Expression of toxin-related human mono-ADP-ribosyltransferase 3 in human testes

Aim： To investigate wether the corresponding protein of mono-ADP-ribosyltransferase 3 （ART3） mRNA is expressed in human testes and, if so, whether the expression is cell type-specific. Methods： ART3 mRNA was determined in human testes and sperm by reverse transcription-polymerase chain reaction （RT-PCR）. The glycosylphosphatidylinositol linkage of ART3 was shown by treating ART3-transfected HEK-293-T cells with phospholipase C. Fluorescent activated cell sorter （FACS）-analyses were used to detect ART3 on mature spermatozoa and immunohistological studies to detect the protein in testes. Results： ART3 protein was shown to be present in testes. It was found on spermatocytes only. It was absent from spermatogonia, spermatids and spermatozoa. The absence of ART3 from spermatozoa was confirmed by FACS-analysis. ART3 protein was detected neither within a seminoma nor on Leydig cells. Conclusion： Here we show for the first time that ART3 protein is expressed in testes in particular on spermatocytes, indicating that ART3 exerts a specific function only required at a particular stage of spermatogenesis.

Effect of Vasocation Intestinal Peptide on Immune Privilege of the Rat Testis

Objective To study the effect of vasocation intestinal peptide （VIP） on immune privilege of the rat testis. Methods The UU infected SD rats and Leydig cells were intervened by VIP, the secretion of TGF-β and the expression of FasL in rat Leydig cells were compared between VIP-intervened group and control group to test the effect of VIP on immune privilege of the rat testis in vitro and in vivo. Results In vitro, the secretion of TGF-β in Leydig cells could be increased by low dosage of VIP while inhibitited by high dosage of VIP; expression of FasL mRNA in Leydig cells could be decreased by VIP In vivo, increased expression of TGF-β mRNA and decreased FasL mRNA were observed in VIP group in 2-3 weeks after infected by UU. In addition, the apoptosis of Jurkat cells mediated by Leydig cells could be prevented by VIP Conclusion When Leydig cells or testis infected by UU, VIP could regulate the immune function of rat Leydig cells and participate in the regulation of immune privilege of testis through the regulation of TGF-β secretion and FasL expression pattern of Leydig cells.

MicroRNA let-7b targets important cell cycle molecules in malignant melanoma cells and interferes with anchorage-independent growth

一幅 microRNA 表示屏幕被执行从良性的 melanocytic nevi 在 laser-microdissected 纸巾分析 157 不同 microRNAs (n = 10 ) 并且主要恶意的黑瘤(n = 10 ) ，用量的即时 PCR。微分表示被作出对有利的裁决 72 microRNAs。microRNAs 的 let-7 家庭的成员是显著地， down 作为与良性的 nevi 相比在主要黑瘤调整了，为是的这些分子的一个可能的角色暗示肿瘤在恶意的黑瘤压制 ors。有趣地，类似的调查结果为肺和结肠癌被描述了。在黑瘤房间试管内 down 的 let-7b 的 Overexpression 调整了 cyclins 的表示 D1， D3，和 A，和 cyclin 依赖的 kinase (Cdk ) 4，所有哪个被描述了在黑瘤开发起一个作用。蛋白质表示上的 let-7b 的效果由于单个 mRNAs 的 3'-untranslated 区域(3'UTRs ) 指向，为 cyclin D1 由记者基因分析例示。与它细胞周期管理者上的 down modulating 效果一致， let-7b 禁止了细胞周期前进和黑瘤细胞的抛锚无关的生长。总起来说，这些调查结果不仅指向早黑瘤开发的新规章的机制，而且可以打开大街因为未来指向了这个肿瘤的治疗。

Bone marrow-derived pancreatic stellate cells in rats

胰腺的星形的房间(PSC ) 在前，在期间并且在胰腺的损害以后的起源和命运是争论的一件事。在胰腺的纤维变性的致病的 PSC 的关键角色通常被接受。然而，房间的周转仍然保持阴暗。现在的学习处理了潜在的骨头髓(BM ) 的问题 PSC 的起源。我们由 grafting 使用了稳定的造血的 chimerism 的一个模型在领受人老鼠的照耀以后表示 BM 房间的提高的绿荧光蛋白质(eGFP ) 。Chimerism 被 eGFP 积极的房间的 FACS 分析在外部血检测。Dibutyltin 二氯化物(DBTC ) 被用来超过 4 个星期与随后的恢复导致尖锐胰腺的发炎。调查集中于孤立的房间检测休息 PSC 人口。从妄想的老鼠的胰获得的 eGFP 积极的 PSC 的发生在健康胰腺的织物是约 7% 并且 4 个星期在导致 DBTC 的尖锐发炎以后在恢复的胰显著地增加了到 18% 的一个平均数。我们的结果建议导出 BM 的祖先房间在胰代表可更新的星形的房间的来源。在新生以后放松 PSC 的增加的数字在导致发炎的激活以后向导出 BM 的房间的提高的招募指向一个静止状态的胰或重新获得。

Receptor-mediated gene delivery using polyethylenimine (PEI) coupled with polypeptides targeting FGF receptors on cells surface

Objective: To construct a novel kind of nonviral gene delivery vector based on polyethylenimine (PEI) conjugated with polypeptides derived from ligand FGF with high transfection efficiency and according to tumor targeting ability. Methods: The synthetic polypeptides CR16 for binding FGF receptors was conjugated to PEI and the characters of the polypeptides in-cluding DNA condensing and particle size were determined. Enhanced efficiency and the targeting specificity of the synthesized vector were investigated in vitro and in vivo. Results: The polypeptides were successfully coupled to PEI. The new vectors PEI-CR16 could efficiently condense pDNA into particles with around 200 nm diameter. The PEI-CR16/pDNA polyplexes showed significantly greater transgene activity than PEI/pDNA in FGF receptors positive tumor cells in vitro and in vivo gene transfer, while no difference was observed in FGF receptors negative tumor cells. The enhanced transfection efficiency of PEI-CR16 could be blocked by excess free polypeptides. Conclusion: The synthesized vector could improve the efficiency of gene transfer and targeting specificity in FGF receptors positive cells. The vector had good prospect for use in cancer gene therapy.