Additive potential of ginger starch on antifungal potency of honey against Candida albicans

Objective:To evaluate the additive action of ginger starch on the antifungal activity of honey against Candida albicans(C.albicans).Methods:C.albicans was used to determine the minimum inhibitory concentration(MIC)of four varieties of Algerian honey.Lower concentrations of honey than the MIC were incubated with a set of concentrations of starch and then added to media to detennine the minimum additive inhibitory concentration(MAIC).Results:The MIC for the four varieties of honey without starch against C.albicans ranged between 38%and 42%(v/v).When starch was incubated with honey and then added to media,a MIC drop was noticed with each variety.MAIC of the four varieties ranged between 32%honey(v/v)with 4%starch and 36%honey(v/v) with 2%starch.Conclusions:The use of ginger starch allows honey benefit and will constitute an alternative way against the resistance to antifungal agents.

Antibacterial activity of various honey types of Algeria against Staphylococcus aureus and Streptococcus pyogenes

Objective:To assess the in vitro antibacterial activity of honey from different geographical location on Gram negative organismes.Methods:Different concentrations(Undiluted honey,10%, 30%,50%and 70%wt/vol) of honey were studied in vitro using Staphylococcus aureus(S.aureus) and Streptococcus pyogenes(S.pyogenes),briefly,two-fold dilutions of honey solutions were tested to determine the minimum inhibitory concentration(MIC) against each type of microorganism,followed by more assays within a narrower dilution range to obtain more precise MIC values.MICs were determined by both visual inspection and spectrophotometric assay at 620 nm.These honey samples were compared with standard antibiotics like ampicillin,penicillin G,amoxicillin,gentamycin,tobramycin,erythromycin and chloramphenicol was determined by the disc diffusion method.Results:The diameter of zone of the inhibition(ZDI) of honey has various concentrations tested for the isolates ranged 0-46 mm for S.aureus,0-44 mm for S.pyogenes.While the MIC(%) ranged 12%-95%,25%-73%respectively.Conclusions:Algeria honey,in-vitro,possess antibacterial activity.