Effects of Fertilization on Soil CO_(2) Efflux in Chinese Hickory(Carya cathayensis)Stands

Chinese hickory(Carya cathayensis Sarg.)is a popular nut tree in China,but there is little information about the influences of fertilization on soil CO_(2) efflux and soil microbial biomass.This study evaluated the short-term effects of different fertilizer applications on soil CO_(2) efflux and soil microbial biomass in Chinese hickory stands.Four fertilizer treatments were established:control(CK,no fertilizer),inorganic fertilizer(IF),organic fertilizer(OF),and equal parts organic and inorganic N fertilizers(OIF).A field experiment was conducted to measure soil CO_(2) effluxes using closed chamber and gas chromatography techniques.Regardless of the fertilization practices,soil CO_(2) effluxes of all the treatments showed a similar temporal pattern,with the highest value in summer and the lowest in winter.The mean annual soil CO_(2) efflux in the IF treatment was significantly higher than that in the CK,OIF,and OF treatments.There was no significant difference in soil CO_(2) efflux between the OIF,OF,and CK treatments.Soil CO_(2) effluxes were significantly affected by soil temperature.Soil dissolved organic carbon(DOC)was positively correlated with soil CO_(2) efflux only in the CK treatment.Regression analysis,including soil temperature,moisture,and DOC,showed that soil temperature was the primary factor influencing soil CO_(2) effluxes.Both OF and OIF treatments increased concentrations of soil microbial biomass carbon(MBC)and microbial biomass nitrogen(MBN),but decreased the ratio of MBC:MBN.These results reveal that applying organic fertilizer,either alone or combined with inorganic fertilizer,may be the optimal strategy for mitigating soil CO_(2) emission and improving soil quality in Chinese hickory stands.

Straw return increases crop production by improving soil organic carbon sequestration and soil aggregation in a long-term wheat-cotton cropping system

Straw return is a promising strategy for managing soil organic carbon(SOC)and improving yield stability.However,the optimal straw return strategy for sustainable crop production in the wheat(Triticum aestivum L.)-cotton(Gossypium hirsutum L.)cropping system remains uncertain.The objective of this study was to quantify the long-term(10 years)impact of carbon(C)input on SOC sequestration,soil aggregation and crop yields in a wheat-cotton cropping system in the Yangtze River Valley,China.Five treatments were arranged with a single-factor randomized design as follows:no straw return(Control),return of wheat straw only(Wt),return of cotton straw only(Ct),return of 50%wheat and 50%cotton straw(Wh-Ch)and return of 100%wheat and 100%cotton straw(Wt-Ct).In comparison to the Control,the SOC content increased by 8.4 to 20.2%under straw return.A significant linear positive correlation between SOC sequestration and C input(1.42-7.19 Mg ha^(−1)yr^(−1))(P<0.05)was detected.The percentages of aggregates of sizes>2 and 1-2 mm at the 0-20 cm soil depth were also significantly elevated under straw return,with the greatest increase of the aggregate stability in the Wt-Ct treatment(28.1%).The average wheat yields increased by 12.4-36.0%and cotton yields increased by 29.4-73.7%,and significantly linear positive correlations were also detected between C input and the yields of wheat and cotton.The average sustainable yield index(SYI)reached a maximum value of 0.69 when the C input was 7.08 Mg ha^(−1)yr^(−1),which was close to the maximum value(SYI of 0.69,C input of 7.19 Mg ha^(−1)yr^(-1))in the Wt-Ct treatment.Overall,the return of both wheat and cotton straw was the best strategy for improving SOC sequestration,soil aggregation,yields and their sustainability in the wheat-cotton rotation system.

Cell Wall Fixation,Translocation,and Vacuolar Detoxification of Cadmium Contribute to Differential Grain Cadmium Accumulation in Two Rice Cultivars

Cadmium(Cd)toxicity in rice is a major concern for human health and the environment,as it can accumulate in rice grains when grown in Cd-contaminated soils.To mitigate the risk of Cd toxicity,it is crucial to cultivate rice varieties with low grain Cd accumulation.In the summers of 2021 and 2022,we conducted Cd analysis on two rice cultivars,Tianyouhuazhan(TYHZ)and Xiushui 14,grown in fields with varying Cd pollution levels.These cultivars were also subjected to hydroponic treatment with or without 1μmol/L Cd for 7 d to assess Cd accumulation,nitric oxide(NO)production。

Effects of Tillage Depth on Nutrients and Microbial Communities in Tobacco-Planting Soil

The implementation of appropriate tillage practices is of great significance for agricultural production. However, the effects of different tillage depths on soil nutrients content and microbial communities in tobacco-planting soils are still lacking systematic research. In this study, three different tillage depths of 15 cm (T1), 20 cm (T2), and 30 cm (T3) were set up for field experiments in Liupanshui, Guizhou Province, to explore the effects of tillage depth on tobacco-planting soil nutrients and bacterial and fungal communities based on 16S rRNA and ITS sequencing and figure out the key factors affecting soil microbial communities. The results showed that T2 and T3 increased the contents of organic matter, total nitrogen, total phosphorus, available phosphorus, and available potassium in tobacco-planting soil, and increased the diversity of bacterial communities compared with T1. There was no significant difference in the structure of bacterial and fungal communities in different tillage depth treatments, but some dominant genera were significantly enriched in T2 and T3. Desulfobacter, Setophoma, Humicola, and Acremonium were significantly enriched in T2. Chthonomonas and Fusarium were significantly enriched in T3. These genera favor the decomposition of organic matter and the cycling of nutrients, and control soil pests and diseases. Redundancy analysis indicated that TP and AK were the key factors influencing the dominant genera of bacteria and fungi. This study provides a scientific basis for the selection of soil tillage depth for tobacco production in this region.

Mapping soil organic matter in cultivated land based on multi-year composite images on monthly time scales

Rapid and accurate acquisition of soil organic matter(SOM)information in cultivated land is important for sustainable agricultural development and carbon balance management.This study proposed a novel approach to predict SOM with high accuracy using multiyear synthetic remote sensing variables on a monthly scale.We obtained 12 monthly synthetic Sentinel-2 images covering the study area from 2016 to 2021 through the Google Earth Engine(GEE)platform,and reflectance bands and vegetation indices were extracted from these composite images.Then the random forest(RF),support vector machine(SVM)and gradient boosting regression tree(GBRT)models were tested to investigate the difference in SOM prediction accuracy under different combinations of monthly synthetic variables.Results showed that firstly,all monthly synthetic spectral bands of Sentinel-2 showed a significant correlation with SOM(P<0.05)for the months of January,March,April,October,and November.Secondly,in terms of single-monthly composite variables,the prediction accuracy was relatively poor,with the highest R^(2)value of 0.36 being observed in January.When monthly synthetic environmental variables were grouped in accordance with the four quarters of the year,the first quarter and the fourth quarter showed good performance,and any combination of three quarters was similar in estimation accuracy.The overall best performance was observed when all monthly synthetic variables were incorporated into the models.Thirdly,among the three models compared,the RF model was consistently more accurate than the SVM and GBRT models,achieving an R^(2)value of 0.56.Except for band 12 in December,the importance of the remaining bands did not exhibit significant differences.This research offers a new attempt to map SOM with high accuracy and fine spatial resolution based on monthly synthetic Sentinel-2 images.

Potential Secretory Transporters and Biosynthetic Precursors of Biological Nitrification Inhibitor 1,9-Decanediol in Rice as Revealed by Transcriptome and Metabolome Analyses

Biological nitrification inhibitors(BNIs)are released from plant roots and inhibit the nitrification activity of microorganisms in soils,reducing NO_(3)^(‒)leaching and N2O emissions,and increasing nitrogenuse efficiency(NUE).Several recent studies have focused on the identification of new BNIs,yet little is known about the genetic loci that govern their biosynthesis and secretion.We applied a combined transcriptomic and metabolomic analysis to investigate possible biosynthetic pathways and transporters involved in the biosynthesis and release of BNI 1,9-decanediol(1,9-D),which was previously identified in rice root exudates.Our results linked four fatty acids,icosapentaenoic acid,linoleate,norlinolenic acid,and polyhydroxy-α,ω-divarboxylic acid,with 1,9-D biosynthesis and three transporter families,namely the ATP-binding cassette protein family,the multidrug and toxic compound extrusion family,and the major facilitator superfamily,with 1,9-D release from roots into the soil medium.Our finding provided candidates for further work on the genes implicated in the biosynthesis and secretion of 1,9-D and pinpoint genetic loci for crop breeding to improve NUE by enhancing 1,9-D secretion,with the potential to reduce NO_(3)^(‒)leaching and N2O emissions from agricultural soils.

Putrescine Modulates Cadmium Fixation Ability of Cell Wall to Decrease Cadmium Accumulation in Rice,a Process Might Depend on Nitric Oxide

Although putrescine(PUT)has been linked to plants'responses to cadmium(Cd)stress,the exact mechanism is still elusive.In the current investigation,the endogenous PUT concentration in rice roots was enhanced by Cd stress.Exogenous PUT increased root cell wall hemicellulose levels,which in turn increased its Cd binding capacity,concurrently decreasing the transcription level of genes such as OsNRAMP1 and OsCd1 that are responsible for root Cd absorption.As a result。

Planetary Homeostasis of Reactive Nitrogen Through Anaerobic Ammonium Oxidation

The availability of nitrogen(N)is crucial for both the productivity of terrestrial and aquatic ecosystems globally.However,the overuse of artificial fertilizers and the energy required to fix nitrogen have pushed the global nitrogen cycle(N-cycle)past its safe operating limits,leading to severe nitrogen pollution and the production of significant amounts of greenhouse gas nitrous oxide(N2O).The anaerobic ammonium oxidation(anammox)mechanism can counteract the release of ammonium and N2O in many oxygenlimited situations,assisting in the restoration of the homeostasis of the Earth’s N biogeochemistry.In this work,we looked into the characteristics of the anammox hotspots’distribution across various types of ecosystems worldwide.Anammox hotspots are present at diverse oxic-anoxic interfaces in terrestrial systems,and they are most prevalent at the oxic-anoxic transition zone in aquatic ecosystems.Based on the discovery of an anammox hotspot capable of oxidizing ammonium anoxically into N2 without N2O by-product,we then designed an innovative concept and technical routes of nature-based anammox hotspot geoengineering for climate change,biodiversity loss,and efficient utilization of water resources.After 15 years of actual use,anammox hotspot geoengineering has proven to be effective in ensuring clean drinking water,regulating the climate,fostering plant and animal diversity,and enhancing longterm environmental quality.The sustainable biogeoengineering of anammox could be a workable natural remedy to resolve the conflicts between environmental pollution and food security connected to N management.

Fertilization and Soil Ploughing Practices under Changing Physical Environment Lead to Soil Organic Carbon Dynamics under Conservation Agriculture in Rice-Wheat Cropping System: A Scoping Review

Ploughing and fertilization practices in rice-wheat system have deteriorated the soil carbon (C) pools. Conservation agriculture (CA) based management approaches have proven to enhance C sequestration and reverse the loss of soil-organic-carbon (SOC), which further enhances soil fertility. Different fractions of SOC pools react to the alterations in management practices and indicate changes in SOC dynamics as compared to total C in the soil. Higher SOC levels in soil have been observed in case of reduced/no-till (NT) practices than conventional tillage (CT). However, between CT and zero tillage/NT, total SOC stocks diminished with an increase in soil depth, which demonstrated that the benefits of SOC are more pronounced in the topsoil under NT. Soil aggregation provides physical protection to C associated with different-sized particles, thus, the improvement in soil aggregation through CA is an effective way to mitigate soil C loss. Along with less soil disturbance, residual management, suitable crop rotation, rational application of manures and fertilizers, and integrated nutrient management have been found to be effective in not only improving soil C stock but also enhancing the soil health and productivity. Thus, CA can be considered as a potential method in the build-up of SOC of soil in rice-wheat system.

Effects of several amendments on rice growth and uptake of copper and cadmium from a contaminated soil

Heavy metals in variable charge soil are highly bioavailable and easy to transfer into plants. Since it is impossible to completely eliminate rice planting on contaminated soils, some remediation and mitigation techniques are necessary to reduce metal bioavailability and uptake by rice. This pot experiment investigated the effects of seven amendments on the growth of rice and uptake of heavy metals from a paddy soil that was contaminated by copper and cadmium. The best results were from the application of limestone that increased grain yield by 12.5-16.5 fold, and decreased Cu and Cd concentrations in grain by 23.0%--50.4%. Application of calcium magnesium phosphate, calcium silicate, pig manure, and peat also increased the grain yield by 0.3-15.3 fold, and effectively decreased the Cu and Cd concentrations in grain. Cd concentration in grain was slightly reduced in the treatments of Chinese milk vetch and zinc sulfate. Concentrations of Cu and Cd in grain and straw were dependent on the available Cu and Cd in the soils, and soil available Cu and Cd were significantly affected by the soil pH.

Effect of Long-Term Rice Straw Return on Soil Glomalin, Carbon and Nitrogen

A long-term experiment was conducted to investigate how long-term fertilization and rice straw incorporation into soil affect soil glomalin, C and N. The combined application of chemical fertilizer and straw resulted in a significant increase in both soil easily extractable glomalin (EEG) and total glomalin (TG) concentrations, as compared with application of only chemical fertilizer or no fertilizer application. The EEG and TG concentrations of the NPKS (nitrogen, phosphorus, and potassium fertilizer application + rice straw return) plot were 4.68% and 5.67% higher than those of the CK (unfertilized control) plot, and 9.87% and 6.23% higher than those of the NPK (nitrogen, phosphorus, and potassium fertilizer applied annually) plot, respectively. Application of only chemical fertilizer did not cause a statistically significant change of soil glomalin compared with no fertilizer application. The changes of soil organic C (SOC) and total N (TN) contents demonstrated a similar trend to soil glomalin in these plots. The SOC and TN contents of NPKS plot were 15.01% and 9.18% higher than those of the CK plot, and 8.85% and 14.76% higher than those of the NPK plot, respectively. Rice straw return also enhanced the contents of microbial biomass C (MBC) and microbial biomass N (MBN) in the NPKS plot by 7.76% for MBC and 31.42% for MBN compared with the CK plot, and 12.66% for MBC and 15.07% for MBN compared with the NPK plots, respectively. Application of only chemical fertilizer, however, increased MBN concentration, but decreased MBC concentration in soil.

Paddy Soil Stability and Mechanical Properties as Affected by Long-Term Application of Chemical Fertilizer and Animal Manure in Subtropical China

Wet stability, penetration resistance （PR）, and tensile strength （TS） of paddy soils under a fertilization experiment for 22 years were determined to elucidate the function of soil organic matter in paddy soil stabilization. The treatments included no fertilization （CK）, normal chemical fertilization （NPK）, double the NPK application rates （2NPK）, and NPK mixed with organic manure （NPK＋OM）. Compared with CK, Fertilization increased soil organic carbon （SOC） and soil porosity. The results of soil aggregate fragmentation degree （SAFD） showed that fast wetting by water was the key fragmentation mechanism. Among the treatments, the NPK＋OM treatment had the largest size of water-stable aggregates and greatest normal mean weight diameter （NMWD） （P ≤ 0.05）, but the lowest PR and TS in both cultivated horizon （Ap） and plow pan. The CK and 2NPK treatments were measured with PR 〉 2.0 MPa and friability index 〈 0.20, respectively, in the Ap horizon, suggesting that the soils was mechanically unfavourable to root growth and tillage. In the plow pan, the fertilization treatments had greater TS and PR than in CK. TS and PR of the tested soil aggregates were negatively correlated to SOC content and soil porosity. This study suggested that chemical fertilization could cause deterioration of mechanical properties while application of organic manure could improve soil stability and mechanical properties.

Cross-Reference Benchmarks for Translating the Genetic Soil Classification of China into the Chinese Soil Taxonomy

Soil classification is the foundation for exchange and extension of research findings in soil science and for modern management of soil resources. This study explained database and research methodology to create a cross-reference system for translating the Genetic Soil Classification of China (GSCC) into the Chinese Soil Taxonomy (CST). With the help of the CST keys, each of the 2 540 soil species in GSCC has been interpreted to its corresponding soil order, suborder, great group, and sub-group in CST. According to the methodology adopted, the assigned soil species have been linked one another to their corresponding polygons in the 1:1000000 digital soil map of China. Referencibility of each soil species between the GSCC and CST systems was determined statistically on the basis of distribution area of each soil species at a high taxon level of the two systems. The soils were then sorted according to their maximum referencibility and classified into three categories for discussion. There were 19 soil great groups in GSCC with maximum referencibility > 90% and 22 great groups between 60%-90%. These soil great groups could serve as cross-reference benchmarks. There were 19 great groups in GSCC with maximum referencibility < 60%, which could be used as cross-reference benchmarks until new and better results were available. For these soils, if the translation was made at a lower soil taxon level or on a regional basis, it would improve their referencibility enabling them to serve as new cross-reference benchmarks.

National Scale Analysis of Soil Organic Carbon Storage in China Based on Chinese Soil Taxonomy

Patterns of soil organic carbon (SOC) storage and density in various soil types or locations are the foundation for examining the role of soil in the global carbon cycle. An assessment of SOC storage and density patterns in China based on soil types as defined by Chinese Soil Taxonomy (CST) and the recently compiled digital 1:1000000 Soil Database of China was conducted to generate a rigorous database for the future study of SOC storage. First, SOC densities of 7 292 soil profiles were calculated and linked by soil type to polygons of a digital soil map using geographic information system resulting in a 1:1 000 000 SOC density distribution map of China. Further results showed that soils in China covered 9 281×103 km2 with a total SOC storage of 89.14 Gt and a mean SOC density 96.0 t ha-1. Among the 14 CST orders, Cambosols and Argosols constituted high percentage of China's total SOC storage, while Andosols, Vertosols, and Spodsols had a low percentage. As for SOC density, Histosols were the highest, while Primosols were the lowest. Specific patterns of SOC storage of various soil types at the CST suborder, group, and subgroup levels were also described. Results obtained from the study of SOC storage and density of all CST soil types would be not only useful for international comparative research, but also for more accurately estimating and monitoring of changes of SOC storage in China.

Adsorption and desorption of Cu(Ⅱ) and Pb(Ⅱ) in paddy soils cultivated for various years in the subtropical China

The adsorption and desorption of Cu（Ⅱ） and Pb（Ⅱ） on upland red soil,and paddy soils which were originated from the upland soil and cultivated for 8,15,35 and 85 years,were investigated using the batch method.The study showed that the organic matter content and cation exchange capacity （CEC） of the soils are important factors controlling the adsorption and desorption of Cu（Ⅱ） and Pb（Ⅱ）.The 15-Year paddy soil had the highest adsorption capacity for Pb（Ⅱ）,followed by the 35-Year paddy soil.Both the 35-Year paddy soil and 15-Year paddy soil adsorbed more Cu（Ⅱ） than the upland soil and other paddy soils.The 15-Year paddy soils exhibited the highest desorption percentage for both Cu（Ⅱ） and Pb（Ⅱ）.These results are consistent with the trend for the CEC of the soils tested.The high soil CEC contributes not only to the adsorption of Cu（Ⅱ） and Pb（Ⅱ） but also to the electrostatic adsorption of the two heavy metals by the soils.Lower desorption percentages for Cu（Ⅱ） （36.7% to 42.2%） and Pb（Ⅱ） （50.4% to 57.9%） were observed for the 85-Year paddy soil.The highest content of organic matter in the soil was responsible for the low desorption percentages for the two metals because the formation of the complexes between the organic matter and the metals could increase the stability of the heavy metals in the soils.

Effects of Long-Term Application of Inorganic Fertilizers on Biochemical Properties of a Rice-Planting Red Soil

A long-term experiment was set up in Yingtan of Jiangxi Province to investigate the effects of long-term application of inorganic fertilizers on the biochemical properties of a rice-planting soil derived from Quaternary red earth. Noncultivated soils are extremely eroded and characterized by a low pH and deficiencies in available nutrients, in particular P and N. After 13 years of inorganic fertilization in cultivation for double-cropped rice, the biochemical properties of the soil were changed. The nitrification potential and urease activity were higher in the treatments with N application than those without N application, Acid phosphatase activity and dehydrogenase activity were also higher in the treatments with P application than in those without P application. The dehydrogenase activity correlated well with the concentrations of both total P and hydrolysable N and with rice crop yield, suggesting that dehydrogenase activity might be a suitable indicator for improvement in soil fertility.

Changes in Soil C and N Contents and Mineralization Across a Cultivation Chronosequence of Paddy Fields in Subtropical China

Dynamics of soil organic matter in a cultivation chronosequence of paddy fields were studied in subtropical China.Mineralization of soil organic matter was determined by measuring CO2 evolution from soil during 20 days of laboratoryincubation. In the first 30 years of cultivation, soil organic C and N contents increased rapidly. After 30 years, 0-10 cmsoil contained 19.6 g kg-1 organic C and 1.62 g kg-1 total N, with the corresponding values of 18.1 g kg-1 and 1.50g kg-1 for 10-20 cm, and then remained stable even after 80 years of rice cultivation. During 20 days incubation themineralization rates of organic C and N in surface soil (0-10 cm) ranged from 2.2% to 3.3% and from 2.8% to 6.7%,respectively, of organic C and total N contents. Biologically active C size generally increased with increasing soil organicC and N contents. Soil dissolved organic C decreased after cultivation of wasteland to 10 years paddy field and thenincreased. Soil microbial biomass C increased with number of years under cultivation, while soil microbial biomass Nincreased during the first 30 years of cultivation and then stabilized. After 30 years of cultivation surface soil (0-10 cm)contained 332.8 mg kg-1 of microbial biomass C and 23.85 mg kg-1 of microbial biomass N, which were 111% and 47%higher than those in soil cultivated for 3 years. It was suggested that surface soil with 30 years of rice cultivation insubtropical China would have attained a steady state of organic C content, being about 19 g kg-1.

Spatial Distribution of Heavy Metals in Agricultural Soils of an Industry-Based Peri-Urban Area in Wuxi, China

In industry-oriented peri-urban areas, the heavy metal accumulation in soils caused by industrialization has become a potential threat. The top soil samples from 27 paddy fields and 75 vegetable fields were collected from a typical industry- based peri-urban area of about 8 km^2 in Wuxi, China, to study the accumulation and distribution of As, Hg, Cu, Zn, Pb, Cr, and Cd in comparison with heavy metal contents in soils near developed industrial sites （Guangzhou, China; Wallsend Burn of Tyneside, UK; and Osnabrück, Germany）. Kriging interpolation was used to determine the metals, spatial distribution. The results showed that most soils, compared to the background values, contained elevated contents of As, Hg, Cu, Zn, and Pb with some having elevated contents of Cd and Cr. Except for less than 10% of the soil samples of Cu, Zn and Cd contents, these heavy metal contents were lower than the soil threshold levels of the Grade Ⅱ criteria for the Chinese environmental quality standard. Probably, because of the scattered distribution and diversity of industries in the study area, spatial distributions of these heavy metals from Kriging interpolation indicated little similarity. Nevertheless, when compared with other areas in the Taihu Lake region, mean contents of Cu, Zn, Pb, and Cd were relatively high in the Wuxi peri-urban area. Additionally, compared to soils in agricultural areas around Guangzhou, Osnabrück, or Wallsend Burn, contents of most heavy metals in soils from this area were lower.

Beta-Glucosidase Activity in Paddy Soils of the Taihu Lake Region, China

The profile distribution of β-gulcosidase activity in twelve typical paddy soil profiles with high productivity in the Taihu Lake region of China were investigated. Activities of β-gulcosidase in the plow layers were in the range of 52.68- 137.02μg PNP g^-1 soil h^-1 with a mean of 89.22μg PNP g^-1 soil h^-1. However, most plow layers ranged from 70 to 110 μg PNP g^-1 soil h^-1. The profile distribution of β-gulcosidase activity in the 12 soil profiles decreased rapidly with soil depth, with activity at the 60 cm depth only about 10% of that in the surface layers （0-15 cm or 0-20 cm）. In these soil profiles, β-gulcosidase activity was significantly positively correlated with soll organic carbon and arylsulphatase activity. Meanwhile, a significantly negative correlation was shown between β-gulcosidase activity and soil pH.

Effect of Nitrogen Fertilizers on Movement and Transformation of Phosphorus in an Acid Soil

The effects of two different nitrogen fertilizers （urea and NH4C1） with monocalcium phosphate （MCP） on the movement and transformation of fertilizer P in soil microsites along with soil pH changes at different distances from the fertilizer application site were studied in an incubation experiment. A highly acidic red soil （Ultisol, pH 4.57） from south China with MCP fertilizer alone or in combination with NH4C1 or urea was added to the surface of soil cylinders and packed in wax blocks. After 7 and 28 days, the extraction and analysis of each 2 mm layer from the interface of the soil and fertilizer showed that added NH4C1 or urea did not change the movement distance of fertilizer P. However, P transformation was significantly affected （P 〈 0.05）. After 7 days, at 0-8 mm distance from the fertilizer site the addition of urea significantly decreased the water-extractable P concentration; however, after 28 days the effect of N addition had disappeared. Also,at limited distances close to the fertilizer site NH4Cl application with MCP significantly increased acid-extractable P and available P, while with the addition of urea they significantly decreased. Compared with application of MCP alone,addition of urea significantly increased soil pH in fertilizer microsites, whereas the addition of NH4Cl significantly decreased soil pH.