Vaccination interventions is consideredan important preventive measure to block the transmission of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and protect the organism from pathogen infection effective...Vaccination interventions is consideredan important preventive measure to block the transmission of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and protect the organism from pathogen infection effectively.However,a quick and accurate technique to evaluate the immune efficacy of the SARS-CoV-2 inactivated vaccine remains scarce.In this paper,an IgM-IgG antibody combined detection colloidal gold immunochromatography assay kit was optimized and developed,which can assess the efficacy of the inactivated SARS-CoV-2 vaccine.We collected fingertip blood samples from 3 vaccinees and 1 unvaccinated sample.The results showed that the proportion of antibody was high after the second shots immunization.The colloidal gold-based immunochromatographic strip is rapid,convenient and easy to operate.It can be used as an auxiliary method for preliminary evaluation of the antibody effect of vaccine recipients,and provide a reference index for the potential clinical application value of the vaccine.展开更多
基金This work was supported by the National Natural Science Foundation of China[grant number 81970186].
文摘Vaccination interventions is consideredan important preventive measure to block the transmission of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and protect the organism from pathogen infection effectively.However,a quick and accurate technique to evaluate the immune efficacy of the SARS-CoV-2 inactivated vaccine remains scarce.In this paper,an IgM-IgG antibody combined detection colloidal gold immunochromatography assay kit was optimized and developed,which can assess the efficacy of the inactivated SARS-CoV-2 vaccine.We collected fingertip blood samples from 3 vaccinees and 1 unvaccinated sample.The results showed that the proportion of antibody was high after the second shots immunization.The colloidal gold-based immunochromatographic strip is rapid,convenient and easy to operate.It can be used as an auxiliary method for preliminary evaluation of the antibody effect of vaccine recipients,and provide a reference index for the potential clinical application value of the vaccine.
