Recently,increasing attention has been devoted to mastering a new technique of optical delivery of micro-objects tractorbeam’1–9.Such beams have uniform intensity profiles along their propagation direction and can e...Recently,increasing attention has been devoted to mastering a new technique of optical delivery of micro-objects tractorbeam’1–9.Such beams have uniform intensity profiles along their propagation direction and can exert a negative force that,in contrast to the familiar pushing force associated with radiation pressure,pulls the scatterer toward the light source.It was experimentally observed that under certain circumstances,the pulling force can be significantly enhanced6 if a non-spherical scatterer,for example,a linear chain of optically bound objects10–12,is optically transported.Here we demonstrate that motion of two optically bound objects in a tractor beam strongly depends on theirs mutual distance and spatial orientation.Such configuration-dependent optical forces add extra flexibility to our ability to control matter with light.Understanding these interactions opens the door to new applications involving the formation,sorting or delivery of colloidal self-organized structures.展开更多
Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system1–4.Advances...Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system1–4.Advances in wavefrontshaping methods and computational power have recently allowed for a novel approach to high-resolution imaging,utilizing deterministic light propagation through optically complex media and,of particular importance for this work,multimode optical fibers(MMFs)5–7.We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications.The volume of tissue lesion was reduced by more than 100-fold,while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-μm-core MMF.Here,we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures,dendrites and synaptic specializations,in deep-brain regions of living mice,as well as monitored stimulus-driven functional Ca2+responses.These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage,heralding new possibilities for deep-brain imaging in vivo.展开更多
基金supported by the projects of CSF(GA14-16195S),TACR(TE01020233)its infrastructure by MEYS CR,EC,and CAS(LO1212,CZ.1.05/2.1.00/01.0017,RVO:68081731)supported by Brno Ph.D.Talent program。
文摘Recently,increasing attention has been devoted to mastering a new technique of optical delivery of micro-objects tractorbeam’1–9.Such beams have uniform intensity profiles along their propagation direction and can exert a negative force that,in contrast to the familiar pushing force associated with radiation pressure,pulls the scatterer toward the light source.It was experimentally observed that under certain circumstances,the pulling force can be significantly enhanced6 if a non-spherical scatterer,for example,a linear chain of optically bound objects10–12,is optically transported.Here we demonstrate that motion of two optically bound objects in a tractor beam strongly depends on theirs mutual distance and spatial orientation.Such configuration-dependent optical forces add extra flexibility to our ability to control matter with light.Understanding these interactions opens the door to new applications involving the formation,sorting or delivery of colloidal self-organized structures.
基金support from the University of Dundee and Scottish Universities Physics Alliance(PaLS initiative)support from the European Regional Development Fund,Project No.CZ.02.1.01/0.0/0.0/15003/0000476+1 种基金support from the John Fell Fund,the BBSRC(TDRF)the MRC(UK).
文摘Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system1–4.Advances in wavefrontshaping methods and computational power have recently allowed for a novel approach to high-resolution imaging,utilizing deterministic light propagation through optically complex media and,of particular importance for this work,multimode optical fibers(MMFs)5–7.We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications.The volume of tissue lesion was reduced by more than 100-fold,while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-μm-core MMF.Here,we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures,dendrites and synaptic specializations,in deep-brain regions of living mice,as well as monitored stimulus-driven functional Ca2+responses.These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage,heralding new possibilities for deep-brain imaging in vivo.
