D images of the specific area inside the dorsal skin window chamber by Amira.