Molecular phylogenetic relationships based on mitochondrial genomes of novel deep-sea corals(Octocorallia:Alcyonacea):Insights into slow evolution and adaptation to extreme deep-sea environments

A total of 10 specimens of Alcyonacea corals were collected at depths ranging from 905 m to 1633 m by the manned submersible Shenhai Yongshi during two cruises in the South China Sea(SCS).Based on mitochondrial genomic characteristics,morphological examination,and sclerite scanning electron microscopy,the samples were categorized into four suborders(Calcaxonia,Holaxonia,Scleraxonia,and Stolonifera),and identified as 9 possible new cold-water coral species.Assessments of GC-skew dissimilarity,phylogenetic distance,and average nucleotide identity(ANI)revealed a slow evolutionary rate for the octocoral mitochondrial sequences.The nonsynonymous(Ka)to synonymous(Ks)substitution ratio(Ka/Ks)suggested that the 14 protein-coding genes(PCGs)were under purifying selection,likely due to specific deep-sea environmental pressures.Correlation analysis of the median Ka/Ks values of five gene families and environmental factors indicated that the genes encoding cytochrome b(cyt b)and DNA mismatch repair protein(mutS)may be influenced by environmental factors in the context of deep-sea species formation.This study highlights the slow evolutionary pace and adaptive mechanisms of deep-sea corals.

Analysis of environmental selection pressure of superoxide dismutase in deep-sea sea cucumber

Manganese superoxide dismutase(MnSOD)is an antioxidant that exists in mitochondria and can effectively remove superoxide anions in mitochondria.In a dark,high-pressure,and low-temperature deep-sea environment,MnSOD is essential for the survival of sea cucumbers.Six MnSODs were identified from the transcriptomes of deep and shallow-sea sea cucumbers.To explore their environmental adaptation mechanism,we conducted environmental selection pressure analysis through the branching site model of PAML software.We obtained night positive selection sites,and two of them were significant(97F→H,134K→V):97F→H located in a highly conservative characteristic sequence,and its polarity c hange might have a great impact on the function of MnSOD;134K→V had a change in piezophilic a bility,which might help MnSOD adapt to the environment of high hydrostatic pressure in the deepsea.To further study the effect of these two positive selection sites on MnSOD,we predicted the point mutations of F97H and K134V on shallow-sea sea cucumber by using MAESTROweb and PyMOL.Results show that 97F→H,134K→V might improve MnSOD’s efficiency of scavenging superoxide a nion and its ability to resist high hydrostatic pressure by moderately reducing its stability.The above results indicated that MnSODs of deep-sea sea cucumber adapted to deep-sea environments through their amino acid changes in polarity,piezophilic behavior,and local stability.This study revealed the correlation between MnSOD and extreme environment,and will help improve our understanding of the organism’s adaptation mechanisms in deep sea.

Geometry and 3D seismic characterisation of post-rift normal faults in the Pearl River Mouth Basin,northern South China Sea

Based on high-resolution 3D seismic data acquired in the Pearl(Zhujiang)River Mouth Basin of the northern South China Sea,this study investigated the geometry,spatial extension,and throw distribution of the post-rift normal fault through detailed seismic interpretation and fault modeling.A total of 289 post-rift normal faults were identified in the study area and can be classified into four types:(1)isolated normal faults above the carbonate platform;(2)isolated normal faults cutting through the carbonate platform;(3)conjugate normal faults,and(4)connecting normal faults.Throw distribution analysis on the fault planes show that the vertical throw profiles of most normal fault exhibit flat-topped profiles.Isolated normal faults above the carbonate platform exhibit roughly concentric ellipses with maximum throw zones in the central section whereas the normal faults cutting through the carbonate platform miss the lowermost section due to the chaotic seismic reflections in the interior of the carbonate platform.The vertical throws of conjugate normal faults anomalously decrease toward their intersection region on the fault plane whereas the connecting normal faults present two maximum throw zones in the central section of the fault plane.According to the symmetric elliptical distribution model of fault throw,an estimation was made indicating that normal faults cutting through the carbonate platform extended downward between-1308 s and-1780 s(two-way travel time)in depth and may not penetrate the entire Liuhua carbonate platform.Moreover,it is observed that the distribution of karst caves on the top of the carbonate platform disaccord with those of hydrocarbon reservoirs and the post-rift normal faults cutting through the carbonate platform in the study area.We propose that these karst caves formed most probably by corrosive fluids derived from magmatic activities during the Dongsha event,rather than pore waters or hydrocarbons.

Westerlies Affecting the Seasonal Variation of Water Vapor Transport over the Tibetan Plateau Induced by Tropical Cyclones in the Bay of Bengal

This study investigates the activity of tropical cyclones(TCs)in the Bay of Bengal(BOB)from 1979 to 2018 to discover the mechanism affecting the contribution rate to the meridional moisture budget anomaly(MMBA)over the southern boundary of the Tibetan Plateau(SBTP).May and October–December are the bimodal phases of BOB TC frequency,which decreases month by month from October to December and is relatively low in May.However,the contribution rate to the MMBA is the highest in May.The seasonal variation in the meridional position of the westerlies is the key factor affecting the contribution rate.The relatively southern(northern)position of the westerlies in November and December(May)results in a lower(higher)contribution rate to the MMBA.This mechanism is confirmed by the momentum equation.When water vapor enters the westerlies near the trough line,the resultant meridional acceleration is directed north.It follows that the farther north the trough is,and the farther north the water vapor can be transported.When water vapor enters the westerlies from the area near the ridge line,for Type-T(Type-R)TCs,water vapor enters the westerlies downstream of the trough(ridge).Consequently,the direction of the resultant meridional acceleration is directed south and the resultant zonal acceleration is directed east(west),which is not conducive to the northward transport of water vapor.This is especially the case if the trough or ridge is relatively south,as the water vapor may not cross the SBTP.

A triboelectric nanogenerator based on a spiral rotating shaft for efficient marine energy harvesting of the hydrostatic pressure differential

Equipment used in underwater sensing and exploration typically relies on cables or batteries for energy supply,resulting in a limited and inconvenient energy supply and marine environmental pollution that hinder the sustainable development of distributed ocean sensing networks.Here,we design a deep-sea differential-pressure triboelectric nanogenerator(DP-TENG)based on a spiral shaft drive using modified polymer materials to harness the hydrostatic pressure gradient energy at varying ocean depths to power underwater equipment.The spiral shaft structure converts a single compression into multiple rotations of the TENG rotor,achieving efficient conversion of differential pressure energy.The multi-pair electrode design enables the DP-TENG to generate a peak current of 61.7μA,the instantaneous current density can reach 0.69μA cm^(-2),and the output performance can be improved by optimizing the spiral angle of the shaft.The DP-TENG can charge a 33μF capacitor to 17.5 V within five working cycles.It can also power a digital calculator and light up 116 commercial power light-emitting diodes,demonstrating excellent output capability.With its simple structure,low production cost,and small form factor,the DP-TENG can be seamlessly integrated with underwater vehicles.The results hold broad prospects for underwater blue energy harvesting and are expected to contribute to the development of self-powered equipment toward emerging“smart ocean”and blue economy applications.

Deep-Sea Geohazards in the South China Sea

Various geological processes and features that might inflict hazards identified in the South China Sea by using new technologies and methods.These features include submarine landslides,pockmark fields,shallow free gas,gas hydrates,mud diapirs and earthquake tsunami,which are widely distributed in the continental slope and reefal islands of the South China Sea.Although the study and assessment of geohazards in the South China Sea came into operation only recently,advances in various aspects are evolving at full speed to comply with National Marine Strategy and‘the Belt and Road’Policy.The characteristics of geohazards in deep-water seafloor of the South China Sea are summarized based on new scientific advances.This progress is aimed to aid ongoing deep-water drilling activities and decrease geological risks in ocean development.

The pressure compensation technology of deep-sea sampling based on the real gas state equation

Compressed gas is usually used for the pressure compensation of the deep-sea pressure-maintaining sampler.The pressure and volume of the recovered fluid sample are highly related to the precharged gas. To better understand the behavior of the gas under high pressure, we present a new real gas state equation based on the compression factor Z which was derived from experimental data. Then theoretical calculation method of the pressure and volume of the sample was introduced based on this empirical gas state equation. Finally, the proposed calculation method was well verified by the high-pressure vessel experiment of the sampler under 115 MPa.

Distinct influence of trimethylamine N-oxide and high hydrostatic pressure on community structure and culturable deep-sea bacteria

Trimethylamine N-oxide(TMAO)is one of the most important nutrients for bacteria in the deep-sea environment and is capable of improving pressure tolerance of certain bacterial strains.To assess the impact of TMAO on marine microorganisms,especially those dwelling in the deep-sea environment,we analyzed the bacterial community structure of deep-sea sediments after incubated under different conditions.Enrichments at 50 MPa and 0.1 MPa revealed that TMAO imposed a greater influence on bacterial diversity and community composition at atmospheric pressure condition than that under high hydrostatic pressure(HHP).We found that pressure was the primary factor that determines the bacterial community.Meanwhile,in total,238 bacterial strains were isolated from the enrichments,including 112 strains a ffiliated to 16 genera of 4 phyla from the Yap Trench and 126 strains a ffiliated to 11 genera of 2 phyla from the Mariana Trench.Treatment of HHP reduced both abundance and diversity of isolates,while the presence of TMAO mainly af fected the diversity of isolates obtained.In addition,certain genera were isolated only when TMAO was supplemented.Taken together,we demonstrated that pressure primarily defines the bacterial community and culturable bacterial isolates.Furthermore,we showed for the first time that TMAO had distinct influences on bacterial community depending on the pressure condition.The results enriched the understanding of the significance of TMAO in bacterial adaptation to the deep-sea environment.

Acoustics-Based Autonomous Docking for A Deep-Sea Resident ROV

This paper presents autonomous docking of an inhouse built resident Remotely Operated Vehicle(ROV),called Rover ROV,through acoustic guided techniques.A novel cage-type docking station has been developed.The docking station can be placed on a deep-sea lander,taking the Rover ROV to the seafloor.Instead of using vision-based pose estimation techniques and expensive navigation sensors,the Rover ROV docking adopts an ultra-short baseline(USBL)and low-cost inertial sensors to build an adaptive fault-tolerant integrated navigation system.To solve the problem of sonar-based failure positioning,the measurement residuals are exploited to detect measurement faults.Then,an adaptation scheme for estimating the statistical characteristics of noise in real-time is proposed,which can provide robust and smooth positioning results.It is more suitable for a compact and low-cost deep-sea resident ROV.Field experiments have been conducted successfully in the Qiandao Lake and the South China Sea area with a depth of 3000 m,respectively.The experimental results show that the functionality of autonomous docking has been achieved.Under the guidance of the navigation system,the Rover ROV can autonomously and efficiently return to the docking station within a range of 100 m even when the amounts of outliers exist in the acoustic positioning data.These achievements can be applied to current ROVs by an easy retrofit.

Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

Trimethylamine N-oxide(TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from dif ferent genera harbor high hydrostatic pressure(HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from ?sh which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affliated to 23 genera of Proteobacteria,Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at dif ferent depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas,Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-speci?c feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the ?rst time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.

Positive selection analysis reveals the deep-sea adaptation of a hadal sea cucumber ( Paelopatides sp.) to the Mariana Trench

The Mariana Trench,the deepest trench on the earth,is ideal for deep-sea adaptation research due to its unique characters,such as the highest hydrostatic pressure on the Earth,constant ice-cold temperature,and eternal darkness.In this study,tissues of a the hadal holothurian(Paelopatides sp.)were fi xed with RNA later in situ at~6501-m depth in the Mariana Trench,which,to our knowledge,is the deepest in-situ fi xed animal sample.A high-quality transcript was obtained by de-novo transcriptome assembly.A maximum likelihood tree was constructed based on the single copy orthologs across nine species with their available omics data.To investigate deep-sea adaptation,113 positively selected genes(PSGs)were identifi ed in Paelopatides sp.Some PSGs such as microphthalmia-associated transcription factor(MITF)may contribute to the distinct phenotype of Paelopatides sp.,including its translucent white body and degenerated ossicles.At least eight PSGs(transcription factor 7-like 2[TCF7L2],ETS-related transcription factor Elf-2-like[ELF2],PERQ amino acid-rich with GYF domain-containing protein[GIGYF],cytochrome c oxidase subunit 7a,[COX7A],type I thyroxine 5′-deiodinase[DIO1],translation factor GUF1[GUF1],SWI/SNF related-matrix-associated actin-dependent regulator of chromatin subfamily C and subfamily E,member 1[SMARCC]and[SMARCE1])might be related to cold adaptation.In addition,at least nine PSGs(cell cycle checkpoint control protein[RAD9A],replication factor A3[RPA3],DNA-directed RNA polymerases I/II/III subunit RPABC1[POLR2E],putative TAR DNA-binding protein 43 isoform X2[TARDBP],ribonucleoside-diphosphate reductase subunit M1[RRM1],putative serine/threonine-protein kinase[SMG1],transcriptional regulator[ATRX],alkylated DNA repair protein alkB homolog 6[ALKBH6],and PLAC8 motif-containing protein[PLAC8])may facilitate the repair of DNA damage induced by the high hydrostatic pressure,coldness,and high concentration of cadmium in the upper Mariana Trench.

In situ cultivation of deep-sea water with bicarbonate fueled a different microbial community

Some deep-sea microbes may incorporate inorganic carbon to reduce CO_(2) emission to upper layer and atmosphere.How the microbial inhabitants can be affected under addition of bicarbonate has not been studied using in situ fixed and lysed samples.In this study,we cultivated 40 L natural bottom water at~1000 m depth with a final concentration of 0.1 mmol/L bicarbonate for 40 min and applied multiple in situ nucleic acids collection(MISNAC)apparatus for nucleic acids extraction from the cultivation.Our classification result of the cultivation sample showed a distinct microbial community structure,compared with the samples obtained by Niskin bottle and six working units of MISNAC.Except for notable enrichment of Alteromonas,we detected prevalence of Asprobacter,Ilumatobacter and Saccharimonadales in the cultivation.Deep-sea lineages of Euryarchaeota,SAR406,SAR202 and SAR324 were almost completely absent from the cultivation and Niskin samples.This study revealed the dominant microbes affected by bicarbonate addition and Niskin sampling,which suggested rapid responses of deep-sea microbes to the environmental changes.

Slope Instability Analysis of the Qiongdongnan Basin in the Northern Part of the South China Sea:Implications for the Risk Evaluation of Deepwater Drilling

The instability of continental slopes damages marine engineering equipment,such as submarine pipelines,resulting in the generation of tsunamis,which endangers the safety of nearshore personnel.Therefore,research on the instability of continental slopes where submarine landslides usually occur is crucial to the risk evaluation of deepwater drilling.Previous studies were mainly based on simplified 2D and 3D models,which extend the 2D model applied on submarine slopes with complex topography.In this study,a numerical model with bathymetric data from the Qiongdongnan Basin was established.Furthermore,3D slope stability analysis and static and dynamic analyses were conducted.The static analysis found two discussions where slopes are most likely to occur.Through the analysis of different seismic forces,the dynamic result showed that an instability area is added to the two positions where the static analysis is unstable.Topography scatters and transmits seismic waves and controls the accumulation and diffusion of seismic energy.3D calculations and analysis revealed that the direction of slope instability is closely related to terrain inclination,slope,terrain effect,and terrain curvature.Data showed that instability situations could not be derived from a single direction or profile data.Such situations are an important factor in slope stability analysis and are critical to the prediction and evaluation of marine geological disasters.

Well logging evaluation of fine-grained hydrate-bearing sediment reservoirs: Considering the effect of clay content

Hydrate reservoirs are different from the host reservoirs of all other fossil energy sources because the characteristics of hydrate reservoirs are generally controlled by deep-sea fine-grained sedimentation. In such reservoirs, the reliability of the classical logging evaluation models established for diagenetic reservoirs is questionable. This study used well W8 in the Qiongdongnan Basin to explore the clay content, porosity, saturation, and hydrate-enriched layer identification of a logging-based hydrate reservoir, and it was found that considering the effect of the clay content on the log response is necessary in the logging evaluation of hydrate reservoirs. In the evaluation of clay content, a method based on the optimization inversion method can obtain a more reliable clay content than other methods. Fine-grained sediment reservoirs have a high clay content, and the effect of clay on log responses must be considered when calculating porosity. In addition, combining density logging and neutron porosity logging data can obtain the best porosity calculation results, and the porosity calculation method based on sonic logging predicted that the porosity of the studied reservoir was low. It was very effective to identify hydrate layers based on resistivity, but the clay distribution and pore structure will also affect the relationship between resistivity, porosity and saturation, and it was suggested that the factors effecting the resistivity of different layers should be considered in the saturation evaluation and that a suitable model should be selected. This study also considered the lack of clarity of the relationships among the lithology, physical properties, hydrate-bearing occurrence properties, and log response properties of hydrate reservoirs and the lack of specialized petrophysical models. This research can directly help to improve hydrate logging evaluation.

Total organic carbon content logging prediction based on machine learning:A brief review

The total organic carbon content usually determines the hydrocarbon generation potential of a formation.A higher total organic carbon content often corresponds to a greater possibility of generating large amounts of oil or gas.Hence,accurately calculating the total organic carbon content in a formation is very important.Present research is focused on precisely calculating the total organic carbon content based on machine learning.At present,many machine learning methods,including backpropagation neural networks,support vector regression,random forests,extreme learning machines,and deep learning,are employed to evaluate the total organic carbon content.However,the principles and perspectives of various machine learning algorithms are quite different.This paper reviews the application of various machine learning algorithms to deal with total organic carbon content evaluation problems.Of various machine learning algorithms used for TOC content predication,two algorithms,the backpropagation neural network and support vector regression are the most commonly used,and the backpropagation neural network is sometimes combined with many other algorithms to achieve better results.Additionally,combining multiple algorithms or using deep learning to increase the number of network layers can further improve the total organic carbon content prediction.The prediction by backpropagation neural network may be better than that by support vector regression;nevertheless,using any type of machine learning algorithm improves the total organic carbon content prediction in a given research block.According to some published literature,the determination coefficient(R^(2))can be increased by up to 0.46 after using machine learning.Deep learning algorithms may be the next breakthrough direction that can significantly improve the prediction of the total organic carbon content.Evaluating the total organic carbon content based on machine learning is of great significance.

Benthodytes tetrapapillata sp.nov.,a new elasipodid sea cucumber(Elasipodida:Psychropotidae)from a seamount in the Western Pacific Ocean

Benthodytes tetrapapillata sp.nov.was collected from a seamount located on the Caroline Ridge at a depth of 2289 m,during the cruise of R/V Kexue in June 2019.We provided detailed descriptions of external and deposits morphology.The phylogenetic analyses based on cytochrome c oxidase I(COI)and a concatenated dataset of 16S and COI genes showed that the new species belonged to Benthodytes that is not monophyletic.Both details of morphological comparisons and molecular analyses confirmed that Benthodytes tetrapapillata sp.nov.is a new psychropotid species.A state of main morphological characters in valid species of Benthodytes is also provided in this study.

A SiC asymmetric cell trench MOSFET with a split gate and integrated p^(+)-poly Si/SiC heterojunction freewheeling diode

A new SiC asymmetric cell trench metal–oxide–semiconductor field effect transistor(MOSFET)with a split gate(SG)and integrated p^(+)-poly Si/SiC heterojunction freewheeling diode(SGHJD-TMOS)is investigated in this article.The SG structure of the SGHJD-TMOS structure can effectively reduce the gate-drain capacitance and reduce the high gateoxide electric field.The integrated p^(+)-poly Si/SiC heterojunction freewheeling diode substantially improves body diode characteristics and reduces switching losses without degrading the static characteristics of the device.Numerical analysis results show that,compared with the conventional asymmetric cell trench MOSFET(CA-TMOS),the high-frequency figure of merit(HF-FOM,R_(on,sp)×Q_(gd,sp))is reduced by 92.5%,and the gate-oxide electric field is reduced by 75%.In addition,the forward conduction voltage drop(V_(F))and gate-drain charge(Q_(gd))are reduced from 2.90 V and 63.5μC/cm^(2) in the CA-TMOS to 1.80 V and 26.1μC/cm^(2) in the SGHJD-TMOS,respectively.Compared with the CA-TMOS,the turn-on loss(E_(on)) and turn-off loss(E_(off)) of the SGHJD-TMOS are reduced by 21.1%and 12.2%,respectively.

Revisiting the Seasonal Evolution of the Indian Ocean Dipole from the Perspective of Process-Based Decomposition

The seasonal phase-locking feature of the Indian Ocean Dipole(IOD)is well documented.However,the seasonality ten-dency of sea surface temperature anomalies(SSTAs)during the development of the IOD has not been widely investigated.The SSTA tendencies over the two centers of the IOD peak in September-October-November are of different monthly amplitudes.The SSTA tendency over the west pole is small before June-July-August but dramatically increases in July-August-September.Meanwhile,the SSTA tendency over the east pole gradually increases before June-July-August and decreases since then.The growth rate attribution of the SSTAs is achieved by examining the roles of radiative and non-radiative air-sea coupled thermodynamic processes through the climate feedback-response analysis method(CFRAM).The CFRAM results indicate that oceanic dynamic processes largely contribute to the total SSTA tendency for initiating and fueling the IOD SSTAs,similar to previous studies.However,these results cannot ex-plain the monthly amplitudes of SSTA tendency.Four negative feedback processes(cloud radiative feedback,atmospheric dynamic processes,surface sensible,and latent heat flux)together play a damping role opposite to the SSTA tendency.Nevertheless,the sea surface temperature-water vapor feedback shows positive feedback.Specifically,variations in SSTAs can change water vapor con-centrations through evaporation,resulting in anomalous longwave radiation that amplifies the initial SSTAs through positive feedback.The effect of water vapor feedback is well in-phase with the monthly amplitudes of SSTA tendency,suggesting that the water vapor feedback might modulate the seasonally dependent SSTA tendency during the development of the IOD.

Extensional structures of the Nan'an Basin in the rifting tip of the South China Sea: Implication for tectonic evolution of the southwestern continental margin

Nan'an Basin is a giant hydrocarbon basin,but its tectonic division scheme and associated fault systems has not been well understood.Based on newly acquired seismic data from the southwestern margin of the South China Sea,this study analyzed the structural units,tectonic feature and geodynamics of the sedimentary basin.The new data suggests that the Nan0 an Basin is a rift basin oriented in the NE-SW direction,rather than a pull-apart basin induced by strike-slip faults along the western margin.The basin is a continuation of the rifts in the southwest South China Sea since the late Cretaceous.It continued rifting until the middle Miocene,even though oceanic crust occurred in the Southwest Subbasin.However,it had no transfer surface at the end of spreading,where it was characterized by a late middle Miocene unconformity(reflector T3).The Nan'an Basin can be divided into eight structural units by a series of NE-striking faults.This study provides evidences to confirm the relative importance and interplay between regional strike-slips and orthogonal displacement during basin development and deformation.The NE-SW-striking dominant rift basin indicates that the geodynamic drivers of tectonic evolution in the western margin of the South China Sea did not have a large strike-slip mechanism.Therefore,we conclude that a large strike-slip fault system did not exist in the western margin of the South China Sea.

Variation in concentration of dissolved silicate in the Eastern Philippine deep sea

Upper Circumpolar Deep Water(UCDW)and North Pacifi c Deep Water(NPDW)coexist in the upper deep layer(i.e.,with a 1.2-2.0-℃potential temperature range and a 2000-4100-dbar pressure range)of the Eastern Philippine Sea.They have similar properties in potential temperature and salinity,while have a signifi cant diff erence in dissolved silicate.Based on the repeated observations along a 137°E transect from the World Ocean Database(WOD18),this study revealed the interannual variability of dissolved silicate in the upper deep layer of the Eastern Philippine Sea.Dissolved silicate increased in 1995,1996,2005,2006,and 2007,and decreased in 1997,2000,2001,2002,and 2004.Composition analysis showed that the large diff erence between positive and negative dissolved silicate anomalies occurred mainly at~15°N and north of 25°N,with the concentration reaching 4.25μmol/g.Further analysis indicated that the interannual dissolved silicate variability was related to the zonal current variation in the upper deep layer.The relatively strong(weak)westward current transport increased(decreased)NPDW to the Eastern Philippine Sea,thereby resulting in increased(decreased)dissolved silicate.