Effect of systemic intravenous chemotherapy combined with regional arterial perfusion chemoembolization on the malignancy of locally advanced gastric cancer

Objective:To study the effect of systemic intravenous chemotherapy combined with regional arterial perfusion chemoembolization on the malignancy of locally advanced gastric cancer. Methods:A total of 90 patients with primary gastric cancer who received treatment in Tianyou Hospital Affiliated to Wuhan University of Science & Technology between January 2014 and May 2016 were collected and divided into control group and observation group according to the random number table method, 45 cases in each group. The control group of patients received routine systemic intravenous chemotherapy + surgical treatment, and the observation group of patients received systemic intravenous chemotherapy combined with local arterial perfusion chemoembolization + surgical treatment. Levels of tumor markers and angiogenesis factors in serum as well as the expression of oncogenes and tumor suppressor genes in gastric cancer tissue were compared between the two groups of patients before and after chemotherapy.Results:Before chemotherapy, the levels of tumor markers and angiogenesis factors in serum as well as the expression of oncogenes and tumor suppressor genes in gastric cancer tissue were not significantly different between the two groups of patients;after chemotherapy, serum CEA, CA724, CA242, AFP, VEGF, Ang-2, COX2 and PD-ECGF levels of observation group were lower than those of control group, andiASPP, p130Cas, ERBB2 and C-myc mRNA expression in gastric cancer tissue were lower than those of control group while GKN1, p16, PTEN, TSPYL5 and merlin mRNA expression in gastric cancer tissue were higher than those of control group.Conclusions: Preoperative systemic intravenous chemotherapy combined with regional arterial perfusion chemoembolization can effectively reduce the malignancy of locally advanced gastric cancer and provide favorable conditions for the operation.

Correlation of apoptosis inducer TWEAK and extracellular matrix protein Del-1 with pancreatic cancer malignancy

Objective: To study the correlation of apoptosis inducer TWEAK and extracellular matrix protein Del-1 with pancreatic cancer malignancy. Methods: A total of 54 patients with pancreatic cancer who received radical resection and 32 patients with pancreatic trauma who received partial resection in Tianyou Hospital Affiliated to Wuhan University of Science &Technology between May 2014 and March 2017 were selected, proper amount of pancreatic cancer tissue and normal pancreatic tissue were collected respectively, RNA was extracted and synthesized into cDNA by reverse transcription, and then fluorescence quantitative PCR kits were used to determine TWEAK, Del-1, Survivin, USP9X, DPF2, Stathmin, CD44v9, GPSM2, MMP9, Snail and Vimentin mRNA expression. Results: TWEAK, Del-1, Survivin, USP9X, DPF2, Stathmin, CD44v9, GPSM2, MMP9, Snail and Vimentin mRNA expression in pancreatic cancer tissue were significantly higher than those in normal pancreatic tissue;Survivin, USP9X, DPF2 and Stathmin mRNA expression in pancreatic cancer tissue with high TWEAK expression were significantly higher than those in pancreatic cancer tissue with low TWEAK expression;CD44v9, GPSM2, MMP9, Snail and Vimentin mRNA expression in pancreatic cancer tissue with high Del-1 expression were significantly higher than those in pancreatic cancer tissue with low Del-1 expression. Conclusion: The high expression of TWEAK and Del-1 in pancreatic cancer tissue can promote the proliferation and invasion of pancreatic cancer cells respectively.